gov.noaa.nmfs.inport:12673
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dataset
CSV Files
Alaska Fisheries Science Center
7600 Sand Point Way N.E., Building 4
Seattle
WA
98115
USA
http://www.afsc.noaa.gov
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Website
Website for this organization
information
0700-1700 Pacific Time
resourceProvider
Bahl, Kimberly
206 526 4314
kimberly.bahl@noaa.gov
email
pointOfContact
2024-02-29T00:00:00
ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data
ISO 19115-2:2009(E)
AFSC/RACE/EcoFOCI: NPRB project number 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flow cytometry cell cycle analysis
AFSC/RACE/EcoFOCI: NPRB project 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flo
publication
NOAA/NMFS/EDM
12673
https://www.fisheries.noaa.gov/inport/item/12673
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Full Metadata Record
View the complete metadata record on InPort for more information about this dataset.
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tableDigital
Walleye pollock are an important component of the eastern Bering Sea ecosystem due to their vast numbers and biomass and are of great commercial importance. Their recruitment in the eastern Bering Sea is not well understood but it is largely determined during the first year, so the larval stage may play a significant role. Starvation may contribute to the high and variable mortality that occurs during the larval stage so it is important to monitor and quantify it. This metadata describes data from laboratory and field studies conducted from 2009 to 2012 for NPRB project 926. The project completed the development of an assay using flow cytometric cell cycle analysis to assess the physiological condition of walleye pollock larvae, and applied it to field collected specimens. The use of RNA to improve the accuracy of the assay was also investigated, as well as the relationship between flow cytometric measurements and growth.
Results showed that the overall classification accuracy of the assay (healthy or unhealthy) ranged between 75 and 83% depending on the type of cross-validation testing. A nuclear RNA variable (the ratio of the number of S phase nuclei to the number of high nuclear RNA content G1 phase nuclei) improved overall classification accuracy by 11% compared to a model without it. Unhealthy walleye pollock larvae were present in the southeastern Bering Sea in 2009, and this was most likely the result of cold environmental conditions affecting prey availability and larval feeding. In 2010, no unhealthy larvae were detected. This may be the result of less spatial coverage and smaller collections of larvae than in 2009, given that the environmental conditions were similar between years.
To complete the development of an assay to assess the physiological condition of walleye pollock larvae using flow cytometric cell cycle analysis, and to apply it to field collected .
completed
Bahl, Kimberly
206 526 4314
kimberly.bahl@noaa.gov
email
pointOfContact
Porter, Steve
steve.porter@noaa.gov
email
custodian
notPlanned
Non-Federal Funding
theme
PARR Exclusion
flow cytometry
larvae
physiological condition
theme
Alaska
Bering Sea
place
DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce
dataCentre
Global Change Master Directory (GCMD) Data Center Keywords
2017-04-24
publication
8.5
Recruitment Processes Program
project
InPort
otherRestrictions
Cite As: Alaska Fisheries Science Center, [Date of Access]: AFSC/RACE/EcoFOCI: NPRB project number 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flow cytometry cell cycle analysis [Data Date Range], https://www.fisheries.noaa.gov/inport/item/12673.
NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
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Access Constraints: Contact Point Of Contact for data request form.
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Use Constraints: User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement
of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested.
unclassified
NOAA Data Management Plan (DMP)
NOAA/NMFS/EDM
12673
https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/12673.pdf
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NOAA Data Management Plan (DMP)
NOAA Data Management Plan for this record on InPort.
information
crossReference
eng; US
biota
Excel
-171.1
-163.3
54.3
57.4
Bering Sea
| Currentness: Ground Condition
2009
2012
CSV - Comma Separated Values (Text)
Porter, Steve
steve.porter@noaa.gov
email
distributor
https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/RPP/NPRB%20926%3B%20Results%20from%202009%20laboratory%20experiment%20measuring%20nuclear%20RNA.csv
WWW:LINK-1.0-http--link
https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/RPP/NPRB%20926%3B%20Results%20from%202009%20laboratory%20experiment%20measuring%20nuclear%20RNA.csv
Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/14/2021. Contact: Daniel.woodrich@noaa.gov
download
dataset
Completeness Report
Not applicable.
PORTER, S. M., and K. M. BAILEY. 2013. Using measurements of muscle cell nuclear
RNA with flow cytometry to improve assessment of larval condition of walleye
pollock (Gadus chalcogrammus). Fish. Bull., U.S. 111:337-351.
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otherRestrictions
Access Constraints: none | Use Constraints: none