AFSC/RACE/EcoFOCI: NPRB project number 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flow cytometry cell cycle analysis
Alaska Fisheries Science Center
Data Set
(DS)
| ID: 12673
| Published / External
Created: 2012-07-23
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Last Modified: 2023-10-17
Project (PRJ) | ID: 22352
ID: 12673
Data Set (DS)
* Discovery• First Pass
» Metadata Rubric
Item Identification
* » Title | AFSC/RACE/EcoFOCI: NPRB project number 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flow cytometry cell cycle analysis |
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Short Name | AFSC/RACE/EcoFOCI: NPRB project 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flo |
* Status | Completed |
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* » Abstract |
Walleye pollock are an important component of the eastern Bering Sea ecosystem due to their vast numbers and biomass and are of great commercial importance. Their recruitment in the eastern Bering Sea is not well understood but it is largely determined during the first year, so the larval stage may play a significant role. Starvation may contribute to the high and variable mortality that occurs during the larval stage so it is important to monitor and quantify it. This metadata describes data from laboratory and field studies conducted from 2009 to 2012 for NPRB project 926. The project completed the development of an assay using flow cytometric cell cycle analysis to assess the physiological condition of walleye pollock larvae, and applied it to field collected specimens. The use of RNA to improve the accuracy of the assay was also investigated, as well as the relationship between flow cytometric measurements and growth. Results showed that the overall classification accuracy of the assay (healthy or unhealthy) ranged between 75 and 83% depending on the type of cross-validation testing. A nuclear RNA variable (the ratio of the number of S phase nuclei to the number of high nuclear RNA content G1 phase nuclei) improved overall classification accuracy by 11% compared to a model without it. Unhealthy walleye pollock larvae were present in the southeastern Bering Sea in 2009, and this was most likely the result of cold environmental conditions affecting prey availability and larval feeding. In 2010, no unhealthy larvae were detected. This may be the result of less spatial coverage and smaller collections of larvae than in 2009, given that the environmental conditions were similar between years. |
* Purpose |
To complete the development of an assay to assess the physiological condition of walleye pollock larvae using flow cytometric cell cycle analysis, and to apply it to field collected . |
Notes |
Loaded by batch 3300, 07-23-2012 13:28 |
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Keywords
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ISO 19115 Topic Category | biota |
PARR Exclusion | Non-Federal Funding |
None | flow cytometry |
None | larvae |
None | physiological condition |
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None | Alaska |
None | Bering Sea |
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• » Organization | Alaska Fisheries Science Center |
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• » City | Seattle |
• » State/Province | WA |
• Country | USA |
• » Location Description |
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* Data Set Scope Code | Data Set |
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• Data Set Type | CSV Files |
• Maintenance Frequency | None Planned |
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» Data Presentation Form | Table (digital) |
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* » Support Role | Data Steward |
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* » Date Effective From | 2015 |
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Person | Porter, Steve |
Email Address | steve.porter@noaa.gov |
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* » Support Role | Distributor |
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* » Date Effective From | 2015 |
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Person | Porter, Steve |
Email Address | steve.porter@noaa.gov |
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* » Support Role | Metadata Contact |
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* » Date Effective From | 2019 |
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Person | Bahl, Kimberly |
Email Address | kimberly.bahl@noaa.gov |
Phone | 206 526 4314 |
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* » Date Effective From | 2019 |
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Person | Bahl, Kimberly |
Email Address | kimberly.bahl@noaa.gov |
Phone | 206 526 4314 |
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Currentness Reference | Ground Condition |
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* » W° Bound | -171.1 |
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* » E° Bound | -163.3 |
* » N° Bound | 57.4 |
* » S° Bound | 54.3 |
* » Description |
Bering Sea |
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* » Time Frame Type | Range |
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* » Start | 2009 |
End | 2012 |
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• Data Use Constraints |
User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. |
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» Download URL | https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/RPP/NPRB%20926%3B%20Results%20from%202009%20laboratory%20experiment%20measuring%20nuclear%20RNA.csv |
Distributor | Steve Porter (2015 - Present) |
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Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/14/2021. Contact: Daniel.woodrich@noaa.gov |
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PORTER, S. M., and K. M. BAILEY. 2013. Using measurements of muscle cell nuclear RNA with flow cytometry to improve assessment of larval condition of walleye pollock (Gadus chalcogrammus). Fish. Bull., U.S. 111:337-351. |
Data Management
» Have Resources for Management of these Data Been Identified? | No |
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» Approximate Percentage of Budget for these Data Devoted to Data Management | Unknown |
» Do these Data Comply with the Data Access Directive? | No |
» Is Access to the Data Limited Based on an Approved Waiver? | No |
» If Distributor (Data Hosting Service) is Needed, Please Indicate | |
» Approximate Delay Between Data Collection and Dissemination | varies |
» If Delay is Longer than Latency of Automated Processing, Indicate Under What Authority Data Access is Delayed |
Requires human identification and analysis of samples. |
» Actual or Planned Long-Term Data Archive Location | To Be Determined |
» If To Be Determined, Unable to Archive, or No Archiving Intended, Explain |
No current archive supports this data type. |
» Approximate Delay Between Data Collection and Archiving | varies |
» How Will the Data Be Protected from Accidental or Malicious Modification or Deletion Prior to Receipt by the Archive? |
local and offsite backups |
Lineage
» Lineage Statement |
PORTER, S. M., and K. M. BAILEY. 2013. Using measurements of muscle cell nuclear RNA with flow cytometry to improve assessment of larval condition of walleye pollock (Gadus chalcogrammus). Fish. Bull., U.S. 111:337-351. |
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Catalog Details
Catalog Item ID | 12673 |
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Metadata Record Created By | Nancy Roberson |
Metadata Record Created | 2012-07-23 13:28+0000 |
Metadata Record Last Modified By | SysAdmin InPortAdmin |
» Metadata Record Last Modified | 2023-10-17 16:12+0000 |
Metadata Record Published | 2016-05-18 |
Owner Org | AFSC |
Metadata Publication Status | Published Externally |
Do Not Publish? | N |
Metadata Workflow State | Published / External |
Metadata Last Review Date | 2016-05-18 |
Metadata Review Frequency | 1 Year |
Metadata Next Review Date | 2017-05-18 |
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