17341 AFSC/NMML: North Pacific Killer whale genetic dataset, 1990-2010 Data Set Published / External 23107 Cetacean Assessment and Ecology Program Project In Work The difficulties associated with detecting population boundaries have long constrained the conservation and management of highly mobile marine species, especially for wide-ranging cetaceans such as killer whales (Orcinus orca). In this study, we use molecular genetic data to test a priori hypotheses about population subdivisions generated from a decade of killer whale surveys across the northern North Pacific. A total of 462 skin biopsies were collected from free-swimming killer whales from 1990 to 2010 between the northern Gulf of Alaska in the east and the Sea of Okhotsk in the west, representing both the piscivorous resident and the mammal-eating Biggs (or transient) killer whales. Geographic patterns of genetic differentiation were supported by significant regions of genetic discontinuity providing evidence of population structuring within both lineages, and corroborating direct observations of restricted movements of individual whales. In the Aleutian Islands (Alaska), population strata were largely delimited by major oceanographic boundaries for resident killer whales. In contrast, subdivisions among Biggs killer whales indicated multiple genetic clusters in the Eastern Aleutians and Bering Sea. The presence of sympatric genetic clusters within Biggs whales suggests the presence of isolating mechanisms other than geographic distance within this highly mobile top predator. These data were collected to assess and quantify the population genetic structure of killer whales in the northern North Pacific between the Gulf of Alaska and the Russian Far East. Loaded by FGDC Metadata Uploader, batch 8487, 04-06-2016 13:42 The following FGDC sections are not currently supported in InPort, but were preserved and will be included in the FGDC export: - Taxonomy (FGDC:taxonomy) Theme ISO 19115 Topic Category biota Theme ISO 19115 Topic Category oceans Theme biopsy locations Theme ecotype Theme genetics Theme haplotype Theme mtDNA control region Theme population genetic strata Spatial Aleutian Islands Spatial Bering Sea Spatial Gulf of Alaska Spatial northern North Pacific Alaska Fisheries Science Center Seattle WA USA Data Set As Needed Table (digital) The user is responsible for the results of any application of this data for other than its intended purpose. NOAA denies liability if the data are misused. Contributions to the dataset were also made by: Southwest Fisheries Science Center; North Gulf Oceanic Society; Far East Russia Orca project. NA NA NA 32098 Killer whale genotypes Published / External In Work NA Loaded by FGDC Metadata Uploader, batch 8487, 04-06-2016 13:42 NA YEAR NUMBER No No Active year of sample collection 1990 - 2010 MONTH NUMBER No No Active month of sample collection 4 - 11 SAMPLE ID Unknown No No Active unique sample identifier unknown LAB ID Unknown No No Active genetics lab sample identifier unknown SOURCE Unknown No No Active sample source unknown LAB SEX Unknown No No Active genetically determined sex unknown HAPLOTYPE CODE NUMBER No No Active mtDNA control region haplotype code 0 - 71 EVA1 Unknown No No Active microsatellite locus unknown Fcb11 Unknown No No Active microsatellite locus unknown FCB12 Unknown No No Active microsatellite locus unknown Fcb17 Unknown No No Active microsatellite locus unknown GT48 Unknown No No Active microsatellite locus unknown GT39 Unknown No No Active microsatellite locus unknown Ttr04 Unknown No No Active microsatellite locus unknown Ttr48 Unknown No No Active microsatellite locus unknown Ttr11 Unknown No No Active microsatellite locus unknown BA417 Unknown No No Active microsatellite locus unknown EV37 Unknown No No Active microsatellite locus unknown KW199 Unknown No No Active microsatellite locus unknown Eva5 Unknown No No Active microsatellite locus unknown MK5 Unknown No No Active microsatellite locus unknown KW4 Unknown No No Active microsatellite locus unknown KWM12a Unknown No No Active microsatellite locus unknown GT142 Unknown No No Active microsatellite locus unknown KW207 Unknown No No Active microsatellite locus unknown Dde72 Unknown No No Active microsatellite locus unknown 464 Unknown No No Active microsatellite locus unknown Ttr34 Unknown No No Active microsatellite locus unknown Gata53 Unknown No No Active microsatellite locus unknown Dde66 Unknown No No Active microsatellite locus unknown 415 Unknown No No Active microsatellite locus unknown MK9 Unknown No No Active microsatellite locus unknown Dde70 Unknown No No Active microsatellite locus unknown FCB5 Unknown No No Active microsatellite locus unknown 32099 MTDNA HAPLOTYPES Published / External In Work NA Loaded by FGDC Metadata Uploader, batch 8487, 04-06-2016 13:42 NA ID NUMBER No No Active NA 1 - 15 HAPLOTYPE Unknown No No Active mtDNA control region haplotype common name unknown Haplotype Code NUMBER No No Active mtDNA control region haplotype code 16 - 71 GENBANK ACCESSION NO Unknown No No Active NCBI Genbank accession number unknown ECOTYPE Unknown No No Active killer whale ecotype unknown CONTROL REGION SEQUENCE Unknown No No Active control region genetic sequence unknown Data Steward 2001 Person Parsons, Kim kim.parsons@noaa.gov Distributor 2016 Organization NOAA National Centers for Environmental Information NCEI ncei.info@noaa.gov https://ror.org/04r0wrp59 https://www.ncei.noaa.gov/contact NCEI Contact Information Information for contacts at NCEI. Online Resource Originator 2016-04-06 Organization Alaska Fisheries Science Center AFSC afsc.webmaster@noaa.gov https://ror.org/01h7fye62 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA (206) 526-4000 (206) 526-4004 https://www.fisheries.noaa.gov/about/alaska-fisheries-science-center AKFSC Home Page Online Resource 0700-1700 Pacific Time Point of Contact 2016-04-06 Person Clapham, Phillip phillip.clapham@noaa.gov Ground Condition 144 -141 65 46 North Pacific waters between the Gulf of Alaska and the Sea of Okhotsk. Range 1990 2010 Unclassified Data is available at http://data.nodc.noaa.gov/cgi-bin/iso?id=gov.noaa.nodc:0142202. There are no legal restrictions on access to the data. They reside in public domain and can be freely distributed. User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. https://data.nodc.noaa.gov/cgi-bin/iso?id=gov.noaa.nodc:0142202 Access Not applicable. Not applicable. Genotyping quality control measures included negative control reactions at each step including DNA extraction, PCR, and sequencing, as well as replicate genotyping of multiple samples. An overall genotyping replication rate of =11% of samples allowed us to empirically estimate the per allele genotyping error rate (Hoffman and Amos 2005; Morin et al. 2010). Furthermore, each PCR set included at least two samples previously genotyped to provide cross-plate controls and ensure consistent allele binning throughout the study. No 0 Yes No Unknown Unknown NCEI-MD Unknown IT Security and Contingency Plan for the system establishes procedures and applies to the functions, operations, and resources necessary to recover and restore data as hosted in the Western Regional Support Center in Seattle, Washington, following a disruption. 1 NA 32098 Entity 32099 Entity gov.noaa.nmfs.inport:17341 SysAdmin InPortAdmin 2012-12-21T17:06:52 SysAdmin InPortAdmin 2026-03-04T19:17:18 2018-11-27 Alaska Fisheries Science Center AFSC 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA https://www.afsc.noaa.gov 0700-1700 Pacific Time 1001 Public No No 2018-11-27 1 Year 2019-11-27 Kingdom Animalia Genus Orcinus Species orca Killer whale Killer whale genotypes NA AFSC YEAR year of sample collection AFSC 1990 2010 MONTH month of sample collection AFSC 4 11 SAMPLE ID unique sample identifier AFSC unknown LAB ID genetics lab sample identifier AFSC unknown SOURCE sample source AFSC unknown LAB SEX genetically determined sex AFSC unknown HAPLOTYPE CODE mtDNA control region haplotype code AFSC 0 71 EVA1 microsatellite locus AFSC unknown Fcb11 microsatellite locus AFSC unknown FCB12 microsatellite locus AFSC unknown Fcb17 microsatellite locus AFSC unknown GT48 microsatellite locus AFSC unknown GT39 microsatellite locus AFSC unknown Ttr04 microsatellite locus AFSC unknown Ttr48 microsatellite locus AFSC unknown Ttr11 microsatellite locus AFSC unknown BA417 microsatellite locus AFSC unknown EV37 microsatellite locus AFSC unknown KW199 microsatellite locus AFSC unknown Eva5 microsatellite locus AFSC unknown MK5 microsatellite locus AFSC unknown KW4 microsatellite locus AFSC unknown KWM12a microsatellite locus AFSC unknown GT142 microsatellite locus AFSC unknown KW207 microsatellite locus AFSC unknown Dde72 microsatellite locus AFSC unknown 464 microsatellite locus AFSC unknown Ttr34 microsatellite locus AFSC unknown Gata53 microsatellite locus AFSC unknown Dde66 microsatellite locus AFSC unknown 415 microsatellite locus AFSC unknown MK9 microsatellite locus AFSC unknown Dde70 microsatellite locus AFSC unknown FCB5 microsatellite locus AFSC unknown MTDNA HAPLOTYPES NA AFSC ID NA AFSC 1 15 HAPLOTYPE mtDNA control region haplotype common name AFSC unknown Haplotype Code mtDNA control region haplotype code AFSC 16 71 GENBANK ACCESSION NO NCBI Genbank accession number AFSC unknown ECOTYPE killer whale ecotype AFSC unknown CONTROL REGION SEQUENCE control region genetic sequence AFSC unknown