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Short Citation:
Northwest Fisheries Science Center, 2022: Sex control experiments - Development of methods to sterilize sablefish, https://www.fisheries.noaa.gov/inport/item/17986.

Item Identification

Title: Sex control experiments - Development of methods to sterilize sablefish
Short Name: Sex control experiments - Sablefish Gonadal Differentiation
Status: In Work
Creation Date: 2010-08-16
Publication Date: 2017-07-19
Abstract:

Methods for reproductive sterilization are broadly needed in marine aquaculture due to biosafety concerns associated with escapement of farmed fish and potential genetic contamination of wild stocks. Current methods for sterilization have been primarily tested in freshwater fishes and are often ineffective, particularly in males. The proposed activity builds upon our recent success in producing the first-ever monosex female stocks of sablefish for aquaculture and seeks to develop eco-friendly, non-GMO methods for reproductive sterilization. We will use sablefish as our model marine species for sterilization and continue to collaborate with University of Maryland faculty with expertise in cutting-edge techniques for sterilization. Development of sustainable marine aquaculture is a high priority at the local (NWFSC), regional (WCR), and national levels (NOAA, DOC, Congress). Our sablefish sex control/sterilization project was also highlighted as a critical priority and outstanding area of research in the NOAA Aquaculture Science Review published 28 Feb 2019. We ultimately aim to transfer a sterilization technology to the US sablefish aquaculture industry and expand our methodology to other important species, with the goal of improving sustainability of marine aquaculture and mitigating impacts on the environment.

In these experiments sablefish sex ratio data are collected using gonadal histology and a genetic sex marker.

Purpose:

Laboratory data

Notes:

Loaded by batch 4273, 01-30-2013 19:30

Supplemental Information:

Subject to Public Access to Research Results (PARR): Yes

Keywords

Theme Keywords

Thesaurus Keyword
UNCONTROLLED
None gonadal sex differentiation
None reproductive biology
None sablefish
None sex control
None sustainable aquaculture

Spatial Keywords

Thesaurus Keyword
UNCONTROLLED
None Manchester Research Station
None Northwest Fisheries Science Center

Instrument Keywords

Thesaurus Keyword
UNCONTROLLED
None Video Camera

Physical Location

Organization: Northwest Fisheries Science Center
City: Seattle
State/Province: WA
Country: USA

Data Set Information

Data Set Scope Code: Data Set
Data Set Type: Spreadsheet
Data Presentation Form: Table (digital)

Support Roles

Data Steward

CC ID: 849080
Date Effective From: 2015-10-01
Date Effective To:
Contact (Person): Luckenbach, Adam
Address: 2725 Montlake Blvd East
Seattle, WA 98112
Email Address: Adam.Luckenbach@noaa.gov
Phone: 206-860-3463
Fax: 206-860-3467

Distributor

CC ID: 849081
Date Effective From: 2015-10-01
Date Effective To:
Contact (Organization): Northwest Fisheries Science Center (NWFSC)
Address: 2725 Montlake Boulevard East
Seattle, WA 98112
USA
Email Address: nmfs.nwfsc.metadata@noaa.gov
Phone: 206-860-3200
URL: NWFSC Home

Metadata Contact

CC ID: 849079
Date Effective From: 2015-10-01
Date Effective To:
Contact (Organization): Northwest Fisheries Science Center (NWFSC)
Address: 2725 Montlake Boulevard East
Seattle, WA 98112
USA
Email Address: nmfs.nwfsc.metadata@noaa.gov
Phone: 206-860-3200
URL: NWFSC Home

Originator

CC ID: 849083
Date Effective From: 2015-10-01
Date Effective To:
Contact (Person): Luckenbach, Adam
Address: 2725 Montlake Blvd East
Seattle, WA 98112
Email Address: Adam.Luckenbach@noaa.gov
Phone: 206-860-3463
Fax: 206-860-3467

Point of Contact

CC ID: 849082
Date Effective From: 2015-10-01
Date Effective To:
Contact (Person): Luckenbach, Adam
Address: 2725 Montlake Blvd East
Seattle, WA 98112
Email Address: Adam.Luckenbach@noaa.gov
Phone: 206-860-3463
Fax: 206-860-3467

Extents

Extent Group 1

Extent Group 1 / Geographic Area 1

CC ID: 849089
W° Bound: -122.3062
E° Bound: -122.3062
N° Bound: 47.6449
S° Bound: 47.6449
Description

Northwest Fisheries Science Center: Montlake lab

Extent Group 1 / Time Frame 1

CC ID: 849088
Time Frame Type: Continuing
Start: 2010-05-01

Extent Group 2

Extent Group 2 / Geographic Area 1

CC ID: 849092
W° Bound: -122.5547
E° Bound: -122.5547
N° Bound: 47.569
S° Bound: 47.569
Description

Manchester Research Station: Manchester lab

Extent Group 2 / Time Frame 1

CC ID: 849091
Time Frame Type: Continuing
Start: 2010-05-01

Access Information

Security Class: Unclassified
Data Access Procedure:

At this time, contact the Data Manager for information on obtaining access to this data set. In the near future, the NWFSC will strive to provide all data resources as a web service in order to meet the NOAA Data Access Policy Directive (https://nosc.noaa.gov/EDMC/PD.DA.php).

Data Access Constraints:

NA

Distribution Information

Distribution 1

CC ID: 909684
Start Date: 2020-06-02
End Date: Present
Download URL: https://www.webapps.nwfsc.noaa.gov/apex/parr/sablefish_sex_differentiation_sample_data/data/page/
Distributor: Northwest Fisheries Science Center (NWFSC) (2015-10-01 - Present)
File Name: Sablefish Sex Differentiation Sample Data (RESTful)
Description:

This table contains fish metrics and gonadal gene expression data for juvenile sablefish sampled during the process of sex differentiation.

Distribution 2

CC ID: 909685
Start Date: 2020-06-02
End Date: Present
Download URL: https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/sablefish_sex_differentiation_sample_data
Distributor: Northwest Fisheries Science Center (NWFSC) (2015-10-01 - Present)
File Name: Sablefish Sex Differentiation Sample Data
Description:

This table contains fish metrics and gonadal gene expression data for juvenile sablefish sampled during the process of sex differentiation.

Distribution 3

CC ID: 849085
Start Date: 2017-07-19
End Date: Present
Download URL: https://www.webapps.nwfsc.noaa.gov/apex/parr/sablefish_sex_differentiation_sampling/data/page/
Distributor: Northwest Fisheries Science Center (NWFSC) (2015-10-01 - Present)
File Name: Sablefish sex differentiation sampling (RESTful)
Description:

This table contains morphometric and histological data from cultured sablefish sampled for assessment of normal sex differentiation.

Distribution 4

CC ID: 849084
Start Date: 2017-07-19
End Date: Present
Download URL: https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/sablefish_sex_differentiation_sampling
Distributor: Northwest Fisheries Science Center (NWFSC) (2015-10-01 - Present)
File Name: Sablefish sex differentiation sampling
Description:

This table contains morphometric and histological data from cultured sablefish sampled for assessment of normal sex differentiation.

URLs

URL 1

CC ID: 849086
URL: https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/10192
Name: Sex control experiments
URL Type:
Online Resource
File Resource Format: Web site
Description:

NWFSC Dataset Information page. In these experiments sablefish sex ratio data are collected using gonadal histology and a genetic sex marker.

Technical Environment

Description:

Spreadsheet

Data Quality

Accuracy:

Medium

Quality Control Procedures Employed:

Slides and gel images viewed blind to treatment to avoid bias.

Data Management

Have Resources for Management of these Data Been Identified?: No
Approximate Percentage of Budget for these Data Devoted to Data Management: 1
Do these Data Comply with the Data Access Directive?: Yes
Is Access to the Data Limited Based on an Approved Waiver?: No
If Distributor (Data Hosting Service) is Needed, Please Indicate: Yes
Approximate Delay Between Data Collection and Dissemination: 0 days
If Delay is Longer than Latency of Automated Processing, Indicate Under What Authority Data Access is Delayed:

No Delay

Actual or Planned Long-Term Data Archive Location: NCEI-MD
Approximate Delay Between Data Collection and Archiving: 365
How Will the Data Be Protected from Accidental or Malicious Modification or Deletion Prior to Receipt by the Archive?:

The Northwest Fisheries Science Center facilitates backup and recovery of all data and IT components which are managed by IT Operations through the capture of static (point-in-time) backup data to physical media. Once data is captured to physical media (every 1-3 days), a duplicate is made and routinely (weekly) transported to an offsite archive facility where it is maintained throughout the data's applicable life-cycle.

Lineage

Lineage Statement:

Tissue sections and genetic marker results analyzed by researchers and notes typically recorded in spreadsheet on sex and stage of gonads.

Child Items

Rubric scores updated every 15m

Rubric Score Type Title
Entity Sablefish Sex Differentiation Sample Data
Entity Sablefish sex differentiation sampling

Catalog Details

Catalog Item ID: 17986
GUID: gov.noaa.nmfs.inport:17986
Metadata Record Created By: Jeffrey W Cowen
Metadata Record Created: 2013-01-30 19:30+0000
Metadata Record Last Modified By: Jeffrey W Cowen
Metadata Record Last Modified: 2020-06-02 19:02+0000
Metadata Record Published: 2019-06-04
Owner Org: NWFSC
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Next Review Date: 2020-06-04