17986 Sex control experiments - Sablefish Gonadal Differentiation Data Set Published / External 17938 Migrated from Resource Enhancement and Utilization Technologies Division Project In Work 2010-08-16 2017-07-19 Methods for reproductive sterilization are broadly needed in marine aquaculture due to biosafety concerns associated with escapement of farmed fish and potential genetic contamination of wild stocks. Current methods for sterilization have been primarily tested in freshwater fishes and are often ineffective, particularly in males. The proposed activity builds upon our recent success in producing the first-ever monosex female stocks of sablefish for aquaculture and seeks to develop eco-friendly, non-GMO methods for reproductive sterilization. We will use sablefish as our model marine species for sterilization and continue to collaborate with University of Maryland faculty with expertise in cutting-edge techniques for sterilization. Development of sustainable marine aquaculture is a high priority at the local (NWFSC), regional (WCR), and national levels (NOAA, DOC, Congress). Our sablefish sex control/sterilization project was also highlighted as a critical priority and outstanding area of research in the NOAA Aquaculture Science Review published 28 Feb 2019. We ultimately aim to transfer a sterilization technology to the US sablefish aquaculture industry and expand our methodology to other important species, with the goal of improving sustainability of marine aquaculture and mitigating impacts on the environment. In these experiments sablefish sex ratio data are collected using gonadal histology and a genetic sex marker. Laboratory data Loaded by batch 4273, 01-30-2013 19:30 Subject to Public Access to Research Results (PARR): Yes Theme gonadal sex differentiation Theme reproductive biology Theme sablefish Theme sex control Theme sustainable aquaculture Spatial Manchester Research Station Spatial Northwest Fisheries Science Center Instrument Video Camera Northwest Fisheries Science Center Seattle WA USA Data Set Spreadsheet Table (digital) Video Camera Platform Not Applicable Not Applicable 46800 Sablefish sex differentiation sampling Published / External Planned This table contains morphometric and histological data from cultured sablefish sampled for assessment of normal sex differentiation. Spreadsheet Yes PARR This table contains morphometric and histological data from cultured sablefish sampled for assessment of normal sex differentiation. 1 Sampling date date No No Active Date when sample was collected. Units for values are month-day-year. date month-day-year 2 Origin, Dissection text No No Active Fish origin and dissection type used. text 3 Fish # text No No Active Number assigned to sample collected. text 4 Body Wt (g) number No No Active Fish body weight of the sample. Units for values are grams. number grams 5 FL (mm) number No No Active Fish fork length of the sample. Units for values are millimeters. number millimeters 6 Gonad Wt (g) number No No Active Total weight of gonads of the sample. Units for values are grams. number grams 7 Genetic sex text No No Active Fish genetic sex: male (M) or female (F). text 8 Histo sex text No No Active Sex of the fish as determined by histology. text 9 Histology notes text No No Active Notes taken while analyzing histology. text 10 Sampling notes text No No Active Notes taken while sampling fish. text 59918 Sablefish Sex Differentiation Sample Data Published / External Planned This table contains fish metrics and gonadal gene expression data for juvenile sablefish sampled during the process of sex differentiation. Spreadsheet Yes PARR This table contains fish metrics and gonadal gene expression data for juvenile sablefish sampled during the process of sex differentiation. 1 Sample number number No No Active Identifier for sample. Units for values are NA. number NA 2 Sampling date text No No Active Date fish sampled. Units for values are month-day-year. text month-day-year 3 Cohort year number No No Active Year sampled fish were spawned. Units for values are NA. number NA 4 Genotypic sex text No No Active Genotypic sex determined for the fish: M, male; F, female. Units for values are NA. text NA 5 Bin Length number No No Active Fork length bins used for graphing. Units for values are NA. number NA 6 Fork length number No No Active Fish fork length recorded at sampling. Units for values are millimeters. number millimeters 7 Body weight number No No Active Fish body weight recorded at sampling. Units for values are grams. number grams 8 Tissue text No No Active Sampling tissue method:trunk or gonads only. Units for values are NA. text NA 9 eef1a_Ct number No No Active Candidate reference gene expression qPCR cycle threshold value. Units for values are NA. number NA 10 eef1a_RQ number No No Active Candidate reference gene expression qPCR relative quantity. Units for values are NA. number NA 11 actb_Ct number No No Active Candidate reference gene expression qPCR cycle threshold value. Units for values are NA. number NA 12 actb_RQ number No No Active Candidate reference gene expression qPCR relative quantity. Units for values are NA. number NA 13 btf3_Ct number No No Active Candidate reference gene expression qPCR cycle threshold value. Units for values are NA. number NA 14 btf3_RQ number No No Active Candidate reference gene expression qPCR relative quantity. Units for values are NA. number NA 15 rpl4_Ct number No No Active Candidate reference gene expression qPCR cycle threshold value. Units for values are NA. number NA 16 rpl4_RQ number No No Active Candidate reference gene expression qPCR relative quantity. Units for values are NA. number NA 17 cytb_Ct number No No Active Candidate reference gene expression qPCR cycle threshold value. Units for values are NA. number NA 18 cytb_RQ number No No Active Candidate reference gene expression qPCR relative quantity. Units for values are NA. number NA 19 Refrence Gene Geometric Mean number No No Active Geometric mean of the three most stable reference genes (actb, btf3, rpl4) used for gene normalization of target genes (RefGeoMean). Units for values are NA. number NA 20 dmrt1_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 21 dmrt1_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 22 dmrt1_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 23 dmrt1_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 24 cyp19a1a_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 25 cyp19a1a_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 26 cyp19a1a_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 27 cyp19a1a_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 28 amh_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 29 amh_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 30 amh_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 31 amh_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 32 hsd3b1_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 33 hsd3b1_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 34 hsd3b1_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 35 hsd3b1_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 36 cyp11b_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 37 cyp11b_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 38 cyp11b_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 39 cyp11b_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 40 fshr_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 41 fshr_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 42 fshr_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 43 fshr_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 44 foxl2_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 45 foxl2_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 46 foxl2_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 47 foxl2_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 48 nr5a1_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 49 nr5a1_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 50 nr5a1_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 51 nr5a1_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 52 amhrII_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 53 amhrII_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 54 amhrII_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 55 amhrII_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 56 cyp11a1_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 57 cyp11a1_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 58 cyp11a1_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 59 cyp11a1_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 60 gdf9_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 61 gdf9_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 62 gdf9_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 63 gdf9_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 64 sox9a_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 65 sox9a_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 66 sox9a_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 67 sox9a_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 68 star_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 69 star_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 70 star_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 71 star_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 72 inha_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 73 inha_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 74 inha_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 75 inha_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 76 cyp17a2_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 77 cyp17a2_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 78 cyp17a2_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 79 cyp17a2_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 80 gsdf_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 81 gsdf_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 82 gsdf_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 83 gsdf_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 84 nr0b1_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 85 nr0b1_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 86 nr0b1_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 87 nr0b1_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 88 vasa_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 89 vasa_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 90 vasa_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 91 vasa_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 92 zp3_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 93 zp3_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 94 zp3_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 95 zp3_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 96 figla_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 97 figla_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 98 figla_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 99 figla_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 100 igf3_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 101 igf3_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 102 igf3_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 103 igf3_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 104 fst_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 105 fst_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 106 fst_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 107 fst_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 108 esr1_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 109 esr1_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 110 esr1_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 111 esr1_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 112 ar1_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 113 ar1_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 114 ar1_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 115 ar1_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 116 ar2_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 117 ar2_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 118 ar2_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 119 ar2_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 120 pou5f3_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 121 pou5f3_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 122 pou5f3_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 123 pou5f3_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA 124 lhcgr_Ct number No No Active Target gene expression qPCR cycle threshold value. Units for values are NA. number NA 125 lhcgr_RQ number No No Active Target gene expression qPCR relative quantity. Units for values are NA. number NA 126 lhcgr_Ref number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean. Units for values are NA. number NA 127 lhcgr_Norm number No No Active Target gene expression qPCR relative quantity normalized to RefGeoMean and tissue type. Units for values are NA. number NA Data Steward 2015-10-01 Person Luckenbach, Adam Adam.Luckenbach@noaa.gov 2725 Montlake Blvd East Seattle WA 98112 206-860-3463 206-860-3467 Distributor 2015-10-01 Organization Northwest Fisheries Science Center NWFSC nmfs.nwfsc.metadata@noaa.gov https://ror.org/05r7z1k40 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov NWFSC Home Online Resource Metadata Contact 2015-10-01 Organization Northwest Fisheries Science Center NWFSC nmfs.nwfsc.metadata@noaa.gov https://ror.org/05r7z1k40 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov NWFSC Home Online Resource Originator 2015-10-01 Person Luckenbach, Adam Adam.Luckenbach@noaa.gov 2725 Montlake Blvd East Seattle WA 98112 206-860-3463 206-860-3467 Point of Contact 2015-10-01 Person Luckenbach, Adam Adam.Luckenbach@noaa.gov 2725 Montlake Blvd East Seattle WA 98112 206-860-3463 206-860-3467 -122.3062 -122.3062 47.6449 47.6449 Northwest Fisheries Science Center: Montlake lab Continuing 2010-05-01 -122.5547 -122.5547 47.569 47.569 Manchester Research Station: Manchester lab Continuing 2010-05-01 Unclassified At this time, contact the Data Manager for information on obtaining access to this data set. In the near future, the NWFSC will strive to provide all data resources as a web service in order to meet the NOAA Data Access Policy Directive (https://nosc.noaa.gov/EDMC/PD.DA.php). NA https://www.webapps.nwfsc.noaa.gov/apex/parr/sablefish_sex_differentiation_sample_data/data/page/ 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 Sablefish Sex Differentiation Sample Data (RESTful) This table contains fish metrics and gonadal gene expression data for juvenile sablefish sampled during the process of sex differentiation. https://www.webapps.nwfsc.noaa.gov/apex/parr/sablefish_sex_differentiation_sampling/data/page/ 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 Sablefish sex differentiation sampling (RESTful) This table contains morphometric and histological data from cultured sablefish sampled for assessment of normal sex differentiation. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/sablefish_sex_differentiation_sample_data 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 Sablefish Sex Differentiation Sample Data This table contains fish metrics and gonadal gene expression data for juvenile sablefish sampled during the process of sex differentiation. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/sablefish_sex_differentiation_sampling 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 Sablefish sex differentiation sampling This table contains morphometric and histological data from cultured sablefish sampled for assessment of normal sex differentiation. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/10192 Sex control experiments Online Resource Web site NWFSC Dataset Information page. In these experiments sablefish sex ratio data are collected using gonadal histology and a genetic sex marker. Spreadsheet Medium Slides and gel images viewed blind to treatment to avoid bias. No 1 Yes No Yes 0 days No Delay NCEI-MD 365 The Northwest Fisheries Science Center facilitates backup and recovery of all data and IT components which are managed by IT Operations through the capture of static (point-in-time) backup data to physical media. Once data is captured to physical media (every 1-3 days), a duplicate is made and routinely (weekly) transported to an offsite archive facility where it is maintained throughout the data's applicable life-cycle. Tissue sections and genetic marker results analyzed by researchers and notes typically recorded in spreadsheet on sex and stage of gonads. 59918 Entity 46800 Entity gov.noaa.nmfs.inport:17986 Jeffrey Cowen 2013-01-30T19:30:53 SysAdmin InPortAdmin 2026-03-04T19:17:17 2019-06-04 Northwest Fisheries Science Center NWFSC 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov 1001 Public No No 2019-06-04 1 Year 2020-06-04