18568 Oregon ESA 2010 BKD vertical transmission (Test of analyses for bacterial kidney disease as predictors of vertical transmission) Data Set Published / External 17938 Migrated from Resource Enhancement and Utilization Technologies Division Project In Work 2013-05-31 Although the pathogen causing bacterial kidney disease is known to be transmitted from broodstock female to offspring, there is large uncertainty around the likelihood of vertical transmission. Identifying predictors of transmission by analyzing broodstock tissues for pathogen presence would provide an important management tool for hatcheries and captive broodstock programs. This project assesses a suite of analytical methods for predictive utility by evaluating broodstock and following the infection fate of their offspring. In addition to measuring fork length (mm) & weight (g) of individual offspring, kidney tissue is analyzed for Renibacterium salmoninarum by enzyme-linked immunoassay (ELISA), fluorescent antibody microscopy, and duplex quantitative real-time polymerase chain reaction (qPCR). Individual fish data are recorded by spreadsheet. This project is conducted by Oregon Department of Fish & Wildlife in collaboration with NOAA personnel. This is a self-contained project. BKD vertical transmission lab analyses results. Laboratory data Loaded by batch 4462, 02-26-2013 13:23 These data are available to the public Report: Test of analyses for bacterial kidney disease as predictors of vertical transmission (agency report of study) Peer Reviewed Publication: Risk assessment of vertical transmission of Renibacterium salmoninarum (publication describing results) Theme PARR Exclusion Non-NOAA Funded Theme Biological Theme artificial propagation Theme chinook salmon Theme pathogen Spatial Eastern Oregon University, La Grande, OR Northwest Fisheries Science Center Seattle WA USA Data Set Table (digital) Quantitative PCR Machine Platform Not Applicable Animal and Plant Collection Device Data Steward 2015-10-01 Person Rhodes, Linda D Linda.Rhodes@noaa.gov 2725 Montlake Blvd East Seattle WA 98112 206-860-3279 206-860-3467 Distributor 2015-10-01 Organization Northwest Fisheries Science Center NWFSC nmfs.nwfsc.metadata@noaa.gov https://ror.org/05r7z1k40 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov NWFSC Home Online Resource Metadata Contact 2015-10-01 Person Contact, Metadata nmfs.nwfsc.metadata@noaa.gov 2725 Montlake Boulevard East Seattle WA 98112 USA (206) 860-3433 Originator 2015-10-01 Person Rhodes, Linda D Linda.Rhodes@noaa.gov 2725 Montlake Blvd East Seattle WA 98112 206-860-3279 206-860-3467 Point of Contact 2015-10-01 Person Maynard, Desmond Des.Maynard@noaa.gov 7305 East Beach Drive Manchester WA 98366 360-871-8313 -118.0829 -118.0829 45.3157 45.3157 Eastern Oregon University, La Grande, OR: Eastern Oregon University lab, La Grande, OR Continuing 2014-01-01 Unclassified Request submission by email or telephone NA https://www.ncei.noaa.gov http://www.ncei.noaa.gov Spreadsheet Medium Positive & negative controls, no template controls, comparison with previous curves No 5% Yes No No 90 days No Delay No Archiving Intended 365 days The Northwest Fisheries Science Center facilitates backup and recovery of all data and IT components which are managed by IT Operations through the capture of static (point-in-time) backup data to physical media. Once data is captured to physical media (every 1-3 days), a duplicate is made and routinely (weekly) transported to an offsite archive facility where it is maintained throughout the data's applicable life-cycle. Standard curve used to determine copy number NWFSC Annual Project Planning System https://www.webapps.nwfsc.noaa.gov/5703 gov.noaa.nmfs.inport:18568 SysAdmin InPortAdmin 2013-02-26T13:23:11 SysAdmin InPortAdmin 2026-03-04T19:17:17 2016-02-29 Northwest Fisheries Science Center NWFSC 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov 1001 Public No No 2016-02-29 1 Year 2017-02-28