20598 PNW cetacean muscle biochemistry Data Set Published / External 20585 CB Legacy Data Sets Project Completed 2013-08-04 2016-04-13 This project assesses the development of two important skeletal muscle adaptations for diving (enhanced myoglobin content and acid buffering capacities) in a range of cetaceans from the Pacific Northwest in order to understand constraints on diving capacities (primarily dive duration) with development. Myoglobin is an important oxygen store for supporting aerobic diving, while enhanced buffering capacity could support anaerobic metabolism during apnea. Yet little is known about the post-natal development of muscle biochemistry in cetaceans. To assess the development of these important adaptations in cetaceans, muscle myoglobin content and buffering capacity due to non-bicarbonate buffers were measured in the longissimus dorsi of harbor porpoise (Phocoena phocoena), killer whale (Orcinus orca), and Dall's porpoise (Phocoenoides dalli). Dawn Noren is the principal investigator from NWFSC. Projects on individual species within this larger project include different collaborators. For the project on harbor porpoise (completed), Shawn Noren (UCSC) and Joe Gaydos (SeaDoc Society) are external collaborators. Dawn Noren is responsible for project oversight, Shawn Noren trained Northwest Fisheries Science Center (NWFSC) staff on laboratory procedures for sample analysis and Joe Gaydos collected samples. The specific work includes experimental design, data collection, data analysis, and writing for submission to peer-reviewed journals. These projects are ongoing until sufficient samples are collected for sound statistical analysis. This research addresses some data gaps associated with dive capacities and potential limitations in habitat use by local cetaceans. Muscle adaptation to diving. Raw/field data, Laboratory data Loaded by FGDC Metadata Uploader, batch 4724, 09-09-2013 14:33 Subject to Public Access to Research Results (PARR): Yes Peer Reviewed Publication: Living in the fast lane: rapid development of the locomotor muscle in immature harbor porpoises (Phocoena phocoena). Theme Dall's porpoise Theme acid buffering Theme diving physiology Theme harbor porpoise Theme killer whale Theme myoglobin Spatial NWFSC Montlake Instrument 92372 Platform 92606 Northwest Fisheries Science Center Seattle WA USA Data Set Spreadsheet Table (digital) 92372 Platform Not Applicable 92606 33385 WA Harbor Porpoise Myoglobin Data Published / External Planned Washington Harbor Porpoise Myoglobin. Spreadsheet Yes PARR Washington Harbor Porpoise Myoglobin. 1 Animal VARCHAR2 No No Active Necropsy Identificaiton Number. VARCHAR2 2 Age-Class VARCHAR2 No No Active Generalized Lifestage. VARCHAR2 calf, neonate, adult, sub-adult, yearling, fetus 3 Sex VARCHAR2 No No Active Gender of animal. VARCHAR2 M, F 4 Length NUMBER No No Active Body length from tip of rostrum to tail notch in cm. Units for values are cm. NUMBER cm 5 Trial 1 MB NUMBER No No Active Outcome of first laboratory trial to determine myoglobin content of swimming muscle. Units for values are (g/(100g wet muscle)). NUMBER (g/(100g wet muscle)) 6 Trial 2 MB NUMBER No No Active Outcome of second laboratory trial to determine myoglobin content of swimming muscle. Units for values are (g/(100g wet muscle)). NUMBER (g/(100g wet muscle)) 7 Trial 3 MB NUMBER No No Active Outcome of third laboratory trial to determine myoglobin content of swimming muscle. Units for values are (g/(100g wet muscle)). NUMBER (g/(100g wet muscle)) 8 Trial 4 MB NUMBER No No Active Outcome of fourth laboratory trial to determine myoglobin content of swimming muscle. Units for values are (g/(100g wet muscle)). NUMBER (g/(100g wet muscle)) 9 Trial 5 MB NUMBER No No Active Outcome of fifth laboratory trial to determine myoglobin content of swimming muscle. Units for values are (g/(100g wet muscle)). NUMBER (g/(100g wet muscle)) 10 MEAN AB NUMBER No No Active Arithmetic mean of usable trial results. Units for values are (g/(100g wet muscle)). NUMBER (g/(100g wet muscle)) 11 MB STD DEV NUMBER No No Active Standard deviation for usable trial results. NUMBER 12 COMMENTS VARCHAR2 No No Active Lab notes and notes on the condition of the carcass. VARCHAR2 33386 WA Harbor Porpoise Acid Buffer Data Published / External Planned Washington Harbor Porpoise Acid Buffer. Spreadsheet Yes PARR Washington Harbor Porpoise Acid Buffer. 1 Animal VARCHAR2 No No Active Necropsy Identificaiton Number. VARCHAR2 2 Age-Class VARCHAR2 No No Active Generalized Lifestage. VARCHAR2 calf, neonate, adult, sub-adult, yearling, fetus 3 Sex VARCHAR2 No No Active Gender of animal. VARCHAR2 M, F 4 Length NUMBER No No Active Body length from tip of rostrum to tail notch in cm. Units for values are cm. NUMBER cm 5 Trial 1 AB NUMBER No No Active Outcome of first laboratory trial to determine acid buffering of swimming muscle. Units for values are slykes. NUMBER slykes 6 Trial 2 AB NUMBER No No Active Outcome of second laboratory trial to determine acid buffering of swimming muscle. Units for values are slykes. NUMBER slykes 7 Trial 3 AB NUMBER No No Active Outcome of third laboratory trial to determine acid buffering of swimming muscle. Units for values are slykes. NUMBER slykes 8 MEAN AB NUMBER No No Active Arithmetic mean of usable trial results. Units for values are slykes. NUMBER slykes 9 AB STD DEV NUMBER No No Active Standard deviation for usable trial results. NUMBER 10 COMMENTS VARCHAR2 No No Active Lab notes and notes on the condition of the carcass. VARCHAR2 Data Steward 2015-10-01 Person Noren Adams, Dawn Dawn.Noren@noaa.gov 2725 Montlake Boulevard East Seatle WA 98112 206-302-2439 Distributor 2015-10-01 Organization Northwest Fisheries Science Center NWFSC nmfs.nwfsc.metadata@noaa.gov https://ror.org/05r7z1k40 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov NWFSC Home Online Resource Metadata Contact 2015-10-01 Organization Northwest Fisheries Science Center NWFSC nmfs.nwfsc.metadata@noaa.gov https://ror.org/05r7z1k40 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov NWFSC Home Online Resource Originator 2015-10-01 Person Noren Adams, Dawn Dawn.Noren@noaa.gov 2725 Montlake Boulevard East Seatle WA 98112 206-302-2439 Point of Contact 2015-10-01 Person Noren Adams, Dawn Dawn.Noren@noaa.gov 2725 Montlake Boulevard East Seatle WA 98112 206-302-2439 -122.3062 -122.3062 47.6449 47.6449 NWFSC Montlake: Northwest Fisheries Science Center (NWFSC) in Seattle, WA USA Continuing 2004-01-01 Unclassified At this time, contact the Data Manager for information on obtaining access to this data set. In the near future, the NWFSC will strive to provide all data resources as a web service in order to meet the NOAA Data Access Policy Directive (https://nosc.noaa.gov/EDMC/PD.DA.php). NA https://www.webapps.nwfsc.noaa.gov/apex/parr/wa_harbor_porpoise_acid_buffer_data/data/page/ 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 WA Harbor Porpoise Acid Buffer Data (RESTful) Washington Harbor Porpoise Acid Buffer. https://www.webapps.nwfsc.noaa.gov/apex/parr/wa_harbor_porpoise_myoglobin_data/data/page/ 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 WA Harbor Porpoise Myoglobin Data (RESTful) Washington Harbor Porpoise Myoglobin. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/wa_harbor_porpoise_acid_buffer_data 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 WA Harbor Porpoise Acid Buffer Data Washington Harbor Porpoise Acid Buffer. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/wa_harbor_porpoise_myoglobin_data 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 WA Harbor Porpoise Myoglobin Data Washington Harbor Porpoise Myoglobin. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/10852 PNW cetacean muscle biochemistry Online Resource Web site NWFSC Dataset Information page. Muscle adaptation to diving Spreadsheet High Samples run in triplicate. These data were collected and processed in accordance with established protocols and best practices under the direction of the project’s Principal Investigator. Contact the dataset Data Manager for full QA/QC methodology. No 10 No No No 0 days No Delay NCEI-MD 365 The Northwest Fisheries Science Center facilitates backup and recovery of all data and IT components which are managed by IT Operations through the capture of static (point-in-time) backup data to physical media. Once data is captured to physical media (every 1-3 days), a duplicate is made and routinely (weekly) transported to an offsite archive facility where it is maintained throughout the data's applicable life-cycle. Biochemical analysis of muscle. 33386 Entity 33385 Entity gov.noaa.nmfs.inport:20598 Robert Marsicek 2013-09-09T14:33:46 SysAdmin InPortAdmin 2026-03-04T19:17:17 2019-06-04 Northwest Fisheries Science Center NWFSC 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov 1001 Public No No 2019-06-04 1 Year 2020-06-04