23101 AFSC/REFM: Pacific cod genetics in the Aleutian Islands 2004-2007 Data Set Published / External 23100 Genetics Project Completed 2013-10 Landscape genetics of Pacific cod Gadus macrocephalus within the Bering Sea and Aleutian Islands (BSAI) management area of Alaska was examined in samples from nine spawning locations, including one temporal replicate sample, using 17 microsatellite DNA markers. This study examined fine-scale population structure of Pacific cod in the BSAI with the goal of identifying breaks in genetic continuity associated with physical barriers to migration and larval transport. Samples were taken from spawning fish collected from the western Aleutian Islands east to Unimak Pass and as far north as the Pribilof Islands. Overall, this work confirms previous studies, which found that genetic differentiation between samples is proportional to the distance between them, and also resolves population structure on a scale not previously possible. Results indicate that dispersal is not continuous at a fine scale and several distinct genetic groups were identified that correspond to differences in the physical environment of the BSAI. The data provide evidence for limited connectivity among spawning groups; in particular, there is evidence that a barrier exists between the Aleutian Islands and the eastern Bering Sea. In addition, analysis of molecular variance and Monmonier algorithm results suggest that, within the Bering Sea, the Unimak Pass and Pribilof Islands spawning groups may be distinct from each other, and the Wombling analysis indicates that samples west of Amchitka Pass in the western Aleutians may be distinct from those in the eastern Aleutians. To examine genetic population structure of Pacific cod in the Bering Sea and Aleutian Islands. Loaded by FGDC Metadata Uploader, batch 5325, 02-11-2015 17:28 The following FGDC sections are not currently supported in InPort, but were preserved and will be included in the FGDC export: - Taxonomy (FGDC:taxonomy) - Lineage (FGDC:lineage) Theme ISO 19115 Topic Category biota Theme ISO 19115 Topic Category oceans Theme PARR Exclusion Non-Federal Funding Theme genetics Theme microsatellites Theme population structure Spatial Aleutian Islands Spatial Bering Sea North Pacific Research Board Anchorage AK USA Data Set None Planned Table (digital) Samples were collected by fishery observers and Olav Ormseth. na na na 23102 csv file Published / External Completed Loaded by FGDC Metadata Uploader, batch 5325, 02-11-2015 17:28 specimen NUMBER No No Active Individual fish from sampling locations described in Table 2 of the manuscript. 1 - 998 Gma100 NUMBER No No Active Column names are the names of the microsatellite loci, (e.g. Gma100, etc). There are two columns with the same name because each fish has 2 copies of each allele (one from each parent). 0 - 398 Gma100.1 NUMBER No No Active NA 0 - 424 Gma101 NUMBER No No Active NA 0 - 218 Gma101.1 NUMBER No No Active NA 0 - 334 Gma102 NUMBER No No Active NA 0 - 256 Gma102.1 NUMBER No No Active NA 0 - 268 Gma103 NUMBER No No Active NA 0 - 300 Gma103.1 NUMBER No No Active NA 0 - 356 Gma104 NUMBER No No Active NA 0 - 204 Gma104.1 NUMBER No No Active NA 0 - 244 Gma105 NUMBER No No Active NA 0 - 172 Gma105.1 NUMBER No No Active NA 0 - 182 Gma108 NUMBER No No Active NA 0 - 219 Gma108.1 NUMBER No No Active NA 0 - 287 Gma109 NUMBER No No Active NA 0 - 340 Gma109.1 NUMBER No No Active NA 0 - 360 Gmo37 NUMBER No No Active NA 0 - 280 Gmo37.1 NUMBER No No Active NA 0 - 366 Tch20 NUMBER No No Active NA 0 - 176 Tch20.1 NUMBER No No Active NA 0 - 212 GmoG13 NUMBER No No Active NA 0 - 239 GmoG13.1 NUMBER No No Active NA 0 - 247 GmoG16 NUMBER No No Active NA 0 - 168 GmoG16.1 NUMBER No No Active NA 0 - 176 Gma107 NUMBER No No Active NA 0 - 456 Gma107.1 NUMBER No No Active NA 0 - 456 Gmo19 NUMBER No No Active NA 0 - 214 Gmo19.1 NUMBER No No Active NA 0 - 298 GmoC82 NUMBER No No Active NA 0 - 136 GmoC82.1 NUMBER No No Active NA 0 - 140 GmoC83 NUMBER No No Active NA 0 - 102 GmoC83.1 NUMBER No No Active NA 0 - 106 Tch13 NUMBER No No Active NA 0 - 90 Tch13.1 NUMBER No No Active NA 0 - 98 GmoG5 NUMBER No No Active NA 0 - 196 GmoG5.1 NUMBER No No Active NA 0 - 200 Data Steward 2015-10-19 Person Spies, Ingrid ingrid.spies@noaa.gov Distributor 2015-10-19 Organization North Pacific Research Board NPRB 1007 West 3rd Avenue, Suite 100 Anchorage AK 99501 USA 907 644 6700 http://www.nprb.org/index.html Originator 2015-02-11 Person Spies, Ingrid ingrid.spies@noaa.gov Originator 2015-02-11 Organization Alaska Fisheries Science Center AFSC afsc.webmaster@noaa.gov https://ror.org/01h7fye62 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA (206) 526-4000 (206) 526-4004 https://www.fisheries.noaa.gov/about/alaska-fisheries-science-center AKFSC Home Page Online Resource 0700-1700 Pacific Time Point of Contact 2015-02-11 Person Spies, Ingrid ingrid.spies@noaa.gov Ground Condition 170 -170 55 50 Aleutian Islands Range 2004 2007 Unclassified na There are no legal restrictions on access to the data. They reside in public domain and can be freely distributed. User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. http://projects.nprb.org/#metadata/0be47376-91fb-4021-b1a1-e42a4a112f53/project/files https://www.afsc.noaa.gov/metadata-images/REFM/SSMApcod_al_genetics.png Map Browse Graphic URL Specimen collection locations. https://workspace.aoos.org/published/file/8acc25db-42a4-4818-bca8-f3fb83b442bd/817_Final%20report.pdf?source=catalog&portalId=92 Online Resource PDF Excel Data quality was evaluated using a double-blind genotyping protocol, where two individuals score the gel image independently. This method was employed on all samples run on the ABI platform (12 loci), and multiple runs of the same sample were compared for reliability on samples run with the MegaBACE genotyping platform. All loci were rerun up to four times when scores were questionable to ensure scoring reliability. Opportunistic collections. See accuracy report. See lineage. Yes Unknown Yes No no unknown na Other unknown na View metadata record as uploaded FGDC record. 23102 Entity gov.noaa.nmfs.inport:23101 Angie Greig 2015-02-11T17:26:49 SysAdmin InPortAdmin 2026-03-04T19:17:17 2016-04-28 Alaska Fisheries Science Center AFSC 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA https://www.afsc.noaa.gov 0700-1700 Pacific Time 1001 Public No No 2016-04-28 1 Year 2017-04-28 none Pacific cod Kingdom Animalia Genus Gadus Species macrocephalus Pacific cod csv file AFSC specimen Individual fish from sampling locations described in Table 2 of the manuscript. AFSC 1 998 Gma100 Column names are the names of the microsatellite loci, (e.g. Gma100, etc). There are two columns with the same name because each fish has 2 copies of each allele (one from each parent). AFSC 0 398 Gma100.1 NA AFSC 0 424 Gma101 NA AFSC 0 218 Gma101.1 NA AFSC 0 334 Gma102 NA AFSC 0 256 Gma102.1 NA AFSC 0 268 Gma103 NA AFSC 0 300 Gma103.1 NA AFSC 0 356 Gma104 NA AFSC 0 204 Gma104.1 NA AFSC 0 244 Gma105 NA AFSC 0 172 Gma105.1 NA AFSC 0 182 Gma108 NA AFSC 0 219 Gma108.1 NA AFSC 0 287 Gma109 NA AFSC 0 340 Gma109.1 NA AFSC 0 360 Gmo37 NA AFSC 0 280 Gmo37.1 NA AFSC 0 366 Tch20 NA AFSC 0 176 Tch20.1 NA AFSC 0 212 GmoG13 NA AFSC 0 239 GmoG13.1 NA AFSC 0 247 GmoG16 NA AFSC 0 168 GmoG16.1 NA AFSC 0 176 Gma107 NA AFSC 0 456 Gma107.1 NA AFSC 0 456 Gmo19 NA AFSC 0 214 Gmo19.1 NA AFSC 0 298 GmoC82 NA AFSC 0 136 GmoC82.1 NA AFSC 0 140 GmoC83 NA AFSC 0 102 GmoC83.1 NA AFSC 0 106 Tch13 NA AFSC 0 90 Tch13.1 NA AFSC 0 98 GmoG5 NA AFSC 0 196 GmoG5.1 NA AFSC 0 200