gov.noaa.nmfs.inport:24260
eng
UTF8
dataset
Alaska Fisheries Science Center
7600 Sand Point Way N.E., Building 4
Seattle
WA
98115
USA
http://www.afsc.noaa.gov
WWW:LINK-1.0-http--link
Website
Website for this organization
information
0700-1700 Pacific Time
resourceProvider
Swiney, Katherine
katherine.swiney@noaa.gov
pointOfContact
2024-02-29T00:00:00
ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data
ISO 19115-2:2009(E)
AFSC/RACE/SAP/Swiney: Red king crab fecundity and embryo and larval quality
AFSC/RACE/SAP/Swiney: Red king crab fecundity and embryo and larval quality
publication
NOAA/NMFS/EDM
24260
https://www.fisheries.noaa.gov/inport/item/24260
WWW:LINK-1.0-http--link
Full Metadata Record
View the complete metadata record on InPort for more information about this dataset.
information
Swiney, Katherine
katherine.swiney@noaa.gov
originator
Long, Chris
chris.long@noaa.gov
originator
https://seagrant.uaf.edu/bookstore/pubs/AK-SG-05-03.html
WWW:LINK-1.0-http--link
Citation URL
Donaldson, W. E. and Byersdorfer, S. C. 2005. Biological field techniques for lithodid crabs. Alaska Sea Grant College Program, University of Alaska. 82 pp.
Cited in the methods section
download
https://seagrant.uaf.edu/bookstore/pubs/AK-SG-90-04.html
WWW:LINK-1.0-http--link
Citation URL
Otto,R. S, R. A. MacIntosh, and P. A. Cummiskey. 1990. ¿Fecundity and other reproductive parameters of female red king crab (Paralithodes camtschatica) in Bristol Bay and Norton Sound, Alaska. International Symposium on King and Tanner crabs, Alaska Sea Grant AK0SG-90-04. 65-90 pp.
Manuscript cited in the methods section
download
http://www.bioone.org/doi/pdf/10.2983/035.034.0233
WWW:LINK-1.0-http--link
Citation URL
Swiney, K. M. and Long, W. C. 2015. Primiparious red king crab Paralithodes camtschaticus are less fecund than multiparous crab. Journal of Shellfish Research 34(2): 493-498.
Manuscript produced from this data
download
http://booksandjournals.brillonline.com/content/journals/10.1163/1937240x-00002162
WWW:LINK-1.0-http--link
Citation URL
Swiney, K. M., Eckert, G. L., and Kruse, G. H.Does maternal size affect red king crab, Paralithodes camtschaticus, embryo and larval quality? Journal of Crustacean Biology 33(4): 470-480.
Manuscript produced from this data
download
https://seagrant.uaf.edu/bookstore/pubs/AK-SG-10-01.html
WWW:LINK-1.0-http--link
Citation URL
Swiney, K. M., J. B. Webb, G. H. Bishop, and G. L. Eckert. 2010. ¿Temporal and spatial variability of Alaska red king crab fecundity, and accuracy of clutch fullness indices in estimating fecundity. Biology and Management of Exploited Crab Populations under Climate Change. Alaska Sea Grant AK-SG-10-01. 265-282 pp.
Manuscript produced from this data
download
http://www.bioone.org/doi/pdf/10.2983/035.031.0403
WWW:LINK-1.0-http--link
Citation URL
Swiney, K. M., Long, W. C., Eckert, G. L., and Kruse, G. H. 2012. Red king crab, Paralithodes camtschaticus, size-fecundity relationship, and interannual and seasonal variability in fecundity. Journal of Shellfish Research 31(4): 925-933.
Manuscript produced from this data
download
Stock assessment of Alaskan red king crab, Paralithodes camtschaticus (Tilesius, 1815), can be improved by incorporating reproductive output, which requires an understanding of the size-fecundity relationship, interannual and seasonal variability in fecundity, and maternal size effects on embryo and larval quality. We collected red king crab egg clutches from Bristol Bay, Alaska, in summer of 2007-2010 and autumn of 2007-2009 and estimated fecundity. A monitoring project examining the size-fecundity relationship began in 2012 and data is collected every other year.In June 2009 and 2010 we collected embryo clutches of recently extruded red king crab embryos in Bristol Bay, Alaska, to assess embryo quality based on dry weight, carbon and nitrogen content. To assess larval quality, we collected ovigerous females from Bristol Bay in 2007 and reared them in the laboratory until larval hatching in 2008. Larval quality based on dry weight, carbon and nitrogen content, and time to 50% mortality under starvation conditions was assessed.
The purpose of this study was to estimate the size-fecundity relationship and interannual and seasonal variability in fecundity of Bristol Bay red king crab during 2007-2010 and to determine whether red king crab maternal size affects embryo or larval quality, and if embryo quality varies annually. This data increases our understanding of the processes and functional relationships affecting embryo production, provide data critical to stock assessment models for improved management, and establish a baseline against which monitoring can be used to test for environmental or fishing-induced effects on stock reproductive potential. In 2012 a monitoring project started and samples are collected every other year.
Funded by North Pacific Research Board, National Marine Fisheries Service, Alasks Department of Fish and Game
underDevelopment
Swiney, Katherine
katherine.swiney@noaa.gov
pointOfContact
Swiney, Katherine
katherine.swiney@noaa.gov
email
custodian
asNeeded
embryo production
embryo quality
fecundity
larval quality
maternal size effects
red king crab
reproductive output
size-fecundity relationship
theme
Alaska
Bristol Bay
Eastern Bering Sea
place
DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce
dataCentre
Global Change Master Directory (GCMD) Data Center Keywords
2017-04-24
publication
8.5
Shellfish Assessment Program
project
InPort
otherRestrictions
Cite As: Alaska Fisheries Science Center, [Date of Access]: AFSC/RACE/SAP/Swiney: Red king crab fecundity and embryo and larval quality [Data Date Range], https://www.fisheries.noaa.gov/inport/item/24260.
NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
otherRestrictions
Access Constraints: Contact Point Of Contact for data request form.
otherRestrictions
Use Constraints: User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested.
otherRestrictions
Distribution Liability: The user is responsible for the results of any application of this data for other than its intended purpose. NOAA denies liability if the data are misused.
unclassified
NOAA Data Management Plan (DMP)
NOAA/NMFS/EDM
24260
https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/24260.pdf
WWW:LINK-1.0-http--link
NOAA Data Management Plan (DMP)
NOAA Data Management Plan for this record on InPort.
information
crossReference
eng; US
oceans
Excel
-168
-157
54.6
58.65
Bristol Bay, Alaska.
| Currentness: Ground Condition
2007-06-01
2012-07-01
false
eng
false
Fecundity and embryo quality
2016-05-16
publication
Egg viability
2016-05-16
publication
Larval quality
2016-05-16
publication
Swiney, Katherine
katherine.swiney@noaa.gov
email
distributor
https://console.cloud.google.com/storage/browser/nmfs_odp_afsc/RACE/SAP/Swiney%3B%20Red%20king%20crab%20fecundity%20and%20embryo%20and%20larval%20quality
WWW:LINK-1.0-http--link
https://console.cloud.google.com/storage/browser/nmfs_odp_afsc/RACE/SAP/Swiney%3B%20Red%20king%20crab%20fecundity%20and%20embryo%20and%20larval%20quality
Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/16/2021. Contact: Daniel.woodrich@noaa.gov
download
dataset
Accuracy
Data were double checked prior to analysis. Outliers were remvoed from the dataset.
Completeness Report
Our goal was to collected a certain number of crab per size bin. Sometimes enough crab were not encountered that meet our criteria so all of the size bins were not filled each year. The nitrogen content of embryos from one female in 2010 was twice as high as those for all others, so it was considered an outlier and was dropped from the dataset. Female size, clutch size (NMFS), and clutch fullness (ADFG) were occassinally not recorded.
Conceptual Consistency
not applicable
To compare inter-annual and seasonal variability in red king crab fecundity, egg clutches of ovigerous females were collected from Bristol Bay, Alaska, in summer and autumn. Summer samples were collected during June to July 2007, 2008, 2009, 2010 and 2012 during bottom trawl surveys conducted by NMFS and autumn samples were collected during October to November 2007, 2008, and 2009 by observers from sampled bycatch aboard pot (trap) vessels during the commercial fishery. In summer 2007, ovigerous females were sampled from 10-mm size bins between 90 and 140 mm CL and samples were preserved at sea in 10% buffered formalin. For the rest of the collections, ovigerous females were sampled from 10-mm size bins between 80 and 150+ mm CL and samples were frozen at sea. For all of the collections, ovigerous females were haphazardly collected until 20 clutches per size bin were attained regardless of clutch size. In summer samples, only females that recently extruded eggs and were brooding uneyed embryos were collected. Samples were collected by either carefully removing the abdominal flap with attached embryos and placing the abdomen in a cloth bag or by placing the entire female with the legs removed in a plastic bag. In either case, the bags were closed in a way to prevent embryo loss. Upon collection, female carapace length were measured to the nearest 1.0 mm, and clutch fullness were recorded using the methods of Donaldson and Byersdorfer (2005).
Fecundity was determined using dry weight methods modified from Otto et al. (1990). Embryos were carefully stripped off the pleopods and then two random samples of 250 embryos were counted. The subsamples and remaining embryos were dried at 60°C until a constant weight was achieved. Fecundity was estimated by dividing the total dry weight of embryos by the average of the two estimates of individual embryo dry weight obtained from the subsamples.
To determine the percentage of embryos that was viable, clutches were examined for the presence of non-viable embryos in summer 2008 and 2009 and autumn 2007, 2008, and 2009. Non-viable eggs were identified by abnormal shape, color, asymmetrical cell cleavage in summer samples and absence of an eyed-embryo in autumn samples. For recently extruded eggs, freezing and formalin preservation prevented determination of embryo viability. So, for summer collections a random sample of fresh embryos were removed from a subset of the females collected for fecundity estimation and placed in labeled vials of seawater. Vials were kept chilled while at sea and examined under a dissecting microscope immediately upon arrival at the laboratory. In the autumn, all clutches with eyed embryos were examined for the percentage of viable embryos which was determined from the two subsamples of 250 embryos counted for fecundity estimation. If embryo eyes could not be seen macroscopically, then embryos were examined microscopically to determine viability.
To examine maternal size effects and inter-annual variability in embryo quality, embryo clutches of ovigerous females were collected from Bristol Bay, Alaska, in June 2009 and 2010 during the National Marine Fisheries Service (NMFS) bottom trawl survey in the eastern Bering Sea. A subset of samples collected for the fecundity study were examined for embryo quality. Ten samples from 3 size bins (86-95 mm, 111-120 mm, and 136-145 mm CL) for a total of 30 were randomly chosen from the fecundity collections. Only females that recently extruded eggs and were brooding uneyed embryos were chosen. Samples were collected at sea by carefully removing the abdominal flap with embryos attached and placing the abdomen into a sealed cloth bag to ensure that no embryos were lost, and then frozen.
We assessed embryo quality by measuring embryo dry weights, carbon content, and nitrogen content. To estimate embryo dry weight and fecundity, embryos were carefully stripped off of the pleopods and then two random samples of 250 embryos each were counted. The subsamples and remaining clutch were dried at 60°C until a constant weight was achieved. Individual embryo weights were calculated for each subsample and averaged to obtain the mean embryo dry weight for an individual crab. Fecundity was estimated by dividing the total dry weight of all embryos by the mean embryo dry weight. From each female, a few hundred embryos were dried, finely ground to a powder, and approximately 0.2 g of sample were sent to an analytical laboratory for carbon and nitrogen content analysis using the Dumas combustion method with an automated organic elemental analyzer (Gnaiger and Bitterlich, 1984).
To assess maternal size effects on larval quality, live ovigerous red king crab were collected from Bristol Bay, Alaska, November 2007 by onboard observers during the commercial fishery. Crab were shipped to the NMFS Kodiak Seawater Laboratory where they were reared in a tank with flow-through, sand-filtered seawater. The seawater was chilled to approximate monthly average Bristol Bay bottom temperatures ranging from 1.7 to 4.4° C (mean = 2.7° C, SD = 0.8). Crab were fed ad libitum a diet of fish and squid biweekly. At the beginning of April 2008 each crab was transferred to an individual tub with flow-through seawater and netted outflows to monitor hatching. When an individual crab hatched approximately 500 larvae, the female was moved to a non-flow-through tub overnight to collect viable larvae for experiments; larvae were damaged in nets of the flow-through tubs and therefore not viable for experiments. The tub was placed in a bigger tank with flowing water that served as a water bath to maintain the proper water temperature in the tub; additionally an airstone was placed in the tub to oxygenate the water. In the morning active, phototactic larvae were collected for the experiments. Larvae from 35 females of sizes 93-134 mm CL were used in starvation survival experiments to measure larval fitness. Twenty-five larvae from each female were collected and used in each of 3 replicates, for a total of 75 larvae per each of the 35 females. For each replicate, 25 larvae were placed in a 10.16 cm diameter PVC tube with 650 micron mesh glued to the bottom. The PVC tubes were placed in 2 L beakers filled with UV sterilized filtered seawater. The experiments were conducted in a cold room at 8° C with a cycle of 12 hours light (1.4 lux) and 12 hours dark. Water was changed biweekly and larvae were not fed. Beakers were checked daily for molts and mortalities. A larva was considered dead when it had no color and did not move when observed in a pipette for 10 seconds. Lethal time to 50% mortality (LT50) was calculated as the average number of days when at least 50% mortality was observed. Larvae from each of the female crab used in the starvation experiment were collected upon hatching and dried at 60°C until a constant weight was achieved for measurements of dry weight and carbon and nitrogen analysis. Twenty dried larvae per female were weighed individually and averaged to estimate mean larval weight at the start of the survival experiments. From each female, a few hundred larvae were dried, finely ground to a powder, and approximately 0.2 g of sample were sent to an analytical laboratory for carbon and nitrogen content analysis, as described above for embryos.