25107 Ocean acidification impacts on fish embryos Data Set Published / External 23141 Aquaculture Systems & Ecology Branch (ASEB) Project Completed Black sea bass (Centropristis striata) and scup (Stenotomus chrysops) compose important recreational and commercial fisheries along the United States Atlantic coast. Black sea bass is a temperate species, associated with reef habitat. Wild stocks and landings have been decreasong in recent decades. The demand for black sea bass exceeds supply, and the high market value has prompted research to evaluate their potential for commercial aquaculture. Recent studies conducted at the National Marine Fisheries Service, Milford, CT laboratory examined growth rates of juvenile scup fed commercial diets. This and other on-going studies at Milford have shown scup to acclimate quickly to tank conditions in the laboratory, and to exhibit rapid growth rates. These studies indicate the possibility that scup have potential as a candidiate species for commercial aquaculture. Studies with both fish species suggest they are interesting species for studies of the effects of ocean acidification because of their economic importance as fisheries species. These studies focused on laboratory-based experiments to measure the biological effects of elevated levels of CO2 on embryos of these important marine finfish. Adult black sea bass were naturally conditioned and spawned in the laboratory by photo-thermal manipulation. Adult scup were strip-spawned at sea and their eggs were fertilized at sea. The fertilized eggs of both species of fish were exposed to two treatment levels of pCO2 and one control level, with three replicates per treatment and the controls. Measurements of biological effects included percent hatch, viable embryos, abnormal embryos, and dead embryos. Measurements of dissolved oxygen concentration, percent oxygen saturation, temperature, salinity and pH were taken daily in each treatment container and the controls. Samples of seawater were taken at the time of intial experimental setup and at the time of hatching from each container for analyses of dissolved inorganic carbon (DIC), and analyses of pH by spectrometry. To determine impacts of ocean acidification on embryos of two species of marine fish. Theme Ocean acidification Theme abnormal Theme black sea bass Theme carbon dioxide concentration Theme dissolved inorganic carbon Theme dissolved oxygen concentration Theme embryos Theme experimental Theme normal Theme pH Theme percent oxygen saturation Theme salinity Theme scup Theme temperature Theme total hatch Temporal Summer Spatial Northwest Atlantic Stratum pelagic NEFSC Milford Lab Milford CT Data Set MS Excel Spreadsheet Daily Table (digital) Dissecting microscope, DIC analyzer, YSI, UV spectrophotometer Scientific laboratory 500 micron nytex screen 32802 O.A. black sea bass embryo & larval samples 2013 trial1 larval trial Published / External Completed BSB exposed to varying levels of CO2 during summer 2013. There are two worksheets in this format: Trial 1 - exp 7/11-7/13, stocked at 200/bag Normal, abnormal, and total hatched black sea bass embryos exposed to increased levels of CO2 in laboratory experiments. Larval trial - exp 7/11-7/16. stocked at 100/bag, 1 control stocked at 86 Normal, abnormal, and total hatched black sea bass larvae exposed to increased levels of CO2 in laboratory experiments. Loaded by batch 8876, 05-24-2016 13:33 Spreadsheet Yes BSB exposed to varying levels of CO2 during summer 2013. There are two worksheets in this format: Trial 1 - exp 7/11-7/13, stocked at 200/bag Normal, abnormal, and total hatched black sea bass embryos exposed to increased levels of CO2 in laboratory experiments. Larval trial - exp 7/11-7/16. stocked at 100/bag, 1 control stocked at 86 Normal, abnormal, and total hatched black sea bass larvae exposed to increased levels of CO2 in laboratory experiments. 1 Date, mean, comments CHARACTER 10 Yes No Active For data rows, this column contains the date; For summary rows, this column contains the description "mean" for the rows that are the mean values for the date, other rows may contain comments CHARACTER 2 Tank # NUMBER 1 Yes No 1 0 Active Aquarium number NUMBER 3 Staight NUMBER 2 Yes No 2 0 Active Number of normal straight embryos analyzed NUMBER 4 Curved NUMBER 2 Yes No 2 0 Active Number of abnormally curved embryos analyzed NUMBER 5 Total hatched NUMBER 2 Yes No 2 0 Active Number of embryos that hatched NUMBER 6 Embryos NUMBER 2 Yes No 2 0 Active Number of embryos that did not hatch NUMBER 7 Total hatched & embryo NUMBER 2 Yes No 2 0 Active Total of the hatched and unhatched embryos NUMBER 8 Counted by CHARACTER 6 Yes No 5 1 Active Two scientists independently analyzed the same embryos for comparison of results CHARACTER 9 PCO2 @ 20 C NUMBER 6 No No 5 1 Active Calculated level of pCO2 at 20°C that the embryos and larvae were exposed to in the experimental trials NUMBER 10 PCO2 @ 25 C NUMBER 6 No No 5 1 Active Calculated level of pCO2 at 25°C (embryos & larvae were not exposed at 25°C NUMBER 32803 O.A. black sea bass embryo & larval samples 2013 trial2 trial3 Published / External Completed Black sea bass embryos exposed to varying levels of CO2 during summer 2013. Number of normal, abnormal and total hatched in laboratory experimental trials. There are two worksheets in this format: Trial 2 - exp 7/18-7/20, stocked at 50/bag Trial 3 - exp 7/20-7/22, stocked at 50/bag Loaded by batch 8876, 05-24-2016 13:33 Spreadsheet Yes Black sea bass embryos exposed to varying levels of CO2 during summer 2013. Number of normal, abnormal and total hatched in laboratory experimental trials. There are two worksheets in this format: Trial 2 - exp 7/18-7/20, stocked at 50/bag Trial 3 - exp 7/20-7/22, stocked at 50/bag 1 Date, mean CHARACTER 10 Yes No Active For data rows, this column contains the date; For summary rows, this column contains the description "mean" for the rows that are the mean values for the date CHARACTER 2 Tank # NUMBER 1 Yes No 1 0 Active Aquarium number NUMBER 3 Staight NUMBER 2 Yes No 2 0 Active Number of normal straight embryos analyzed NUMBER 4 Curved NUMBER 2 Yes No 2 0 Active Number of abnormally curved embryos analyzed NUMBER 5 Total hatched NUMBER 2 Yes No 2 0 Active Number of embryos that hatched NUMBER 6 Embryos NUMBER 2 Yes No 2 0 Active Number of embryos that did not hatch NUMBER 7 Total hatched & embryo NUMBER 2 Yes No 2 0 Active Total of the hatched and unhatched embryos NUMBER 8 Counted by: CHARACTER 6 Yes No 5 1 Active Two scientists independently analyzed the same embryos for comparison of results CHARACTER 9 PCO2 @20 C [date1] NUMBER 6 No No 5 1 Active Calculated level of pCO2 at 20°C that the embryos and larvae were exposed to in the experimental trials NUMBER 10 PCO2 @20 C [date2] NUMBER 6 No No 5 1 Active Calculated level of pCO2 at 20°C that the embryos and larvae were exposed to in the experimental trials NUMBER 11 mean PCO2 @20 C NUMBER 6 No No 5 1 Active Mean level of pCO2 from 3 replicate aquariums containing embryos and larvae NUMBER 12 PCO2 @25 C [date1] NUMBER 6 No No 5 1 Active Calculated level of pCO2 at 25°C (embryos & larvae were not exposed at 25°C NUMBER 13 PCO2 @25 C [date2] NUMBER 6 No No 5 1 Active Calculated level of pCO2 at 25°C (embryos & larvae were not exposed at 25°C NUMBER 14 mean PCO2 @25 C NUMBER 6 No No 5 1 Active Mean level of pCO2 calcuated at 25°C (embryos & larvae were not exposed at 25°C NUMBER 32805 OA Larval hatch rates 2014 date Published / External Completed Data on normal, abnormal, and total hatched black sea bass embryos exposed to increased levels of CO2 in laboratory experiments Loaded by batch 8879, 05-24-2016 15:11 Spreadsheet Yes Data on normal, abnormal, and total hatched black sea bass embryos exposed to increased levels of CO2 in laboratory experiments 1 Date DATE 10 Yes No Active For data rows, this column contains the date; For summary rows, this column contains the description "mean" for the rows that are the mean values for the date DATE 2 Tank # NUMBER 1 Yes No 1 0 Active Aquarium number NUMBER 3 Straight NUMBER 2 Yes No 2 0 Active Number of normal straight embryos analyzed NUMBER 4 Curved NUMBER 2 Yes No 2 0 Active Number of abnormally curved embryos analyzed NUMBER 5 Total hatched NUMBER 2 Yes No 2 0 Active Number of embryos that hatched NUMBER 6 Embryos NUMBER 2 Yes No 2 0 Active Number of embryos that did not hatch NUMBER 7 Total hatched & embryo NUMBER 2 Yes No 2 0 Active Total of the hatched and unhatched embryos NUMBER 8 Counted by CHARACTER 2 Yes No Active Two scientists independently analyzed the same embryos for comparison of results. CHARACTER 32806 OA Larval hatch rates 2014 means Published / External Completed Mean number of normal, abnormal, and total hatched black sea bass embryos from 3 replicate aquariums exposed to increased levels of CO2 in laboratory experiments Loaded by batch 8879, 05-24-2016 15:11 Spreadsheet Yes Mean number of normal, abnormal, and total hatched black sea bass embryos from 3 replicate aquariums exposed to increased levels of CO2 in laboratory experiments 1 O.A. Date DATE 10 Yes No Active For data rows, this column contains the date; For summary rows, this column contains the description "mean" for the rows that are the mean values for the date DATE 2 O.A. Tank # NUMBER 1 Yes No 1 0 Active Aquarium number NUMBER 3 O.A. Straight NUMBER 2 Yes No 2 0 Active Number of normal straight embryos analyzed. Mean values are data scale = 2 . NUMBER 4 O.A. Curved NUMBER 2 Yes No 2 0 Active Number of abnormally curved embryos analyzed. Mean values are data scale = 2 . NUMBER 5 O.A. Total hatched NUMBER 2 Yes No 2 0 Active Number of embryos that hatched. Mean values are data scale = 2 . NUMBER 6 O.A. Embryos NUMBER 2 Yes No 2 0 Active Number of embryos that did not hatch. Mean values are data scale = 2 . NUMBER 7 O.A. Total hatched & embryo NUMBER 2 Yes No 2 0 Active Total of the hatched and unhatched embryos. Mean values are data scale = 2 . NUMBER 8 O.A. Counted by CHARACTER 2 Yes No Active Initials of individual taking count or "mean" if row contains mean values for the date CHARACTER 9 Blank1 CHARACTER 1 No No Active blank column CHARACTER 10 Blank2 CHARACTER 1 No No Active blank column CHARACTER 11 Means Date DATE 10 Yes No Active For data rows, this column contains the date; For summary rows, this column contains the description "mean" for the rows that are the mean values for the date DATE 12 Means Tank # NUMBER 6 Yes No 5 2 Active Aquarium number NUMBER 13 Means Straight NUMBER 6 Yes No 5 2 Active Number of straight embryos from 3 replicate aquariums NUMBER 14 Means Curved NUMBER 6 Yes No 5 2 Active Number of curved embryos from 3 replicate aquariums NUMBER 15 Means Total hatched NUMBER 6 Yes No 5 2 Active Number of hatched embryos from 3 replicate aquariums NUMBER 16 Means Embryos NUMBER 6 Yes No 5 2 Active Number of embryos that did not hatch from 3 replicate aquariums NUMBER 17 Means Total hatched & embryo NUMBER 6 Yes No 5 2 Active Number of hatched and unhatched embryos from 3 replicate aquariums NUMBER 18 Means Counted by CHARACTER 2 Yes No Active Initials of individual taking count or "mean" if row contains mean values for the date CHARACTER 32807 OA Larval hatch rates 2014 treatment Published / External Completed Data on number of normal, abnormal, and total hatched black sea bass embryos exposed to increased levels of CO2 in laboratory experiments by treatment level. Loaded by batch 8879, 05-24-2016 15:11 Spreadsheet Yes Data on number of normal, abnormal, and total hatched black sea bass embryos exposed to increased levels of CO2 in laboratory experiments by treatment level. 1 Blank1 CHARACTER 1 No No Active This column left blank CHARACTER 2 Tank 1- 3 Date DATE 10 Yes No Active For data rows, this column contains the date; For summary rows, this column contains the description "treatment" or "mean" for the rows that are the mean values for the column DATE 3 Tank 1- 3 Tank # NUMBER 1 Yes No 1 0 Active Replicate aquariums of fish embryos exposed to the control level of CO2 used in laboratory experiments NUMBER 4 Tank 1- 3 Straight NUMBER 5 Yes No 4 2 Active Number of straight embryos from the 3 replicate aquariums of fish embryos exposed to the control level of CO2 NUMBER 5 Tank 1- 3 Curved NUMBER 5 Yes No 4 2 Active Number of curved embryos from the 3 replicate aquariums of fish embryos exposed to the control level of CO2 NUMBER 6 Tank 1- 3 Total hatched NUMBER 5 Yes No 4 2 Active Number of total hatched embryos from the 3 replicate aquariums of fish embryos exposed to the control level of CO2 NUMBER 7 Tank 1- 3 Embryos NUMBER 5 Yes No 4 2 Active Number of embryos that did not hatch from 3 replicate aquariums of fish embryos exposed to the control level of CO2 NUMBER 8 Tank 1- 3 Total hatched & embryos NUMBER 6 Yes No 5 2 Active Total of the hatched and unhatched embryos from 3 replicate aquariums of fish embryos exposed to the control level of CO2 NUMBER 9 Blank2 CHARACTER 1 No No Active This column left blank CHARACTER 10 Blank3 CHARACTER 1 No No Active This column left blank CHARACTER 11 Tank 4-6 Date DATE 10 Yes No Active For data rows, this column contains the date; For summary rows, this column contains the description "treatment" or "mean" for the rows that are the mean values for the column DATE 12 Tank 4-6 Tank # NUMBER 1 Yes No 1 0 Active Replicate aquariums of fish embryos exposed to the mid-level of CO2 used in laboratory experiments NUMBER 13 Tank 4-6 Straight NUMBER 5 Yes No 4 2 Active Number of straight embryos from the 3 replicate aquariums of fish embryos exposed to the mid-level of CO2 NUMBER 14 Tank 4-6 Curved NUMBER 5 Yes No 4 2 Active Nmber of curved embryos from the 3 replicate aquariums of fish embryos exposed to the mid-level of CO2 NUMBER 15 Tank 4-6 Total hatched NUMBER 5 Yes No 4 2 Active Number of total hatched embryos from the 3 replicate aquariums of fish embryos exposed to the mid-level of CO2 NUMBER 16 Tank 4-6 Embryos NUMBER 5 Yes No 4 2 Active Number of embryos that did not hatch from 3 replicate aquariums of fish embryos exposed to the mid-level of CO2 NUMBER 17 Tank 4-6 Total hatched & embryos NUMBER 6 Yes No 5 2 Active Total of the hatched and unhatched embryos from 3 replicate aquariums of fish embryos exposed to the mid-level of CO2 NUMBER 18 Blank4 CHARACTER 1 No No Active This column left blank CHARACTER 19 Tank 7-9 Date DATE 10 Yes No Active For data rows, this column contains the date; For summary rows, this column contains the description "treatment" or "mean" for the rows that are the mean values for the column DATE 20 Tank 7-9 Tank # NUMBER 5 Yes No 4 2 Active Replicate aquariums of fish embryos exposed to the high level of CO2 used in laboratory experiments NUMBER 21 Tank 7-9 Straight NUMBER 5 Yes No 4 2 Active Number of straight embryos from the 3 replicate aquariums of fish embryos exposed to the high level of CO2 NUMBER 22 Tank 7-9 Curved NUMBER 5 Yes No 4 2 Active Number of curved embryos from the 3 replicate aquariums of fish embryos exposed to the high level of CO2 NUMBER 23 Tank 7-9 Total hatched NUMBER 5 Yes No 4 2 Active Number of total hatched embryos from the 3 replicate aquariums of fish embryos exposed to the high level of CO2 NUMBER 24 Tank 7-9 Embryos NUMBER 6 Yes No 5 2 Active Number of embryos that did not hatch from 3 replicate aquariums of fish embryos exposed to the high level of CO2 NUMBER 25 Tank 7-9 Total hatched & embryos NUMBER 2 Yes No 2 0 Active Total of the hatched and unhatched embryos from 3 replicate aquariums of fish embryos exposed to the high level of CO2 NUMBER 32808 OA 2015 black seabass counts 7-10-2015 Published / External Completed Data on normal, abnormal, and total hatched black sea bass embryos exposed to increased levels of CO2 in laboratory experiments Loaded by batch 8880, 05-24-2016 16:05 Spreadsheet Yes Data on normal, abnormal, and total hatched black sea bass embryos exposed to increased levels of CO2 in laboratory experiments 1 Date CHARACTER 10 Yes No Active For data rows, this column contains the date; For summary rows, this column contains the description "mean" for the rows that are the mean values for the date CHARACTER 2 Tank # NUMBER 1 Yes No 1 0 Active Aquarium number NUMBER 3 Straight NUMBER 2 Yes No 2 0 Active Number of normal straight embryos analyzed. Mean is calculated with decimal scale = 2. NUMBER 4 Curved NUMBER 2 Yes No 2 0 Active Number of abnormally curved embryos analyzed. Mean is calculated with decimal scale = 2. NUMBER 5 Total hatched NUMBER 2 Yes No 2 0 Active Number of embryos that hatched. Mean is calculated with decimal scale = 2. NUMBER 6 Embryos NUMBER 2 Yes No 2 0 Active Number of embryos that did not hatch. Mean is calculated with decimal scale = 2. NUMBER 7 Total hatched & embryo NUMBER 2 Yes No 2 0 Active Total of the hatched and unhatched embryos. Mean is calculated with decimal scale = 2. NUMBER 8 Dead eggs NUMBER 2 Yes No 2 0 Active Number of embryos that were dead. Mean is calculated with decimal scale = 2. NUMBER 9 Total NUMBER 3 Yes No 3 0 Active Total number of embryos analyzed. Mean is calculated with decimal scale = 2. NUMBER 10 Counted by CHARACTER 2 No No 0 0 Active Two scientists independently analyzed the same embryos for comparison of results. CHARACTER 32809 OA 2015 scup counts 6-25-2015 trial 1 Published / External Completed Data on normal, abnormal, and total hatched scup embryos exposed to increased levels of CO2 in laboratoty experiments Loaded by batch 8881, 05-24-2016 16:39 Spreadsheet Yes Data on normal, abnormal, and total hatched scup embryos exposed to increased levels of CO2 in laboratoty experiments 1 Date CHARACTER 10 Yes No Active For data rows, this column contains the date the embryos were analyzed; For summary rows, this column contains the description "mean" for the rows that are the mean values for the date CHARACTER 2 Tank # NUMBER 1 Yes No 1 0 Active Aquarium number NUMBER 3 Straight NUMBER 2 Yes No 2 0 Active Number of normal straight embryos analyzed. Mean is calculated with decimal scale = 2. NUMBER 4 Curved NUMBER 2 Yes No 2 0 Active Number of abnormally curved embryos analyzed. Mean is calculated with decimal scale = 2. NUMBER 5 Total hatched NUMBER 2 Yes No 2 0 Active Number of embryos that hatched. Mean is calculated with decimal scale = 2. NUMBER 6 Embryos NUMBER 2 Yes No 2 0 Active Number of embryos that did not hatch. Mean is calculated with decimal scale = 2. NUMBER 7 Total hatched & embryo NUMBER 2 Yes No 2 0 Active Total of the hatched and unhatched embryos. Mean is calculated with decimal scale = 2. NUMBER 8 Dead eggs NUMBER 2 Yes No 2 0 Active Number of embryos that were dead. Mean is calculated with decimal scale = 2. NUMBER 9 Total NUMBER 2 Yes No 2 0 Active Total number of embryos analyzed. Mean is calculated with decimal scale = 2. NUMBER 10 Counted by CHARACTER 2 No No 0 0 Active Two scientists independently analyzed the same embryos for comparison of results. CHARACTER 32810 OA 2015 scup counts 6-28-2015 trial 2 Published / External Completed Data on normal, abnormal, and total hatched scup embryos exposed to increased levels of CO2 in laboratory experiments Loaded by batch 8883, 05-24-2016 17:02 Spreadsheet Yes Data on normal, abnormal, and total hatched scup embryos exposed to increased levels of CO2 in laboratory experiments 1 Date CHARACTER 10 Yes No Active For data rows, this column contains the date; For summary rows, this column contains the description "mean" for the rows that are the mean values for the date CHARACTER 2 Tank # NUMBER 1 Yes No 1 0 Active Aquarium number NUMBER 3 Straight NUMBER 2 Yes No 2 0 Active Number of normal straight embryos analyzed. Mean is calculated with decimal scale = 2. NUMBER 4 Curved NUMBER 2 Yes No 2 0 Active Number of abnormally curved embryos analyzed. Mean is calculated with decimal scale = 2. NUMBER 5 Total hatched NUMBER 2 Yes No 2 0 Active Number of embryos that hatched. Mean is calculated with decimal scale = 2. NUMBER 6 Embryos NUMBER 2 Yes No 2 0 Active Number of embryos that did not hatch. Mean is calculated with decimal scale = 2. NUMBER 7 Total hatched & embryo NUMBER 2 Yes No 2 0 Active Total of the hatched and unhatched embryos. Mean is calculated with decimal scale = 2. NUMBER 8 Dead eggs NUMBER 2 Yes No 2 0 Active Number of embryos that were dead. Mean is calculated with decimal scale = 2. NUMBER 9 Total NUMBER 2 Yes No 2 0 Active Total number of embryos analyzed. Mean is calculated with decimal scale = 2. NUMBER 10 Counted by CHARACTER 2 No No 0 0 Active Two scientists independently analyzed the same embryos for comparison of results. CHARACTER Data Steward 2013 Person Redman, Dylan Dylan.Redman@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 203-882-6518 203-882-6570 Distributor 2013 Person Redman, Dylan Dylan.Redman@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 203-882-6518 203-882-6570 Metadata Contact 2013 Person Redman, Dylan Dylan.Redman@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 203-882-6518 203-882-6570 Originator 2013 Person Perry, Dean M dean.perry@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 203-882-6530 203-882-6570 Point of Contact 2013 Person Redman, Dylan Dylan.Redman@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 203-882-6518 203-882-6570 Mid-Atlantic region of Northwest Atlantic Ocean, Long Island Sound, Connecticut Range 2013-07 2015-07 Unclassified There will be a digital object identifier and a URL that can be accessed. NEFSC Data Access Procedure: 1. Formal request in writing usually to the data owner/contact or Center Director; 2. Requester is contacted by data owner to review and verify the request content and details for data delivery options. 3. If data is confidential then owner will determine if the data may be released to the requester; 4. If data can be released, the data is downloaded and packaged for delivery electronically; or the requester may be directed to where the data is available online. Data can not be accessed until publication of manuscript in 2017. NEFSC Data Access Constraint: If data is stored on an internal NEFSC network, the requester may not have direct access to query the data unless they are also authorized for network access. https://console.cloud.google.com/storage/browser/nmfs_odp_nefsc/PARR/EAD/ASEB/25107 5 Files: O.A. black sea bass embryo & larval Samples 2013.xlsx OA 2015 black seabass counts 7-10-2015.xlsx OA 2015 scup counts 6-25-2015 trial 1.xlsx OA 2015 scup counts 6-28-2015 trial 2.xlsx OA Larval hatch rates 2014.xlsx MS Excel spreadsheet The accuracy is represented by two experienced scientists conducting visual microscopic observations and checking each others observations for accuracy during the experiment and during data entry. Data validation during data collection and entry: Every sample was analyzed by two scientists for comparison of reliable, consistent results. Portable pH meter used for comparison with UV spec. data. Yes Unknown Yes No Yes not applicable NCEI-MD Unknown Archival of source data preserving unaltered collected data. none 32803 Entity 32802 Entity 32808 Entity 32809 Entity 32810 Entity 32805 Entity 32806 Entity 32807 Entity gov.noaa.nmfs.inport:25107 Patricia Jones 2015-05-22T12:35:23 Michael Armbruster 2023-04-10T15:48:32 2017-02-15 Northeast Fisheries Science Center NEFSC 166 Water Street Woods Hole MA 02543-1026 USA (508)495-2000 https://www.nefsc.noaa.gov 8:00 a.m. to 4:30 p.m. Monday - Friday 1001 Public No No 2017-02-15 1 Year 2018-02-15