26894
AFSC/RACE/SAP/Long: Data from: Embryo development in golden king crab, Lithodes aequispina.
AFSC/RACE/SAP/Long: Data from: Embryo development in golden king crab, Lithodes aequispina.
Data Set
Published / External
22354
Shellfish Assessment Program
Project
In Work
The data from this study, describes embryo development in Golden king crab, Lithodes aequispinus. Six female multiparous golden king crab were captured from the Aleutian Islands, Alaska, and mated in the laboratory. Their embryos were photographed and staged on average once every 9 days throughout embryogenesis.
The purpose of this data is to describe embryogenesis in golden king crab.
Loaded by FGDC Metadata Uploader, batch 7037, 09-04-2015 10:00
The following FGDC sections are not currently supported in InPort, but were preserved and will be included in the FGDC export:
- Taxonomy (FGDC:taxonomy)
Theme
ISO 19115 Topic Category
oceans
Theme
eggs
Theme
reproduction
Spatial
Aleutian Islands
Spatial
Bering Sea
Kodiak Fisheries Research Center
Kodiak
AK
USA
Data Set
As Needed
Table (digital)
We thank B. Stevens for training, sharing techniques, and helpful suggestions, K. Reppond for assistance, and the staff, interns, and volunteers of the Kodiak Seawater Laboratory for assistance in collecting the data.
N/A
N/A
N/A
55556
Crab data
Published / External
Spreadsheet
Crab morphometric data
1
CRABNUMBER
NUMBER
No
No
Active
Unique number assigned to crab
2
SEX
TEXT
No
No
Active
Sex of crab
3
CARAPACELENGTH
NUMBER
No
No
Active
Measurement of carapace length
mm
4
DATEEXTRUDED
DATE
No
No
Active
Date eggs extruded from female
5
MATINGMALE
NUMBER
No
No
Active
Unique number assigned to male crab that mated with female
6
HATCHINGBEGINS
DATE
No
No
Active
Date of egg hatching start
7
HATCHINGENDS
DATE
No
No
Active
Date of egg hatching ending
55557
Embryo Stages
Published / External
Spreadsheet
Embryo brooding data
1
CRABNUMBER
NUMBER
No
No
Active
Unique number assigned female crab with embryos
2
DATE
DATE
No
No
Active
Date at staging
3
BROODINGDAY
NUMBER
No
No
Active
Day in brooding cycle
4
EMBRYOSTAGE
NUMBER
No
No
Active
Blue king crab embryo development based on morphometric staging: Stage 1, day 7, 16 cells; Stage 2, day 28, blastodisc; Stage 3, day 114, “ V ” -embryo; Stage 4, day 157: Stage 6, day 192; Stage 7, day 206; Stage 7, day 206; Stage 8, day 268; Stage 9, day 305; Stage 9, day 305; Stage 11, day 367; Stage 12, day 395, hatching.
55563
Embryo measurements
Published / External
Spreadsheet
Embryo brooding measurement data
1
CRABNUMBER
NUMBER
No
No
Active
Unique number assigned female crab with embryos
2
DATE
DATE
No
No
Active
Date at staging
3
BROODINGDAY
NUMBER
No
No
Active
Day in brooding cycle
4
EMBRYOSTAGE
NUMBER
No
No
Active
Blue king crab embryo development based on morphometric staging: Stage 1, day 7, 16 cells; Stage 2, day 28, blastodisc; Stage 3, day 114, “ V ” -embryo; Stage 4, day 157: Stage 6, day 192; Stage 7, day 206; Stage 7, day 206; Stage 8, day 268; Stage 9, day 305; Stage 9, day 305; Stage 11, day 367; Stage 12, day 395, hatching.
5
EGGAREA
NUMBER
No
No
Active
Calculated area of egg
mm
6
EGGMAXDIAM
NUMBER
No
No
Active
Maximum diameter measurement of egg
mm
7
EGGMINDIAM
NUMBER
No
No
Active
Minimum diameter measurement of egg
mm
8
EGGMEANDIAM
NUMBER
No
No
Active
Mean diameter measurement of egg
mm
9
EMBRYOAREA
NUMBER
No
No
Active
Calculated area of embryo
mm
10
YOLKAREA
NUMBER
No
No
Active
Calculated area of egg yolk
mm
11
EYEAREA
NUMBER
No
No
Active
Calculated area of embryo eye
mm
12
EYEMAXDIAM
NUMBER
No
No
Active
Maximum diameter measurement of embryo eye
mm
13
EYEMINDIAM
NUMBER
No
No
Active
Minimum diameter measurement of embryo eye
14
EYEMEANDIAM
NUMBER
No
No
Active
Mean diameter measurement of embryo eye
mm
15
PRECENTAREAYOLK
NUMBER
No
No
Active
Yolk area calculated as a percent of egg
mm
Data Steward
2015-09-01
Person
Long, Chris
chris.long@noaa.gov
email
Distributor
2015-09-01
Person
Long, Chris
chris.long@noaa.gov
email
Metadata Contact
2015-09-04
Person
MC, Metadata Coordinators
AFSC.metadata@noaa.gov
Point of Contact
2015-09-01
Person
Long, Chris
chris.long@noaa.gov
email
Ground Condition
-152.395268
-152.395268
57.782403
57.782403
Kodiak Fisheries Research Center
Range
2006-09
2008-08
Unclassified
unknown
The data set is still being analyzed and will not be available for distribution until it has been finalized and all QA/QC practices have been performed. Contact the Point Of Contact for estimated time of release.
User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested.
https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/SAP/Long%3B%20Data%20from%3B%20Embryo%20development%20in%20golden%20king%20crab%2C%20Lithodes%20aequispina%3B%20Crab%20Data.csv
Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/15/2021. Contact: Daniel.woodrich@noaa.gov
Excel
Data was checked for outliers and for values out of expected range.
unknown
No
Unknown
No
No
yes
unknown
No delay
NCEI-MD
unknown
IT Security and Contingency Plan for the system establishes procedures and applies to the functions, operations, and resources necessary to recover and restore data as hosted in the Western Regional Support Center in Seattle, Washington, following a disruption.
1
<methtype>Lab</methtype>
<methdesc>Golden king crab for this experiment were caught in commercial pots along the Aleutian Islands, Alaska, in the falls of 2005 and 2006 and transported to the ASFC Kodiak Laboratory via air cargo. Crab were held in 2,000 l tanks with flow-through seawater chilled to 3-4 degrees Celsius, and fed chopped frozen fish and squid to excess. Tanks were covered with opaque sheets of foam to both provide insulation and to keep the females in mostly-dark conditions. Six females, 4 captured in 2005 and 2 in 2006, were used in this study. Females were either late-stage ovigerous, as evinced by eyed embryos, or hatched out, as evinced by empty egg cases when collected and were between 120 and 141 mm in carapace length (CL). Six mature males, CL 128-132 mm, were present with the females in a pre-mating holding tank. After the females molted, they mated and extruded a new clutch of eggs. At this point they were placed into another tank where all the post-mating females were held together with no males.</methdesc>
</method>
<method>
<methtype>Lab</methtype>
<methdesc>Embryos were collected regularly throughout development from a random location within the clutch. Time between samples varied with the developmental stage and averaged once every 9 days or a mean of 48 times (range 42-54) throughout development for each female. At sampling, embryos were examined and photographed under a stereo microscope. Uneyed embryos were stained for 5 min in Bouin's solution to enable staging. The median stage of development (see results for descriptions) was determined for each female on each sampling day. In addition, up to 10 unstained embryos were photographed at 90° to the sagittal plane under a stereo microscope for image analysis. The images were calibrated each day with a micrometer because the scope had an adjustable zoom and this ensured accurate measurements. Measurements were made using Image-Pro Plus V. 7.0 (Media Cybernetics, Inc. , Rockville, MD). In this data, the term egg; refers to the entire embryo (i.e. the entire contents of the fertilized egg) and the term embryo; refers to the differentiated part of the egg as distinct from the yolk, and yolk; refers to the undifferentiated deutoplasm. For each egg, the area and the mean, minimum, and maximum diameter of the egg was measured. The mean, minimum, and maximum diameters were determined from 180 measurements of the diameter at 2 degree intervals around the entire egg. When the embryo became visible, the yolk area was also measured by tracing the yolk in Image-Pro and the percent area yolk (yolk area x 100/egg area; prior to the embryo becoming the yolk area = egg area) and the embryo area (egg area minus the yolk area) were calculated. Finally, when the eyes became visible, the eye area and the mean, minimum, and maximum diameter of the eyes were measured using the same techniques as above.</methdesc>
</method>
55556
Entity
Crab data
55557
Entity
Embryo Stages
55563
Entity
Embryo measurements
gov.noaa.nmfs.inport:26894
Nancy Roberson
2015-09-04T10:00:21
SysAdmin InPortAdmin
2023-05-30T18:09:07
2016-05-16
Alaska Fisheries Science Center
AFSC
7600 Sand Point Way N.E., Building 4
Seattle
WA
98115
USA
http://www.afsc.noaa.gov
0700-1700 Pacific Time
1001
Public
No
2016-05-16
1 Year
2017-05-16
None
invertebrates
single species
crab
Kingdom
Animalia
Animal
animaux
animals
Subkingdom
Bilateria
Infrakingdom
Protostomia
Superphylum
Ecdysozoa
Phylum
Arthropoda
Artrpode
arthropodes
arthropods
Subphylum
Crustacea
crustacs
crustceo
crustaceans
Class
Malacostraca
Subclass
Eumalacostraca
Superorder
Eucarida
camaro
caranguejo
ermito
lagosta
siri
Order
Decapoda
crabs
crayfishes
lobsters
prawns
shrimp
crabes
crevettes
crevisses
homards
Suborder
Pleocyemata
Infraorder
Anomura
Superfamily
Paguroidea
Family
Lithodidae
stone and king crabs
Genus
Lithodes
Species
Lithodes aequispinus
golden king crab