26894 AFSC/RACE/SAP/Long: Data from: Embryo development in golden king crab, Lithodes aequispina. Data Set Published / External 22354 Shellfish Assessment Program Project In Work The data from this study, describes embryo development in Golden king crab, Lithodes aequispinus. Six female multiparous golden king crab were captured from the Aleutian Islands, Alaska, and mated in the laboratory. Their embryos were photographed and staged on average once every 9 days throughout embryogenesis. The purpose of this data is to describe embryogenesis in golden king crab. Loaded by FGDC Metadata Uploader, batch 7037, 09-04-2015 10:00 The following FGDC sections are not currently supported in InPort, but were preserved and will be included in the FGDC export: - Taxonomy (FGDC:taxonomy) Theme ISO 19115 Topic Category oceans Theme eggs Theme reproduction Spatial Aleutian Islands Spatial Bering Sea Kodiak Fisheries Research Center Kodiak AK USA Data Set As Needed Table (digital) We thank B. Stevens for training, sharing techniques, and helpful suggestions, K. Reppond for assistance, and the staff, interns, and volunteers of the Kodiak Seawater Laboratory for assistance in collecting the data. N/A N/A N/A 55556 Published / External Spreadsheet Crab morphometric data 1 CRABNUMBER NUMBER No No Active Unique number assigned to crab 2 SEX TEXT No No Active Sex of crab 3 CARAPACELENGTH NUMBER No No Active Measurement of carapace length mm 4 DATEEXTRUDED DATE No No Active Date eggs extruded from female 5 MATINGMALE NUMBER No No Active Unique number assigned to male crab that mated with female 6 HATCHINGBEGINS DATE No No Active Date of egg hatching start 7 HATCHINGENDS DATE No No Active Date of egg hatching ending 55557 Published / External Spreadsheet Embryo brooding data 1 CRABNUMBER NUMBER No No Active Unique number assigned female crab with embryos 2 DATE DATE No No Active Date at staging 3 BROODINGDAY NUMBER No No Active Day in brooding cycle 4 EMBRYOSTAGE NUMBER No No Active Blue king crab embryo development based on morphometric staging: Stage 1, day 7, 16 cells; Stage 2, day 28, blastodisc; Stage 3, day 114, “ V ” -embryo; Stage 4, day 157: Stage 6, day 192; Stage 7, day 206; Stage 7, day 206; Stage 8, day 268; Stage 9, day 305; Stage 9, day 305; Stage 11, day 367; Stage 12, day 395, hatching. 55563 Published / External Spreadsheet Embryo brooding measurement data 1 CRABNUMBER NUMBER No No Active Unique number assigned female crab with embryos 2 DATE DATE No No Active Date at staging 3 BROODINGDAY NUMBER No No Active Day in brooding cycle 4 EMBRYOSTAGE NUMBER No No Active Blue king crab embryo development based on morphometric staging: Stage 1, day 7, 16 cells; Stage 2, day 28, blastodisc; Stage 3, day 114, “ V ” -embryo; Stage 4, day 157: Stage 6, day 192; Stage 7, day 206; Stage 7, day 206; Stage 8, day 268; Stage 9, day 305; Stage 9, day 305; Stage 11, day 367; Stage 12, day 395, hatching. 5 EGGAREA NUMBER No No Active Calculated area of egg mm 6 EGGMAXDIAM NUMBER No No Active Maximum diameter measurement of egg mm 7 EGGMINDIAM NUMBER No No Active Minimum diameter measurement of egg mm 8 EGGMEANDIAM NUMBER No No Active Mean diameter measurement of egg mm 9 EMBRYOAREA NUMBER No No Active Calculated area of embryo mm 10 YOLKAREA NUMBER No No Active Calculated area of egg yolk mm 11 EYEAREA NUMBER No No Active Calculated area of embryo eye mm 12 EYEMAXDIAM NUMBER No No Active Maximum diameter measurement of embryo eye mm 13 EYEMINDIAM NUMBER No No Active Minimum diameter measurement of embryo eye 14 EYEMEANDIAM NUMBER No No Active Mean diameter measurement of embryo eye mm 15 PRECENTAREAYOLK NUMBER No No Active Yolk area calculated as a percent of egg mm Data Steward 2015-09-01 Person Long, Chris chris.long@noaa.gov email Distributor 2015-09-01 Person Long, Chris chris.long@noaa.gov email Point of Contact 2015-09-01 Person Long, Chris chris.long@noaa.gov email Ground Condition -152.395268 -152.395268 57.782403 57.782403 Kodiak Fisheries Research Center Range 2006-09 2008-08 Unclassified unknown The data set is still being analyzed and will not be available for distribution until it has been finalized and all QA/QC practices have been performed. Contact the Point Of Contact for estimated time of release. User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/SAP/Long%3B%20Data%20from%3B%20Embryo%20development%20in%20golden%20king%20crab%2C%20Lithodes%20aequispina%3B%20Crab%20Data.csv Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/15/2021. Contact: Daniel.woodrich@noaa.gov Excel Data was checked for outliers and for values out of expected range. unknown No Unknown No No yes unknown No delay NCEI-MD unknown IT Security and Contingency Plan for the system establishes procedures and applies to the functions, operations, and resources necessary to recover and restore data as hosted in the Western Regional Support Center in Seattle, Washington, following a disruption. 1 <methtype>Lab</methtype> <methdesc>Golden king crab for this experiment were caught in commercial pots along the Aleutian Islands, Alaska, in the falls of 2005 and 2006 and transported to the ASFC Kodiak Laboratory via air cargo. Crab were held in 2,000 l tanks with flow-through seawater chilled to 3-4 degrees Celsius, and fed chopped frozen fish and squid to excess. Tanks were covered with opaque sheets of foam to both provide insulation and to keep the females in mostly-dark conditions. Six females, 4 captured in 2005 and 2 in 2006, were used in this study. Females were either late-stage ovigerous, as evinced by eyed embryos, or hatched out, as evinced by empty egg cases when collected and were between 120 and 141 mm in carapace length (CL). Six mature males, CL 128-132 mm, were present with the females in a pre-mating holding tank. After the females molted, they mated and extruded a new clutch of eggs. At this point they were placed into another tank where all the post-mating females were held together with no males.</methdesc> </method> <method> <methtype>Lab</methtype> <methdesc>Embryos were collected regularly throughout development from a random location within the clutch. Time between samples varied with the developmental stage and averaged once every 9 days or a mean of 48 times (range 42-54) throughout development for each female. At sampling, embryos were examined and photographed under a stereo microscope. Uneyed embryos were stained for 5 min in Bouin's solution to enable staging. The median stage of development (see results for descriptions) was determined for each female on each sampling day. In addition, up to 10 unstained embryos were photographed at 90&#176; to the sagittal plane under a stereo microscope for image analysis. The images were calibrated each day with a micrometer because the scope had an adjustable zoom and this ensured accurate measurements. Measurements were made using Image-Pro Plus V. 7.0 (Media Cybernetics, Inc. , Rockville, MD). In this data, the term egg; refers to the entire embryo (i.e. the entire contents of the fertilized egg) and the term embryo; refers to the differentiated part of the egg as distinct from the yolk, and yolk; refers to the undifferentiated deutoplasm. For each egg, the area and the mean, minimum, and maximum diameter of the egg was measured. The mean, minimum, and maximum diameters were determined from 180 measurements of the diameter at 2 degree intervals around the entire egg. When the embryo became visible, the yolk area was also measured by tracing the yolk in Image-Pro and the percent area yolk (yolk area x 100/egg area; prior to the embryo becoming the yolk area = egg area) and the embryo area (egg area minus the yolk area) were calculated. Finally, when the eyes became visible, the eye area and the mean, minimum, and maximum diameter of the eyes were measured using the same techniques as above.</methdesc> </method> 55556 Entity 55557 Entity 55563 Entity gov.noaa.nmfs.inport:26894 Nancy Roberson 2015-09-04T10:00:21 SysAdmin InPortAdmin 2025-05-15T19:15:47 2016-05-16 Alaska Fisheries Science Center AFSC 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA https://www.afsc.noaa.gov 0700-1700 Pacific Time 1001 Public No No 2016-05-16 1 Year 2017-05-16 None invertebrates single species crab Kingdom Animalia Animal animaux animals Subkingdom Bilateria Infrakingdom Protostomia Superphylum Ecdysozoa Phylum Arthropoda Artrpode arthropodes arthropods Subphylum Crustacea crustacs crustceo crustaceans Class Malacostraca Subclass Eumalacostraca Superorder Eucarida camaro caranguejo ermito lagosta siri Order Decapoda crabs crayfishes lobsters prawns shrimp crabes crevettes crevisses homards Suborder Pleocyemata Infraorder Anomura Superfamily Paguroidea Family Lithodidae stone and king crabs Genus Lithodes Species Lithodes aequispinus golden king crab