AFSC/RACE/SAP/Foy: Seawater chemistry of three pH treatments used to test the effect of pH on Tanner crab biology: Kodiak Island, Alaska.

To study the effects of ocean acidification we examined the effects of ocean acidification on the embryo and larval stages of the economically important southern Tanner crab,

Chionoecetes bairdi. Ovigerous females were reared in one of 3 treatments: control (ambient pH ~8.1), pH 7.8, and pH 7.5 for 2 years. Embryos and larvae in year 1 were from oocytes

developed in the field whereas embryos and larvae in year 2 were from oocytes developed under acidified conditions Larvae hatched each year, were also exposed to 3 pH treatments to

examine starvation-survival, morphology, condition, and calcium/magnesium content. The carbonate chemistry data included salinity, temperature, pH, alkalinity, dissolved inorganic

carbon, CO2, bicarbonate, carbonate, and the saturation states of aragonite and calcite. These values varied according to treatment and season throughout the study.

The purpose of the Carbonate Chemistry dataset is to provide the water chemistry associated with the three treatments throughout the experiment.

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The following FGDC sections are not currently supported in InPort, but were preserved and will be included in the FGDC export:

- Taxonomy (FGDC:taxonomy)

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Must read and fully comprehend the metadata prior to data use; acknowledgement of the Originator when using the data set as a source;

sharing of data products developed using the source data set with the Originator; data should not be used beyond the limits of the source scale;

the data set is NOT a survey document and should not be utilized as such

MS Excel 2012

The accuracy of the Carbonate Chemistry dataset is high (less than 1% difference from actual) due to replicate sampling, measurement accuracy and precision

controlled through system calibrations, and frequency of observations during the experiment.

The Carbonate Chemistry data are filtered so that replicates are averaged or extreme values are reconsidered if greater than 2 SD from the mean.

Carbonate Chemistry information was broadly compared to previous studies and to similar seawater collections made in the Gulf of Alaska while taking into account

laboratory conditions and statistically compared to a control (alpha=0.05). For example, for comparing we used the study described in Mathis, Jeremy T., Jessica N. Cross,

and Nicholas R. Bates. "Coupling primary production and terrestrial runoff to ocean acidification and carbonate

mineral suppression in the eastern Bering Sea." Journal of Geophysical Research: Oceans (19782012) 116.C2 (2011).

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