26979 Probiotic OY15 larval survival counts Data Set Published / External 23140 Aquaculture Sustainability Branch (ASB) Project Completed 2011 Environmentally-friendly methods for controlling microbial pathogenesis in aquaculture with probiotic bacteria are becoming increasingly preferred over use of chemical means, such as disinfectants or antibiotics. Recent research at the Milford Laboratory has shown that naturally-occurring bacteria isolated from the digestive glands of adult oysters, Crassostrea virginica, may be used as potential probiotic candidates in oyster larviculture. Probiotic candidate selection was based upon the Kirby Bauer Disc Diffusion Method, in which zones of inhibition suggested competitive exclusion of a known shellfish larvae pathogen (B183). In challenge studies, survival of 2-day old oyster larvae supplemented with probiotic candidate OY15 (Vibrio sp.) was similar (p<0.3883) to that of control larvae (no added bacteria), indicating no harmful effects. Further, addition of OY15 to oyster larvae challenged with B183 significantly improved survival (p<0.0141) compared to the pathogen alone. An in vitro study to determine the effects of OY15 or the pathogen B183 upon oyster hemocytes and their immune functions demonstrated immuno-stimulation of oyster hemocytes by OY15 and immune-suppression by B183, suggesting an immuno-modulation mechanism for OY15. Environmentally-friendly methods for controlling microbial pathogenesis in aquaculture with probiotic bacteria are becoming increasingly preferred over use of chemical means, such as disinfectants or antibiotics. Recent research at the Milford Laboratory has shown that naturally-occurring bacteria isolated from the digestive glands of adult oysters, Crassostrea virginica, may be used as potential probiotic candidates in oyster larviculture. ISOLATION AND EVALUATION OF NEW PROBIOTIC BACTERIA FOR USE IN SHELLFISH HATCHERIES: I. ISOLATION AND SCREENING FOR BIOACTIVITY. H. J. LIM, D. KAPAREIKO, E. J. SCHOTT, A. HANIF AND G. H. WIKFORS. Journal of Shellfish Research, Vol. 30, No. 3, 609–615, 2011. Theme Crassostrea virginica Theme Vibrio Theme disease prevention Theme larvae survival Theme oyster larviculture Theme probiotic bacteria Theme shellfish larvae Temporal spring Spatial Northeastern United States Stratum Shellfish hatcheries NEFSC Milford Lab Milford CT USA Data Set MS Excel Spreadsheet As Needed Table (digital) None None Not applicable 32756 Week[x] buckets [date] Published / External Completed Oyster larvae counts and survival and mortality data. There are multiple worksheets in this format named: Wk1 buckets 3-15-2008 Wk1 buckets 3-17-2008 Wk1 buckets 3-19-2008 Wk2 buckets 3-21-2008 Wk2 buckets 3-24-2008 Wk2 buckets 3-26-2008 Wk3 buckets 3-28-2008 Loaded by batch 8836, 05-18-2016 08:16 Spreadsheet Yes Oyster larvae counts and survival and mortality data. There are multiple worksheets in this format named: Wk1 buckets 3-15-2008 Wk1 buckets 3-17-2008 Wk1 buckets 3-19-2008 Wk2 buckets 3-21-2008 Wk2 buckets 3-24-2008 Wk2 buckets 3-26-2008 Wk3 buckets 3-28-2008 1 Treatment CHARACTER 20 Yes Yes Active treatment used during this larval bioassay: no-bacteria control, pathogen added, probiotic added, combination pathogen and probiotic. CHARACTER 2 Dosage CHARACTER 20 Yes Yes Active Bacterial dosages used for each treatment. CHARACTER 3 Bucket NUMBER 1 Yes Yes 1 0 Active Bucket number. Four replicate buckets/ treatment. 4 treatments. NUMBER 4 # Live NUMBER 3 Yes Yes 3 0 Active Actual counts of live oyster larvae/treatment. NUMBER 5 # Dead NUMBER 3 Yes Yes 3 0 Active Actual counts of dead oyster larvae/treatment. NUMBER 6 Total NUMBER 3 Yes Yes 3 0 Active Total # of larvae counted. NUMBER 7 % Survival Bucket NUMBER 6 Yes Yes 5 2 Active Percent survival calculation. (Mean live/Mean total)x100 NUMBER 8 % Mortality NUMBER 6 Yes Yes 5 2 Active Percent mortality calculation. (100 - % Survival) NUMBER 9 Live in 8000 mls NUMBER 8 Yes No 7 2 Active Calculation: # Live x 250 mls NUMBER 10 % Survival Initial NUMBER 6 Yes No 5 2 Active Calculation: (# Live/80000) x 100 NUMBER 32757 Wk3 final 3-28-2008 Published / External Completed Oyster larvae counts and survival and mortality data Loaded by batch 8836, 05-18-2016 08:16 Spreadsheet Yes Oyster larvae counts and survival and mortality data 1 Date DATE 9 Yes Yes Active Date of larval sampling for counts. DATE 2 Treatment CHARACTER 32 Yes Yes Active Bacterial dosages used for each treatment. CHARACTER 3 TRT NUMBER 1 Yes Yes 1 0 Active Treatment code number. NUMBER 4 Days NUMBER 2 Yes Yes 2 0 Active Days into experiment. NUMBER 5 PctSvlbucket NUMBER 6 Yes No 5 2 Active Percent Survival of larvae in buckets. NUMBER 6 PercentSurvinitial NUMBER 6 Yes No 5 2 Active Percent Survival of larvae initially. NUMBER 7 Freqbucket NUMBER 4 Yes No 3 2 Active Percent survival in buckets calculation to frequency. (E3/100). NUMBER 8 Freqiniti NUMBER 4 Yes No 3 2 Active Percent survival initial calculation to frequency. (F3/100). NUMBER 9 SqrtfreqB NUMBER 6 Yes No 5 4 Active Transformation Calculation: (=SQRT (G3)) NUMBER 10 Sqrtfreql NUMBER 6 Yes No 5 4 Active Transformation Calculation: (=SQRT (H3)) NUMBER 11 Arcsinbucket NUMBER 8 Yes No 7 6 Active Transformation Calculation: (=ASIN (I3)) NUMBER 12 Arcsininitial NUMBER 8 Yes No 7 6 Active Transformation Calculation: (=ASIN (J3)) NUMBER 13 Path NUMBER 1 Yes No 1 0 Active Grouping Labels for pathogen NUMBER 14 Probiotic NUMBER 1 Yes No 1 0 Active Grouping Labels for probiotic NUMBER 32758 Wk3 stats 3-28-2008 Published / External Completed Oyster larvae counts and survival and mortality data Loaded by batch 8836, 05-18-2016 08:16 Spreadsheet Yes Oyster larvae counts and survival and mortality data 1 Treatment CHARACTER 32 Yes Yes Active treatment used during this larval bioassay: no-bacteria control, pathogen added, probiotic added, combination pathogen and probiotic. CHARACTER 2 TRT NUMBER 1 Yes Yes 1 0 Active Treatment code number. NUMBER 3 Days NUMBER 2 Yes Yes 2 0 Active Days into experiment. NUMBER 4 PctSvlbucket NUMBER 6 Yes Yes 5 2 Active Percent Survival in each bucket. NUMBER 5 Ave % live NUMBER 6 Yes No 5 2 Active Mean % live larvae. NUMBER 6 Error % live NUMBER 6 Yes No 5 2 Active Standard error % live. NUMBER Data Steward 2011 Person Kapareiko, Diane N diane.kapareiko@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 USA 203-882-6539 203-882-6570 Data Steward 2011 Person Wikfors, Gary H gary.wikfors@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 USA 203-882-6525 203-882-6570 Distributor 2011 Organization Northeast Fisheries Science Center NEFSC https://ror.org/02nc0ck44 166 Water Street Woods Hole MA 02543-1026 USA (508)495-2000 https://www.fisheries.noaa.gov/about/northeast-fisheries-science-center NEFSC Home Page Online Resource 8:00 a.m. to 4:30 p.m. Monday - Friday Metadata Contact 2015 Person Kapareiko, Diane N diane.kapareiko@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 USA 203-882-6539 203-882-6570 Metadata Contact 2015 Person Wikfors, Gary H gary.wikfors@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 USA 203-882-6525 203-882-6570 Originator 2011 Person Kapareiko, Diane N diane.kapareiko@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 USA 203-882-6539 203-882-6570 Originator 2011 Person Wikfors, Gary H gary.wikfors@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 USA 203-882-6525 203-882-6570 Point of Contact 2011 Person Wikfors, Gary H gary.wikfors@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 USA 203-882-6525 203-882-6570 Point of Contact 2011 Person Kapareiko, Diane N diane.kapareiko@noaa.gov 212 Rogers Avenue Milford CT 06460-6499 USA 203-882-6539 203-882-6570 -73.053572 -73.053572 41.212161 41.212161 Oysters cultured, bled or spawned occurred at the Milford Laboratory with the above latitude and longitude. Range 2006 2011 Bacterial culturing of digestive glands of oysters began 2006 resulting in 2011 publications. Unclassified See Data Access Constraints NEFSC Data Access Procedure: 1. Formal request in writing usually to the data owner/contact or Center Director; 2. Requester is contacted by data owner to review and verify the request content and details for data delivery options. 3. If data is confidential then owner will determine if the data may be released to the requester; 4. If data can be released, the data is downloaded and packaged for delivery electronically; or the requester may be directed to where the data is available online. The Milford laboratory is currently involved in a CRADA (Cooperative Research and Development Agreement) with Envera LLC, to mass-culture Milford probiotic strain OY15 and make freeze-dried or spray-dried formulations. This may cause a constraint on access to the data as intellectual property, until the CRADA has been completed (June 2016) and agreed upon with collaborators. NEFSC Data Access Constraint: If data is stored on an internal NEFSC network, the requester may not have direct access to query the data unless they are also authorized for network access. http://www.bioone.org/doi/full/10.2983/035.030.0303 Online Resource PDF ISOLATION AND EVALUATION OF NEW PROBIOTIC BACTERIA FOR USE IN SHELLFISH HATCHERIES: I. ISOLATION AND SCREENING FOR BIOACTIVITY. Journal of Shellfish Research, Vol. 30, No. 3, 609–615, 2011 http://www.bioone.org/doi/full/10.2983/035.030.0304 Online Resource PDF ISOLATION AND EVALUATION OF NEW PROBIOTIC BACTERIA FOR USE IN SHELLFISH HATCHERIES: II. EFFECTS OF A VIBRIO SP. PROBIOTIC CANDIDATE UPON SURVIVAL OF OYSTER LARVAE (CRASSOSTREA VIRGINICA) IN PILOT-SCALE TRIALS. Journal of Shellfish Research, Vol. 30, No. 3, 617–625, 2011. Microsoft Excel 2010, Windows 7 Professional, summer 2013 oyster larvae challenges.xls, 33kb Larval counts are performed on a random 1ml subsample raken from a well-mixed, 250ml concentrated volume of larvae in seawater. On rare occasion, a count may be missing from the database due to accidental loss of sample. Data validation during data entry Yes Unknown Yes Yes 6 months No Archiving Intended Unknown Regularly-scheduled desktop and network backups for disaster recovery and contingency planning. Fisher's Island oysters spawned at the Milford Laboratory, and larval bioassays run with 4 treatments: no bacteria control, probiotic OY15, pathogen B183 (Vibrio Corallilyticus) and combination treatment with OY15+B183. Bacteria were dosed every other day and larvae subsamples were taken for survival counts. 32756 Entity 32757 Entity 32758 Entity gov.noaa.nmfs.inport:26979 Patricia Jones 2015-09-16T10:19:53 SysAdmin InPortAdmin 2026-03-04T19:17:17 2016-06-28 Northeast Fisheries Science Center NEFSC 166 Water Street Woods Hole MA 02543-1026 USA (508)495-2000 https://www.nefsc.noaa.gov 8:00 a.m. to 4:30 p.m. Monday - Friday 1001 Public No No 2016-06-28 1 Year 2017-06-28