28062
AFSC/RACE/FBEP/Laurel: Role of temperature on lipid/fatty acid composition in Pacific cod (Gadus macrocephalus) eggs and unfed larvae
AFSC/RACE/FBEP/Laurel: Role of temperature on lipid/fatty acid composition in Pacific cod (Gadus macrocephalus) eggs and unfed larvae
Data Set
Published / External
22355
Fisheries Behavioral Ecology Program
Project
Completed
2012
This dataset is from a laboratory experiment examining whether marine fish embryos physiologically adapt to changing temperature environments.
The objectives of the study that produced this dataset were to evaluate temperature effects on changes in the lipids and fatty acids of developing embryos of Pacific cod.
Loaded by FGDC Metadata Uploader, batch 7460, 10-29-2015 11:57
The following FGDC sections are not currently supported in InPort, but were preserved and will be included in the FGDC export:
- Taxonomy (FGDC:taxonomy)
- Spatial Reference Information (FGDC:spref),
Theme
Pacific cod
Theme
eggs
Theme
fatty acids
Theme
larvae
Theme
lipids
Theme
temperature
Alaska Fisheries Science Center
Seattle
WA
USA
Data Set
MS Excel Spreadsheet
None Planned
Table (digital)
http://link.springer.com/article/10.1007/s00227-012-1989-3/fulltext.html
The user is responsible for the results of any application of this data for other than its intended purpose. NOAA denies liability if the data are misused.
The project that produced this dataset was supported in part with funding from the North Pacific Research Board (NPRB) grant #R0605.
Iatroscan
Not applicable
Not applicable
28333
Temp effects_lipids_fattyacids_Pcod eggs_Laurel et al 2012
Temp effects_lipids_fattyacids_Pcod eggs_Laurel et al 2012
Published / External
Completed
Data from a laboratory experiment that examined the role of temperature on lipid/fatty acid composition in Pacific cod eggs and unfed larvae.
Loaded by FGDC Metadata Uploader, batch 7460, 10-29-2015 11:57
Spreadsheet
Data from a laboratory experiment that examined the role of temperature on lipid/fatty acid composition in Pacific cod eggs and unfed larvae.
1
Sample
NUMBER
No
No
Active
Sample number
1 - 92
2
Date
NUMBER
No
No
Active
Date sample was processed
4/12/10 - 5/23/10
3
Day
NUMBER
No
No
Active
Day of sampling (days post fertilization)
days
1 - 42
4
Temp
NUMBER
No
No
Active
Temperature treatment
degrees Celsius
0 - 8
5
Type
Unknown
No
No
Active
Embryo stage
Egg or Larvae
6
Tank #
NUMBER
No
No
Active
Laboratory identification of sampled tank
1 - 15
7
14:0
NUMBER
No
No
Active
Percent 14:0
0.64 - 1.82
8
Trimethyltridecanoic acid
NUMBER
No
No
Active
Percent trimethyl tridecanoic acid
0 - 0.21
9
14:1
NUMBER
No
No
Active
Percent 14:1
0 - 0.06
10
15:0i
NUMBER
No
No
Active
Percent 15:0i
0 - 0.33
11
15:0ai
NUMBER
No
No
Active
Percent 15:0ai
0 - 0.28
12
15:0
NUMBER
No
No
Active
Percent 15:0
0 - 0.43
13
15:1
NUMBER
No
No
Active
Percent 15:1
0 - 0.11
14
16:0i
NUMBER
No
No
Active
Percent 16:0i
0 - 0.76
15
16:0ai
NUMBER
No
No
Active
Percent 16:0ai
0
16
pristanic acid
NUMBER
No
No
Active
Percent pristanic acid
0 - 1.34
17
16:0
NUMBER
No
No
Active
Percent 16:0
16.61 - 25.73
18
16:1w11
NUMBER
No
No
Active
Percent 16:1w11
0 - 0.13
19
16:1w9
NUMBER
No
No
Active
Percent 16:1w9
0 - 1.91
20
16:1w7
NUMBER
No
No
Active
Percent 16:1w7
0 - 5.74
21
16:1w5
NUMBER
No
No
Active
Percent 16:1w5
0.16 - 0.34
22
17:0i
NUMBER
No
No
Active
Percent 17:0i
0 - 0.61
23
17:0ai
NUMBER
No
No
Active
Percent 17:0ai
0 - 0.35
24
16:2w4
NUMBER
No
No
Active
Percent 16:2w4
0 - 1.11
25
phytanic acid
NUMBER
No
No
Active
Percent phytanic acid
0 - 0.88
26
17:0
NUMBER
No
No
Active
Percent 17:0
0 - 0.32
27
16:3w4
NUMBER
No
No
Active
Percent 16:3w4
0 - 0.52
28
17:2w4
NUMBER
No
No
Active
Percent 17:2w4
0 - 3.63
29
16:4w3
NUMBER
No
No
Active
Percent 16:4w3
0 - 0.51
30
16:4w1
NUMBER
No
No
Active
Percent 16:4w1
0 - 0.97
31
18:0
NUMBER
No
No
Active
Percent 18:0
0 - 5.43
32
18:1w11
NUMBER
No
No
Active
Percent 18:1w11
0 - 12.31
33
18:1w9
NUMBER
No
No
Active
Percent 18:1w9
6.26 - 16.34
34
18:1w7
NUMBER
No
No
Active
Percent 18:1w7
0 - 8.86
35
18:1w6
NUMBER
No
No
Active
Percent 18:1w6
0 - 0.23
36
18:1w5
NUMBER
No
No
Active
Percent 18:1w5
0 - 0.46
37
18:2a
NUMBER
No
No
Active
Percent 18:2
0 - 0.09
38
18:2b
NUMBER
No
No
Active
Percent 18:2
0 - 0.37
39
18:2w6
NUMBER
No
No
Active
Percent 18:2w6
0 - 2.35
40
18:2w4
NUMBER
No
No
Active
Percent 18:2w4
0 - 0.28
41
18:3w6
NUMBER
No
No
Active
Percent 18:3w6
0 - 0.15
42
19:0
NUMBER
No
No
Active
Percent 19:0
0
43
18:3w4
NUMBER
No
No
Active
Percent 18:3w4
0 - 0.09
44
18:3w3
NUMBER
No
No
Active
Percent 18:3w3
0 - 0.48
45
18:4w3
NUMBER
No
No
Active
Percent 18:4w3
0 - 0.51
46
18:4w1
NUMBER
No
No
Active
Percent 18:4w1
0 - 0.22
47
20:0
NUMBER
No
No
Active
Percent 20:0
0 - 0.17
48
18:5w3
NUMBER
No
No
Active
Percent 18:5w3
0 - 0.32
49
20:1w11
NUMBER
No
No
Active
Percent 20:1w11
0 - 1.42
50
20:1w9
NUMBER
No
No
Active
Percent 20:1w9
0 - 1.73
51
20:1w7
NUMBER
No
No
Active
Percent 20:1w7
0 - 0.30
52
20:2a
NUMBER
No
No
Active
Percent 20:2a
0 - 0.18
53
20:2b
NUMBER
No
No
Active
Percent 20:2b
0 - 0.34
54
20:2w6
NUMBER
No
No
Active
Percent 20:2w6
0 - 0.37
55
20:3w6
NUMBER
No
No
Active
20:3w6
0 - 0.83
56
21:0
NUMBER
No
No
Active
Percent 21:0
0
57
20:4w6
NUMBER
No
No
Active
Percent 20:4w6
1.13 - 2.98
58
20:3w3
NUMBER
No
No
Active
Percent 20:3w3
0
59
20:4w3
NUMBER
No
No
Active
Percent 20:4w3
0 - 0.69
60
20:5w3
NUMBER
No
No
Active
Percent 20:5w3
8.66 - 19.63
61
22:0
NUMBER
No
No
Active
Percent 22:0
0 - 0.19
62
22:1w11(13)
NUMBER
No
No
Active
Percent 22:1w11(13)
0 - 0.57
63
22:1w9
NUMBER
No
No
Active
Percent 22:1w9
0 - 0.54
64
22:1w7
NUMBER
No
No
Active
Percent 22:1w7
0 - 0.08
65
22:2a
NUMBER
No
No
Active
Percent 22:2a
0 - 1.49
66
22:2b
NUMBER
No
No
Active
Percent 22:2b
0 - 1.85
67
21:5w3
NUMBER
No
No
Active
Percent 21:5w3
0 - 0.62
68
23:0
NUMBER
No
No
Active
Percent 23:0
0
69
22:4w6
NUMBER
No
No
Active
Percent 22:4w6
0 - 0.20
70
22:5w6
NUMBER
No
No
Active
Percent 22:5w6
0 - 0.26
71
22:4w3
NUMBER
No
No
Active
Percent 22:4w3
0 - 1.70
72
22:5w3
NUMBER
No
No
Active
Percent 22:5w3
0 - 2.03
73
24:0
NUMBER
No
No
Active
Percent 24:0
0 - 0.10
74
22:6w3
NUMBER
No
No
Active
Percent 22:6w3
9.64 - 32.13
75
24:1
NUMBER
No
No
Active
Percent 24:1
0 - 2.67
76
Total
NUMBER
No
No
Active
Percent total
100
77
DHA:EPA
NUMBER
No
No
Active
Ratio of DHA to EPA
1.11 - 2.12
78
µg per individual
Unknown
No
No
Active
Embryo stage and sample/replicate number for micrograms per embryo, amount per embryo dry weight, and percentage calculations
79
Hydrocarbons1
NUMBER
No
No
Active
Hydrocarbon content
micrograms per embryo
0.02 - 0.31
80
Triacylglycerols1
NUMBER
No
No
Active
Triacylglycerol content
micrograms per embryo
0.01 - 1.31
81
Free Fatty Acids1
NUMBER
No
No
Active
Free fatty acid content
micrograms per embryo
0 - 0.09
82
Alcohols
NUMBER
No
No
Active
Alcohol content
micrograms per embryo
0
83
Sterols1
NUMBER
No
No
Active
Sterol content
micrograms per embryo
0.16 - 1.22
84
Diacylglycerols
NUMBER
No
No
Active
Diacylglycerol content
micrograms per embryo
0
85
Acetone Mobile Polar Lipids1
NUMBER
No
No
Active
Acetone mobile polar lipid content
micrograms per embryo
0 - 0.64
86
Phospholipids1
NUMBER
No
No
Active
Phospholipid content
micrograms per embryo
0.56 - 7.20
87
Total Lipids
NUMBER
No
No
Active
Total lipid content
micrograms per embryo
0.86 - 10.09
88
Hydrocarbons2
NUMBER
No
No
Active
Hydrocarbon amount per embryo dry weight
0.12 - 2.74
89
Triacylglycerols2
NUMBER
No
No
Active
Triacylglycerols amount per embryo dry weight
0.06 - 12.33
90
Free Fatty Acids2
NUMBER
No
No
Active
Free fatty acid amount per embryo dry weight
0 - 0.68
91
Sterols2
NUMBER
No
No
Active
Sterol amount per embryo dry weight
0.53 - 11.90
92
Acetone Mobile Polar Lipids2
NUMBER
No
No
Active
Acetone mobile polar lipid content per embryo dry weight
0 - 5.84
93
Phospholipids2
NUMBER
No
No
Active
Phospholipid amount per embryo dry weight
3.95 - 67.27
94
Total Lipids2
NUMBER
No
No
Active
Total lipid amount per embryo dry weight
4.99 - 92.50
95
Hydrocarbons3
NUMBER
No
No
Active
Percent hydrocarbons
0.65 - 9.78
96
Triacylglycerols3
NUMBER
No
No
Active
Percent triacylglycerols
0.57 - 15.34
97
Free Fatty Acids3
NUMBER
No
No
Active
Percent free fatty acids
0 - 2.02
98
Sterols3
NUMBER
No
No
Active
Percent sterols
8.32 - 20.46
99
Acetone Mobile Polar Lipids3
NUMBER
No
No
Active
Percent acetone mobile polar lipids
0 - 13.54
100
Phospholipids3
NUMBER
No
No
Active
Percent phospholipids
54.84 - 84.19
Data Steward
2015-10-29
Person
Spencer, Mara
mara.spencer@noaa.gov
Distributor
2015-10-29
Person
Laurel, Ben
ben.laurel@noaa.gov
Metadata Contact
2015-10-29
Person
Spencer, Mara
mara.spencer@noaa.gov
Originator
2015-10-29
Person
Laurel, Ben
ben.laurel@noaa.gov
Point of Contact
2015-10-29
Person
Laurel, Ben
ben.laurel@noaa.gov
observed
-124.043955
-124.043955
44.621316
44.621316
Hatfield Marine Science Center, Newport, Oregon
Discrete
2006
Unclassified
Unknown
No restriction for accessing this dataset
Must cite originator if used in publications, reports, presentations, etc., and must understand metadata prior to use.
None
None
https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/FBEP/Laurel%3B%20Role%20of%20temperature%20on%20lipid%2Bfatty%20acid%20composition%20in%20Pacific%20cod%20%28Gadus%20macrocephalus%29%20eggs%20and%20unfed%20larvae.csv
Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/15/2021. Contact: Daniel.woodrich@noaa.gov
http://link.springer.com/article/10.1007/s00227-012-1989-3/fulltext.html
Online Resource
HTML
Microsoft Excel
See Laurel, B.J., Copeman, L.A., Parrish, C.C., 2012. Role of temperature on lipid/fatty acid composition in Pacific cod (Gadus macrocephalus) eggs and unfed larvae. Marine Biology. 159: 2025-2034
Not applicable
Not applicable
Data was checked for outliers.
No
Unknown
No
No
Yes
Unknown
No delay
NCEI-MD
Unknown
IT Security and Contingency Plan for the system establishes procedures and applies to the functions, operations, and resources necessary to recover and restore data as hosted in the Western Regional Support Center in Seattle, Washington, following a disruption.
Adult Pacific cod were collected by commercial jigging vessels in Chiniak Bay, Kodiak, AK during the spawning season in March 2006. Gametes were fertilized in the field from separate parents (1 female, 3 males) and shipped to the Hatfield Marine Science Center in Newport, OR.
Eggs and larvae were repeatedly sampled from incubation trays at different developmental stages. A total of 100 individual eggs or larvae was collected for each sampling period and pooled for each lipid/FA analysis.
Lipid classes were determined using thin layer chromatography with flame ionization detection. The absolute amount of FA per larvae was determined using conversion factors described by Budge (1999) in which the glycerol, phosphate, and other functional groups are subtracted from the acyl lipid class mass in order to obtain the mass of fatty acids per lipid class.
Role of temperature on lipid/fatty acid composition in Pacific cod (Gadus macrocephalus) eggs and unfed larvae
Marine Biology
http://link.springer.com/article/10.1007/s00227-012-1989-3/fulltext.html
1
Egg collection and incubation
Role of temperature on lipid/fatty acid composition in Pacific cod (Gadus macrocephalus) eggs and unfed larvae
2
Sampling
Role of temperature on lipid/fatty acid composition in Pacific cod (Gadus macrocephalus) eggs and unfed larvae
3
Lipid and fatty acid analysis
Role of temperature on lipid/fatty acid composition in Pacific cod (Gadus macrocephalus) eggs and unfed larvae
4
Data analysis
Role of temperature on lipid/fatty acid composition in Pacific cod (Gadus macrocephalus) eggs and unfed larvae
28333
Entity
Temp effects_lipids_fattyacids_Pcod eggs_Laurel et al 2012
gov.noaa.nmfs.inport:28062
Nancy Roberson
2015-10-19T12:06:06
SysAdmin InPortAdmin
2022-08-09T17:11:13
2016-05-18
Alaska Fisheries Science Center
AFSC
7600 Sand Point Way N.E., Building 4
Seattle
WA
98115
USA
http://www.afsc.noaa.gov
0700-1700 Pacific Time
1001
Public
No
2016-05-18
1 Year
2017-05-18
None
fish
vertebrates
Pacific cod
Gadus macrocephalus
Kingdom
Animalia
animals
Subkingdom
Bilateria
Infrakingdom
Deuterostomia
Phylum
Chordata
chordates
Subphylum
Vertebrata
vertebrates
Infraphylum
Gnathostomata
Superclass
Osteichthyes
bony fishes
Class
Actinopterygii
ray-finned fishes
spiny rayed fishes
Subclass
Neopterygii
neopterygians
Infraclass
Teleostei
Superorder
Paracanthopterygii
Order
Gadiformes
Family
Gadidae
cods
Subfamily
Gadinae
cods
haddock
Genus
Gadus
common codfishes
Species
Gadus macrocephalus
Pacific cod
meters
Temp effects_lipids_fattyacids_Pcod eggs_Laurel et al 2012
Data from a laboratory experiment that examined the role of temperature on lipid/fatty acid composition in Pacific cod eggs and unfed larvae.
Dataset developers
% time to hatch
Percent development time calculated as the day of sampling (days post fertilization) divided by the days to the last hatching larva
Dataset developers
12.5
100
percent
Temperature1
Treatment temperature
Dataset developers
0
8
degrees Celsius
TAG (micrograms)
Triacyglycerol content
Dataset developers
0.01
1.31
micrograms
PL (micrograms)
Phospholipid content
Dataset developers
0.56
7.20
micrograms
ST (micrograms)
Sterol content
Dataset developers
0.16
1.22
micrograms
Total lipid (micrograms)
Total lipid content
Dataset developers
0.86
10.09
micrograms
Temperature2
Temperature treatment
Dataset developers
0
8
degrees Celsius
% Phospholipid
Change in % composition of phospholipid from the early egg stage (20-25% development) to the newly hatched larval stage
Dataset developers
82.91
99.82
percent
%TAG
Percent triacyglycerols
Dataset developers
0.18
17.09
percent
Temperature3
Temperature treatment
Dataset developers
0
8
degrees Celsius
18:1n-9
Oleic acid content
Dataset developers
0.59
1.86
micrograms per embryo
18:2n-6
Linoleic acid content
Dataset developers
0.55
0.66
micrograms per embryo
20:1n-9
Eicosenoic acid content
Dataset developers
0.62
2.21
micrograms per embryo
AA
Arachidonic acid content
Dataset developers
0.59
2.19
micrograms per embryo
EPA
Eicosapentaenoic acid content
Dataset developers
0.61
2.19
micrograms per embryo
DHA
Docosahexaenoic acid content
Dataset developers
0.59
2.26
micrograms per embryo
Total FA
Total fatty acid content
Dataset developers
0.59
1.44
micrograms per embryo
Total SFA
Total saturated fatty acid content
Dataset developers
1.47
3.58
micrograms per embryo
Total MUFA
Total monounsaturated fatty acid content
Dataset developers
1.47
3.58
micrograms per embryo
Total PUFA
Total polyunsaturated fatty acid content
Dataset developers
1.20
2.92
micrograms per embryo
Total n-3
Total omega-3 fatty acid content
Dataset developers
2.05
5.00
micrograms per embryo
Temperature4
Temperature treatment
Dataset developers
0
8
degrees Celsius
Days post fertilization1
Embryo age
Dataset developers
1
42
days post fertilization
DHA:EPA ratio
Ratio of docosahexaenoic acid to eicosapentaenoic acid
Dataset developers
1.11
2.12
Temperature5
Temperature treatment
Dataset developers
0
8
degrees Celsius
Days post fertilization2
Embryo age
Dataset developers
1
42
days post fertilization
%AA
Percent arachidonic acid
Dataset developers
1.13
2.98
percent
PUFA:SFA ratio
Ratio of polyunsaturated to saturated fatty acids in newly hatched Pacific cod
Dataset developers
2.10
2.31
Standard error
Standard error of ratio of polyunsaturated to saturated fatty acids
Dataset developers
0.00
0.06
Temperature6
Temperature treatment
Dataset developers
0
8
degrees Celsius