28298
AFSC/RACE/EcoFOCI - Walleye Pollock larvae condition data measured in support of EcoFOCI assessment surveys and ecosystem observations in the southeastern Bering Sea and western Gulf of Alaska from 2007 to 2014
AFSC/RACE/EcoFOCI - Walleye Pollock larvae condition data in the southeastern Bering Sea and western Gulf of Alaska 2007 - 2014
Data Set
Published / External
22352
Recruitment Processes Program
Project
In Work
The datasets contain larval size, water temperature, cell cycle data, and classification of larval condition.
The datasets were developed to assess the condition of Walleye Pollock larvae using larval size, water temperature, and cell cycle data measured with flow cytometry.
Supplemental Information - Data Parameters and Units - Descriptive Information about the Data Parameters and Units.
The datasets contain larval standard length measured in mm, the proportion of cells in the S and G2/M phases of the cell cycle, and the ratio of the number S phase nuclei to the number of G1 phase nuclei with high nRNA content measured with flow cytometry. Also included is the mean water temperature (°C) between the surface and 40 m depth.
Supplemental Information – Methods - Descriptive Information about the methods used.
Larvae were collected using a 60 cm bongo frame fitted with 0.505 mm mesh nets and were frozen at -80°C. Tissue preparation, flow cytometry protocol, and data analysis are described in Porter and Bailey (2011 and 2013).
Supplemental Information – Instruments - Descriptive Information about the instruments and equipment used.
A BD Biosciences Influx flow cytometer (BD Biosciences, San Jose, CA) was used to analyze each larva. The proportion of nuclei in the S, and G2/M phases was calculated for each larva with MultiCycle AV software, vers. 4.0 (Phoenix Flow Systems, San Diego, CA). FCS Express flow cytometry analysis software, vers. 3.0 (De Novo Software, Los Angeles, CA) was used to calculate the ratio of the number S phase nuclei to the number of G1 phase nuclei with high nRNA content. A detailed description of the analyses is given in Porter and Bailey 2013.
Supplemental Information - Sampling Scales and Rates - Descriptive Information about the sampling spatial and temporal scales and rates.
Samples were taken from the southeastern Bering Sea and western Gulf of Alaska, and were conducted opportunistically over multiple years.
Supplemental Information - Error Analysis - Descriptive Information about the error analysis.
Supplemental Information - Provenance and Historical References -
Descriptive Information about the provenance, historical data, key information packaged elsewhere.
PORTER, S.M., and K.M. BAILEY. 2011. Assessing the condition of walleye pollock Theragra chalcogramma (Pallas) larvae using muscle-based flow cytometric cell cycle analysis. J. Exp. Mar. Biol. Ecol. 399:101-109.
PORTER, S. M., and K. M. BAILEY. 2013. Using measurements of muscle cell nuclear
RNA with flow cytometry to improve assessment of larval condition of walleye
pollock (Gadus chalcogrammus). Fish. Bull., U.S. 111:337-351.
Theme
condition
Theme
fish larvae
Spatial
Bering Sea
Spatial
Gulf of Alaska
Alaska Fisheries Science Center
Seattle
WA
USA
Data Set
CSV Files
Irregular
Document (digital)
BD Biosciences Influx flow cytometer
N/A
60cm Bongo net
Data Steward
2015
Person
Porter, Steve
steve.porter@noaa.gov
Distributor
2015
Person
Porter, Steve
steve.porter@noaa.gov
Metadata Contact
2019
Person
Bahl, Kimberly
kimberly.bahl@noaa.gov
206 526 4314
Originator
2015
Person
Porter, Steve
steve.porter@noaa.gov
Point of Contact
2015
Person
Porter, Steve
steve.porter@noaa.gov
Ground Condition
-180
-150
65
50
Range
2007
2014
Unclassified
N/A
N/A
Contact data steward
Contact point of contact.
Contact point of contact.
None
None
https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/RPP/2007%20walleye%20pollock%20larvae%20condition.csv
2015
Person
Porter, Steve
Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/15/2021. Contact: Daniel.woodrich@noaa.gov
csv (comma-separated values)
CSV - Comma Separated Values (Text)
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/a
N/A
N/A
N/A
N/A
N/A
N/A
PORTER, S. M., and K. M. BAILEY. 2013. Using measurements of muscle cell nuclear
RNA with flow cytometry to improve assessment of larval condition of walleye
pollock (Gadus chalcogrammus). Fish. Bull., U.S. 111:337-351.
No
Unknown
No
No
varies
Data not generated by automated processing.
To Be Determined
varies
local and offsite backupd
A BD Biosciences Influx flow cytometer (BD Biosciences, San Jose, CA) was used to analyze each larva. The proportion of nuclei in the S, and G2/M phases was calculated for each larva with MultiCycle AV software, vers. 4.0 (Phoenix Flow Systems, San Diego, CA). FCS Express flow cytometry analysis software, vers. 3.0 (De Novo Software, Los Angeles, CA) was used to calculate the ratio of the number S phase nuclei to the number of G1 phase nuclei with high nRNA content. A detailed description of the analyses is given in Porter and Bailey 2013.
gov.noaa.nmfs.inport:28298
Tiffany Vance
2015-10-28T12:46:27
SysAdmin InPortAdmin
2023-10-17T16:12:08
2016-05-18
Alaska Fisheries Science Center
AFSC
7600 Sand Point Way N.E., Building 4
Seattle
WA
98115
USA
http://www.afsc.noaa.gov
0700-1700 Pacific Time
1001
Public
No
2016-05-18
1 Year
2017-05-18