28698 Data Set Published / External 2704 HMSRP Hawaiian Monk Seal Health and Disease Metadata Portfolio Project In Work 2018-02-27 Hematology and serum chemistry evaluations are essential to a patients comprehensive health exam by providing measures of organ function, blood cell volume and health status. Reference ranges have to be established to interpret that the values are within normal limits. Presently, hematology and serum chemistry results from healthy, sick and injured Hawaiian monk seals are interpreted using past studies, which were limited by small samples sizes across age classes, collected from captive Hawaiian monk seals or established using frozen samples. This study builds on previous studies in sample size, quality and demographic representation to establish blood reference ranges by using samples collected between 2004 and 2015 from wild monk seals of multiple age classes (n=65 seals; 28 adults, 18 subadults, 8 juveniles, 11 weaned pups). Samples were analyzed by a single veterinary diagnostic laboratory within 24 hours of collection from apparently healthy, free-ranging wild seals sampled opportunistically during population monitoring and research activities. Reference ranges were developed for 50 complete blood cell counts and serum chemistry parameters using the mean of the values within two standard deviations. While there are several possible approaches to establishment of reference ranges, this method was chosen to maintain consistency with those used by the veterinary diagnostic laboratory most commonly used for sample analysis. Key indices evaluated include total white blood cell count (WBC; mean- 8.145 K/uL, SD-1.73 ), red blood cell count (RBC; mean- 3.397 M/uL, SD- 0.46), hematocrit (mean- 51.56%, SD- 4.99), and total serum protein (TP; mean- 7.625g/dL, SD- 0.81). The development of comprehensive hematology and serum chemistry reference ranges for Hawaiian monk seals will enable more consistent and systematic interpretation of results, which will aid in the assessment and treatment of seals in rehabilitation and the determination of health status of seals sampled during translocation and other research/recovery activities. The development of comprehensive hematology and serum chemistry reference ranges for Hawaiian monk seals will enable more consistent and systematic interpretation of results, which will aid in the assessment and treatment of seals in rehabilitation and the determination of health status of seals sampled during translocation and other research/recovery activities. Theme Hawaiian monk seal Theme hematology Pacific Islands Fisheries Science Center Honolulu HI USA Data Set Table (digital) Not applicable Not applicable Not applicable 51658 Published / External Spreadsheet Hawaiian monk seal blood values Atoll NUMBER No No Active Island of Collection Year NUMBER No No Active Year of collection CC_wild TEXT No No Active captive animal or wild animal at time of collection Healthy TEXT No No Active Healthy SEALID TEXT No No Active SEAL ID age_group Number No No Active age group SEALSIZE TEXT No No Active SIZE OF SEAL SEALSEX TEXT No No Active SEAL'S SEX SEALTEMP TEXT No No Active temporary seal ID COLLECDATE DATE No No Active Collection date Number1 NUMBER No No Active Specimen number SUBNUMBER TEXT No No Active Specimen sub number SPECTYPE TEXT No No Active Specimen type Specimen_condition TEXT No No Active Specimen condition Unique_Key TEXT No No Active Unique Key ANALYSIS_DATE DATE No No Active ANALYSIS DATE DIAGLAB TEXT No No Active Diagnostic Lab LABORATORY TEXT No No Active LABORATORY LAB_NUM TEXT No No Active Lab accession number WBC NUMBER No No Active White Blood cell count RBC NUMBER No No Active Red Blood cell count HGB NUMBER No No Active Hemoglobin HCT NUMBER No No Active Hematocrit MCV NUMBER No No Active Mean cell volume MCH NUMBER No No Active Mean corpuscular hemoglobin MCHC NUMBER No No Active mean corpuscular hemoglobin ABSOLUTE_EOSIN NUMBER No No Active Absolute eosinophil count EOSINOPHIL NUMBER No No Active percentage of Eosinophil ABSOLUTE_LYM NUMBER No No Active Absolute lymphosite count LYMPHOCYTES NUMBER No No Active LYMPHOCYTES ABSOLUTE_MONO NUMBER No No Active Absolute monophil count MONOCYTES NUMBER No No Active MONOCYTES NEUT_SEG NUMBER No No Active NEUTROPHIL ABSOLUTE_NEUT NUMBER No No Active Absolute neutrophil count ALK_PHOS NUMBER No No Active alkaline phosphate SGOT_AST NUMBER No No Active Glutamin axaloacetic transaminase SGPT_ALT NUMBER No No Active Glutamic pyruvic transaminase LDH NUMBER No No Active Lactate dehydrogenase GGT NUMBER No No Active y-Glutamyltransferase CPK NUMBER No No Active Creatinine phosphokinase ALBUMIN NUMBER No No Active ALBUMIN GLOBULIN NUMBER No No Active GLOBULIN A_G_RATIO NUMBER No No Active Albumin/Globulin ratio TP NUMBER No No Active total protein BICARBONATE NUMBER No No Active BICARBONATE DIRECT_BILI NUMBER No No Active Direct Bilirubin INDIRECT_BILI NUMBER No No Active Indirect bilirubin TOTAL_BILI NUMBER No No Active Total Bilirubin BUN NUMBER No No Active Blood urea nitrogen CREATININE NUMBER No No Active CREATININE ANION_GAP NUMBER No No Active ANION GAP CALCIUM NUMBER No No Active CALCIUM CHLORIDE NUMBER No No Active CHLORIDE B_C_RATIO NUMBER No No Active Bun/Creatnine ratio CHOLESTEROL NUMBER No No Active CHOLESTEROL GLUCOSE NUMBER No No Active GLUCOSE NA_K_RATIO NUMBER No No Active sodium/potassium ratio PHOSPHORUS NUMBER No No Active PHOSPHORUS POTASSIUM NUMBER No No Active POTASSIUM SODIUM NUMBER No No Active SODIUM REMARKS NUMBER No No Active REMARKS Data Steward 2025-11-11 Person Barbieri, Michelle M michelle.barbieri@noaa.gov 1845 Wasp Blvd. Honolulu HI 96818 USA 808-725-5734 Data Steward 2025-11-11 Person Cedillo, Claudia E claudia.cedillo@noaa.gov Data Steward 2015-11-02 Person Kaufman, Angela C angie.kaufman@noaa.gov 1845 Wasp Blvd. Honolulu HI 96818 USA (808)725-5708 Distributor 2015-12-01 Organization Pacific Islands Fisheries Science Center PIFSC pifsc.info@noaa.gov https://ror.org/02apffz65 1845 Wasp Blvd. Honolulu HI 96818 USA 808-725-5360 https://www.pifsc.noaa.gov Pacific Island Fisheries Science Center homepage Online Resource 8:00 a.m. - 4:30 p.m. Metadata Contact 2025-11-11 Person Khurana, Vikram vikram.khurana@noaa.gov Metadata Contact 2015-11-02 Person Johanos, Thea C thea.johanos-kam@noaa.gov 1845 Wasp Blvd. Honolulu HI 96818 USA (808)725-5709 Originator 2007-10-22 Organization Pacific Islands Fisheries Science Center PIFSC pifsc.info@noaa.gov https://ror.org/02apffz65 1845 Wasp Blvd. Honolulu HI 96818 USA 808-725-5360 https://www.pifsc.noaa.gov Pacific Island Fisheries Science Center homepage Online Resource 8:00 a.m. - 4:30 p.m. Point of Contact 2025-11-11 Person Barbieri, Michelle M michelle.barbieri@noaa.gov 1845 Wasp Blvd. Honolulu HI 96818 USA 808-725-5734 Point of Contact 2007-10-22 Person Kashinsky, Lizabeth lizabeth.kashinsky@noaa.gov 1845 Wasp Blvd. Honolulu HI 96818 USA (808)725-5719 Point of Contact 2007-10-22 Person Kaufman, Angela C angie.kaufman@noaa.gov 1845 Wasp Blvd. Honolulu HI 96818 USA (808)725-5708 Ground Condition -180 -150 30 10 Hawaiian Archipelago, Pacific Region Continuing 1981-12-31 Unclassified NMFS/PIFSC, Protected Species Division, Hawaiian Monk Seal Research Program Data Access, Sharing, and Dissemination Guidelines Requestor will be asked to submit a request to Lizabeth Kashinsky (Lizabeth.Kashinsky@noaa.gov) NMFS/PIFSC, Protected Species Division, Hawaiian Monk Seal Research Program Data Access, Sharing, and Dissemination Guidelines Use of data will ideally be cleared with NMFS/PIFSC, Protected Species Division. https://oceanwatch.pifsc.noaa.gov/xfer/PIFSC_PIRO_bulk_data_download_InPort_28698.tgz 2015-12-01 Organization Pacific Islands Fisheries Science Center https://ror.org/02apffz65 IDEXX_finaldata2015_raw data.csv csv (comma-separated values) CSV - Comma Separated Values (Text) Data are collected both during dedicated efforts and opportunistically at Hawaiian monk seal haulout locations during variable-length field seasons over multiple years. Blood values may vary over time and by animal history. Seal size/sex classes are not equally represented in the data set. Seal size/sex classes are not equally represented in the data set. Standard data life-cycle processes are followed to ensure quality and accuracy. Yes Unknown Yes No 1 year Not applicable NCEI-MD 1 year Data are backed-up daily per PIFSC's Information Technology Services security policies. Statistics used to create a blood reference range for wild, healthy monk seals Blood values from monk seal biological samples Organization Pacific Islands Fisheries Science Center Originator 1 Blood draws were collected during epidemiological studies and opportunistically during taggings and other handling events. Person Barbieri, Michelle M michelle.barbieri@noaa.gov 1845 Wasp Blvd. Honolulu HI 96818 USA 808-725-5734 2 Blood was processed and analyzed for hematology and serum chemistry values. Person Barbieri, Michelle M michelle.barbieri@noaa.gov 1845 Wasp Blvd. Honolulu HI 96818 USA 808-725-5734 51658 Entity gov.noaa.nmfs.inport:28698 Thea Johanos 2015-11-13T19:13:30 SysAdmin InPortAdmin 2025-12-03T01:00:28 2015-12-08 Pacific Islands Fisheries Science Center PIFSC 1845 Wasp Blvd. Honolulu HI 96818 USA 808-725-5300 https://www.pifsc.noaa.gov 8:00 a.m. - 4:30 p.m. 1001 Public No No 2015-12-08 1 Year 2016-12-08