28698
HMSRP Hawaiian Monk Seal Blood Values (Establishing hematology and serum chemistry reference ranges for wild Hawaiian Monk Seals)
Data Set
Published / External
2704
HMSRP Hawaiian Monk Seal Health and Disease Metadata Portfolio
Project
In Work
2018-02-27
Hematology and serum chemistry evaluations are essential to a patients comprehensive health exam by providing measures of organ function, blood cell volume and health status. Reference ranges have to be established to interpret that the values are within normal limits. Presently, hematology and serum chemistry results from healthy, sick and injured Hawaiian monk seals are interpreted using past studies, which were limited by small samples sizes across age classes, collected from captive Hawaiian monk seals or established using frozen samples. This study builds on previous studies in sample size, quality and demographic representation to establish blood reference ranges by using samples collected between 2004 and 2015 from wild monk seals of multiple age classes (n=65 seals; 28 adults, 18 subadults, 8 juveniles, 11 weaned pups). Samples were analyzed by a single veterinary diagnostic laboratory within 24 hours of collection from apparently healthy, free-ranging wild seals sampled opportunistically during population monitoring and research activities. Reference ranges were developed for 50 complete blood cell counts and serum chemistry parameters using the mean of the values within two standard deviations. While there are several possible approaches to establishment of reference ranges, this method was chosen to maintain consistency with those used by the veterinary diagnostic laboratory most commonly used for sample analysis. Key indices evaluated include total white blood cell count (WBC; mean- 8.145 K/uL, SD-1.73 ), red blood cell count (RBC; mean- 3.397 M/uL, SD- 0.46), hematocrit (mean- 51.56%, SD- 4.99), and total serum protein (TP; mean- 7.625g/dL, SD- 0.81). The development of comprehensive hematology and serum chemistry reference ranges for Hawaiian monk seals will enable more consistent and systematic interpretation of results, which will aid in the assessment and treatment of seals in rehabilitation and the determination of health status of seals sampled during translocation and other research/recovery activities.
The development of comprehensive hematology and serum chemistry reference ranges for Hawaiian monk seals will enable more consistent and systematic interpretation of results, which will aid in the assessment and treatment of seals in rehabilitation and the determination of health status of seals sampled during translocation and other research/recovery activities.
Theme
Hawaiian monk seal
Theme
hematology
Pacific Islands Fisheries Science Center
Honolulu
HI
USA
Data Set
Table (digital)
Not applicable
Not applicable
Not applicable
51658
Blood Values
Published / External
Spreadsheet
Hawaiian monk seal blood values
Atoll
NUMBER
No
No
Active
Island of Collection
Year
NUMBER
No
No
Active
Year of collection
CC_wild
TEXT
No
No
Active
captive animal or wild animal at time of collection
Healthy
TEXT
No
No
Active
Healthy
SEALID
TEXT
No
No
Active
SEAL ID
age_group
Number
No
No
Active
age group
SEALSIZE
TEXT
No
No
Active
SIZE OF SEAL
SEALSEX
TEXT
No
No
Active
SEAL'S SEX
SEALTEMP
TEXT
No
No
Active
temporary seal ID
COLLECDATE
DATE
No
No
Active
Collection date
Number1
NUMBER
No
No
Active
Specimen number
SUBNUMBER
TEXT
No
No
Active
Specimen sub number
SPECTYPE
TEXT
No
No
Active
Specimen type
Specimen_condition
TEXT
No
No
Active
Specimen condition
Unique_Key
TEXT
No
No
Active
Unique Key
ANALYSIS_DATE
DATE
No
No
Active
ANALYSIS DATE
DIAGLAB
TEXT
No
No
Active
Diagnostic Lab
LABORATORY
TEXT
No
No
Active
LABORATORY
LAB_NUM
TEXT
No
No
Active
Lab accession number
WBC
NUMBER
No
No
Active
White Blood cell count
RBC
NUMBER
No
No
Active
Red Blood cell count
HGB
NUMBER
No
No
Active
Hemoglobin
HCT
NUMBER
No
No
Active
Hematocrit
MCV
NUMBER
No
No
Active
Mean cell volume
MCH
NUMBER
No
No
Active
Mean corpuscular hemoglobin
MCHC
NUMBER
No
No
Active
mean corpuscular hemoglobin
ABSOLUTE_EOSIN
NUMBER
No
No
Active
Absolute eosinophil count
EOSINOPHIL
NUMBER
No
No
Active
percentage of Eosinophil
ABSOLUTE_LYM
NUMBER
No
No
Active
Absolute lymphosite count
LYMPHOCYTES
NUMBER
No
No
Active
LYMPHOCYTES
ABSOLUTE_MONO
NUMBER
No
No
Active
Absolute monophil count
MONOCYTES
NUMBER
No
No
Active
MONOCYTES
NEUT_SEG
NUMBER
No
No
Active
NEUTROPHIL
ABSOLUTE_NEUT
NUMBER
No
No
Active
Absolute neutrophil count
ALK_PHOS
NUMBER
No
No
Active
alkaline phosphate
SGOT_AST
NUMBER
No
No
Active
Glutamin axaloacetic transaminase
SGPT_ALT
NUMBER
No
No
Active
Glutamic pyruvic transaminase
LDH
NUMBER
No
No
Active
Lactate dehydrogenase
GGT
NUMBER
No
No
Active
y-Glutamyltransferase
CPK
NUMBER
No
No
Active
Creatinine phosphokinase
ALBUMIN
NUMBER
No
No
Active
ALBUMIN
GLOBULIN
NUMBER
No
No
Active
GLOBULIN
A_G_RATIO
NUMBER
No
No
Active
Albumin/Globulin ratio
TP
NUMBER
No
No
Active
total protein
BICARBONATE
NUMBER
No
No
Active
BICARBONATE
DIRECT_BILI
NUMBER
No
No
Active
Direct Bilirubin
INDIRECT_BILI
NUMBER
No
No
Active
Indirect bilirubin
TOTAL_BILI
NUMBER
No
No
Active
Total Bilirubin
BUN
NUMBER
No
No
Active
Blood urea nitrogen
CREATININE
NUMBER
No
No
Active
CREATININE
ANION_GAP
NUMBER
No
No
Active
ANION GAP
CALCIUM
NUMBER
No
No
Active
CALCIUM
CHLORIDE
NUMBER
No
No
Active
CHLORIDE
B_C_RATIO
NUMBER
No
No
Active
Bun/Creatnine ratio
CHOLESTEROL
NUMBER
No
No
Active
CHOLESTEROL
GLUCOSE
NUMBER
No
No
Active
GLUCOSE
NA_K_RATIO
NUMBER
No
No
Active
sodium/potassium ratio
PHOSPHORUS
NUMBER
No
No
Active
PHOSPHORUS
POTASSIUM
NUMBER
No
No
Active
POTASSIUM
SODIUM
NUMBER
No
No
Active
SODIUM
REMARKS
NUMBER
No
No
Active
REMARKS
Data Steward
2015-11-02
Person
Kaufman, Angela C
angie.kaufman@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5708
Distributor
2015-12-01
Organization
Pacific Islands Fisheries Science Center
PIFSC
pifsc.info@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
808-725-5360
https://www.pifsc.noaa.gov
Pacific Island Fisheries Science Center homepage
Online Resource
8:00 a.m. - 4:30 p.m.
Metadata Contact
2015-11-02
Person
Johanos, Thea C
thea.johanos-kam@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5709
Originator
2007-10-22
Organization
Pacific Islands Fisheries Science Center
PIFSC
pifsc.info@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
808-725-5360
https://www.pifsc.noaa.gov
Pacific Island Fisheries Science Center homepage
Online Resource
8:00 a.m. - 4:30 p.m.
Point of Contact
2007-10-22
Person
Kashinsky, Lizabeth
lizabeth.kashinsky@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5719
Point of Contact
2007-10-22
Person
Kaufman, Angela C
angie.kaufman@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5708
Ground Condition
-180
-150
30
10
Hawaiian Archipelago, Pacific Region
Continuing
1981-12-31
Unclassified
NMFS/PIFSC, Protected Species Division, Hawaiian Monk Seal Research Program Data Access, Sharing, and Dissemination Guidelines
Requestor will be asked to submit a request to Lizabeth Kashinsky (Lizabeth.Kashinsky@noaa.gov)
NMFS/PIFSC, Protected Species Division, Hawaiian Monk Seal Research Program Data Access, Sharing, and Dissemination Guidelines
Use of data will ideally be cleared with NMFS/PIFSC, Protected Species Division.
https://oceanwatch.pifsc.noaa.gov/xfer/PIFSC_PIRO_bulk_data_download_InPort_28698.tgz
2015-12-01
Organization
Pacific Islands Fisheries Science Center
IDEXX_finaldata2015_raw data.csv
csv (comma-separated values)
CSV - Comma Separated Values (Text)
Data are collected both during dedicated efforts and opportunistically at Hawaiian monk seal haulout locations during variable-length field seasons over multiple years.
Blood values may vary over time and by animal history. Seal size/sex classes are not equally represented in the data set.
Seal size/sex classes are not equally represented in the data set.
Standard data life-cycle processes are followed to ensure quality and accuracy.
Yes
Unknown
Yes
No
1 year
Not applicable
NCEI-MD
1 year
Data are backed-up daily per PIFSC's Information Technology Services security policies.
Statistics used to create a blood reference range for wild, healthy monk seals
Blood values from monk seal biological samples
Organization
Pacific Islands Fisheries Science Center
Originator
1
Blood draws were collected during epidemiological studies and opportunistically during taggings and other handling events.
Person
Barbieri, Michelle M
michelle.barbieri@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
808-725-5734
2
Blood was processed and analyzed for hematology and serum chemistry values.
Person
Barbieri, Michelle M
michelle.barbieri@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
808-725-5734
51658
Entity
Blood Values
gov.noaa.nmfs.inport:28698
Thea Johanos
2015-11-13T19:13:30
SysAdmin InPortAdmin
2023-11-30T18:11:01
2015-12-08
Pacific Islands Fisheries Science Center
PIFSC
1845 Wasp Blvd.
Honolulu
HI
96818
USA
808-725-5300
http://www.pifsc.noaa.gov
8:00 a.m. - 4:30 p.m.
1001
Public
No
2015-12-08
1 Year
2016-12-08