30880 Data Set Published / External 30657 EFS (Environmental and Fisheries Sciences) Division Project In Work 2018-11-07 Lipophilic shellfish toxins comprise an extensive suite of compounds including those associated with the human syndromes known as diarrhetic shellfish poisoning (DSP) and azaspiracid shellfish poisoning (AZP). As a result of recent bloom events and subsequent human intoxications in Washington State (USA) due to DSP, there is a critical and urgent need for State public health officials to be able to monitor and accurately quantify harmful algal bloom (HAB) species associated with DSP and azaspiracid shellfish poisoning (AZP) and their toxins. There is now evidence that lipophilic toxins associated with DSP and AZP are present in water and/or shellfish, including oysters and mussels from Puget Sound and razor clams from the WA coast.Tight partnership with WDOH, the SoundToxins program, Olympic Region Harmful Algal Blooms (ORHAB) partnership, and Puget Sound shellfish growers (including the Jamestown S’Klallam tribe and other tribal representatives) will facilitate the study of the seasonal variability of lipophilic toxins and toxin-producing species at 10 geographically-distinct sites within Washington State waters where seawater or shellfish have recently been contaminated with these toxins. Stakeholder support throughout the project will ensure the transition of this project to the State at the end of 3 years as we have successfully demonstrated with ORHAB. Implementing routine lipophilic biotoxin monitoring will be a critical first step towards ensuring public safety while also enabling Washington State shellfish growers to sell their product to the European Union once trade is re-established. Contains information regarding toxin producing species and toxin measurements in shellfish and seawater samples. Also includes associated environmental data. Subject to Public Access to Research Results (PARR): Yes Theme Azadinium Theme Azaspiracids Theme Diarrhetic shellfish toxins Theme Dinophysis Theme Tracked Asset Theme biotoxins Theme chemistry Theme fishery-dependent Theme marine toxin Theme toxic effects Spatial NWFSC Montlake Laboratory Spatial Various locations in WA Instrument In-Situ Instrument - In-Situ Instrument (not specified) Instrument Sonde and Probe - Conductivity Instrument Temperature Instrument and Depth Platform Animal and Plant Collection Device Platform Animal and Plant Collection Device - Plankton Collection Device - Plankton Net Platform Water Sampler Bottle - Surface Sample Bottle Northwest Fisheries Science Center Seattle WA USA Data Set Access Database Table (digital) In-Situ Instrument - In-Situ Instrument (not specified), Sonde and Probe - Conductivity, Temperature, and Depth Platform Not Applicable Animal and Plant Collection Device, Animal and Plant Collection Device - Plankton Collection Device - Plankton Net, Water Sampler Bottle - Surface Sample Bottle 54712 AZA Project Seawater Date for Lipophilic Toxins In Washington State 2016 Published / External Planned Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. Spreadsheet Yes PARR Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. 1 SITE TEXT No No Active The name or identification number of a given site at which samples were collected. TEXT As provided 2 DATE DATE No No Active The date (local time) on which as sample was collected. DATE As provided 3 TIME TIME No No Active The time (local time) on the date in which a sample began to be collected. TIME As provided 4 SAMPLEID TEXT No No Active The name or identification number of a individual samples collected at specific sites. IDs incorporate a 2 letter site code followed by the eight digit sampling date and a 2 digit code signifying the sample type. May be followed by an S (surface) or I (3m depth integrated). TEXT As provided 5 LATITUDE NUMBER No No Active The latitude of the sampling site in decmal degrees. Units for values are decimal degrees. NUMBER decimal degrees As provided 6 LONGITUDE NUMBER No No Active The longitude of the sampling site in decimal degrees. Units for values are decimal degrees. NUMBER decimal degrees As provided 7 Other Azadinium or Amphidoma detected TEXT No No Active Indicates whether or not other Azadinium species may be present in a water sample. This result is based on a genus specific quantitative PCR assay that is run if no Azadinium species are detected with species specific quantitative PCR assays. Units for values are Yes or No indicated by a 1 for Yes and a 0 for No. TEXT Yes or No indicated by a 1 for Yes and a 0 for No Blank, 0, or 1 8 A. spinosum (cells/L) TEXT No No Active Enumeration of Azadinum spinosum in a water sample. Units for values are Cells per liter of seawater. TEXT Cells per liter of seawater less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value 9 A. poporum (cells/L) TEXT No No Active Enumeration of Azadinum poporum in a water sample. Units for values are Cells per liter of seawater. TEXT Cells per liter of seawater less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value 10 A. obesum (cells/L) TEXT No No Active Enumeration of Azadinum obesum in a water sample. Units for values are Cells per liter of seawater. TEXT Cells per liter of seawater less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value 54713 AZA Project Shellfish Data for Lipophilic Toxins In Washington State 2016 Published / External Planned Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included. Spreadsheet Yes PARR Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included. 1 SITE TEXT No No Active The name or identification number of a given site at which samples were collected. TEXT As provided 2 LATITUDE NUMBER No No Active The latitude of the sampling site in decmal degrees. Units for values are decimal degrees. NUMBER decimal degrees As provided 3 LONGITUDE NUMBER No No Active The longitude of the sampling site in decimal degrees. Units for values are decimal degrees. NUMBER decimal degrees As provided 4 DATE DATE No No Active The date (local time) on which as sample was collected. DATE As provided 5 TIME TIME No No Active The time (local time) on the date in which a sample began to be collected. TIME As provided 6 SAMPLEID TEXT No No Active The name or identification number of a individual samples collected at specific sites. IDs incorporate a 2 letter site code followed by the eight digit sampling date and a 2 digit code signifying the sample type. TEXT As provided 7 OA TEXT No No Active The concentration of okadaic acid measured in a sample. Units for values are micrograms per 100 grams of tissue. TEXT micrograms per 100 grams of tissue less than the limit of detection to the measured value 8 DTX-1 TEXT No No Active The concentration of dinophysis toxin 1 measured in a sample. Units for values are micrograms per 100 grams of tissue. TEXT micrograms per 100 grams of tissue less than the limit of detection to the measured value 9 DTX-2 TEXT No No Active The concentration of dinophysis toxin 2 measured in a sample. Units for values are micrograms per 100 grams of tissue. TEXT micrograms per 100 grams of tissue less than the limit of detection to the measured value 10 AZA-1 TEXT No No Active The concentration of azaspiracid 1 measured in a sample. Units for values are micrograms per 100 grams of tissue. TEXT micrograms per 100 grams of tissue less than the limit of detection to the measured value 11 AZA-2 TEXT No No Active The concentration of azaspiracid 2 measured in a sample. Units for values are micrograms per 100 grams of tissue. TEXT micrograms per 100 grams of tissue less than the limit of detection to the measured value 12 AZA-3 TEXT No No Active The concentration of azaspiracid 3 measured in a sample. Units for values are micrograms per 100 grams of tissue. TEXT micrograms per 100 grams of tissue less than the limit of detection to the measured value 13 Total OA equiv. TEXT No No Active The sum of Okadaic acid, Dinophysis toxin 1 and Dinophysis toxin 2 for a given sample after equivalency factors have been applied. Units for values are micrograms per 100 grams of tissue. TEXT micrograms per 100 grams of tissue less than the limit of detection to the measured value 14 PP2A TEXT No No Active Result of the protein phosphatase 2A screening assay. Units for values are micrograms per 100 grams of tissue. TEXT micrograms per 100 grams of tissue less than the limit of detection to the measured value 15 Shellfish Analysis Notes TEXT No No Active Notes regarding the analyses of specific shellfish samples. TEXT As provided Data Steward 2015-10-01 Person Adams, Nicolaus G Nicolaus.Adams@noaa.gov 2725 Montlake Blvd East Seattle WA 98112-2097 206-860-6787 206-860-3335 Distributor 2015-10-01 Organization Northwest Fisheries Science Center NWFSC nmfs.nwfsc.metadata@noaa.gov https://ror.org/05r7z1k40 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov NWFSC Home Online Resource Metadata Contact 2015-10-01 Organization Northwest Fisheries Science Center NWFSC nmfs.nwfsc.metadata@noaa.gov https://ror.org/05r7z1k40 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov NWFSC Home Online Resource Originator 2015-10-01 Person Adams, Nicolaus G Nicolaus.Adams@noaa.gov 2725 Montlake Blvd East Seattle WA 98112-2097 206-860-6787 206-860-3335 Point of Contact 2015-10-01 Person Adams, Nicolaus G Nicolaus.Adams@noaa.gov 2725 Montlake Blvd East Seattle WA 98112-2097 206-860-6787 206-860-3335 -122.3062 -122.3062 47.6449 47.6449 Various locations in WA Continuing 2016-06-06 -122.3062 -122.3062 47.6449 47.6449 NWFSC Montlake Laboratory Continuing 0016-01-01 Unclassified At this time, contact the Data Manager for information on obtaining access to this data set. In the near future, the NWFSC will strive to provide all non-sensitive data resources as a web service in order to meet the NOAA Data Access Policy Directive (https://nosc.noaa.gov/EDMC/PD.DA.php). NA https://www.webapps.nwfsc.noaa.gov/apex/parr/aza_project_seawater_date_for_lipophilic_toxins_in_washington_state_2016/data/page/ 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 AZA Project Seawater Date for Lipophilic Toxins In Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. https://www.webapps.nwfsc.noaa.gov/apex/parr/aza_project_shellfish_data_for_lipophilic_toxins_in_washington_state_2016/data/page/ 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 AZA Project Shellfish Data for Lipophilic Toxins I Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/aza_project_seawater_date_for_lipophilic_toxins_in_washington_state_2016 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 AZA Project Seawater Date for Lipophilic Toxins In Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/aza_project_shellfish_data_for_lipophilic_toxins_in_washington_state_2016 2015-10-01 Organization Northwest Fisheries Science Center https://ror.org/05r7z1k40 AZA Project Shellfish Data for Lipophilic Toxins I Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/104746 Lipophilic Toxins in WA Online Resource Web site NWFSC Dataset Information page. Contains information regarding toxin producing species and toxin measurements in shellfish and seawater samples. Also includes associated environmental data. These data were collected and processed in accordance with established protocols and best practices under the direction of the project’s Principal Investigator. Contact the dataset Data Manager for full QA/QC methodology. No 0 No No No 0 days No Delay NCEI-MD 365 The Northwest Fisheries Science Center facilitates backup and recovery of all data and IT components which are managed by IT Operations through the capture of static (point-in-time) backup data to physical media. Once data is captured to physical media (every 1-3 days), a duplicate is made and routinely (weekly) transported to an offsite archive facility where it is maintained throughout the data's applicable life-cycle. NA 54712 Entity 54713 Entity gov.noaa.nmfs.inport:30880 Jeffrey Cowen 2016-02-24T10:06:38 SysAdmin InPortAdmin 2026-03-04T19:17:17 2019-06-04 Northwest Fisheries Science Center NWFSC 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov 1001 Public No No 2019-06-04 1 Year 2020-06-04