32188 AFSC/NMML/CCEP: Sampling locations for harbor seal genetics in Washington and British Columbia Data Set Published / External 23110 California Current Ecosystems Program Project Completed Using skin samples from 777 unweaned pups collected in 9 different regions in WA state and British Columbia (WA Coastal Estuaries, WA North Coast, British Columbia, Boundary Bay, San Juan Islands, Smith/Minor Islands, Dungeness Spit, Hood Canal, South Puget Sound), the California Current Ecosystem Program at the Marine Mammal Laboratory/AFSC tested levels of genetic variation using mtDNA and 9 microsatellite loci. For both mtDNA and microsatellites we found the same four groupings. Previously, harbor seals were managed as two stocks: coastal and Inland waters. The data indicated that the harbor seals should be managed as four stocks: one on the Coast and three stocks in the Inland Waters. An additional 92 skin samples were collected opportunistically from non-pups. They have not yet been analyzed. The CCEP of MML collected genetic samples from pups to determine stocks of harbor seals on Washington Coast and Inland Waters. Genetic samples from other age classes were also collected. Cite data as: Huber, H.R., B.R. Dickerson, D.M. Lambourn, S.J.Jeffries (2016). Sampling location for harbor seal genetics in Washington and British Columbia from 1993-08-25 to 2009-09-23 (NCEI Accession 0148458). NOAA National Centers for Environmental Information. Theme Genetics Theme Microsatellite DNA Theme Mitochondrial DNA Theme Pacific harbor seal Theme Phoca vitulina Theme Stock Definition Temporal Pupping season Spatial British Columbia, Canada Spatial Washington Inland Waters Spatial Washington coast Alaska Fisheries Science Center Seattle WA USA Data Set CSV Files As Needed Table (digital) The user is responsible for the results of any application of this data for other than its intended purpose. NOAA denies liability if the data are misused. Applications or inferences derived from the data should be carefully considered for accuracy. Whereas every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by any errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty. N/A N/A N/A 32189 Collection Location and Date Published / External Completed 1 Date UNKNOWN Yes No Active Sample collection date 2 Region UNKNOWN Yes No Active Geographical region where sample was collected 3 Area UNKNOWN Yes No Active Specific geographic area within Region where sample was collected. 4 Sample# UNKNOWN Yes No Active Number assigned to MERL (AFSC) sample. 5 Age UNKNOWN No No Active Age class of individual at sampling. 6 Condition UNKNOWN No No Active Condition of animal (e.g. ALIVE, DEAD) at time of sampling. 7 Sex UNKNOWN No No Active Sex of individual. 8 TagColor UNKNOWN No No Active Color of flipper tag attached to sampled individual including Red, Blue, and Yellow. 9 Tag# UNKNOWN No No Active Number on flipper tag for sampled individual. 10 Comments UNKNOWN No No Active Additional information about sampled individual or other pertinent comments. 11 AdditionalID UNKNOWN No No Active Identifications given by rehabilitation centers or stranding networks. 12 Z_Lab#_SWFSC UNKNOWN No No Active Genetic samples processed at the Southwest Fisheries Science Center (SWFSC) were split and part of the sample remained at the SWFSC with the Z_Lab#. Data Steward 2016 Person Huber, Harriet harriet.huber@noaa.gov Distributor 2016 Organization NOAA National Centers for Environmental Information NCEI ncei.info@noaa.gov https://ror.org/04r0wrp59 https://www.ncei.noaa.gov/contact NCEI Contact Information Information for contacts at NCEI. Online Resource Metadata Contact 2016 Person Orr, Tony tony.orr@noaa.gov Originator 2016 Person Huber, Harriet harriet.huber@noaa.gov Point of Contact 2016 Person Huber, Harriet harriet.huber@noaa.gov Point of Contact 2016 Person Delong, Robert robert.delong@noaa.gov -127 -122 50 46 Western Washington State Coast, British Columbia, and Inland waters (i.e. Salish Sea) Unclassified None. None. The dataset is archived with the NOAA National Centers for Environmental Information (NCEI). Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these data is suggested. Communication and collaboration with dataset authors is strongly encouraged prior to use. There are no legal restrictions on access to the data. They reside in public domain and can be freely distributed. User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. Data must be cited as: Huber, H.R., B.R. Dickerson, D.M. Lambourn, S.J.Jeffries (2016). Sampling location for harbor seal genetics in Washington and British Columbia from 1993-08-25 to 2009-09-23 (NCEI Accession 0148458). NOAA National Centers for Environmental Information. None. Metadata are subject to revision at any time and information within the metadata record should not be cited without author consent. Whereas every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by any errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty. https://data.nodc.noaa.gov/cgi-bin/iso?id=gov.noaa.nodc:0148458 2016 Organization NOAA National Centers for Environmental Information https://ror.org/04r0wrp59 CCEP Harbor Seal Genetic Location Information.csv csv (comma-separated values) CSV - Comma Separated Values (Text) http://www.nrcresearchpress.com/doi/pdf/10.1139/Z09-141 Online Resource PDF Huber, H.R., B.R. Dickerson, S.J. Jeffries, and D.M. Lambourn. 2012. Genetic analysis of Washington State harbor seals (Phoca vitulina richardii) using microsatellites. Canadian Journal of Zoology 90:1361-1369. http://www.nrcresearchpress.com/doi/pdf/10.1139/Z09-141 Online Resource PDF Huber, H.R., S.J. Jeffries, D.M. Lambourn, and B.R. Dickerson. 2010. Population substructure of harbor seals (Phoca vitulina richardsi) in Washington State using mtDNA. Canadian Journal of Zoology 88:280-288. MS Excel Data were proofed for consistency and logic in the field and in the laboratory. No 0 No No Yes Unknown Data are not automatically processed NCEI-MD Unknown IT Security and Contingency Plan for the system establishes procedures and applies to the functions, operations, and resources necessary to recover and restore data as hosted in the Western Regional Support Center in Seattle, Washington, following a disruption. Capture methods were by beach seine or tangle net or 'run and grab;' methods are described in Jeffries et al. 1993. Genetic samples were acquired using a 6mm skin punch. Samples not collected by MML were collected by Wolf Hollow Wildlife Rehabilitation Center (San Juan Island, WA), Sarvey Wildlife Center (Arlington, WA), Washington Fish and Wildlife Department (Tacoma, WA) necropsies, and Cascadia Research Collective (Olympia, WA) necropsies. Samples were analyzed at Southwest Fisheries Science Center (La Jolla, CA), Northwest Fisheries Science Center (Seattle, WA), and at MERL/AFSC (Seattle, WA). 32189 Entity gov.noaa.nmfs.inport:32188 Tony Orr 2016-04-08T14:36:43 SysAdmin InPortAdmin 2026-03-04T19:17:17 2019-04-15 Alaska Fisheries Science Center AFSC 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA https://www.afsc.noaa.gov 0700-1700 Pacific Time 1001 Public No No 2019-04-15 1 Year 2020-04-15