35877 AFSC/RACE/SAP/Long: Data from: Upper thermal tolerance in red and blue king crab: Sublethal and lethal effects Data Set Published / External 22354 Shellfish Assessment Program Project Completed This dataset contains data from a series of experiments that determined the upper thermal tolerance of early benthic stage red and blue king crabs. Experiments included determining the temperature at which 50% of crabs died after 24 hour exposure, determining the effect of temperature on feeding ration, and the effects of temperature on long-term growth and mortality. This data documents important biological references points for red and blue king crab in regards to their temperature tolerance that will help in predicting how they will responds if temperatures rise within their range. Loaded by FGDC Metadata Uploader, batch 9550, 12-06-2016 14:01 The following FGDC sections are not currently supported in InPort, but were preserved and will be included in the FGDC export: - Taxonomy (FGDC:taxonomy) Theme ISO 19115 Topic Category oceans Theme growth Theme habitat Theme juvenile Theme mortality Theme survival Theme water temperature Spatial Alaska Spatial Bering Sea Spatial Bristol Bay Spatial Pribilof Islands Kodiak Fisheries Research Center Kodiak AK USA Data Set As Needed Table (digital) N/A N/A N/A 35878 Feeding Published / External Completed Data from feeding experiments. Loaded by FGDC Metadata Uploader, batch 9550, 12-06-2016 14:01 Data from feeding experiments. ID Unknown No No Active A sequence of unique integers that identifies each observation A sequence of unique integers that identifies each observation Experiment/Treatment VARCHAR2 No No Active A catigorical atribute that identifies which expeirment and which treatment each observation was made from. Multiple Replicate VARCHAR2 No No Active Identifies the replicate number. Each crab within each treatment is given a uniqe number. Each control trial (where run) is given a unique number. multiple Food mass start NUMBER No No Active Food mass in grams at the beginning of the experiment grams 0 - 1 Food mass end NUMBER No No Active Food mass in grams at the end of the experiment grams 0 - 1 Crab mass NUMBER No No Active The wet mass of the crab in grams for each trial. Control trials have no crabs present. grams 0 - 1 Food ration NUMBER No No Active This coloumn is calculated as the amount of food eaten by each crab corrected for the change of mass of food held in the control trial expressed as a proportion of the crab's wet mass. A minimum value of 0 is assumed. Controls are blank as they contained no crabs. mass of food eaten/mass of crab 0 - 100 35879 Growth Published / External Completed Contains data on the starting and ending sizes of crabs in the growth experiment. Loaded by FGDC Metadata Uploader, batch 9550, 12-06-2016 14:01 Contains data on the starting and ending sizes of crabs in the growth experiment. ID Unknown No No Active A sequence of unique integers that identifies each observation A sequence of unique integers that identifies each observation Experiment/Treatment VARCHAR2 No No Active A catigorical atribute that identifies which expeirment and which treatment each observation was made from. Multiple Species VARCHAR2 No No Active Identifies which species observation was made on. RKC, BKC Crab number VARCHAR2 No No Active A number given to each crab within each expeirment and treatment to identify it. 1-15 CW Start NUMBER No No Active The carapace width (including spines) at the beginning of the experiment. millimeters 0 - 15 CW End NUMBER No No Active The carapace width (including spines) at the end of the experiment. Blanks indicate crab died and was not measured. millimeters 0 - 15 35880 LT50 Published / External Completed Data on the number of crabs alive and moving at the end of the acute temperature tolerance experiment Loaded by FGDC Metadata Uploader, batch 9550, 12-06-2016 14:01 Data on the number of crabs alive and moving at the end of the acute temperature tolerance experiment ID Unknown No No Active A sequence of unique integers that identifies each observation A sequence of unique integers that identifies each observation Species VARCHAR2 No No Active Identifies which species observation was made on. RKC, BKC Experiment/Treatment VARCHAR2 No No Active A catigorical atribute that identifies which expeirment and which treatment each observation was made from. Multiple Initial number NUMBER No No Active Number of crabs exposed to the treatment conditions crabs 1 - 14 Dead NUMBER No No Active The number of crabs that were dead after 24 hours exposure to treatment temperature crabs 1 - 14 Move NUMBER No No Active The number of crabs that were able to move after 24 hours exposure to treatment temperature crabs 1 - 14 35881 Molting mortality days Published / External Completed Table indicating the days crab in the long-term growth and survival experiment molted or died. Loaded by FGDC Metadata Uploader, batch 9550, 12-06-2016 14:01 Table indicating the days crab in the long-term growth and survival experiment molted or died. ID Unknown No No Active A sequence of unique integers that identifies each observation A sequence of unique integers that identifies each observation Experiment/Treatment VARCHAR2 No No Active A catigorical atribute that identifies which expeirment and which treatment each observation was made from. Multiple Species VARCHAR2 No No Active Identifies which species observation was made on. RKC, BKC Crab number VARCHAR2 No No Active A number given to each crab within each expeirment and treatment to identify it. 1-15 Molt day NUMBER No No Active Day a molting event occred. Days are given as the number of days since the experiment was begun. Experiment start date was November 11, 2012. Blanks indicate a crab died before molting. Some crabs did not molt or die during the experiment. days 1 - 45 Mortality day NUMBER No No Active Day a crab died. Days are given as the number of days since the experiment was begun. Experiment start date was November 11, 2012. Blanks indicate the crab did not die during the experiment. Some crabs did not molt or die during the experiment. days 1 - 45 35882 Temperatures Published / External Completed Temperatures recoded during all experiments Loaded by FGDC Metadata Uploader, batch 9550, 12-06-2016 14:01 Temperatures recoded during all experiments ID Unknown No No Active A sequence of unique integers that identifies each observation A sequence of unique integers that identifies each observation Experiment/Treatment VARCHAR2 No No Active A catigorical atribute that identifies which expeirment and which treatment each observation was made from. Multiple Date,Time Unknown No No Active Date and time of an observation. TImes are local times in Kodiak, AK. Date and time in: mm/dd/yyyy hh:mm Temperature NUMBER No No Active Temperature in degreees Celsius. degrees Celsius 0 - 30 Data Steward 2016 Person Long, Chris chris.long@noaa.gov Distributor 2016-12-06 Person Long, Chris chris.long@noaa.gov Originator 2016-12-06 Organization West Coast Regional Office WCRO 501 West Ocean Blvd Long Beach CA 90802-4213 https://www.westcoast.fisheries.noaa.gov/ Originator 2016-12-06 Organization Alaska Fisheries Science Center AFSC afsc.webmaster@noaa.gov https://ror.org/01h7fye62 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA (206) 526-4000 (206) 526-4004 https://www.fisheries.noaa.gov/about/alaska-fisheries-science-center AKFSC Home Page Online Resource 0700-1700 Pacific Time Point of Contact 2016-12-06 Person Long, Chris chris.long@noaa.gov Ground Condition -152.395268 -152.395268 57.782403 57.782403 Laboratory study in Kodiak Alaska Range 2012 2013 Unclassified unknown There are no legal restrictions on access to the data. They reside in public domain and can be freely distributed. User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. https://console.cloud.google.com/storage/browser/nmfs_odp_afsc/RACE/SAP/Long%3B%20Data%20from%3B%20Upper%20thermal%20tolerance%20in%20red%20and%20blue%20king%20crab%3B%20Sublethal%20and%20lethal%20effects Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/16/2021. Contact: Daniel.woodrich@noaa.gov Data was checked for outliers and for values out of expected range. Not applicable. Not applicable. Data was checked for outliers and for values out of expected range. No Unknown No No Yes unknown no delay NCEI-MD unknown IT Security and Contingency Plan for the system establishes procedures and applies to the functions, operations, and resources necessary to recover and restore data as hosted in the Western Regional Support Center in Seattle, Washington, following a disruption. 1 Initially, seven different temperatures were tested for both species: 14, 16, 18, 20, 22, 24, 26°C. As the trials were performed, we added an additional temperature treatment for each species at around the estimated LT50 ( temperature that causes 50% mortality): 21°C for blue king crab and 25°C for red king crab. The trials were run in a random order. For the seven temperatures tested in common among the species, both species were tested simultaneously. For each trial, 14 year-0 red and blue king crab were haphazardly assigned to each treatment. At the beginning of the experiments, all the tanks were at ambient temperature (i.e., the same temperature the crabs were being held at). The temperature in each tank was then increased gradually to the experimental temperature over 4 hours. The crabs were left at the temperature for 24 h after which they were checked for activity by gently prodding them with a dissecting needle and observing whether they responded by moving away from the needle. They were then checked to see if they were alive. 2 In this experiment, the effects of temperature on feeding ration were determined for red and blue king crabs. Temperatures tested included 11, 13, 15, 17, 20, and 23°C for red king crab and 4, 6, 9, 11, 13, 15, 17, and 20°C for blue king crab.Year-1 blue king crab and year-0 red king crab were used. In each trial 5 (blue king crab) or 7 (red king crab) crab were massed and then introduced into individual holding cells. The temperature was slowly adjusted to the treatment temperature over 4 h. Crab were allowed to rest for 24h to acclimate them to the temperature and to standardize hunger levels. Then crab were fed small pieces of massed squid mantle and allowed to feed for 24h. At the same time, pieces of food were also placed in three control cells without crab. After this, the remaining food was removed and massed. Daily feeding ration, expressed as a percentage of the crab’s mass, was calculated for each crab and corrected for the change of mass of the food in the control cells assuming a minimum feeding ration of 0%. In a few cases, crab molted during the trial or ate all the food and the data from these crab was excluded from analysis. 3 To determine how temperature affects growth and long-term survival in year-0 crabs we performed 45 day experiments. Red king crab were tested at 12, 15, and 20°C and blue king crab at 12, 15, and 17°C. Twelve crabs per species per treatment were haphazardly selected. Crabs had their carapace width measured and were introduced into individual holding cells. The temperature was slowly adjusted to the treatment temperature over 4 h. Crabs were checked daily for molting or mortality and were fed to excess. After the end of the experiment each crab had their carapace width measured a second time. 35878 Entity 35879 Entity 35880 Entity 35881 Entity 35882 Entity gov.noaa.nmfs.inport:35877 Claire Armistead 2016-12-06T14:01:40 SysAdmin InPortAdmin 2026-03-04T19:17:17 2016-12-06 Alaska Fisheries Science Center AFSC 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA https://www.afsc.noaa.gov 0700-1700 Pacific Time 1001 Public No No 2016-12-06 1 Year 2017-12-06 none invertebrates crab Kingdom Animalia Genus Paralithodes Species camtschaticus red king crab Genus Paralithodes Species platypus blue king crab Feeding Data from feeding experiments. NA ID A sequence of unique integers that identifies each observation n/a A sequence of unique integers that identifies each observation Experiment/Treatment A catigorical atribute that identifies which expeirment and which treatment each observation was made from. n/a Multiple Experiments include Feeding for the feeding ration expeirment, Growth for the long-term growth expeirment, LT50 for the acute thermal tolerance experiment. RKC indicates red king crab only trials, BKC indicates blue king crab trials. Numbers followed by "C" indicate the nominal temperature treatment. n/a Replicate Identifies the replicate number. Each crab within each treatment is given a uniqe number. Each control trial (where run) is given a unique number. n/a multiple "Crab" followed by a number or just a number indicates a replicate crab. "Control" followed by a number indicates a replicate control trial with no crab present. n/a Food mass start Food mass in grams at the beginning of the experiment n/a/ 0 1 grams 0.0001 Food mass end Food mass in grams at the end of the experiment n/a 0 1 grams 0.0001 Crab mass The wet mass of the crab in grams for each trial. Control trials have no crabs present. n/a 0 1 grams 0.0001 Food ration This coloumn is calculated as the amount of food eaten by each crab corrected for the change of mass of food held in the control trial expressed as a proportion of the crab's wet mass. A minimum value of 0 is assumed. Controls are blank as they contained no crabs. n/a 0 100 mass of food eaten/mass of crab Growth Contains data on the starting and ending sizes of crabs in the growth experiment. n/a ID A sequence of unique integers that identifies each observation n/a A sequence of unique integers that identifies each observation Experiment/Treatment A catigorical atribute that identifies which expeirment and which treatment each observation was made from. n/a Multiple Experiments include Feeding for the feeding ration expeirment, Growth for the long-term growth expeirment, LT50 for the acute thermal tolerance experiment. RKC indicates red king crab only trials, BKC indicates blue king crab trials. Numbers followed by "C" indicate the nominal temperature treatment. n/a Species Identifies which species observation was made on. n/a RKC, BKC RKC- red king crab BKC- blue king crab n/a Crab number A number given to each crab within each expeirment and treatment to identify it. n/a 1-15 Number indicating each crab within each experiment and treatment. n/a CW Start The carapace width (including spines) at the beginning of the experiment. n/a 0 15 millimeters 0.01 CW End The carapace width (including spines) at the end of the experiment. Blanks indicate crab died and was not measured. n/a 0 15 millimeters 0.01 LT50 Data on the number of crabs alive and moving at the end of the acute temperature tolerance experiment n/a ID A sequence of unique integers that identifies each observation n/a A sequence of unique integers that identifies each observation Species Identifies which species observation was made on. n/a RKC, BKC RKC- red king crab BKC- blue king crab n/a Experiment/Treatment A catigorical atribute that identifies which expeirment and which treatment each observation was made from. n/a Multiple Experiments include Feeding for the feeding ration expeirment, Growth for the long-term growth expeirment, LT50 for the acute thermal tolerance experiment. RKC indicates red king crab only trials, BKC indicates blue king crab trials. Numbers followed by "C" indicate the nominal temperature treatment. n/a Initial number Number of crabs exposed to the treatment conditions n/a 1 14 crabs Dead The number of crabs that were dead after 24 hours exposure to treatment temperature n/a 1 14 crabs Move The number of crabs that were able to move after 24 hours exposure to treatment temperature n/a 1 14 crabs Molting mortality days Table indicating the days crab in the long-term growth and survival experiment molted or died. n/a ID A sequence of unique integers that identifies each observation n/a A sequence of unique integers that identifies each observation Experiment/Treatment A catigorical atribute that identifies which expeirment and which treatment each observation was made from. n/a Multiple Experiments include Feeding for the feeding ration expeirment, Growth for the long-term growth expeirment, LT50 for the acute thermal tolerance experiment. RKC indicates red king crab only trials, BKC indicates blue king crab trials. Numbers followed by "C" indicate the nominal temperature treatment. n/a Species Identifies which species observation was made on. n/a RKC, BKC RKC- red king crab BKC- blue king crab n/a Crab number A number given to each crab within each expeirment and treatment to identify it. n/a 1-15 Number indicating each crab within each experiment and treatment. n/a Molt day Day a molting event occred. Days are given as the number of days since the experiment was begun. Experiment start date was November 11, 2012. Blanks indicate a crab died before molting. Some crabs did not molt or die during the experiment. n/a 1 45 days Mortality day Day a crab died. Days are given as the number of days since the experiment was begun. Experiment start date was November 11, 2012. Blanks indicate the crab did not die during the experiment. Some crabs did not molt or die during the experiment. n/a 1 45 days Temperatures Temperatures recoded during all experiments n/a ID A sequence of unique integers that identifies each observation n/a A sequence of unique integers that identifies each observation Experiment/Treatment A catigorical atribute that identifies which expeirment and which treatment each observation was made from. n/a Multiple Experiments include Feeding for the feeding ration expeirment, Growth for the long-term growth expeirment, LT50 for the acute thermal tolerance experiment. RKC indicates red king crab only trials, BKC indicates blue king crab trials. Numbers followed by "C" indicate the nominal temperature treatment. n/a Date,Time Date and time of an observation. TImes are local times in Kodiak, AK. n/a Date and time in: mm/dd/yyyy hh:mm Temperature Temperature in degreees Celsius. n/a 0 30 degrees Celsius