36975 Vibrio Parahaemolyticus Survey Washington 2007-2008 Entity Published / External 17958 Vibrio ecology in PNW - The Ecology of Vibrio parahaemolyticus in the Pacific Northwest: Implications for risk assessment and early warning systems Data Set Planned Results of a survey for the bacterium Vibrio parahaemolyticus in oyster growing areas of Washington State, 2007-2008. Spreadsheet Yes PARR Results of a survey for the bacterium Vibrio parahaemolyticus in oyster growing areas of Washington State, 2007-2008. 1 Sample # NUMBER No No Active Unique identifier for the sample. NUMBER 2 Date collected DATE No No Active The date on which physical data and/or biological sample was collected. DATE 3 Sampling time DATE No No Active Time of sampling given as military time. Units for values are Hour:Minute. DATE Hour:Minute 4 Time of low tide DATE No No Active The most recent low tide closest to the time of the sampling given as military time. Units for values are Hour:Minute. DATE Hour:Minute 5 Julian day NUMBER No No Active The day number for a year. January 1 is Julian day 1 while February 1 is Julian day 32, etc. NUMBER 6 Site code VARCHAR2 No No Active A code assigned to the the sampling site by the Washington State Department of Health. VARCHAR2 7 Site name VARCHAR2 No No Active The name of the sampling site. VARCHAR2 8 Site latitude NUMBER No No Active The site latitude, which along with the site longitude unambiguously specifies the site location. NUMBER 9 Site longitude NUMBER No No Active The site longitude,which along with the site latitude unambiguously specifies the site location. NUMBER 10 Air temperature NUMBER No No Active Air temperature at the sampling site. Units for values are Degrees Centigrade (C). NUMBER Degrees Centigrade (C) 11 Surface water temperature NUMBER No No Active The temperature of the surface water at the sampling site. Units for values are Degrees Centigrade (C). NUMBER Degrees Centigrade (C) 12 Water temperature at -3 feet NUMBER No No Active The temperature of water 3 feet below the surface at the sampling site. Units for values are Degrees Centigrade (C). NUMBER Degrees Centigrade (C) 13 Salinity NUMBER No No Active Salinity of the water at the sampling site. Units for values are Parts per thousand (ppt). NUMBER Parts per thousand (ppt) 14 Chlorophyll NUMBER No No Active The chlorophyll content of the water at the sampling site. Units for values are Micrograms per liter of water. NUMBER Micrograms per liter of water 15 Oyster tissue temperature NUMBER No No Active The temperature of oyster tissue collected at the sampling site. Units for values are Degrees Centigrade (C). NUMBER Degrees Centigrade (C) 16 Total Vibrio parahaemolyticus, water NUMBER No No Active Each cell of Vibrio parahaemolyticus (Vp) contains one copy of a gene called tl. Each water sample was analyzed for the number of copies of tl giving a direct measure of the density of Vp in the water column. Not all Vp are virulent. Units for values are Number of cells of Vp per 100 grams of water. NUMBER Number of cells of Vp per 100 grams of water 17 tdh+ Vibrio parahaemolyticus, water NUMBER No No Active The tdh gene is associated with virulence. Each cell of Vp can contain more than one copy of tdh. Therefore, the number of copies of tdh can exceed the number of copies of tl in some samples. Units for values are Number of cells of potentially pathogenic Vp per 100 grams of water. NUMBER Number of cells of potentially pathogenic Vp 100 gm of water 18 Total Vibrio parahaemolyticus, net tow NUMBER No No Active At most sites, a large volume of water was filtered through a plankton net with a mesh size of 200 micron. This concentrates particate matter such as plankton to which Vp bacteria can attach. As before, the number of copeis of the tl gene serves to give a direct measure of the density of Vp associated with particles in the water column. Units for values are Number of cells of Vp per 100 grams of water retained in the net tow. NUMBER Number of cells of Vp per 100 gm of water retained net tow. 19 tdh+ Vibrio parahaemolyticus, net tow NUMBER No No Active The number of copies of the virulence-assciated tdh gene detected in net tow samples. Units for values are Number of copies of tdh present per 100 grams of water retained in the net tow. NUMBER Number of copies of tdh per 100 gm of water retained net tow 20 Total Vibrio parahemolyticus, oyster NUMBER No No Active Oyster tissue is homogenized and analyzed for total V. parahaemolyticus by detection of the the tl gene by the "Most Probable Number (MPN)" method. Units for values are The MPN of Vp per gram of oyster tissue. NUMBER The MPN of Vp per gram of oyster tissue. 21 tdh+ Vibrio parahaemolyticus, oyster NUMBER No No Active The most probable number of copies of the virulence-associated tdh gene detected in oyster tissue. Units for values are The MPN of copies of tdh per gram of oyster tissue. NUMBER The MPN of copies of tdh per gram of oyster tissue. gov.noaa.nmfs.inport:36975 Jeffrey Cowen 2017-02-28T13:01:47 SysAdmin InPortAdmin 2022-08-09T17:11:18 2018-02-27 Northwest Fisheries Science Center NWFSC 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov 1001 Public No 2018-02-27 1 Year 2019-02-27