39258
National Status and Trends, Benthic Surveillance Project DNA-Xenobiotic Adducts Data, 1991, National Centers for Coastal Ocean Science
bsdna_h
Data Set
Published / External
37215
NSandT
Project
Completed
1992
In order to determine the current status of and to detect any long-term trends in the environmental quality of U.S. nearshore waters, NOAA initiated the National Status and Trends program in 1984 with its National Benthic Surveillance Project. The primary objective of the Benthic Surveillance Project was to quantify concentrations of a suite of organic and inorganic contaminants in the livers of fish and surficial sediments from selected sites in the coastal and estuarine waters of the United States. In addition, the levels of certain indicators of the biological effects of these contaminants were measured. Incidences of visible lesions, including fin erosion, have been noted and histopathological examinations of various tissues have been carried out. Originally histopathological examinations determined the prevalence of any identifiable disease conditions in samples of liver, kidney, and gill tissue.In addition, the Benthic Surveillance Project tested fish livers from 30 locations in 1991 to identify the concentrations of DNA xenobiotic adducts. DNA xenobiotic adducts are a bioindicator of exposure to genotoxic compounds. Measurement of biological markers such as DNA xenobiotic adducts have been used to improve the evaluation of contaminant exposure and responses in indigenous fish, and thus reinforce the connection between exposure to toxic chemicals and the observed injuries.
In response to concerns over environmental quality of the Nation's coastal and estuarine ecosystems, NOAA created the National Status and Trends (NSandT) Program in 1984. From 1984 through 1993, the Benthic Surveillance Project monitored chemical concentrations in the livers (and for metabolites of PAH's in the bile) of bottom-dwelling fish and in sediments at the sites of fish capture. The Benthic Surveillance Project also measured the biological effects of contaminant exposure, primarily as prevalence's of toxicopathic liver diseases.
982
Theme
ISO 19115 Topic Category
environment
Theme
Coastal Monitoring
Theme
DNA
Theme
DNA abnormalities
Theme
DNA adducts
Theme
DNA xenobiotic adducts
Theme
NCCOS Research Data Type > Field Observation
Theme
NCCOS Research Priority > Long-term Monitoring
Theme
NCCOS Research Priority > Stressors, Impacts, Mitigation, and Restoration (SIMR)
Theme
NCCOS Research Topic > Bioeffects/Toxicity
Theme
NCCOS Research Topic > Chemical Contaminants
Theme
NCCOS Research Topic > Monitoring
Theme
NSandT
Theme
deoxyribonucleic acid
Theme
genotoxic exposure
Theme
oceans
Theme
xenobiotic deoxyribonucleic acid adducts
Spatial
Baltimore Harbor, Brewerton Channel
Spatial
Boston Harbor, Deer Island
Spatial
Cape Elizabeth, Richmond Island
Spatial
Cape Fear River, Horseshoe Shaol
Spatial
Charlotte Harbor, Cape Haze
Spatial
Chesapeake Bay, Chester River
Spatial
Chesapeake Bay, James River
Spatial
Chesapeake Bay, Patuxent River
Spatial
Choctawhatchee Bay, Choctawhatchee Bay
Spatial
Dana Point Harbor, Outside
Spatial
Delaware Bay, Cherry Island Range
Spatial
Johns Bay, Pemaquid Neck
Spatial
Lower Laguna Madre, Laguna Heights
Spatial
Lower Laguna Madre, Long Island
Spatial
NCCOS Research Location > Geographic Area > Coastal Ocean
Spatial
NCCOS Research Location > Region > Alaska
Spatial
NCCOS Research Location > Region > East Coast
Spatial
NCCOS Research Location > Region > Gulf of Mexico
Spatial
NCCOS Research Location > Region > West Coast
Spatial
NCCOS Research Location > U.S. States and Territories > Alabama
Spatial
NCCOS Research Location > U.S. States and Territories > Alaska
Spatial
NCCOS Research Location > U.S. States and Territories > California
Spatial
NCCOS Research Location > U.S. States and Territories > Connecticut
Spatial
NCCOS Research Location > U.S. States and Territories > Delaware
Spatial
NCCOS Research Location > U.S. States and Territories > Florida
Spatial
NCCOS Research Location > U.S. States and Territories > Georgia
Spatial
NCCOS Research Location > U.S. States and Territories > Louisiana
Spatial
NCCOS Research Location > U.S. States and Territories > Maine
Spatial
NCCOS Research Location > U.S. States and Territories > Maryland
Spatial
NCCOS Research Location > U.S. States and Territories > Massachusetts
Spatial
NCCOS Research Location > U.S. States and Territories > Mississippi
Spatial
NCCOS Research Location > U.S. States and Territories > New Jersey
Spatial
NCCOS Research Location > U.S. States and Territories > New York
Spatial
NCCOS Research Location > U.S. States and Territories > North Carolina
Spatial
NCCOS Research Location > U.S. States and Territories > Oregon
Spatial
NCCOS Research Location > U.S. States and Territories > Rhode Island
Spatial
NCCOS Research Location > U.S. States and Territories > South Carolina
Spatial
NCCOS Research Location > U.S. States and Territories > Texas
Spatial
NCCOS Research Location > U.S. States and Territories > Virginia
Spatial
NCCOS Research Location > U.S. States and Territories > Washington
Spatial
New Bedford Harbor, Clarks Point
Spatial
Pascagoula River, Escatawpa River
Spatial
Pensacola Bay, Pensacola Bay
Spatial
Raritan Bay, East Reach
Spatial
Raritan Bay, Upper Bay
Spatial
Salem Harbor, Folger Point
Spatial
San Antonio Bay, Mosquito Point
Spatial
San Diego Bay, North
Spatial
San Diego Bay, Twenty Eighth Street
Spatial
San Francisco Bay, Hunters Point
Spatial
San Francisco Bay, Southampton Shoal
Spatial
San Pedro Bay, Outer Harbor
Spatial
Sapelo Sound, Dog Hammock
Spatial
St. Andrews Bay, Military Point
Spatial
St. Johns River, Arlington Channel
Spatial
St. Lucie River, Stuart
Spatial
U.S. Exclusive Economic Zone
National Centers for Coastal Ocean Science
Silver Spring
MD
Data Set
None Planned
Table (digital)
None
41238
Dataset Parameters for Benthic Surveillance DNA Xenobiotic Adducts data
Dataset Parameters for Benthic Surveillance DNA Xenobiotic Adducts data
Published / External
Completed
The BSdna_h data file reports information regarding DNA xenobiotic adducts study parameters. | Description Source: National Status and Trends, Benthic Surveillance Program
Converted from FGDC using 'fgdc_to_inport_xml_entity.pl' script.
1
NST_SITE
VARCHAR
No
No
Active
5 letter code site identifier. | Description Source: NSandT, Benthic Surveillance Project
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts: National Status and Trends, Benthic Surveillance Project
2
YEAR
VARCHAR
No
No
Active
Year the site was sampled. | Description Source: NSandT, Benthic Surveillance Project
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts: National Status and Trends, Benthic Surveillance Project
3
SPEC_CD1
VARCHAR
No
No
Active
Species abbreviation, usually a 2 or 3 letter code which identifies the common species name. For example AF is used to define Artic Flounder. | Description Source: NSandT, Benthic Surveillance Project
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts: National Status and Trends, Benthic Surveillance Project
4
REP
VARCHAR
No
No
Active
Replicate sample, replicate of a unique sample. | Description Source: NSandT, Benthic Surveillance Project
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts: National Status and Trends, Benthic Surveillance Project
5
SPEC_NO
VARCHAR
No
No
Active
Laboratory specimen number. | Description Source: NSandT, Benthic Surveillance Project
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts: National Status and Trends, Benthic Surveillance Project
6
OTHER_CD
VARCHAR
No
No
Active
Another site identifier, Codes used by NSandT participating laboratories. | Description Source: NSandT, Benthic Surveillance Project
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts: National Status and Trends, Benthic Surveillance Project
7
PPL
VARCHAR
No
No
Active
DNA Xenobiotic adducts. Phosphorus 32 post-labeling. | Description Source: NSandT, Benthic Surveillance Project
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts: National Status and Trends, Benthic Surveillance Project
8
REV_DNA
VARCHAR
No
No
Active
Last revision date of the DNA adducts file. | Description Source: NSandT, Benthic Surveillance Project
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts: National Status and Trends, Benthic Surveillance Project
Data Steward
1992
Position
NCCOS Scientific Data Coordinator
NCCOS.data@noaa.gov
Distributor
1992
Position
NCCOS Scientific Data Coordinator
NCCOS.data@noaa.gov
Metadata Contact
1992
Position
NCCOS Scientific Data Coordinator
NCCOS.data@noaa.gov
Point of Contact
1992
Position
NCCOS Scientific Data Coordinator
NCCOS.data@noaa.gov
Principal Investigator
1992
Person
Piniak, Greg
greg.piniak@noaa.gov
Publication Date
-166.5
-67.3
70.5
25.6
Discrete
1991
Yes
Unclassified
None
NOAA requests that all individuals who download NSandT data acknowledge the source of these data in any reports, papers, or presentations. If you publish these data, please include a statement similar to: "Some or all of the data described in this article were produced by NOAA through its National Status and Trends Program".
https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Live Data and Maps
ASCII
Text (Unstructured)
https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Live Data and Maps
XML
XML (Structured Text)
https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Online Resource
2016-11-16
Date that the source FGDC record was last modified.
2017-04-05
Converted from FGDC Content Standards for Digital Geospatial Metadata (version FGDC-STD-001-1998) using 'fgdc_to_inport_xml.pl' script. Contact Tyler Christensen (NOS) for details.
2017-09-13
Partial upload of Spatial Info section only.
2018-02-08
Partial upload of Positional Accuracy fields only.
Microsoft Windows 2000 Version 5.0 (Build 2195) Service Pack 3; ESRI ArcCatalog 8.3.0.800
The same kind of field/site data have been supplied since the start of the Benthic Surveillance Project, in 1984. Original site coordinates were derived from Loran-C time conversions. Early sites information resulting from Loran-C was converted from time delay information to latitudes and longitudes. These earlier data may be suspect when sites were located close to large structures that could have interfered with accurate time delays. When GPS was first available the signal was intentionally degrades so earlier coordinate information, even if it resulted from GPS, is not as accurate as data would be today. Because fish are not sessile, fish trawls have been made along different tracks in the water body of interest. The latitude/longitude coordinates provided in this file represent a nominal site center and trawling occurs within a 1 km radius of this location.
The quality of the analytical data generated by the NSandT Program is overseen by the QA Project component, which has been in operation since 1985 and is designed to document sampling and analytical procedures, and to reduce intralaboratory and interlaboratory variation. The QA Project documentation will facilitate comparisons between different monitoring programs with similar QA activities and thus will extend the temporal and spatial scale of such programs. To document laboratory expertise, the QA Project requires all NSandT laboratories to participate in a continuing series of intercomparison exercises utilizing a variety of materials. The organic analytical intercomparison exercises are coordinated by the NIST, and the inorganic exercises by the National Research Council (NRC) of Canada. Details of quality assurance for the Benthic Surveillance Program can be found in Lauenstein and Cantillo, 1993.
The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples and analyzing standard reference materials. To assess the reproducibility of results every tissue sample was analyzed in duplicate.
1
The primary collection apparatus was Otter trawls. Occasionally, fish were taken with hook and line, or with seine nets. These alternate collection methods were necessary because larger fish, such as older Atlantic croaker, were able to avoid an Otter trawl, or were found in untrawlable habitats such as shallow water, along marsh edges, and over oyster reefs. Fish liver tissue samples were analyzed for DNA-xenobiotic adducts in liver via Phosphorus 32-postlabeling assay to indicate genotoxic exposure. The analysis of DNA adducts by Phosphorus 32-postlabeling is a multistep process involving a series of biochemical reactions. First, DNA is hydrolyzed enzymatically to 3'monophosphates. The digest is then enriched in xenobiotic-modified mononucleotides by the selective removal of normal nucleotides. Following the enrichment step the adducted DNA is enzymatically labeled at the 5'-hydroxyl position with [Phosphorus 32] phosphate to form [5'-Phosphorus 32] deoxyribonucleoside 3',5'-bisphosphates. Separation of the Phosphorus 32-labeled adducts is usually accomplished by two-dimensional, thin-layer chromatography (TLC) on polyethyleneimine (PEI)-modified cellulose sheets. Autoradiography or storage phosphor imaging (Reichert et al., 1992) is then used to locate the radiolabeled adducts on the chromatogram (Reichert and French, 1994).The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples and analyzing standard reference materials.
1991-01-01T00:00:00
41238
Entity
Dataset Parameters for Benthic Surveillance DNA Xenobiotic Adducts data
gov.noaa.nmfs.inport:39258
Tyler Christensen
2017-04-05T12:51:32
SysAdmin InPortAdmin
2023-10-17T16:12:11
2018-02-08
National Centers for Coastal Ocean Science
NCCOS
1002
Public
No
2018-02-08
1 Year
2019-02-08