54574
Developing DNA Methods to Monitor Invasive Species and Biodiversity in Estuaries - NERRS/NSC(NERRS Science Collaborative)
Data Set
Published / External
47021
NERRS
Project
Completed
This project tested the use of eDNA methods to identify fish communities, assess biodiversity, and detect invasive crabs in different types of estuaries and connected streams. Samples were collected at Apalachicola, Great Bay, He’eai, Hudson, South Slough and Wells Reserves in 2018 and 2019. The project deployed two methods to analyze DNA in water samples: metabarcoding that identifies a wide range of species, and digital droplet Polymerase Chain Reaction (ddPCR) that identifies a single target organism. The team conducted baseline community assessments for fish at five reserve sites and conducted a survey of anadromous fish in a tributary stream at the sixth reserve. The project found that fish community and biodiversity assessments are well suited to eDNA applications, while invasive crabs are much harder to detect because they
do not shed much DNA. The project developed protocols and recommendations for the collection, filtering, and extraction of eDNA samples at estuarine sites, and provided information which will support the design of sampling programs for fish communities.
Biological monitoring programs are essential foundations for effective management of estuaries and coasts, but they can be expensive to conduct and methods such as the capture of living organisms may be traumatic for some target species. Advancements in DNA methods now make it possible to identify the organisms in an area by the DNA they leave behind, but there are relatively few proven sampling protocols and example datasets from estuaries. This project established a Collaborative Learning Community of Practice around Environmental DNA (eDNA) sampling within the National Estuarine Research Reserve system and explored the potential for eDNA to support estuarine management. Scientists and staff from six reserves worked Dataset Description: eDNA data from 6 NERRs NERRS Science Collaborative collaboratively with researchers, resource agencies, and a technical advisory team to design and
implement eDNA-based monitoring protocols for specific issues and species of interest in estuarine ecosystems.
Project Lead
Alison Watts
University of New Hampshire
(603) 862-0585
alison.watts@unh.edu
Project Type
Collaborative Research – generating science that informs decisions
==Project Partners
• Great Bay National Estuarine Research Reserve, New Hampshire
• New Hampshire Fish and Game Department
• Oregon Department of Fish and Wildlife
• South Slough National Estuarine Research Reserve, Oregon
• Wells National Estuarine Research Reserve, Maine
==About the data
The project team has made available three types data related to eDNA sequences and extractions from Wells, Great Bay, Hudson, Apalachicola, South Slough, and He’eia National Estuarine Research Reserves.
12S sequence data and summary of fish species
18S sequence data
Archived DNA extractions from water samples from 6 reserves
Theme
Global Change Master Directory (GCMD) Science Keywords
EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > ESTUARY
Spatial
Global Change Master Directory (GCMD) Location Keywords
CONTINENT > NORTH AMERICA > UNITED STATES OF AMERICA > MAINE
Spatial
Global Change Master Directory (GCMD) Location Keywords
CONTINENT > NORTH AMERICA > UNITED STATES OF AMERICA > NEW HAMPSHIRE
Spatial
Global Change Master Directory (GCMD) Location Keywords
CONTINENT > NORTH AMERICA > UNITED STATES OF AMERICA > OREGON
Theme
12S sequence data
Theme
18S sequence data
Theme
climate change
Theme
eDNA
Theme
environmental DNA
Theme
invasive species
Theme
monitoring
Spatial
Great Bay NERR, NH
Spatial
South Slough NERR, OR
Spatial
Wells NERR, ME
Data Set
None Planned
The distributor does not assume liability.
Metadata Contact
2017-10-31
Person
Cothran, Jeremy
jeremy.cothran@gmail.com
Originator
2017-11
2019
Person
Watts, Alison
alison.watts@unh.edu
Ground Condition
-70.97
-70.82
43.18
42.975
Great Bay, NH NERR
-70.6
-70.535
43.35
43.26
Wells, ME NERR
-124.344
-124.265
43.315
43.263
South Slough, OR NERR
Range
2017-11-01
2019-10-01
Unclassified
None
Cite this dataset when used as a source: NOAA retains the right to analyze, synthesize and publish summaries of the NERRS/NSC data. The NERRS/NSC retains the right to be fully credited for having collected and process the data. Following academic courtesy standards, the NERR site where the data were collected should be contacted and fully acknowledged in any subsequent publications in which any part of the data are used. The data enclosed within this package/transmission are only as accurate as the quality assurance and quality control procedures that are described in the associated metadata reporting statement allow. The user bears all responsibility for its subsequent use/misuse in any further analyses or comparisons. The Federal government does not assume liability to the Recipient or third persons, nor will the Federal government reimburse or indemnify the Recipient for its liability due to any losses resulting in any way from the use of this data. Requested citation format: NOAA National Estuarine Research Reserve System (NERRS) Science Collaborative(NSC).
http://www.nerrssciencecollaborative.org/project/Watts17
Online Resource
This site provides a project overview and links to all associated products, including data.
https://estuarydna.org
Online Resource
Processed datasets, data visualization tools, and related resources are available at the project webpage.
https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA667067
Online Resource
All genetic sequences generated by this project have been archived and made publicly available at the National Center for Biotechnology Information.
gov.noaa.nmfs.inport:54574
Jeremy Cothran
2018-11-01T11:20:13
SysAdmin InPortAdmin
2022-08-09T17:11:56
2020-03-11
Office for Coastal Management
OCM
1002
Public
No
2020-03-11
1 Year
2021-03-11