54712
Cell enumeration results from water sample analysis for three Azadinium species as well as the family Amphidomataceae determinted by real-time quantitative PCR using TaqMan probe assays from the coast of Washington and Puget Sound from 2016-02-03 to 2016-12-20
AZA Project Seawater Date for Lipophilic Toxins In Washington State 2016
Entity
Published / External
30880
Lipophilic Toxins in WA - Clear and present danger: monitoring and management of lipophilic shellfish toxins in Washington State
Data Set
Planned
Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample.
Spreadsheet
Yes
PARR
Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample.
1
SITE
TEXT
No
No
Active
The name or identification number of a given site at which samples were collected.
TEXT
As provided
2
DATE
DATE
No
No
Active
The date (local time) on which as sample was collected.
DATE
As provided
3
TIME
TIME
No
No
Active
The time (local time) on the date in which a sample began to be collected.
TIME
As provided
4
SAMPLEID
TEXT
No
No
Active
The name or identification number of a individual samples collected at specific sites. IDs incorporate a 2 letter site code followed by the eight digit sampling date and a 2 digit code signifying the sample type. May be followed by an S (surface) or I (3m depth integrated).
TEXT
As provided
5
LATITUDE
NUMBER
No
No
Active
The latitude of the sampling site in decmal degrees. Units for values are decimal degrees.
NUMBER
decimal degrees
As provided
6
LONGITUDE
NUMBER
No
No
Active
The longitude of the sampling site in decimal degrees. Units for values are decimal degrees.
NUMBER
decimal degrees
As provided
7
Other Azadinium or Amphidoma detected
TEXT
No
No
Active
Indicates whether or not other Azadinium species may be present in a water sample. This result is based on a genus specific quantitative PCR assay that is run if no Azadinium species are detected with species specific quantitative PCR assays. Units for values are Yes or No indicated by a 1 for Yes and a 0 for No.
TEXT
Yes or No indicated by a 1 for Yes and a 0 for No
Blank, 0, or 1
8
A. spinosum (cells/L)
TEXT
No
No
Active
Enumeration of Azadinum spinosum in a water sample. Units for values are Cells per liter of seawater.
TEXT
Cells per liter of seawater
less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value
9
A. poporum (cells/L)
TEXT
No
No
Active
Enumeration of Azadinum poporum in a water sample. Units for values are Cells per liter of seawater.
TEXT
Cells per liter of seawater
less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value
10
A. obesum (cells/L)
TEXT
No
No
Active
Enumeration of Azadinum obesum in a water sample. Units for values are Cells per liter of seawater.
TEXT
Cells per liter of seawater
less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value
gov.noaa.nmfs.inport:54712
Jeffrey Cowen
2018-11-08T11:33:45
SysAdmin InPortAdmin
2022-08-09T17:11:42
2018-11-08
Northwest Fisheries Science Center
NWFSC
2725 Montlake Boulevard East
Seattle
WA
98112
USA
206-860-3200
http://www.nwfsc.noaa.gov
1001
Public
No
2018-11-08
1 Year
2019-11-08