54712 AZA Project Seawater Date for Lipophilic Toxins In Washington State 2016 Entity Published / External 30880 Lipophilic Toxins in WA - Clear and present danger: monitoring and management of lipophilic shellfish toxins in Washington State Data Set Planned Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. Spreadsheet Yes PARR Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. 1 SITE TEXT No No Active The name or identification number of a given site at which samples were collected. TEXT As provided 2 DATE DATE No No Active The date (local time) on which as sample was collected. DATE As provided 3 TIME TIME No No Active The time (local time) on the date in which a sample began to be collected. TIME As provided 4 SAMPLEID TEXT No No Active The name or identification number of a individual samples collected at specific sites. IDs incorporate a 2 letter site code followed by the eight digit sampling date and a 2 digit code signifying the sample type. May be followed by an S (surface) or I (3m depth integrated). TEXT As provided 5 LATITUDE NUMBER No No Active The latitude of the sampling site in decmal degrees. Units for values are decimal degrees. NUMBER decimal degrees As provided 6 LONGITUDE NUMBER No No Active The longitude of the sampling site in decimal degrees. Units for values are decimal degrees. NUMBER decimal degrees As provided 7 Other Azadinium or Amphidoma detected TEXT No No Active Indicates whether or not other Azadinium species may be present in a water sample. This result is based on a genus specific quantitative PCR assay that is run if no Azadinium species are detected with species specific quantitative PCR assays. Units for values are Yes or No indicated by a 1 for Yes and a 0 for No. TEXT Yes or No indicated by a 1 for Yes and a 0 for No Blank, 0, or 1 8 A. spinosum (cells/L) TEXT No No Active Enumeration of Azadinum spinosum in a water sample. Units for values are Cells per liter of seawater. TEXT Cells per liter of seawater less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value 9 A. poporum (cells/L) TEXT No No Active Enumeration of Azadinum poporum in a water sample. Units for values are Cells per liter of seawater. TEXT Cells per liter of seawater less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value 10 A. obesum (cells/L) TEXT No No Active Enumeration of Azadinum obesum in a water sample. Units for values are Cells per liter of seawater. TEXT Cells per liter of seawater less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value gov.noaa.nmfs.inport:54712 Jeffrey Cowen 2018-11-08T11:33:45 SysAdmin InPortAdmin 2022-08-09T17:11:42 2018-11-08 Northwest Fisheries Science Center NWFSC 2725 Montlake Boulevard East Seattle WA 98112 USA 206-860-3200 https://www.nwfsc.noaa.gov 1001 Public No 2018-11-08 1 Year 2019-11-08