64268
Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across Hawaiian Archipelago from 2010 to 2016
ARMS Sequencing - Hawaiian Archipelago
Data Set
Published / External
63296
ARMS
Project
In Work
2021-01-01
The data described here includes cytochrome oxidase I (COI) DNA metabarcoding data collected from Autonomous Reef Monitoring Structures (ARMS). ARMS were deployed by the NOAA Pacific Islands Fisheries Science Center (PIFSC), Ecosystem Sciences Division (formerly the Coral Reef Ecosystem Division) under the National Coral Reef Monitoring Program (NCRMP) at stationary climate monitoring sites and used to assess and monitor cryptic reef diversity in the Hawaiian Archipelago. Developed in collaboration with the Census of Marine Life (CoML) Census of Coral Reef Ecosystems (CReefs), ARMS were designed to mimic the structural complexity of a reef and attract/collect colonizing marine invertebrates. The key innovation of the ARMS method is that biodiversity is sampled over precisely the same surface area in the exact same manner.
These data were gathered at specific reef sites across the Hawaiian Archipelago. ARMS units were set-up, deployed and recovered as described in the ARMS record in the related items section below. After ARMS were disassembled, different size fractions of samples and plate scrapings were preserved in ethanol for metabarcoding.
Coral reefs are among the most biologically diverse, complex, and productive of ecosystems. The vast majority of coral reef biodiversity is made up of the small and cryptic organisms living unseen by most within the reef matrix. This hidden community, the cryptobiota, are a critical component of coral reef trophic dynamics and play an essential role in nutrient recycling that enable reefs to thrive in oligotrophic environments. Despite their ecological importance, the cryptobiota are often ignored because they live deep within the reef matrix and require significant taxonomic expertise and time to collect and identify. By leaving ARMS units on the benthos for over two-years to attract the colonization and establishment of the cryptobiota ARMS overcome extraction constraints and by integrating next-generation DNA sequencing into ARMS unit processing, taxonomic limitations are diminshed through the establishment of molecular operational taxonomic units (MOTU) - species proxies - based on DNA sequence similarities.
The National Coral Reef Monitoring Program (NCRMP) is a framework for conducting sustained observations of biological, climate, and socioeconomic indicators at 10 priority coral reefs across the U.S. and its territories. This integrated approach will consolidate monitoring of coral reefs under a uniform method in the Pacific, Atlantic, Caribbean, and the Gulf of Mexico for the first time. NCRMP is funded by the CRCP and supported by NOAA Fisheries, NOAA National Centers for Coastal Ocean Science (NCCOS), and many other partners. The Coral Reef Ecosystem Program (CREP) at NOAA Fisheries is leading biological monitoring in the U.S. Pacific Islands Region.
The biological component of NCRMP in the Pacific provides a triennial ecological characterization at a broad spatial scale of general reef condition for reef fishes, corals and benthic habitat (i.e., fish species composition/density/size, benthic cover, and coral density/size/condition). Each year, CREP scientists work closely with CRCP and local partners to collect biological data on fish populations and coral reef communities from strategically selected sites during Reef Assessment and Monitoring Program (RAMP) missions. Innovative analysis techniques are then used to develop products that give fellow scientists, managers, decision makers and the public a better understanding of a region’s resources and how they are changing over time.
Theme
ISO 19115 Topic Category
biota
Theme
ISO 19115 Topic Category
oceans
Theme
CRCP Project
743
Theme
CRCP Project
National Coral Reef Monitoring Program
Theme
CoRIS Discovery Thesaurus
DNA/RNA Sequencing
Theme
CoRIS Theme Thesaurus
EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment
Theme
CoRIS Theme Thesaurus
EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Autonomous Reef Monitoring Structure (ARMS)
Theme
CoRIS Theme Thesaurus
EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > In Situ Biological
Theme
CoRIS Theme Thesaurus
EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs > Coral Reef Ecology
Theme
CoRIS Theme Thesaurus
EARTH SCIENCE > Oceans > Marine Biology > Cryptobiota
Theme
CoRIS Theme Thesaurus
EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates
Theme
CoRIS Theme Thesaurus
EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates > Biodiversity
Theme
CoRIS Theme Thesaurus
EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates > Census
Theme
CoRIS Theme Thesaurus
EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates > Macroinvertebrates
Theme
NODC DATA TYPES THESAURUS
INVERTEBRATE SPECIES
Theme
NODC DATA TYPES THESAURUS
SPECIES IDENTIFICATION
Theme
NODC DATA TYPES THESAURUS
SPECIES IDENTIFICATION - COUNT
Theme
NODC PLATFORM NAMES THESAURUS
HI'IALAKAI
Theme
NODC PLATFORM NAMES THESAURUS
Oscar Elton Sette
Theme
NODC PROJECT NAMES THESAURUS
CORAL REEF STUDIES
Theme
NODC PROJECT NAMES THESAURUS
Coral Reef Conservation Program
Theme
NODC PROJECT NAMES THESAURUS
National Coral Reef Monitoring Program
Theme
NODC SUBMITTING INSTITUTION NAMES THESAURUS
US DOC; NOAA; NMFS; Pacific Islands Fisheries Science Center; Ecosystem Sciences Division; Coral Reef Ecosystem Program
Theme
CREP
Theme
CReef
Theme
Census of Marine Life
Theme
Coral Reef Ecosystem Program
Theme
PIFSC
Theme
Pacific Islands Fisheries Science Center
Theme
cytochrome oxidase I
Temporal
Triennial
Spatial
CoRIS Place Thesaurus
COUNTRY/TERRITORY > United States of America > Hawaii > Hawaii > Hawaii Island (19N155W0003)
Spatial
CoRIS Place Thesaurus
COUNTRY/TERRITORY > United States of America > Hawaii > Hawaii > Kauai Island (22N159W0001)
Spatial
CoRIS Place Thesaurus
COUNTRY/TERRITORY > United States of America > Hawaii > Honolulu > French Frigate Shoals (24N166W0001)
Spatial
CoRIS Place Thesaurus
COUNTRY/TERRITORY > United States of America > Hawaii > Honolulu > Kure Atoll (28N178W0001)
Spatial
CoRIS Place Thesaurus
COUNTRY/TERRITORY > United States of America > Hawaii > Honolulu > Lisianski Island (25N173W0001)
Spatial
CoRIS Place Thesaurus
COUNTRY/TERRITORY > United States of America > Hawaii > Honolulu > Northwestern Hawaiian Islands (28N178W0000)
Spatial
CoRIS Place Thesaurus
COUNTRY/TERRITORY > United States of America > Hawaii > Honolulu > Oahu (21N157W0003)
Spatial
CoRIS Place Thesaurus
COUNTRY/TERRITORY > United States of America > Hawaii > Honolulu > Pearl and Hermes Reef (27N176W0001)
Spatial
CoRIS Place Thesaurus
COUNTRY/TERRITORY > United States of America > Hawaii > Maui > Maui Island (20N156W0004)
Spatial
CoRIS Place Thesaurus
COUNTRY/TERRITORY > United States of America > Hawaiian Islands (21N157W0027)
Spatial
CoRIS Place Thesaurus
OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Hawaiian Islands (21N157W0027)
Spatial
CoRIS Place Thesaurus
OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Hawaiian Islands > Hawaii Island > Hawaii Island (19N155W0003)
Spatial
CoRIS Place Thesaurus
OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Hawaiian Islands > Kauai Island > Kauai Island (22N159W0001)
Spatial
CoRIS Place Thesaurus
OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Hawaiian Islands > Maui Island > Maui Island (20N156W0004)
Spatial
CoRIS Place Thesaurus
OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Hawaiian Islands > Oahu Island > Oahu (21N157W0003)
Spatial
CoRIS Place Thesaurus
OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Northwestern Hawaiian Islands (28N178W0000)
Spatial
CoRIS Place Thesaurus
OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Northwestern Hawaiian Islands > French Frigate Shoals (24N166W0001)
Spatial
CoRIS Place Thesaurus
OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Northwestern Hawaiian Islands > Kure Atoll (28N178W0001)
Spatial
CoRIS Place Thesaurus
OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Northwestern Hawaiian Islands > Lisianski Island (25N173W0001)
Spatial
CoRIS Place Thesaurus
OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Northwestern Hawaiian Islands > Pearl and Hermes Reef (27N176W0001)
Spatial
NODC SEA AREA NAMES THESAURUS
North Pacific Ocean
Spatial
NODC SEA AREA NAMES THESAURUS
Papahanaumokuakea Marine National Monument
Spatial
MHI
Spatial
Main Hawaiian Islands
Spatial
NWHI
Spatial
Northwestern Hawaiian Islands
Instrument
NODC INSTRUMENT TYPES THESAURUS
ARMS
Instrument
NODC INSTRUMENT TYPES THESAURUS
Autonomous Reef Monitoring Structures
Instrument
environmental DNA
Instrument
metabarcoding
Pacific Islands Fisheries Science Center
Honolulu
HI
USA
NOAA IRC
Data Set
Other
None Planned
These data are merged fasta files of the forward and reverse sequencing on a MiSeq platform and have been deposited into Genbank.
Fasta Files
Fasta files for each successfully sequenced ARMS fraction that underwent COI DNA metabarcoding. The Sequence Read Archive (SRA) associated with Genbank stores sequences data from next-generation technologies. The SRA access numbers to these data are XXXX-XXXXX and can be access at: https://www.ncbi.nlm.nih.gov/sra/
https://www.ncbi.nlm.nih.gov/sra/
https://www.fisheries.noaa.gov/inport/item/45792
While every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty.
PIFSC Ecosystem Sciences Division and partners, with support from the Census of Marine Life (2008-2010), NOAA's Ocean Acidification Program (2010-2012), the Coral Reef Conservation Program (2012-2018), and the National Science Foundation grant # OISE12343541.
64647
ARMS metabarcoding
Published / External
Other
This is a text file with sequence data.
1
This file only contains ARMS sequences
1
No
No
Active
A text file with sequence data.
Data Steward
2010
Person
Timmers, Molly A
molly.timmers@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5449
Email preferred
Distributor
2010
Organization
Pacific Islands Fisheries Science Center
PIFSC
pifsc.info@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
808-725-5360
https://www.pifsc.noaa.gov
Pacific Island Fisheries Science Center homepage
Online Resource
8:00 a.m. - 4:30 p.m.
Metadata Contact
2010
Person
Olenski, Brooke
brooke.olenski@noaa.gov
Email preferred
Originator
2010
Organization
Pacific Islands Fisheries Science Center
PIFSC
pifsc.info@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
808-725-5360
https://www.pifsc.noaa.gov
Pacific Island Fisheries Science Center homepage
Online Resource
8:00 a.m. - 4:30 p.m.
Point of Contact
2010
2021
Person
Timmers, Molly A
molly.timmers@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5449
Email preferred
Ground Condition
Hawaiian Archipelago including the main and Northwestern Hawaiian Islands.
-159.6802
-154.8176
22.1669
18.968567
Main Hawaiian Islands (MHI), including Hawaii, Kauai, Maui, and Oahu
-178.378433
-166.11682
28.416767
23.627917
Northwestern Hawaiian Islands (NWHI), including French Frigate, Kure, Lisianski, and Pearl & Hermes.
Range
2008-10-07
2013-09-13
HA1007
HA1305
ARMS across the Northwestern Hawaiian Islands, deployed in 2010 and recovered in 2013 (units at FFS-32, FFS-33, and FFS-35 were deployed in 2008)
Range
2010-10-11
2013-08-23
HA1008
HA1304
ARMS across the Main Hawaiian Islands, deployed in 2010 and recovered in 2013
Range
2013-08-03
2016-08-22
HA1304
HA1606
ARMS across the Main Hawaiian Islands, deployed in 2013 and recovered in 2016
Range
2013-09-06
2016-09-24
HA1305
HA1606
ARMS across the Northwestern Hawaiian Islands, deployed in 2013 and recovered in 2016
Geographic 2D
EPSG:4326
WGS 84
World Geodetic System 1984
WGS 84
6378137
298.257223563
1
Geodetic Lattitude
Lat
degree
north
2
Geodetic Longitude
Lon
degree
east
Unclassified
Not applicable
Not applicable
NOAA Ecosystem Science Division (EDS) Data Sharing Recommendations, version 9.0 updated August 12, 2015:
ESD welcomes the opportunity to collaborate on research issues contributing to the scientific basis for better management of marine ecosystems. ESD has a very diverse set of field activities that generates large volumes of data using an array of data collection protocols.
The following recommendations are for your consideration as you use this data:
1) Data analyses should take all field exigencies into account. The most effective way to do this would be active collaboration with ESD principal investigators.
2) In all presentations, product releases, or publications using data generated by ESD, proper acknowledgement of both ESD and the individuals responsible for data collection is expected. Citing the DOI (if available) is preferred, a non-DOI example is listed below.
3) If you collect or generate data for the same study areas, ESD requests that you share relevant information on complimentary data collections.
4) Those receiving data are strongly urged to inform the ESD Data Management Team of any errors and discrepancies that are discovered during the course of using these data. They are further urged to bring to the attention of the Team all problems and difficulties encountered in using these data. This information is necessary in order to improve the collections and to facilitate more efficient and economical data processing and retrieval. The users are asked to supply copies of any missing data that may be located, and to provide information as to significant subsets and special aggregations of data that are developed in using the material provided.
Example citation:
"This publication makes use of data products provided by the Ecosystem Science Division (ESD), Pacific Islands Fisheries Science Center (PIFSC), National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA), with funding support from the NOAA Coral Reef Conservation Program (CRCP) and the National Science Foundation grant #OISE1243541. The analysis and interpretations presented here are solely that of the current authors.”
Data can be accessed online via Genbank.
None
Please cite PIFSC Ecosystem Sciences Division when using the data.
Suggested citation:
Ecosystem Sciences Division, Pacific Islands Fisheries Science Center, 2020: Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across the Hawaiian Archipelago from 2012 to 2015, https://www.fisheries.noaa.gov/inport/item/64268.
None
None
2021-01-01
Illumina Sequences
Illumina sequences are available in NCBI SRA (Sequence Read Archive) for each successfully sequenced fraction for each ARMS unit. Metabarcoding data was submitted to Genbank via Genome - https://geome-db.org/ which tags and records metadata associated with sequence files.
fasta
ranges from 17 to 600 MB
https://naturalhistory.si.edu/research/global-arms-program
Global ARMS program
Online Resource
Smithsonian partnership - Global ARMS program
https://www.coris.noaa.gov/monitoring/
NOAA's National Coral Reef Monitoring Program
Online Resource
https://www.fisheries.noaa.gov/region/pacific-islands#science
NOAA Fisheries: Pacific Islands
Online Resource
Information on NOAA CRED's official website
Genbank SRA files:
Go to: https://www.ncbi.nlm.nih.gov/sra/
DNA metabarcoding has inevitable PCR amplification and sequencing biases associated with using universal primers that amplify widely divergent taxa, and the variable rates of evolution within and among phyla that affect sequence clustering with a fixed divergence thresholds. Thus, some organisms may not be represented in the DNA metabarcoding data even though they were present on the plates. See https://onlinelibrary.wiley.com/doi/10.1002/edn3.163 for more details.
Analytical results could be different depending on the bioinformatics used to process the raw sequencing data, however, the overall picture will most likely remain the same.
As stated in the Analytical Accuracy section, bias in the data may result in one's choice of tools to bioinformatically process sequencing data.
All ARMS units that are recovered are disassembled, photographed, and sorted by size. Genetic analysis of ARMS samples may exist for a subset of fractions and locations. Missing fractions/locations could be a result of: 1) ARMS that were not recovered due to logistical constraints of the mission; 2) ARMS that were not found when divers returned to the site; and 3) ARMS fractions that were unsuccessfully extracted, amplified, or sequenced.
ARMS sample biodiversity over precisely the same surface area in the exact same manner. Thus, the use of ARMS is a systematic, consistent, and comparable method for monitoring the cryptobiota community overtime. Three units are deployed at each site to allow for replicate measurements. Divers typically record ARMS metadata into the master Microsoft Access database within a few days of the field operations and/or ARMS processing. QA/QC procedures by the ARMS team and the Cruise Data Manager are typically completed during the field mission.
Yes
Unknown
Yes
No
Unknown
Other
Unknown
Autonomous Reef Monitoring Structures (ARMS) are assembled, deployed, recovered, and processed as in related items below. The sessile organisms and the 100 um and 500 um motile fractions undergo DNA metabarcoding using the COI gene as the amplicon marker.
1
ARMS Deployment -
ARMS platforms are deployed as described in detail in the related items section below.
Person
Timmers, Molly A
molly.timmers@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5449
2
ARMS Recovery and Processing -
ARMS units are recovered, initially processed, and documented as described in related items below.
When all of the plate layers in the ARMS unit have been photographed and set aside (in seawater), the seawater from the disassembly tub, photo tray, and rinse bucket is sieved through adjoining 2 mm and 500 um sieve pans and an attachable 100 um mesh hand net. Material collected in the 500 um sieve and 100 um net are bulk preserved into two separate jars. Jars are filled with EtOH and labeled accordingly. The preserved 500 and 100 um sample fractions undergo a decantation process at a later date prior to DNA metabarcoding. All plates from an individual ARMS unit are scrapped en masse. Once all plates have been scraped, all the scrapings are transferred into a blender (Brevill; BBL600XL). The scrapings are blended for 45-60 seconds on maximum power until the sample is homogenized. The sample is then transferred from the blender to a 40 um net. The sample in the net is rinsed with filtered (< 40 um) seawater until all discharge from net is clear (takes ~2 gal). Four ~10 ml samples are preserved in 50 ml falcon tubes with DMSO and 4 ~10 ml samples are preserved in 95% EtOH. These blended preserved samples undergo DNA metabarcoding. The remaining sample is stored in a sterile whirlpak at -20C. Between the processing of each ARMS unit the blender is rinsed in fresh water to remove any remaining homogenate. The blender is then placed in a 10% bleach solution for 15 minutes. Finally all parts thoroughly rinsed with DI water if available or fresh water.
Person
Timmers, Molly A
molly.timmers@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5449
3
Decantation of the 100 and 500 um fractions:
NOTE: All equipment used in this step that is not already sterile need to be rinsed with fresh water, placed in 10% bleach solution for 20 minutes (new every day), transferred to Milliq water for 20 minutes (bleach bucket that the milliq water will go in first, and rinse) and then place under a UV light for 20 minutes.
Wear gloves. Take sample out the freezer and let sit to defrost before decantation. Empty container(s) into a 1 L conical flask (that has been bleached, rinsed and UV sterilized). Fill the conical flask with ~300 ml of Milli-Q water. Seal neck of flask with parafilm and shake vigorously for 30 seconds. Make sure to hold the parafilm tightly in place with one hand and place the other hand on the base of the flask.
When finished, immediately carefully pour the liquid through the correct sieve (45 μm for 100- 500 μm fraction; 106 μm for 500 μm – 2 mm fraction) trying not to pour the more dense sediment into the sieve. Fill flask with another 300 ml of Milli-Q and repeat process. Do this 7 times. The aim is to remove all the less dense biological material from the flask through decantation, while keeping the more dense sediment in the flask.
Collect the material in the sieve and weigh it using a sterile spatula and falcon tube. Put exactly half of the material in a 50 ml falcon tube, fill the tube with ethanol and freeze as a back-up. Place the other half in a sterile mortar and use a pestle to crush the sample for 1 minute. Collect the sample in a 50 ml falcon tube, using a little ethanol to re-suspend it and fill the tube with 95 % ethanol. This sample is now ready for DNA extraction. Store samples at -20C until extraction.
Finally, collect the sediment left in the conical flask, using a little Milliq water and pour into the same sieve used in the previous step. Collect the material, weigh it and place in a 50ml falcon tube. Fill with ethanol and freeze at -20C.
Person
Timmers, Molly A
molly.timmers@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5449
4
For DNA extraction, amplification, and sequencing methods see- Timmers M, Vicente J, Webb M, Jury C, Toonen RJ (2020) Sponging up diversity: evaluating metabarcoding performance for a taxonomically challenging phylum within a complex cryptobenthic community. Environmental DNA. https://doi.org/10.1002/edn3.163
Person
Timmers, Molly A
molly.timmers@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5449
5
To obtain the sequencing data, go to XXXXXXX which takes you to the National Center for Biotechnology Information (NCBI) which hosts Genebank, a genetic sequence database collection of all publicly available DNA sequences. To download a sequence file, click on the SRA link associated with each entry. Click on the data access tab in the new link and select the highlighted name to download the sequence file.
Person
Timmers, Molly A
molly.timmers@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5449
6
Once sequences are downloaded, you may choose to conduct the bioinformatics in a numbers of ways based on your preference.
Person
Timmers, Molly A
molly.timmers@noaa.gov
1845 Wasp Blvd.
Honolulu
HI
96818
USA
(808)725-5449
Not Applicable
Not Applicable
64647
Entity
ARMS metabarcoding
64267
Data Set
Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across American Samoa from 2012 to 2015
Series
64269
Data Set
Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across Marianas Archipelago from 2011 to 2014
Series
64270
Data Set
Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across PRIA from 2011 to 2015
Series
64332
Data Set
Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed in Batangas, Philippines from 2012 to 2015
Series
37506
Data Set
National Coral Reef Monitoring Program: Assessing and Monitoring Cryptic Reef Diversity of Colonizing Marine Invertebrates using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across the Hawaiian Archipelago from 2010 to 2016
Series
61283
Data Set
The effects of elevated temperature and acidification on the biodiversity of coral reef cryptobenthic communities that recruited Autonomous Reef Monitoring Structures placed within mesocosms at the Hawaii Institute of Marine Biology between July 2016 and June 2018
Cross Reference
gov.noaa.nmfs.inport:64268
Brooke Olenski
2021-02-09T08:29:03
SysAdmin InPortAdmin
2023-05-30T18:10:26
2022-02-04
Pacific Islands Fisheries Science Center
PIFSC
1845 Wasp Blvd.
Honolulu
HI
96818
USA
808-725-5300
http://www.pifsc.noaa.gov
8:00 a.m. - 4:30 p.m.
1001
Public
No
2022-02-04
1 Year
2023-02-04