gov.noaa.nmfs.inport:38941
eng
UTF8
dataset
National Centers for Coastal Ocean Science
resourceProvider
NCCOS Scientific Data Coordinator
NCCOS.data@noaa.gov
pointOfContact
2024-02-29T00:00:00
ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data
ISO 19115-2:2009(E)
National Status and Trends: Contaminant body burdens and histopathology of fish and shellfish from Alaska
NPRB_2010-19
2011-06-15
publication
NOAA/NMFS/EDM
38941
https://www.fisheries.noaa.gov/inport/item/38941
WWW:LINK-1.0-http--link
Full Metadata Record
View the complete metadata record on InPort for more information about this dataset.
information
Piniak, Greg
greg.piniak@noaa.gov
principalInvestigator
https://coastalscience.noaa.gov/projects/detail?key=232
WWW:LINK-1.0-http--link
Citation URL
Online Resource
download
http://www2.coastalscience.noaa.gov/publications/ccma/detail.aspx?resource=kXUVxxA8LH64JetFFum2pA6CRM0NB9yNGhLpzqLyij4=
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Citation URL
Online Resource
download
https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
WWW:LINK-1.0-http--link
Citation URL
Online Resource
download
tableDigital
Apeti, D.A., S.I. Hartwell, W.E. Johnson and G.G. Lauenstein. 2012. National Status and Trends Bioeffects Progam: Field Methods. NOAA National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Asssessment. NOAA NCCOS Technical Memorandum 135. Silver Spring, MD.27 pp. Apeti, D.A., W.E. Johnson, K.L. Kimbrough, and G.G. Lauenstein. 2012. National Status and Trends Mussel Watch Proram: Sampling Methods 2012 Update. NOAA Technical Memorandum 134. NOAA National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Asssessment. Silver Spring, MD. 39 pp. Kim, Y., Ashton-Alcox, K.A. and Powell. E.N. 2006. Histological Techniques for Marine Bivalve Molluscs: Update. Silver Spring, MD. NOAA Technical Memorandum NOS NCCOS, 27. 76 pp. Meyers, T. R., and J. D. Hendricks. 1985. Histopathology. In G.M. Rand and S.R. Petrocelli (editors), Fundamentals of aquatic toxicology, p. 283-331. Hemisphere, Washington, DC. McDonald, S. J., D. S. Frank, J. A. Ramirez, B. Wang, and J. M. Brooks. 2006. Ancillary Methods of the National Status and Trends Program: Update 2000-2006, NOAA Technical Memorandum, NOS NCCOS 28. Lauenstein, G. G. and A. Y. Cantillo (1993) Sampling and analytical methods of the National Status and Trends Program National Benthic Surveillance and Mussel Watch Kimbrough K.L. and G.G. Lauenstein. 2006a. Organic Contaminant Analytical Methods of the National Status and Trends Program: 2000-2006. U.S. Dept. Comm. NOAA Technical Memorandum 30, NOS NCCOS, Silver Spring, MD. Kimbrough, K.L. and G.G. Lauenstein. 2006b. Trace Metal Analytical Methods of the National Status and Trends Program: 2000-2006. US Dept. Comm., NOAA Technical Memorandum 29, NOS NCCOS, Silver Spring, MD. Hartwell, S.I., A.D. Apeti,., L.W. Claflin, W.E. Johnson, and K.L. Kimbrough. 2009. Sediment Quality Triad Assessment in Kachemak Bay: Characterization of Soft Bottom Benthic Habitats and Contaminant Bioeffects Assessment. North Pacific Research Board Final Report 726, 138pp.
In response to the growing concerns among Chugach communities, contaminant body burden and histopathological condition of chum and sockeye salmon (Oncorhynchus keta and Oncorhynchus nerka) and the shellfish cockles and softshell clams (Clinocardium nuttallii and Mya arenaria) were assessed. The fish and shellfish were collected from traditional subsistence harvest areas in the vicinity of Nanwalek, Port Graham and Seldovia, AK, and were analyzed for trace metals and residues of organic contaminants. Additionally, the fish and shellfish were histologically characterized for the presence, prevalence and severity of tissue pathology, disease, and parasite infections. Data served under this project include measurements of trace elements, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls, DDTs, chlorinated hydrocarbons and histopathology parameters, which include an array of about 30 parasitic taxa (e.g. bucephalus, chlamydia, ciliates, cestodes and nematodes) and 11 diseases (e.g. tumors, neoplasm and necrosis). This project provides invaluable baseline data that is georeferenced and served on the internet through the NOAA's National Status and Trends data portal.
Specific objectives of the study were to: the health risks associated with consumption of subsistence food items collected in the traditional harvest ground in the Chugach Native communities of south-central Alaska. To achieve this goal the following objectives were accomplished: 1) Characterize the potential contamination of sessile (shellfish) vs. mobile (salmon) components of the subsistence fishery, using body burdens and histopathology as metrics; 2) Assess any potential correlations between body burdens and histopathology data; 3) Evaluation of the health condition of the fish with analyses of tissue pathogens and occurrences of diseases.
The partners in the Resurrection Bay Interspecies Study were: (1) National Oceanographic and Atmospheric Administration (NOAA); (2) Chugach Regional Resource Commission(CRRC), (3) Alutiiq Pride Shellfish Hatchery.
completed
NCCOS Scientific Data Coordinator
NCCOS.data@noaa.gov
pointOfContact
NCCOS Scientific Data Coordinator
NCCOS.data@noaa.gov
custodian
notPlanned
Environmental Monitoring
theme
NOS Data Explorer Topic Category
DDT
NCCOS Research Data Type > Field Observation
NCCOS Research Priority > Long-term Monitoring
NCCOS Research Priority > Stressors, Impacts, Mitigation, and Restoration (SIMR)
NCCOS Research Topic > Bioeffects/Toxicity
NCCOS Research Topic > Chemical Contaminants
NCCOS Research Topic > Monitoring
PAH
PCB
TBT
analytes
bivalve mollusk
chemical
coastal ecosystem
coastal monitoring
contaminants
estuary
inorganic
organic
organochlorines
pesticides
polychlorinated biphenyls
polynuclear aromatic hydrocarbons
total butyltins
trace element
theme
Alaska
Kachemak Bay
Lower Cook Inlet
NCCOS Research Location > Geographic Area > Coastal Ocean
NCCOS Research Location > Region > Alaska
NCCOS Research Location > U.S. States and Territories > Alaska
Nanwalek
Port Graham
Seldovia
Southcentral Alaska
place
DOC/NOAA/NOS/NCCOS > National Centers for Coastal Ocean Science, National Ocean Service, NOAA, U.S. Department of Commerce
dataCentre
Global Change Master Directory (GCMD) Data Center Keywords
2017-04-24
publication
8.5
NSandT
project
InPort
otherRestrictions
Cite As: National Centers for Coastal Ocean Science, [Date of Access]: National Status and Trends: Contaminant body burdens and histopathology of fish and shellfish from Alaska [Data Date Range], https://www.fisheries.noaa.gov/inport/item/38941.
NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
otherRestrictions
Access Constraints: None
otherRestrictions
Use Constraints: NOAA requests that all individuals who download NOAA data acknowledge the source of these data in any reports, papers, or presentations. If you publish these data, please include a statement similar to: "Some or all of the data described in this article were produced by the NOAA's Ocean Service through its National Status and Trends Program (NSandT)"
otherRestrictions
Distribution Liability: These data were prepared by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, make any warranty, expressed or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed in this report, or represents that its use would not infringe privately owned rights. Reference therein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. Any views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. Although all data have been used by NOAA, no warranty, expressed or implied, is made by NOAA as to the accuracy of the data and/or related materials. The act of distribution shall not constitute any such warranty, and no responsibility is assumed by NOAA in the use of these data or related materials.
unclassified
NOAA Data Management Plan (DMP)
NOAA/NMFS/EDM
38941
https://www.fisheries.noaa.gov/inportserve/waf/noaa/nos/nccos/dmp/pdf/38941.pdf
WWW:LINK-1.0-http--link
NOAA Data Management Plan (DMP)
NOAA Data Management Plan for this record on InPort.
information
crossReference
eng; US
biota
environment
health
oceans
-151.9611
-151.6059
59.3386
59.487
Intertidal coastal areas off Port Graham, Nanwalek and Seldovia in Lower Cook Inlet
| Currentness: Ground Condition
2010
PRINCIPAL INVESTIGATORS: Dennis Apeti, NOAA Ocean Service, National Status and Trends Program; S. Ian Hartwell, NOAA Ocean Service, National Status and Trends Program; Mark Myers, NOAA National Marine Fisheries Service, Northwest Fisheries Science Center; Jeff Hetrick, Chugach Regional Resource Commission, Alvtiiq Pride Shellfish Hatchery SAMPLE COLLECTION INVESTIGATORS: Dennis Apeti, NOAA Ocean Service, National Status and Trends Program; S. Ian Hartwell, NOAA Ocean Service, National Status and Trends Program; Mark Myers, NOAA National Marine Fisheries Service, Northwest Fisheries Science Center; Jeff Hetrick, Chugach Regional Resource Commission, Alvtiiq Pride Shellfish Hatchery SAMPLES PROCESSING INVESTIGATORS: -Fish necropsis: Dennis Apeti, NOAA Ocean Service, National Status and Trends Program, S. Ian Hartwell, NOAA Ocean Service, National Status and Trends Program; Mark Myers, NOAA National Marine Fisheries Service, Northwest Fisheries Science Center Necropsis was conducted at the NOAA/UAF Kasitsna laboratory. http://www.nurp.noaa.gov/Spotlight/KasitsnaBay.htm. -Contaminant analyses: Organic and inorganic chemicals in fish and shellfish tissues: TDI Brooks Inc., College Station, TX. http://ccma.nos.noaa.gov/about/coast/nsandt/download.aspx. -Histopatology characterization in fish: Mark Myers, NOAA National Marine Fisheries Service, Northwest Fisheries Science Center. http://www.nwfsc.noaa.gov/. -Histopathology characterization in shellfish: Rutgers University, Haskin Shellfish Research Laboratory. http://hsrl.rutgers.edu/
false
eng
false
NPRB1019_Fish_Histopath2
2017-04-07
publication
NPRB1019_Shellfish_Histopath
2017-04-07
publication
NPRB1019_Contaminant
2017-04-07
publication
Text (Unstructured)
XML (Structured Text)
NCCOS Scientific Data Coordinator
NCCOS.data@noaa.gov
distributor
https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
WWW:LINK-1.0-http--link
https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Downloadable Data
download
https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
WWW:LINK-1.0-http--link
https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Downloadable Data
download
dataset
Accuracy
MEASUREMENT QUALITY OBJECTIVES: Sample collection followed established protocols for the National Status and Trends Program. The measurement quality objectives of NSandT specify accuracy and precision requirements of 30% for organic analytes and 15% for inorganic analytes in tissue samples. QA procedures include running blanks, spiked samples, and standard reference materials with each batch of samples. Any batch failing to meet the specifications is reanalyzed or rejected. The QA Criteria may be found in NOAAs Tech Memo NOS NCCOS 30 and 29. The standard NSandT analytical protocols are described in Kimbrough et al. (2006a and 2006b). The histological analyses of shellfish were performed at Rutgers University's Haskin Shellfish Laboratory. A detailed account of the protocol is described in the NOAA's NOS/NCCOS technical memorandum number 27 (Kim et al. 2006). From each site 5 individual organisms of legal harvestable size (38 mm) were randomly selected and prepared for the analysis. The animal was placed in Davidson's fixative for 1 week and then transferred to 70% alcohol for storage. For all bivalves, the fixed tissue samples were embedded in paraffin after dehydration and clearing. The tissue-paraffin block was then placed in a freezer overnight before sectioning. The paraffin-embedded tissue blocks were first sliced at 20 micrometers to expose an entire tissue cross-section, and then sectioned at 5 micrometers. Tissue sections were deparaffinized and hydrated using a xylene-ethanol series. Following hydration, slides were stained in a pentachrome series, dehydrated in a series of acetic acid dips followed by acetone, cleared in xylene and mounted in Permount. Each slide was examined microscopically. Shellfish histopathology characterization consisted of quantifying infection intensity of parasites, the occurrence and extent of tissue pathologies, and the intensity of diseases all of which were determined using quantitative or semi-quantitative measures. Fish histopathology analysis of salmon was performed at the NOAA's Environmental Conservation of the Northwest Fisheries Science Center, NOAA Fisheries, in Seattle, Washington. Histopathologic diagnosis on fish liver, head and trunk kidney, and gill tissues. Sections of liver, head and trunk kidney (1cm in thickness) and two gill arches collected from individual salmon will be preserved in Davidson's fixative. Lesions and parasites in tissue sections were identified and classified according to the criteria specified in comparative pathology (Meyers and Hendricks, 1985; Cotran et al., 1999)
Completeness Report
Data are believed to be complete
Conceptual Consistency
Sample collection followed established protocols for the National Status and Trends Program. The analytical instruments were calibrated by standard laboratory procedures. In total, approximately 5% of all analyses were QC analyses. Processing quality was considered acceptable if the following criteria were met: blanks were less than three times the minimum detection limit; accuracy, as determined by analysis of reference materials, was within 30% for organic analytes and within 20% for inorganic analytes; and precision, as determined by replicate analyses, was within 30% for organic analytes and within 20% for inorganic analytes. Additional specifications and guidelines are presented in Valente and Strobel (1993). Contact the project manager for additional information on the Quality Assurance Project Plan
Data Acquisition: The sample collection followed the standard Mussel Watch protocols. Sampling Objective: Fish (chum and sockeye salmon) and shellfish (softshell clams and cockles) were collected for the analysis of environmental contaminants and disease. Sampling Methods Summary: Sampling followed quality controlled and quality assured procedures of the NSandT and national Marine Fisheries Service (NMFS) for sample collection (Apeti et al., 2012; Cantillo and Lauenstein 1998; Meyers and Hendricks, 1985). The salmon and shellfish were collected at the traditional harvest grounds of Nanwalek, Port Graham and Seldovia. For clams and cockles, composites of 30 and 10 individual organisms were collected for the contamination and disease analyses respectively. For the salmon, the intent was to do inter-species comparison, but also investigated any gender differences. Thus, at each location, 10 (5 females and 5 males) specimen of each species were collected. Mollusks were not shucked in the field; cleaned samples were then packed on ice and shipped to the laboratory. Composite samples were prepared by homogenizing the soft parts. Fish necropsy occurred as soon after death of the salmon as possible. The fish were sorted by species and measured to assert proper age range. To prevent cross contamination during necropsy, four sets of dissection tools were used, each for the removal of fish muscle, liver, kidney and gill
2010-01-01T00:00:00
Source Contribution: Reference for histopathology chracterization standard operation procedure in shellfish | Source Geospatial Form: document | Type of Source Media: paper
Histological Techniques for Marine Bivalve Molluscs: Update
2006-01-01
publication
Kim, Y., Ashton-Alcox, K.A., Powell. E.N.
2006
Source Contribution: Reference for histopathology chracterization standard operation procedure in fish | Source Geospatial Form: document | Type of Source Media: paper
Histopathology
1985-01-01
publication
Meyers, T. R. and Hendricks, J. D.
1985
Source Contribution: Reference for standard operation procedure for organic contaminant analysis | Source Geospatial Form: document | Type of Source Media: online
Major and Trace Element Analytical Methods of the National Status and Trends Program: 2000-2006
2006-01-01
publication
Kimbrough, K.L. and Lauenstein, G.G.
http://ccma.nos.noaa.gov/publications/nsandtmethods.pdf
WWW:LINK-1.0-http--link
Source Citation URL
Source Citation URL
information
2000
2006
Source Contribution: Reference for standard operation procedure for sample collection | Source Geospatial Form: document | Type of Source Media: paper
National Status and Trends Bioeffects Program: Field Methods. NOAA National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment
2012-01-01
publication
Apeti, D.A., Hartwell, S.I., Johnson, W.E., Lauenstein, G.G.
Source Contribution: Reference for standard operation procedure for inorganic contaminants (heavy metals)analysis | Source Geospatial Form: document | Type of Source Media: online
Organic Contaminant Analytical Methods of the National Status and Trends Program: 2000-2006
2006-01-01
publication
Kimbrough K.L. and Lauenstein, G.G.
http://ccma.nos.noaa.gov/publications/organicsmethods.pdf
WWW:LINK-1.0-http--link
Source Citation URL
Source Citation URL
information
2000
2006
Source Contribution: Sampling and analytical methods used in this project followed the National Status and Trends standard field and analytical methods | Source Geospatial Form: document | Type of Source Media: paper
Sampling and Analytical Methods of the National Status and Trends Program Mussel Watch Project: 1993-1996 Update
1998-01-01
publication
Lauenstein, G.G., and Cantillo, A.Y.
1985
2012
Source Contribution: Reference for sampling standard operation procedure | Source Geospatial Form: document | Type of Source Media: paper
Sampling and Analytical Methods of the National Status and Trends Program Mussel Watch Project: 1993-1996 Update
1998-01-01
publication
Lauenstein, G.G., and Cantillo, A.Y.
http://ccma.nos.noaa.gov/publications/tm130.pdf
WWW:LINK-1.0-http--link
Source Citation URL
Source Citation URL
information
1993
1996