Cell enumeration results from water sample analysis for three Azadinium species as well as the family Amphidomataceae determinted by real-time quantitative PCR using TaqMan probe assays from the coast of Washington and Puget Sound from 2016-02-03 to 2016-12-20
Northwest Fisheries Science Center
Entity
(ENT)
| ID: 54712
| Published / External
Created: 2018-11-08
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Last Modified: 2022-08-09
Data Set (DS) | ID: 30880
ID: 54712
Entity (ENT)
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* » Title | Cell enumeration results from water sample analysis for three Azadinium species as well as the family Amphidomataceae determinted by real-time quantitative PCR using TaqMan probe assays from the coast of Washington and Puget Sound from 2016-02-03 to 2016-12-20 |
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Short Name | AZA Project Seawater Date for Lipophilic Toxins In Washington State 2016 |
* Status | Planned |
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* » Abstract |
Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. |
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• » Location Description |
Entity Information
Entity Type | Spreadsheet |
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Active Version? | Y |
Alias | |
Schema Name | PARR |
» Description |
Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. |
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Data Attributes
Attribute Summary
Score | Req'd? | PKey? | » Name | Data Storage Type | Description |
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100 | No | No | SITE | TEXT | The name or identification number of a given site at which samples were collected. |
100 | No | No | DATE | DATE | The date (local time) on which as sample was collected. |
100 | No | No | TIME | TIME | The time (local time) on the date in which a sample began to be collected. |
100 | No | No | SAMPLEID | TEXT | The name or identification number of a individual samples collected at specific sites. IDs incorporate a 2 letter site code followed by the eight digit sampling date and a 2 digit code signifying the sample type. May be followed by an S (surface) or I (3m depth integrated). |
100 | No | No | LATITUDE | NUMBER | The latitude of the sampling site in decmal degrees. Units for values are decimal degrees. |
100 | No | No | LONGITUDE | NUMBER | The longitude of the sampling site in decimal degrees. Units for values are decimal degrees. |
100 | No | No | Other Azadinium or Amphidoma detected | TEXT | Indicates whether or not other Azadinium species may be present in a water sample. This result is based on a genus specific quantitative PCR assay that is run if no Azadinium species are detected with species specific quantitative PCR assays. Units for values are Yes or No indicated by a 1 for Yes and a 0 for No. |
100 | No | No | A. spinosum (cells/L) | TEXT | Enumeration of Azadinum spinosum in a water sample. Units for values are Cells per liter of seawater. |
100 | No | No | A. poporum (cells/L) | TEXT | Enumeration of Azadinum poporum in a water sample. Units for values are Cells per liter of seawater. |
100 | No | No | A. obesum (cells/L) | TEXT | Enumeration of Azadinum obesum in a water sample. Units for values are Cells per liter of seawater. |
Attribute Details
Attribute Name | SITE |
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Seq. Order | 1 |
Data Storage Type | TEXT |
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Required | No |
Primary Key | No |
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Status | Active |
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The name or identification number of a given site at which samples were collected. |
General Data Type | TEXT |
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Allowed Values | As provided |
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Attribute Name | DATE |
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Seq. Order | 2 |
Data Storage Type | DATE |
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Required | No |
Primary Key | No |
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Status | Active |
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The date (local time) on which as sample was collected. |
General Data Type | DATE |
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Allowed Values | As provided |
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Attribute Name | TIME |
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Seq. Order | 3 |
Data Storage Type | TIME |
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Required | No |
Primary Key | No |
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Status | Active |
Description |
The time (local time) on the date in which a sample began to be collected. |
General Data Type | TIME |
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Allowed Values | As provided |
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Attribute Name | SAMPLEID |
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Seq. Order | 4 |
Data Storage Type | TEXT |
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Required | No |
Primary Key | No |
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Status | Active |
Description |
The name or identification number of a individual samples collected at specific sites. IDs incorporate a 2 letter site code followed by the eight digit sampling date and a 2 digit code signifying the sample type. May be followed by an S (surface) or I (3m depth integrated). |
General Data Type | TEXT |
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Allowed Values | As provided |
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Attribute Name | LATITUDE |
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Seq. Order | 5 |
Data Storage Type | NUMBER |
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Min Length | |
Required | No |
Primary Key | No |
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Scale | |
Status | Active |
Description |
The latitude of the sampling site in decmal degrees. Units for values are decimal degrees. |
General Data Type | NUMBER |
Unit of Measure | decimal degrees |
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Allowed Values | As provided |
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Attribute Name | LONGITUDE |
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Seq. Order | 6 |
Data Storage Type | NUMBER |
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Required | No |
Primary Key | No |
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Status | Active |
Description |
The longitude of the sampling site in decimal degrees. Units for values are decimal degrees. |
General Data Type | NUMBER |
Unit of Measure | decimal degrees |
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Allowed Values | As provided |
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Attribute Name | Other Azadinium or Amphidoma detected |
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Seq. Order | 7 |
Data Storage Type | TEXT |
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Required | No |
Primary Key | No |
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Status | Active |
Description |
Indicates whether or not other Azadinium species may be present in a water sample. This result is based on a genus specific quantitative PCR assay that is run if no Azadinium species are detected with species specific quantitative PCR assays. Units for values are Yes or No indicated by a 1 for Yes and a 0 for No. |
General Data Type | TEXT |
Unit of Measure | Yes or No indicated by a 1 for Yes and a 0 for No |
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Null Value Meaning | |
Allowed Values | Blank, 0, or 1 |
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Attribute Name | A. spinosum (cells/L) |
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Seq. Order | 8 |
Data Storage Type | TEXT |
Max Length | |
Min Length | |
Required | No |
Primary Key | No |
Precision | |
Scale | |
Status | Active |
Description |
Enumeration of Azadinum spinosum in a water sample. Units for values are Cells per liter of seawater. |
General Data Type | TEXT |
Unit of Measure | Cells per liter of seawater |
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Null Value Meaning | |
Allowed Values | less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value |
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Attribute Name | A. poporum (cells/L) |
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Seq. Order | 9 |
Data Storage Type | TEXT |
Max Length | |
Min Length | |
Required | No |
Primary Key | No |
Precision | |
Scale | |
Status | Active |
Description |
Enumeration of Azadinum poporum in a water sample. Units for values are Cells per liter of seawater. |
General Data Type | TEXT |
Unit of Measure | Cells per liter of seawater |
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Allowed Values | less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value |
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Attribute Name | A. obesum (cells/L) |
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Seq. Order | 10 |
Data Storage Type | TEXT |
Max Length | |
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Required | No |
Primary Key | No |
Precision | |
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Status | Active |
Description |
Enumeration of Azadinum obesum in a water sample. Units for values are Cells per liter of seawater. |
General Data Type | TEXT |
Unit of Measure | Cells per liter of seawater |
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Null Value Meaning | |
Allowed Values | less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value |
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Catalog Details
Catalog Item ID | 54712 |
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Metadata Record Created By | Jeffrey W Cowen |
Metadata Record Created | 2018-11-08 11:33+0000 |
Metadata Record Last Modified By | SysAdmin InPortAdmin |
» Metadata Record Last Modified | 2022-08-09 17:11+0000 |
Metadata Record Published | 2018-11-08 |
Owner Org | NWFSC |
Metadata Publication Status | Published Externally |
Do Not Publish? | N |
Metadata Workflow State | Published / External |
Metadata Last Review Date | 2018-11-08 |
Metadata Review Frequency | 1 Year |
Metadata Next Review Date | 2019-11-08 |
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