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Cell enumeration results from water sample analysis for three Azadinium species as well as the family Amphidomataceae determinted by real-time quantitative PCR using TaqMan prob...

Entity (ENT)  |  Northwest Fisheries Science Center (NWFSC)
GUID: gov.noaa.nmfs.inport:54712  |  Updated: August 9, 2022  |  Published / External 
Summary
Item Identification
Entity Info
Data Attributes
Catalog Details

Summary

Abstract

Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample.

Description

Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample.

Entity Information

Entity Type
Spreadsheet

Data Attribute / Type Description
SITE
TEXT 		The name or identification number of a given site at which samples were collected.
DATE
DATE 		The date (local time) on which as sample was collected.
TIME
TIME 		The time (local time) on the date in which a sample began to be collected.
SAMPLEID
TEXT 		The name or identification number of a individual samples collected at specific sites. IDs incorporate a 2 letter site code followed by the eight digit sampling date and a 2 digit code signifying the sample type. May be followed by an S (surface) or I (3m depth integrated).
LATITUDE
NUMBER 		The latitude of the sampling site in decmal degrees. Units for values are decimal degrees.
LONGITUDE
NUMBER 		The longitude of the sampling site in decimal degrees. Units for values are decimal degrees.
Other Azadinium or Amphidoma detected
TEXT 		Indicates whether or not other Azadinium species may be present in a water sample. This result is based on a genus specific quantitative PCR assay that is run if no Azadinium species are detected with species specific quantitative PCR assays. Units for values are Yes or No indicated by a 1 for Yes and a 0 for No.
A. spinosum (cells/L)
TEXT 		Enumeration of Azadinum spinosum in a water sample. Units for values are Cells per liter of seawater.
A. poporum (cells/L)
TEXT 		Enumeration of Azadinum poporum in a water sample. Units for values are Cells per liter of seawater.
A. obesum (cells/L)
TEXT 		Enumeration of Azadinum obesum in a water sample. Units for values are Cells per liter of seawater.

Child Items

No Child Items for this record.

Contact Information

No contact information is available for this record.

Please contact the owner organization (NWFSC) for inquiries on this record.

Item Identification

Title: Cell enumeration results from water sample analysis for three Azadinium species as well as the family Amphidomataceae determinted by real-time quantitative PCR using TaqMan probe assays from the coast of Washington and Puget Sound from 2016-02-03 to 2016-12-20
Short Name: AZA Project Seawater Date for Lipophilic Toxins In Washington State 2016
Status: Planned
Abstract:

Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample.

Entity Information

Entity Type: Spreadsheet
Active Version?: Yes
Schema Name: PARR
Description:

Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample.

Data Attributes

Attribute Summary

Rubric Score Primary Key? Name Type Description
100
 SITE TEXT The name or identification number of a given site at which samples were collected.
100
 DATE DATE The date (local time) on which as sample was collected.
100
 TIME TIME The time (local time) on the date in which a sample began to be collected.
100
 SAMPLEID TEXT The name or identification number of a individual samples collected at specific sites. IDs incorporate a 2 letter site code followed by the eight digit sampling date and a 2 digit code signifying the sample type. May be followed by an S (surface) or I (3m depth integrated).
100
 LATITUDE NUMBER The latitude of the sampling site in decmal degrees. Units for values are decimal degrees.
100
 LONGITUDE NUMBER The longitude of the sampling site in decimal degrees. Units for values are decimal degrees.
100
 Other Azadinium or Amphidoma detected TEXT Indicates whether or not other Azadinium species may be present in a water sample. This result is based on a genus specific quantitative PCR assay that is run if no Azadinium species are detected with species specific quantitative PCR assays. Units for values are Yes or No indicated by a 1 for Yes and a 0 for No.
100
 A. spinosum (cells/L) TEXT Enumeration of Azadinum spinosum in a water sample. Units for values are Cells per liter of seawater.
100
 A. poporum (cells/L) TEXT Enumeration of Azadinum poporum in a water sample. Units for values are Cells per liter of seawater.
100
 A. obesum (cells/L) TEXT Enumeration of Azadinum obesum in a water sample. Units for values are Cells per liter of seawater.

Attribute Details

SITE

CC ID: 797632
Seq. Order: 1
Data Storage Type: TEXT
Required: No
Primary Key: No
Status: Active
Description:

The name or identification number of a given site at which samples were collected.
General Data Type: TEXT
Allowed Values: As provided

DATE

CC ID: 797633
Seq. Order: 2
Data Storage Type: DATE
Required: No
Primary Key: No
Status: Active
Description:

The date (local time) on which as sample was collected.
General Data Type: DATE
Allowed Values: As provided

TIME

CC ID: 797634
Seq. Order: 3
Data Storage Type: TIME
Required: No
Primary Key: No
Status: Active
Description:

The time (local time) on the date in which a sample began to be collected.
General Data Type: TIME
Allowed Values: As provided

SAMPLEID

CC ID: 797635
Seq. Order: 4
Data Storage Type: TEXT
Required: No
Primary Key: No
Status: Active
Description:

The name or identification number of a individual samples collected at specific sites. IDs incorporate a 2 letter site code followed by the eight digit sampling date and a 2 digit code signifying the sample type. May be followed by an S (surface) or I (3m depth integrated).
General Data Type: TEXT
Allowed Values: As provided

LATITUDE

CC ID: 797636
Seq. Order: 5
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

The latitude of the sampling site in decmal degrees. Units for values are decimal degrees.
General Data Type: NUMBER
Unit of Measure: decimal degrees
Allowed Values: As provided

LONGITUDE

CC ID: 797637
Seq. Order: 6
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

The longitude of the sampling site in decimal degrees. Units for values are decimal degrees.
General Data Type: NUMBER
Unit of Measure: decimal degrees
Allowed Values: As provided

Other Azadinium or Amphidoma detected

CC ID: 797638
Seq. Order: 7
Data Storage Type: TEXT
Required: No
Primary Key: No
Status: Active
Description:

Indicates whether or not other Azadinium species may be present in a water sample. This result is based on a genus specific quantitative PCR assay that is run if no Azadinium species are detected with species specific quantitative PCR assays. Units for values are Yes or No indicated by a 1 for Yes and a 0 for No.
General Data Type: TEXT
Unit of Measure: Yes or No indicated by a 1 for Yes and a 0 for No
Allowed Values: Blank, 0, or 1

A. spinosum (cells/L)

CC ID: 797639
Seq. Order: 8
Data Storage Type: TEXT
Required: No
Primary Key: No
Status: Active
Description:

Enumeration of Azadinum spinosum in a water sample. Units for values are Cells per liter of seawater.
General Data Type: TEXT
Unit of Measure: Cells per liter of seawater
Allowed Values: less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value

A. poporum (cells/L)

CC ID: 797640
Seq. Order: 9
Data Storage Type: TEXT
Required: No
Primary Key: No
Status: Active
Description:

Enumeration of Azadinum poporum in a water sample. Units for values are Cells per liter of seawater.
General Data Type: TEXT
Unit of Measure: Cells per liter of seawater
Allowed Values: less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value

A. obesum (cells/L)

CC ID: 797641
Seq. Order: 10
Data Storage Type: TEXT
Required: No
Primary Key: No
Status: Active
Description:

Enumeration of Azadinum obesum in a water sample. Units for values are Cells per liter of seawater.
General Data Type: TEXT
Unit of Measure: Cells per liter of seawater
Allowed Values: less than the limit of detection (BLD) and less than the limit of quantification (BLQ) to the measured value

Catalog Details

Catalog Item ID: 54712
GUID: gov.noaa.nmfs.inport:54712
Metadata Record Created By: Jeffrey W Cowen
Metadata Record Created: 2018-11-08 11:33+0000
Metadata Record Last Modified By: SysAdmin InPortAdmin
Metadata Record Last Modified: 2022-08-09 17:11+0000
Metadata Record Published: 2018-11-08
Owner Org: NWFSC
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Last Review Date: 2018-11-08
Metadata Review Frequency: 1 Year
Metadata Next Review Date: 2019-11-08