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Summary

Short Citation
Northwest Fisheries Science Center, 2024: Lipophilic Toxins in WA - Clear and present danger: monitoring and management of lipophilic shellfish toxins in Washington State, https://www.fisheries.noaa.gov/inport/item/30880.
Full Citation Examples

Abstract

Lipophilic shellfish toxins comprise an extensive suite of compounds including those associated with the human syndromes known as diarrhetic shellfish poisoning (DSP) and azaspiracid shellfish poisoning (AZP). As a result of recent bloom events and subsequent human intoxications in Washington State (USA) due to DSP, there is a critical and urgent need for State public health officials to be able to monitor and accurately quantify harmful algal bloom (HAB) species associated with DSP and azaspiracid shellfish poisoning (AZP) and their toxins. There is now evidence that lipophilic toxins associated with DSP and AZP are present in water and/or shellfish, including oysters and mussels from Puget Sound and razor clams from the WA coast.Tight partnership with WDOH, the SoundToxins program, Olympic Region Harmful Algal Blooms (ORHAB) partnership, and Puget Sound shellfish growers (including the Jamestown S’Klallam tribe and other tribal representatives) will facilitate the study of the seasonal variability of lipophilic toxins and toxin-producing species at 10 geographically-distinct sites within Washington State waters where seawater or shellfish have recently been contaminated with these toxins. Stakeholder support throughout the project will ensure the transition of this project to the State at the end of 3 years as we have successfully demonstrated with ORHAB. Implementing routine lipophilic biotoxin monitoring will be a critical first step towards ensuring public safety while also enabling Washington State shellfish growers to sell their product to the European Union once trade is re-established.

Contains information regarding toxin producing species and toxin measurements in shellfish and seawater samples. Also includes associated environmental data.

Distribution Information

  • Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample.

  • Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included.

  • Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample.

  • Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included.

Access Constraints:

NA

Contact Information

Point of Contact
Nicolaus G Adams
Nicolaus.Adams@noaa.gov
206-860-6787

Metadata Contact
Northwest Fisheries Science Center (NWFSC)
nmfs.nwfsc.metadata@noaa.gov
206-860-3200

Extents

Geographic Area 1

-122.3062° W, -122.3062° E, 47.6449° N, 47.6449° S

Various locations in WA

Time Frame 1
2016-06-06 - Present

Item Identification

Title: Lipophilic Toxins in WA - Clear and present danger: monitoring and management of lipophilic shellfish toxins in Washington State
Status: In Work
Publication Date: 2018-11-07
Abstract:

Lipophilic shellfish toxins comprise an extensive suite of compounds including those associated with the human syndromes known as diarrhetic shellfish poisoning (DSP) and azaspiracid shellfish poisoning (AZP). As a result of recent bloom events and subsequent human intoxications in Washington State (USA) due to DSP, there is a critical and urgent need for State public health officials to be able to monitor and accurately quantify harmful algal bloom (HAB) species associated with DSP and azaspiracid shellfish poisoning (AZP) and their toxins. There is now evidence that lipophilic toxins associated with DSP and AZP are present in water and/or shellfish, including oysters and mussels from Puget Sound and razor clams from the WA coast.Tight partnership with WDOH, the SoundToxins program, Olympic Region Harmful Algal Blooms (ORHAB) partnership, and Puget Sound shellfish growers (including the Jamestown S’Klallam tribe and other tribal representatives) will facilitate the study of the seasonal variability of lipophilic toxins and toxin-producing species at 10 geographically-distinct sites within Washington State waters where seawater or shellfish have recently been contaminated with these toxins. Stakeholder support throughout the project will ensure the transition of this project to the State at the end of 3 years as we have successfully demonstrated with ORHAB. Implementing routine lipophilic biotoxin monitoring will be a critical first step towards ensuring public safety while also enabling Washington State shellfish growers to sell their product to the European Union once trade is re-established.

Contains information regarding toxin producing species and toxin measurements in shellfish and seawater samples. Also includes associated environmental data.

Supplemental Information:

Subject to Public Access to Research Results (PARR): Yes

Keywords

Theme Keywords

Thesaurus Keyword
UNCONTROLLED
None Azadinium
None Azaspiracids
None biotoxins
None chemistry
None Diarrhetic shellfish toxins
None Dinophysis
None fishery-dependent
None marine toxin
None toxic effects

Spatial Keywords

Thesaurus Keyword
UNCONTROLLED
None NWFSC Montlake Laboratory
None Various locations in WA

Instrument Keywords

Thesaurus Keyword
UNCONTROLLED
None and Depth
None In-Situ Instrument - In-Situ Instrument (not specified)
None Sonde and Probe - Conductivity
None Temperature

Platform Keywords

Thesaurus Keyword
UNCONTROLLED
None Animal and Plant Collection Device
None Animal and Plant Collection Device - Plankton Collection Device - Plankton Net
None Water Sampler Bottle - Surface Sample Bottle

Physical Location

Organization: Northwest Fisheries Science Center
City: Seattle
State/Province: WA
Country: USA

Data Set Information

Data Set Scope Code: Data Set
Data Set Type: Access Database
Data Presentation Form: Table (digital)

Support Roles

Data Steward

CC ID: 849730
Date Effective From: 2015-10-01
Date Effective To:
Contact (Person): Adams, Nicolaus G
Address: 2725 Montlake Blvd East
Seattle, WA 98112-2097
Email Address: Nicolaus.Adams@noaa.gov
Phone: 206-860-6787
Fax: 206-860-3335

Distributor

CC ID: 849731
Date Effective From: 2015-10-01
Date Effective To:
Contact (Organization): Northwest Fisheries Science Center (NWFSC)
Address: 2725 Montlake Boulevard East
Seattle, WA 98112
USA
Email Address: nmfs.nwfsc.metadata@noaa.gov
Phone: 206-860-3200
URL: NWFSC Home

Metadata Contact

CC ID: 849729
Date Effective From: 2015-10-01
Date Effective To:
Contact (Organization): Northwest Fisheries Science Center (NWFSC)
Address: 2725 Montlake Boulevard East
Seattle, WA 98112
USA
Email Address: nmfs.nwfsc.metadata@noaa.gov
Phone: 206-860-3200
URL: NWFSC Home

Originator

CC ID: 849733
Date Effective From: 2015-10-01
Date Effective To:
Contact (Person): Adams, Nicolaus G
Address: 2725 Montlake Blvd East
Seattle, WA 98112-2097
Email Address: Nicolaus.Adams@noaa.gov
Phone: 206-860-6787
Fax: 206-860-3335

Point of Contact

CC ID: 849732
Date Effective From: 2015-10-01
Date Effective To:
Contact (Person): Adams, Nicolaus G
Address: 2725 Montlake Blvd East
Seattle, WA 98112-2097
Email Address: Nicolaus.Adams@noaa.gov
Phone: 206-860-6787
Fax: 206-860-3335

Extents

Extent Group 1

Extent Group 1 / Geographic Area 1

CC ID: 849741
W° Bound: -122.3062
E° Bound: -122.3062
N° Bound: 47.6449
S° Bound: 47.6449
Description

Various locations in WA

Extent Group 1 / Time Frame 1

CC ID: 849740
Time Frame Type: Continuing
Start: 2016-06-06

Extent Group 2

Extent Group 2 / Geographic Area 1

CC ID: 849744
W° Bound: -122.3062
E° Bound: -122.3062
N° Bound: 47.6449
S° Bound: 47.6449
Description

NWFSC Montlake Laboratory

Extent Group 2 / Time Frame 1

CC ID: 849743
Time Frame Type: Continuing
Start: 0015-12-30

Access Information

Security Class: Unclassified
Data Access Procedure:

At this time, contact the Data Manager for information on obtaining access to this data set. In the near future, the NWFSC will strive to provide all non-sensitive data resources as a web service in order to meet the NOAA Data Access Policy Directive (https://nosc.noaa.gov/EDMC/PD.DA.php).

Data Access Constraints:

NA

Distribution Information

Distribution 1

CC ID: 849735
Start Date: 2018-11-07
End Date: Present
Download URL: https://www.webapps.nwfsc.noaa.gov/apex/parr/aza_project_seawater_date_for_lipophilic_toxins_in_washington_state_2016/data/page/
Distributor: Northwest Fisheries Science Center (NWFSC) (2015-10-01 - Present)
File Name: AZA Project Seawater Date for Lipophilic Toxins In
Description:

Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample.

Distribution 2

CC ID: 849737
Start Date: 2018-11-07
End Date: Present
Download URL: https://www.webapps.nwfsc.noaa.gov/apex/parr/aza_project_shellfish_data_for_lipophilic_toxins_in_washington_state_2016/data/page/
Distributor: Northwest Fisheries Science Center (NWFSC) (2015-10-01 - Present)
File Name: AZA Project Shellfish Data for Lipophilic Toxins I
Description:

Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included.

Distribution 3

CC ID: 849734
Start Date: 2018-11-07
End Date: Present
Download URL: https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/aza_project_seawater_date_for_lipophilic_toxins_in_washington_state_2016
Distributor: Northwest Fisheries Science Center (NWFSC) (2015-10-01 - Present)
File Name: AZA Project Seawater Date for Lipophilic Toxins In
Description:

Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample.

Distribution 4

CC ID: 849736
Start Date: 2018-11-07
End Date: Present
Download URL: https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/aza_project_shellfish_data_for_lipophilic_toxins_in_washington_state_2016
Distributor: Northwest Fisheries Science Center (NWFSC) (2015-10-01 - Present)
File Name: AZA Project Shellfish Data for Lipophilic Toxins I
Description:

Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included.

URLs

URL 1

CC ID: 849738
URL: https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/104746
Name: Lipophilic Toxins in WA
URL Type:
Online Resource
File Resource Format: Web site
Description:

NWFSC Dataset Information page. Contains information regarding toxin producing species and toxin measurements in shellfish and seawater samples. Also includes associated environmental data.

Data Quality

Quality Control Procedures Employed:

These data were collected and processed in accordance with established protocols and best practices under the direction of the project’s Principal Investigator. Contact the dataset Data Manager for full QA/QC methodology.

Data Management

Have Resources for Management of these Data Been Identified?: No
Approximate Percentage of Budget for these Data Devoted to Data Management: 0
Do these Data Comply with the Data Access Directive?: No
Is Access to the Data Limited Based on an Approved Waiver?: No
If Distributor (Data Hosting Service) is Needed, Please Indicate: No
Approximate Delay Between Data Collection and Dissemination: 0 days
If Delay is Longer than Latency of Automated Processing, Indicate Under What Authority Data Access is Delayed:

No Delay

Actual or Planned Long-Term Data Archive Location: NCEI-MD
Approximate Delay Between Data Collection and Archiving: 365
How Will the Data Be Protected from Accidental or Malicious Modification or Deletion Prior to Receipt by the Archive?:

The Northwest Fisheries Science Center facilitates backup and recovery of all data and IT components which are managed by IT Operations through the capture of static (point-in-time) backup data to physical media. Once data is captured to physical media (every 1-3 days), a duplicate is made and routinely (weekly) transported to an offsite archive facility where it is maintained throughout the data's applicable life-cycle.

Lineage

Lineage Statement:

NA

Catalog Details

Catalog Item ID: 30880
GUID: gov.noaa.nmfs.inport:30880
Metadata Record Created By: Jeffrey W Cowen
Metadata Record Created: 2016-02-24 10:06+0000
Metadata Record Last Modified By: Brendan A Sylvander
Metadata Record Last Modified: 2023-02-15 17:25+0000
Metadata Record Published: 2019-06-04
Owner Org: NWFSC
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Last Review Date: 2019-06-04
Metadata Review Frequency: 1 Year
Metadata Next Review Date: 2020-06-04