Gene expression data - Development of methods to sterilize sablefish
Data Set (DS) | Northwest Fisheries Science Center (NWFSC)GUID: gov.noaa.nmfs.inport:17984 | Updated: August 9, 2022 | Published / External
Summary
Short Citation
Northwest Fisheries Science Center, 2024: Gene expression data - Development of methods to sterilize sablefish, https://www.fisheries.noaa.gov/inport/item/17984.
Full Citation Examples
Methods for reproductive sterilization are broadly needed in marine aquaculture due to biosafety concerns associated with escapement of farmed fish and potential genetic contamination of wild stocks. Current methods for sterilization have been primarily tested in freshwater fishes and are often ineffective, particularly in males. The proposed activity builds upon our recent success in producing the first-ever monosex female stocks of sablefish for aquaculture and seeks to develop eco-friendly, non-GMO methods for reproductive sterilization. We will use sablefish as our model marine species for sterilization and continue to collaborate with University of Maryland faculty with expertise in cutting-edge techniques for sterilization. Development of sustainable marine aquaculture is a high priority at the local (NWFSC), regional (WCR), and national levels (NOAA, DOC, Congress). Our sablefish sex control/sterilization project was also highlighted as a critical priority and outstanding area of research in the NOAA Aquaculture Science Review published 28 Feb 2019. We ultimately aim to transfer a sterilization technology to the US sablefish aquaculture industry and expand our methodology to other important species, with the goal of improving sustainability of marine aquaculture and mitigating impacts on the environment.
Data on the expression of various genes in the gonads of sablefish during sex differentiation are collected.
Distribution Information
No Distributions available.
Access Constraints:NA
Child Items
No Child Items for this record.
Contact Information
Point of Contact
Adam Luckenbach
Adam.Luckenbach@noaa.gov
206-860-3463
Metadata Contact
Northwest Fisheries Science Center (NWFSC)
nmfs.nwfsc.metadata@noaa.gov
206-860-3200
Extents
-122.3062° W,
-122.3062° E,
47.6449° N,
47.6449° S
Northwest Fisheries Science Center: Montlake lab
2010-05-01 - Present
Item Identification
| Title: | Gene expression data - Development of methods to sterilize sablefish |
|---|---|
| Short Name: | Gene expression data - Sablefish Gonadal Differentiation |
| Status: | In Work |
| Creation Date: | 2011-03-01 |
| Abstract: |
Methods for reproductive sterilization are broadly needed in marine aquaculture due to biosafety concerns associated with escapement of farmed fish and potential genetic contamination of wild stocks. Current methods for sterilization have been primarily tested in freshwater fishes and are often ineffective, particularly in males. The proposed activity builds upon our recent success in producing the first-ever monosex female stocks of sablefish for aquaculture and seeks to develop eco-friendly, non-GMO methods for reproductive sterilization. We will use sablefish as our model marine species for sterilization and continue to collaborate with University of Maryland faculty with expertise in cutting-edge techniques for sterilization. Development of sustainable marine aquaculture is a high priority at the local (NWFSC), regional (WCR), and national levels (NOAA, DOC, Congress). Our sablefish sex control/sterilization project was also highlighted as a critical priority and outstanding area of research in the NOAA Aquaculture Science Review published 28 Feb 2019. We ultimately aim to transfer a sterilization technology to the US sablefish aquaculture industry and expand our methodology to other important species, with the goal of improving sustainability of marine aquaculture and mitigating impacts on the environment. Data on the expression of various genes in the gonads of sablefish during sex differentiation are collected. |
| Purpose: |
Laboratory data |
| Notes: |
Loaded by batch 4271, 01-30-2013 19:28 |
| Supplemental Information: |
Subject to Public Access to Research Results (PARR): No: Data Not Yet Collected |
Keywords
Theme Keywords
| Thesaurus | Keyword |
|---|---|
| UNCONTROLLED | |
| PARR Exclusion | Obsolete |
| None | gonadal sex differentiation |
| None | reproductive biology |
| None | sablefish |
| None | sex control |
| None | sustainable aquaculture |
Spatial Keywords
| Thesaurus | Keyword |
|---|---|
| UNCONTROLLED | |
| None | Manchester Research Station |
| None | Northwest Fisheries Science Center |
Instrument Keywords
| Thesaurus | Keyword |
|---|---|
| UNCONTROLLED | |
| None | Quantitative PCR Machine |
Physical Location
| Organization: | Northwest Fisheries Science Center |
|---|---|
| City: | Seattle |
| State/Province: | WA |
| Country: | USA |
Data Set Information
| Data Set Scope Code: | Data Set |
|---|---|
| Data Set Type: | Spreadsheet |
| Data Presentation Form: | Table (digital) |
Support Roles
Data Steward
| Date Effective From: | 2015-01-01 |
|---|---|
| Date Effective To: | |
| Contact (Person): | Luckenbach, Adam |
| Address: |
2725 Montlake Blvd East Seattle, WA 98112 |
| Email Address: | Adam.Luckenbach@noaa.gov |
| Phone: | 206-860-3463 |
| Fax: | 206-860-3467 |
Distributor
| Date Effective From: | 2015-01-01 |
|---|---|
| Date Effective To: | |
| Contact (Organization): | Northwest Fisheries Science Center (NWFSC) |
| Address: |
2725 Montlake Boulevard East Seattle, WA 98112 USA |
| Email Address: | nmfs.nwfsc.metadata@noaa.gov |
| Phone: | 206-860-3200 |
| URL: | NWFSC Home |
Metadata Contact
| Date Effective From: | 2015-01-01 |
|---|---|
| Date Effective To: | |
| Contact (Organization): | Northwest Fisheries Science Center (NWFSC) |
| Address: |
2725 Montlake Boulevard East Seattle, WA 98112 USA |
| Email Address: | nmfs.nwfsc.metadata@noaa.gov |
| Phone: | 206-860-3200 |
| URL: | NWFSC Home |
Originator
| Date Effective From: | 2015-01-01 |
|---|---|
| Date Effective To: | |
| Contact (Person): | Luckenbach, Adam |
| Address: |
2725 Montlake Blvd East Seattle, WA 98112 |
| Email Address: | Adam.Luckenbach@noaa.gov |
| Phone: | 206-860-3463 |
| Fax: | 206-860-3467 |
Point of Contact
| Date Effective From: | 2015-01-01 |
|---|---|
| Date Effective To: | |
| Contact (Person): | Luckenbach, Adam |
| Address: |
2725 Montlake Blvd East Seattle, WA 98112 |
| Email Address: | Adam.Luckenbach@noaa.gov |
| Phone: | 206-860-3463 |
| Fax: | 206-860-3467 |
Extents
Extent Group 1
Extent Group 1 / Geographic Area 1
| W° Bound: | -122.3062 | |
|---|---|---|
| E° Bound: | -122.3062 | |
| N° Bound: | 47.6449 | |
| S° Bound: | 47.6449 | |
| Description |
Northwest Fisheries Science Center: Montlake lab |
|
Extent Group 1 / Time Frame 1
| Time Frame Type: | Continuing |
|---|---|
| Start: | 2010-05-01 |
Extent Group 2
Extent Group 2 / Geographic Area 1
| W° Bound: | -122.5547 | |
|---|---|---|
| E° Bound: | -122.5547 | |
| N° Bound: | 47.569 | |
| S° Bound: | 47.569 | |
| Description |
Manchester Research Station: Manchester lab |
|
Extent Group 2 / Time Frame 1
| Time Frame Type: | Continuing |
|---|---|
| Start: | 2010-05-01 |
Access Information
| Security Class: | Unclassified |
|---|---|
| Data Access Procedure: |
Contact Data Manager |
| Data Access Constraints: |
NA |
URLs
URL 1
| URL: | https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/10193 |
|---|---|
| Name: | Gene expression data |
| URL Type: |
Online Resource
|
| File Resource Format: | Web site |
| Description: |
NWFSC Dataset Information page. Data on the expression of various genes in the gonads of sablefish during sex differentiation are collected. |
Technical Environment
| Description: |
Spreadsheet |
|---|
Data Quality
| Accuracy: |
Medium |
|---|---|
| Quality Control Procedures Employed: |
Data undergo QA/QC based on results of negative controls and standard curves included in all assays. Data are typically normalized to an internal reference gene measured in the same biological samples. |
Data Management
| Have Resources for Management of these Data Been Identified?: | No |
|---|---|
| Approximate Percentage of Budget for these Data Devoted to Data Management: | 1 |
| Do these Data Comply with the Data Access Directive?: | Yes |
| Is Access to the Data Limited Based on an Approved Waiver?: | No |
| If Distributor (Data Hosting Service) is Needed, Please Indicate: | Yes |
| Approximate Delay Between Data Collection and Dissemination: | 180 |
| If Delay is Longer than Latency of Automated Processing, Indicate Under What Authority Data Access is Delayed: |
QA/QC and data normalization |
| Actual or Planned Long-Term Data Archive Location: | NCEI-MD |
| Approximate Delay Between Data Collection and Archiving: | 365 |
| How Will the Data Be Protected from Accidental or Malicious Modification or Deletion Prior to Receipt by the Archive?: |
The Northwest Fisheries Science Center facilitates backup and recovery of all data and IT components which are managed by IT Operations through the capture of static (point-in-time) backup data to physical media. Once data is captured to physical media (every 1-3 days), a duplicate is made and routinely (weekly) transported to an offsite archive facility where it is maintained throughout the data's applicable life-cycle. |
Lineage
| Lineage Statement: |
Raw data generated using instrument and subsequently analyzed/normalized using spreadsheet software |
|---|
Catalog Details
| Catalog Item ID: | 17984 |
|---|---|
| GUID: | gov.noaa.nmfs.inport:17984 |
| Metadata Record Created By: | Jeffrey W Cowen |
| Metadata Record Created: | 2013-01-30 19:28+0000 |
| Metadata Record Last Modified By: | SysAdmin InPortAdmin |
| Metadata Record Last Modified: | 2022-08-09 17:11+0000 |
| Metadata Record Published: | 2019-06-07 |
| Owner Org: | NWFSC |
| Metadata Publication Status: | Published Externally |
| Do Not Publish?: | N |
| Metadata Last Review Date: | 2019-06-07 |
| Metadata Review Frequency: | 1 Year |
| Metadata Next Review Date: | 2020-06-07 |