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Short Citation:
Northwest Fisheries Science Center, 2022: Gene expression data - Development of methods to sterilize sablefish, https://www.fisheries.noaa.gov/inport/item/17984.

Item Identification

Title: Gene expression data - Development of methods to sterilize sablefish
Short Name: Gene expression data - Sablefish Gonadal Differentiation
Status: In Work
Creation Date: 2011-03-01
Abstract:

Methods for reproductive sterilization are broadly needed in marine aquaculture due to biosafety concerns associated with escapement of farmed fish and potential genetic contamination of wild stocks. Current methods for sterilization have been primarily tested in freshwater fishes and are often ineffective, particularly in males. The proposed activity builds upon our recent success in producing the first-ever monosex female stocks of sablefish for aquaculture and seeks to develop eco-friendly, non-GMO methods for reproductive sterilization. We will use sablefish as our model marine species for sterilization and continue to collaborate with University of Maryland faculty with expertise in cutting-edge techniques for sterilization. Development of sustainable marine aquaculture is a high priority at the local (NWFSC), regional (WCR), and national levels (NOAA, DOC, Congress). Our sablefish sex control/sterilization project was also highlighted as a critical priority and outstanding area of research in the NOAA Aquaculture Science Review published 28 Feb 2019. We ultimately aim to transfer a sterilization technology to the US sablefish aquaculture industry and expand our methodology to other important species, with the goal of improving sustainability of marine aquaculture and mitigating impacts on the environment.

Data on the expression of various genes in the gonads of sablefish during sex differentiation are collected.

Purpose:

Laboratory data

Notes:

Loaded by batch 4271, 01-30-2013 19:28

Supplemental Information:

Subject to Public Access to Research Results (PARR): No: Data Not Yet Collected

Keywords

Theme Keywords

Thesaurus Keyword
UNCONTROLLED
PARR Exclusion Obsolete
None gonadal sex differentiation
None reproductive biology
None sablefish
None sex control
None sustainable aquaculture

Spatial Keywords

Thesaurus Keyword
UNCONTROLLED
None Manchester Research Station
None Northwest Fisheries Science Center

Instrument Keywords

Thesaurus Keyword
UNCONTROLLED
None Quantitative PCR Machine

Physical Location

Organization: Northwest Fisheries Science Center
City: Seattle
State/Province: WA
Country: USA

Data Set Information

Data Set Scope Code: Data Set
Data Set Type: Spreadsheet
Data Presentation Form: Table (digital)

Support Roles

Data Steward

CC ID: 854426
Date Effective From: 2015-01-01
Date Effective To:
Contact (Person): Luckenbach, Adam
Address: 2725 Montlake Blvd East
Seattle, WA 98112
Email Address: Adam.Luckenbach@noaa.gov
Phone: 206-860-3463
Fax: 206-860-3467

Distributor

CC ID: 854427
Date Effective From: 2015-01-01
Date Effective To:
Contact (Organization): Northwest Fisheries Science Center (NWFSC)
Address: 2725 Montlake Boulevard East
Seattle, WA 98112
USA
Email Address: nmfs.nwfsc.metadata@noaa.gov
Phone: 206-860-3200
URL: NWFSC Home

Metadata Contact

CC ID: 854425
Date Effective From: 2015-01-01
Date Effective To:
Contact (Organization): Northwest Fisheries Science Center (NWFSC)
Address: 2725 Montlake Boulevard East
Seattle, WA 98112
USA
Email Address: nmfs.nwfsc.metadata@noaa.gov
Phone: 206-860-3200
URL: NWFSC Home

Originator

CC ID: 854429
Date Effective From: 2015-01-01
Date Effective To:
Contact (Person): Luckenbach, Adam
Address: 2725 Montlake Blvd East
Seattle, WA 98112
Email Address: Adam.Luckenbach@noaa.gov
Phone: 206-860-3463
Fax: 206-860-3467

Point of Contact

CC ID: 854428
Date Effective From: 2015-01-01
Date Effective To:
Contact (Person): Luckenbach, Adam
Address: 2725 Montlake Blvd East
Seattle, WA 98112
Email Address: Adam.Luckenbach@noaa.gov
Phone: 206-860-3463
Fax: 206-860-3467

Extents

Extent Group 1

Extent Group 1 / Geographic Area 1

CC ID: 854433
W° Bound: -122.3062
E° Bound: -122.3062
N° Bound: 47.6449
S° Bound: 47.6449
Description

Northwest Fisheries Science Center: Montlake lab

Extent Group 1 / Time Frame 1

CC ID: 854432
Time Frame Type: Continuing
Start: 2010-05-01

Extent Group 2

Extent Group 2 / Geographic Area 1

CC ID: 854436
W° Bound: -122.5547
E° Bound: -122.5547
N° Bound: 47.569
S° Bound: 47.569
Description

Manchester Research Station: Manchester lab

Extent Group 2 / Time Frame 1

CC ID: 854435
Time Frame Type: Continuing
Start: 2010-05-01

Access Information

Security Class: Unclassified
Data Access Procedure:

Contact Data Manager

Data Access Constraints:

NA

URLs

URL 1

CC ID: 854430
URL: https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/10193
Name: Gene expression data
URL Type:
Online Resource
File Resource Format: Web site
Description:

NWFSC Dataset Information page. Data on the expression of various genes in the gonads of sablefish during sex differentiation are collected.

Technical Environment

Description:

Spreadsheet

Data Quality

Accuracy:

Medium

Quality Control Procedures Employed:

Data undergo QA/QC based on results of negative controls and standard curves included in all assays. Data are typically normalized to an internal reference gene measured in the same biological samples.

Data Management

Have Resources for Management of these Data Been Identified?: No
Approximate Percentage of Budget for these Data Devoted to Data Management: 1
Do these Data Comply with the Data Access Directive?: Yes
Is Access to the Data Limited Based on an Approved Waiver?: No
If Distributor (Data Hosting Service) is Needed, Please Indicate: Yes
Approximate Delay Between Data Collection and Dissemination: 180
If Delay is Longer than Latency of Automated Processing, Indicate Under What Authority Data Access is Delayed:

QA/QC and data normalization

Actual or Planned Long-Term Data Archive Location: NCEI-MD
Approximate Delay Between Data Collection and Archiving: 365
How Will the Data Be Protected from Accidental or Malicious Modification or Deletion Prior to Receipt by the Archive?:

The Northwest Fisheries Science Center facilitates backup and recovery of all data and IT components which are managed by IT Operations through the capture of static (point-in-time) backup data to physical media. Once data is captured to physical media (every 1-3 days), a duplicate is made and routinely (weekly) transported to an offsite archive facility where it is maintained throughout the data's applicable life-cycle.

Lineage

Lineage Statement:

Raw data generated using instrument and subsequently analyzed/normalized using spreadsheet software

Catalog Details

Catalog Item ID: 17984
GUID: gov.noaa.nmfs.inport:17984
Metadata Record Created By: Jeffrey W Cowen
Metadata Record Created: 2013-01-30 19:28+0000
Metadata Record Last Modified By: SysAdmin InPortAdmin
Metadata Record Last Modified: 2022-08-09 17:11+0000
Metadata Record Published: 2019-06-07
Owner Org: NWFSC
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Last Review Date: 2019-06-07
Metadata Review Frequency: 1 Year
Metadata Next Review Date: 2020-06-07