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Short Citation:
Northeast Fisheries Science Center, 2023: Experimental tests of the effects of OA on early life history of marine fishes, https://www.fisheries.noaa.gov/inport/item/27478.

Item Identification

Title: Experimental tests of the effects of OA on early life history of marine fishes
Short Name: effects of OA on early life history of marine fishes
Status: Completed
Abstract:

These data characterize treatments and outcomes in laboratory-based ocean acidification experiments conducted that the NOAA NEFSC Howard Laboratory. Experiments vary by species used, scope of study (experiment duration and species’ life-stages examined), and experimental design including number of treatments, levels, and replicates, and the suite of response variables. This experiment used summer flounder (Paralichthys dentatus) as subjects, one treatment (concentration of aqueous pCO2), three treatment levels (775, 1808, and 4714 uatm pCO2, pHs of 7.8, 7.5, and 7.1, respectively), three replicates (replicated downstream from each unique CO2 source), and the following response variables: survival of embryos to hatching and of larvae to age 28 d post-hatching; size, shape, and image-based developmental features of larvae; and histopathologically determined status of key organs (eye, heart, liver, gall bladder, gastro-intestinal tract, epidermis, kidney, spinal cord, and muscles) and cranio-facial and skeletal elements.

Purpose:

Contribution to ocean acidification knowledge regarding biological responses to elevated CO2. Supported by NOAA’s Ocean Acidification Program and the Northeast Fisheries Science Center.

Supplemental Information:

Chambers, R.C., A.C. Candelmo, E.A. Habeck, M.E. Poach, D. Wieczorek, K.R. Cooper, C.E. Greenfield, and B.A. Phelan. 2014. Effects of elevated CO2 in the early life stages of summer flounder, Paralichthys dentatus, and potential consequences of ocean acidification. Biogeosciences, 11, 1613-1626, doi:10.5194/bg-11-1613-2014.

Keywords

Theme Keywords

Thesaurus Keyword
UNCONTROLLED
None CO2
None development
None embryos
None growth
None larvae
None ocean acidification
None Paralichthys dentatus
None summer flounder
None survival

Spatial Keywords

Thesaurus Keyword
UNCONTROLLED
None Mid-Atlantic USA

Stratum Keywords

Thesaurus Keyword
UNCONTROLLED
None pelagic

Physical Location

Organization: NEFSC James J. Howard Lab
City: Highlands
State/Province: NJ
Country: USA

Data Set Information

Data Set Scope Code: Data Set
Data Set Type: MS Excel Spreadsheet
Maintenance Frequency: None Planned
Data Presentation Form: Table (digital)

Support Roles

Data Steward

CC ID: 247383
Date Effective From: 2014
Date Effective To:
Contact (Person): Chambers, Robert Christopher
Address: 74 Magruder Road
Highlands, NJ 07732-0428
USA
Email Address: chris.chambers@noaa.gov
Phone: 732-872-3075
Fax: 732-872-3088

Distributor

CC ID: 247391
Date Effective From: 2014
Date Effective To:
Contact (Person): Chambers, Robert Christopher
Address: 74 Magruder Road
Highlands, NJ 07732-0428
USA
Email Address: chris.chambers@noaa.gov
Phone: 732-872-3075
Fax: 732-872-3088

Metadata Contact

CC ID: 247384
Date Effective From: 2014
Date Effective To:
Contact (Person): Chambers, Robert Christopher
Address: 74 Magruder Road
Highlands, NJ 07732-0428
USA
Email Address: chris.chambers@noaa.gov
Phone: 732-872-3075
Fax: 732-872-3088

Originator

CC ID: 247392
Date Effective From: 2014
Date Effective To:
Contact (Person): Chambers, Robert Christopher
Address: 74 Magruder Road
Highlands, NJ 07732-0428
USA
Email Address: chris.chambers@noaa.gov
Phone: 732-872-3075
Fax: 732-872-3088

Point of Contact

CC ID: 247382
Date Effective From: 2014
Date Effective To:
Contact (Person): Chambers, Robert Christopher
Address: 74 Magruder Road
Highlands, NJ 07732-0428
USA
Email Address: chris.chambers@noaa.gov
Phone: 732-872-3075
Fax: 732-872-3088

Extents

Extent Group 1

Extent Group 1 / Geographic Area 1

CC ID: 247396
Description

New Jersey, USA coastal waters (source of adults used to produce the offspring used in these experiments

Extent Group 1 / Time Frame 1

CC ID: 247397
Time Frame Type: Range
Start: 2010
End: 2014
Description:

Experiments were conducted seasonally, following the availability of spawning products from the species and source populations. In this experiment, adults were collected during June – September 2011 and were spawned in December 2011. Embryos and larvae were maintained in the experiment through January 2012. Images from experiments were analyzed during 2012 and histopathology was performed during 2012-13.

Access Information

Security Class: Unclassified
Data Access Procedure:

NEFSC Data Access Procedure:

1. Formal request in writing usually to the data owner/contact or Center Director;

2. Requester is contacted by data owner to review and verify the request content and details for data delivery options.

3. If data are confidential then owner will determine if the data may be released to the requester;

4. If data can be released, the data are downloaded and packaged for delivery electronically; or the requester may be directed to where the data is available online.

Data Access Constraints:

NEFSC Data Access Constraint:

If data are stored on an internal NEFSC network, the requester may not have direct access to query the data unless they are also authorized for network access.

Distribution Information

Distribution 1

CC ID: 247407
Download URL: ftp://ftp.nefsc.noaa.gov/pub/dropoff/PARR/EAD/FEB/27478/
Distributor:
Description:

4 Excel Files:

27478_EGG_SURVIVAL.xls

27478_LARVAL_CF.xls

27478_LARVAL_SIZE.xls

27478_YSL_SURVIVAL.xls

Technical Environment

Description:

Data entered and saved in Microsoft Excel versions 2007, 2010. Data reside on network drive at location ‘R:\RESEARCH\OA - FY12\summer flounder’, and are backed up nightly on NEFSC Sandy Hook Lab servers.

Data Quality

Completeness Report:

Data provided are the individual-level observations prior to statistical reduction to means, principal components, etc. Data were not filtered or selected after check for errors.

Quality Control Procedures Employed:

All entered data were check for correspondence to original hand-written data sheets (where used). Data were inspected for statistical outliers via frequency plots and scatterplots. Some data were given subjective quality codes, e.g., based on quality / clarity of image used as the source of image-extraction software.

Data Management

Have Resources for Management of these Data Been Identified?: Yes
Approximate Percentage of Budget for these Data Devoted to Data Management: Unknown
Do these Data Comply with the Data Access Directive?: Yes
Is Access to the Data Limited Based on an Approved Waiver?: No
Approximate Delay Between Data Collection and Dissemination: none
Actual or Planned Long-Term Data Archive Location: Other
If World Data Center or Other, Specify: NEFSC Network
Approximate Delay Between Data Collection and Archiving: Unknown
How Will the Data Be Protected from Accidental or Malicious Modification or Deletion Prior to Receipt by the Archive?:

Archival of source data preserving unaltered collected data, Scheduled backups, Remote storage backups, Password protection

Lineage

Lineage Statement:

Data were collected by pre-planned design and varied with experiment. Data on embryo deaths and hatchlings were collected at a daily frequency; size and shape data of hatchlings (Nmax = 10 per replicate) were collected from the interquartile range of the hatch frequency distribution once hatching commenced; mortality of larvae was checked daily; larvae were sampled for image-based size and shape every 7 days through 28 days post-hatching. Data from images were scored via image analysis software (Image Tool v.3). Histopathology data were collected from preserved larvae (staining sequence in Chambers et al. 2014 ibid.). All data were entered into Excel spreadsheets.

Catalog Details

Catalog Item ID: 27478
GUID: gov.noaa.nmfs.inport:27478
Metadata Record Created By: Patricia L Jones
Metadata Record Created: 2015-09-25 11:13+0000
Metadata Record Last Modified By: SysAdmin InPortAdmin
Metadata Record Last Modified: 2022-08-09 17:11+0000
Metadata Record Published: 2016-06-28
Owner Org: NEFSC
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Last Review Date: 2016-06-28
Metadata Review Frequency: 1 Year
Metadata Next Review Date: 2017-06-28