AFSC/RACE/FBEP/Hurst: Effects of ocean acidification on hatch size and larval growth of walleye pollock (Theragra chalcogramma)

This dataset is from laboratory experiments that examined the direct effects of projected levels of ocean acidification on the eggs and larvae of walleye pollock.

The experiments that produced this dataset were designed to study how hatch size and larval growth rates of walleye pollock are affected by ocean acidification.

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See Hurst, T.P., Fernandez, E.R., Mathis, J.T., 2012. Effects of ocean acidification on hatch size and larval growth of walleye pollock (Theragra chalcogramma). ICES Journal of Marine Science, 70: 812-822

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Data quality was checked for hatch and size metrics, and for CO2 system measures.

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Egg incubation experiments: Eggs for the experiments were produced over three spawning seasons by a captive broodstock of walleye pollock at the AFSC laboratory in Newport, Oregon. Batches of eggs were collected from the spawning tanks periodically through the spawning period. From each egg batch, 3-4 replicate samples of 1.5-2mL of eggs were incubated in each of the four CO2 treatments. Each day during the hatch cycle, up to 20 newly hatched larvae from each beaker were digitally photographed under a dissecting microscope and measurements were taken with an image analysis system.

Larval growth experiments: Three independent larval rearing experiments were conducted with offspring from broodstock spawning events. For each experiment, eggs were collected over a 3-day spawning period. At the first feeding stage, 7 days post-hatch, larvae were transferred to three replicate rearing tanks maintained at each of the four CO2 treatment levels. A sample of 15-20 fish was drawn from each replicate tank at weekly intervals from the first feeding stage until fish reached 33-38 DPH. Sampled larvae were digitally photographed under a dissecting microscope and measurements were made and used to calculate larval condition index.