OBSOLETE: Coral genetic - Taxonomic and genetic identification of deep-sea corals in bycatch and field surveys -linked to groundfish survey dataset

Data for this project will reside in the West Coast Groundfish Bottom Trawl Survey Data Base. This proposal relates to a continuation and augmentation of our efforts in creating a DNA sequence repository and voucher collection of coral and sponge species found off the U.S. West Coast, and initial steps toward population-level analysis in deep-sea octocorals and sponges using microsatellite loci. We propose to continue taking genetic samples from specimens collected during the 2012 West Coast Groundfish Bottom Trawl Survey, and continue DNA sequencing of those specimens. We will then link the DNA data to our verified specimens to create a West Coast repository that will serve not only as an essential resource for coral researchers and other stakeholders, but also as a foundation for future research efforts at the NWFSC, aimed at understanding the biogeography and ecology of coral species in these deep-sea communities.

Our primary goals with this funding opportunity are to hire an NRC postdoctoral fellow to work full-time on this project, and to continue to support at-sea specimen collection.

Coral DNA represented by Data Set "Deep Sea Coral voucher sequence dataset".

At this time, contact the Data Manager for information on obtaining access to this data set. In the near future, the NWFSC will strive to provide all non-sensitive data resources as a web service in order to meet the NOAA Data Access Policy Directive (https://nosc.noaa.gov/EDMC/PD.DA.php).

NA

Sequencing quality checks: these data were collected and processed in accordance with established protocols and best practices under the direction of the projects Principal Investigator. Contact the dataset Data Manager for full QA/QC methodology.

No Delay

The Northwest Fisheries Science Center facilitates backup and recovery of all data and IT components which are managed by IT Operations through the capture of static (point-in-time) backup data to physical media. Once data is captured to physical media (every 1-3 days), a duplicate is made and routinely (weekly) transported to an offsite archive facility where it is maintained throughout the data's applicable life-cycle.

DNA extraction and sequencing, morphological identifications made, SEM images, QA/QC, phylogenetic analysis