Assessing cryptic reef diversity of colonizing marine invertebrates using Autonomous Reef Monitoring Structures (ARMS) deployed at coral reef sites in Batangas, Philippines from...
Data Set (DS) | Pacific Islands Fisheries Science Center (PIFSC)GUID: gov.noaa.nmfs.inport:45818 | Updated: October 3, 2024 | Published / External
Item Identification
Title: | Assessing cryptic reef diversity of colonizing marine invertebrates using Autonomous Reef Monitoring Structures (ARMS) deployed at coral reef sites in Batangas, Philippines from 2012 to 2015 |
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Short Name: | Philippines: ARMS |
Status: | Completed |
Publication Date: | 2017 |
Abstract: |
Autonomous Reef Monitoring Structures (ARMS) are used by the NOAA Coral Reef Ecosystem Program (CREP) to assess and monitor cryptic reef diversity across the Pacific. Developed in collaboration with the Census of Marine Life (CoML) Census of Coral Reef Ecosystems (CReefs), ARMS are designed to mimic the structural complexity of a reef and attract/collect colonizing marine invertebrates. The key innovation of the ARMS method is biodiversity is sampled over precisely the same surface area in the exact same manner. Thus, the use of ARMS is a systematic, consistent, and comparable method for monitoring the marine cryptobiota community over time. The data described here were collected by CREP from ARMS moored at fixed climate survey sites located on hard bottom shallow water (< 15 m) habitats in the Philippines. Climate sites were established by CREP to assess multiple features of the coral reef environment (in addition to the data described herein) from March 2012 to June 2015, and three ARMS units were deployed by SCUBA divers at each survey site. The data can be accessed online via the NOAA National Centers for Environmental Information (NCEI) Ocean Archive. Each ARMS unit, constructed in-house by CREP, consisted of 23 cm x 23 cm gray, type 1 PVC plates stacked in alternating series of 4 open and 4 obstructed layers and attached to a base plate of 35 cm x 45 cm, which was affixed to the reef. Upon recovery, each ARMS unit was encapsulated, brought to the surface, and disassembled and processed. Disassembled plates were photographed to document recruited sessile organisms and scraped clean and preserved in 95% ethanol for DNA processing. Recruited motile organisms were sieved into 3 size fractions: 2 mm, 500 µm, and 100 µm. The 500 µm and 100 µm fractions were bulked and also preserved in 95% ethanol for DNA processing. The 2 mm fraction was sorted into morphospecies. Once the data for DNA processing is published, it will be accessible in a separate record that will be added in the "Related Items" section below. |
Purpose: |
The Coral Reef Ecosystem Program (CREP) at NOAA Fisheries is conducting in-situ climate monitoring across the U.S. Pacific Islands Region. Climate monitoring provides a comprehensive view of climate change impacts on coral reef ecosystems and helps identify areas of resilience and vulnerability. The key indicators used to identify and monitor climate-driven trends include 1) thermal stress caused by changes in sea temperature, 2) ocean acidification resulting from changes in carbonate chemistry, and 3) ecological impacts by collecting data on coral growth rates and community structure to understand the impacts of thermal stress and ocean acidification on the ecosystem. This particular dataset for the Philippines is part of a 3-year project ("Climate, Biodiversity and Fisheries in the Coral Triangle: Embracing the E in Ecosystem Approaches to Fisheries Management") implemented by CREP. This project was funded by NOAA's Coral Reef Conservation Program (CRCP) and the U.S. Agency for International Development (USAID) Regional Development Mission Asia (RDMA) as part of the U.S. Coral Triangle Initiative, with additional support from the Coral Triangle Support Partnership and USAID Philippines. The goal of the project was to build on CREP's expertise to provide tools and information about climate change, ocean acidification, and their impacts on biodiversity and fisheries that could inform and be incorporated into an Ecosystem Approach to Fisheries Management (EAFM) for the Philippines. CREP worked with local governments, communities, and NGOs to build science capacity by establishing robust observing capabilities and providing hands-on training to initiate collection of climate science information for the Verde Island Passage in the Philippines that can be used toward adaptive EAFM. |
Supplemental Information: |
The NOAA Coral Reef Ecosystem Program (CREP) traveled to the Verde Island Passage region of the Philippines in 2012 to deploy moored oceanographic (subsurface temperature recorders [STRs]) and ecological (calcification accretion units [CAUs] and autonomous reef monitoring structures [ARMS]) instrumentation, to collect surface and bottom water samples, and to conduct benthic photo-quadrat surveys, which included the collection of benthic images; in 2013 to collect additional water samples; and again in 2015 to recover the instrumentation and to collect another round of water samples and benthic images. All activities were conducted by SCUBA divers at five locations in the municipalities of Mabini and Tingloy, including Batong Buhay, Koala Reserve Area, Arthur's Reef, Twin Rocks, and Batalang Bato. At each of the five locations, a shallow and deep survey site was established for a total of 10 survey sites. |
Keywords
Theme Keywords
Thesaurus | Keyword |
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ISO 19115 Topic Category |
biota
|
ISO 19115 Topic Category |
oceans
|
UNCONTROLLED | |
CoRIS Discovery Thesaurus | Numeric Data Sets > Biology |
CoRIS Theme Thesaurus | EARTH SCIENCE > Biosphere > Aquatic Habitat > Benthic Habitat |
CoRIS Theme Thesaurus | EARTH SCIENCE > Biosphere > Aquatic Habitat > Reef Habitat |
CoRIS Theme Thesaurus | EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment |
CoRIS Theme Thesaurus | EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Autonomous Reef Monitoring Structure (ARMS) |
CoRIS Theme Thesaurus | EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Baseline studies |
CoRIS Theme Thesaurus | EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > In Situ Biological |
CoRIS Theme Thesaurus | EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs |
CoRIS Theme Thesaurus | EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs > Coral Reef Ecology |
CoRIS Theme Thesaurus | EARTH SCIENCE > Oceans > Marine Biology > Brachyura |
CoRIS Theme Thesaurus | EARTH SCIENCE > Oceans > Marine Biology > Cryptobiota |
CoRIS Theme Thesaurus | EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates |
CoRIS Theme Thesaurus | EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates > Biodiversity |
CoRIS Theme Thesaurus | EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates > Census |
CoRIS Theme Thesaurus | EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates > Macroinvertebrates |
CRCP Project | 483 |
CRCP Project | Climate, Biodiversity and Fisheries in the Coral Triangle: Embracing the E in Ecosystem Approaches to Fisheries Management |
NODC DATA TYPES THESAURUS | INVERTEBRATE SPECIES |
NODC DATA TYPES THESAURUS | SPECIES IDENTIFICATION |
NODC DATA TYPES THESAURUS | SPECIES IDENTIFICATION - COUNT |
NODC OBSERVATION TYPES THESAURUS | survey - biological |
NODC OBSERVATION TYPES THESAURUS | visual estimate |
NODC OBSERVATION TYPES THESAURUS | visual observation |
NODC PROJECT NAMES THESAURUS | Coral Reef Conservation Program |
NODC PROJECT NAMES THESAURUS | CORAL REEF STUDIES |
NODC PROJECT NAMES THESAURUS | Coral Triangle Initiative |
NODC SUBMITTING INSTITUTION NAMES THESAURUS | US DOC; NOAA; NMFS; Pacific Islands Fisheries Science Center; Ecosystem Sciences Division; Coral Reef Ecosystem Program |
None | Coral Reef Ecosystem Division |
None | Coral Reef Ecosystem Program |
None | CRED |
None | CREP |
None | Pacific Islands Fisheries Science Center |
None | PIFSC |
None | United States Agency for International Development |
None | USAID |
Spatial Keywords
Thesaurus | Keyword |
---|---|
UNCONTROLLED | |
CoRIS Place Thesaurus | COUNTRY/TERRITORY > Philippines > Batangas > Arthur's Rock (13N120E0002) |
CoRIS Place Thesaurus | COUNTRY/TERRITORY > Philippines > Batangas > Mabini (13N120E0032) |
CoRIS Place Thesaurus | COUNTRY/TERRITORY > Philippines > Batangas > Tingloy (13N120E0009) |
CoRIS Place Thesaurus | COUNTRY/TERRITORY > Philippines > Batangas > Twin Rocks Sanctuary (13N120E0005) |
CoRIS Place Thesaurus | OCEAN BASIN > Pacific Ocean > South China Sea > Balayan Bay > Arthur's Rock (13N120E0002) |
CoRIS Place Thesaurus | OCEAN BASIN > Pacific Ocean > South China Sea > Balayan Bay > Mabini (13N120E0032) |
CoRIS Place Thesaurus | OCEAN BASIN > Pacific Ocean > South China Sea > Balayan Bay > Twin Rocks Sanctuary (13N120E0005) |
CoRIS Place Thesaurus | OCEAN BASIN > Pacific Ocean > South China Sea > Maricaban Island > Tingloy (13N120E0009) |
NODC SEA AREA NAMES THESAURUS | NW Pacific (limit-180) |
None | Arthur's Reef |
None | Batalang Bato |
None | Batong Buhay |
None | Koala Reserve Area |
None | Philippines |
None | Twin Rocks |
None | Verde Island Passage |
Instrument Keywords
Thesaurus | Keyword |
---|---|
UNCONTROLLED | |
None | ARMS |
None | Autonomous Reef Monitoring Structures |
Physical Location
Organization: | Pacific Islands Fisheries Science Center |
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City: | Honolulu |
State/Province: | HI |
Country: | USA |
Data Set Information
Data Set Scope Code: | Data Set |
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Data Set Type: | CSV Files |
Maintenance Frequency: | None Planned |
Data Presentation Form: | Table (digital) |
Entity Attribute Overview: |
Specimen retrieval data includes metadata for each ARMS unit (where, deployment / retrieval date) and individual specimens identified to the lowest taxonomic level possible along with a count. Each column is described in the entities and attributes section. |
Entity Attribute Detail URL: | https://www.fisheries.noaa.gov/inport/item/45936 |
Distribution Liability: |
While every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty. |
Data Set Credit: | PIFSC Coral Reef Ecosystem Program and funded by the NOAA Coral Reef Conservation Program and the United States Agency for International Development |
Support Roles
Data Set Credit
Date Effective From: | 2012 |
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Date Effective To: | |
Contact (Organization): | NOAA Coral Reef Conservation Program (CRCP) |
Address: |
1305 East West Highway 10th Floor Silver Spring, MD 20910-3281 |
Phone: | (301) 713-3155 |
URL: | https://coralreef.noaa.gov |
Data Steward
Date Effective From: | 2012 |
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Date Effective To: | |
Contact (Person): | Timmers, Molly A |
Address: |
1845 Wasp Blvd. Honolulu, HI 96818 USA |
Email Address: | molly.timmers@noaa.gov |
Phone: | (808)725-5449 |
Contact Instructions: |
Email preferred |
Distributor
Date Effective From: | 2017 |
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Date Effective To: | |
Contact (Organization): | NOAA National Centers for Environmental Information (NCEI) |
Email Address: | ncei.info@noaa.gov |
URL: | NCEI Contact Information |
Metadata Contact
Date Effective From: | 2021 |
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Date Effective To: | |
Contact (Person): | Olenski, Brooke |
Email Address: | brooke.olenski@noaa.gov |
Originator
Date Effective From: | 2012 |
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Date Effective To: | |
Contact (Organization): | Pacific Islands Fisheries Science Center (PIFSC) |
Address: |
1845 Wasp Blvd. Honolulu, HI 96818 USA |
Email Address: | pifsc.info@noaa.gov |
Phone: | 808-725-5360 |
URL: | https://www.pifsc.noaa.gov |
Business Hours: | 8:00 a.m. - 4:30 p.m. |
Point of Contact
Date Effective From: | 2015 |
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Date Effective To: | |
Contact (Person): | Oliver, Thomas |
Address: |
1845 Wasp Blvd. Honolulu, HI 96818 USA |
Email Address: | thomas.oliver@noaa.gov |
Phone: | (808)725-5444 |
Contact Instructions: |
Email preferred |
Extents
Currentness Reference: | Ground Condition |
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Extent Group 1
Extent Description: |
Mabini and Tingloy in Batangas, Philippines |
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Extent Group 1 / Geographic Area 1
W° Bound: | 120.871943 | |
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E° Bound: | 120.895127 | |
N° Bound: | 13.728054 | |
S° Bound: | 13.658594 | |
Description |
Five survey locations in the municipalities of Mabini and Tingloy in Batangas, Philippines (near the Verde Island Passage), including Batong Buhay, Koala Reserve Area, Arthur's Reef, Twin Rocks, and Batalang Bato. |
Extent Group 1 / Time Frame 1
Time Frame Type: | Range |
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Start: | 2012-03-12 |
End: | 2015-05-31 |
Alternate Start As Of Info: | SBP1201 - deployed in 2012 |
Alternate End As Of Info: | MP1507 - recovered in 2015 |
Description: |
Time frame of the dataset from when the units were first deployed in 2012 to when they were last recovered in 2015. |
Access Information
Security Class: | Unclassified |
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Security Classification System: |
Not applicable |
Security Handling Description: |
Not applicable |
Data Access Policy: |
NOAA Coral Reef Ecosystem Program (CREP) Data Sharing Recommendations, version 9.0 updated August 12, 2015: CREP welcomes the opportunity to collaborate on research issues contributing to the scientific basis for better management of marine ecosystems. CREP has a very diverse set of field activities that generates large volumes of data using an array of data collection protocols. The following recommendations are for your consideration as you use this data: 1) Data analyses should take all field exigencies into account. The most effective way to do this would be active collaboration with CREP principal investigators. 2) In all presentations, product releases, or publications using data generated by CREP, proper acknowledgement of both CREP and the individuals responsible for data collection is expected. Citing the DOI (if available) is preferred, a non-DOI example is listed below. 3) If you collect or generate data for the same study areas, CREP requests that you share relevant information on complimentary data collections. 4) Those receiving data are strongly urged to inform the CREP Data Management Team of any errors and discrepancies that are discovered during the course of using these data. They are further urged to bring to the attention of the Team all problems and difficulties encountered in using these data. This information is necessary in order to improve the collections and to facilitate more efficient and economical data processing and retrieval. The users are asked to supply copies of any missing data that may be located, and to provide information as to significant subsets and special aggregations of data that are developed in using the material provided. Example citation: "This publication makes use of data products provided by the Coral Reef Ecosystem Program (CREP), Pacific Islands Fisheries Science Center (PIFSC), National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA), with funding support from the NOAA Coral Reef Conservation Program (CRCP) and the United States Agency for International Development. The analysis and interpretations presented here are solely that of the current authors.” |
Data Access Procedure: |
Data can be accessed online via the NOAA National Centers for Environmental Information (NCEI) Ocean Archive. |
Data Access Constraints: |
None |
Data Use Constraints: |
Please cite NOAA Coral Reef Ecosystem Program (CREP) when using the data. Suggested citation: Coral Reef Ecosystem Program; Pacific Islands Fisheries Science Center (2017). Assessing cryptic reef diversity of colonizing marine invertebrates using Autonomous Reef Monitoring Structures (ARMS) deployed at coral reef sites in Batangas, Philippines from 2012 to 2015. NOAA's National Center for Environmental Information, https://www.fisheries.noaa.gov/inport/item/45818. |
Metadata Access Constraints: |
None |
Metadata Use Constraints: |
None |
Distribution Information
Distribution 1
Download URL: | https://accession.nodc.noaa.gov/0162829 |
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Distributor: | NOAA National Centers for Environmental Information (NCEI) (2017 - Present) |
Description: |
Reference table for species recovered in the Philippines in 2015. |
File Date/Time: | 2021-03-03 00:00:00 |
File Type (Deprecated): | csv (comma-separated values) |
Distribution Format: | CSV - Comma Separated Values (Text) |
Distribution 2
Start Date: | 2012-03-12 |
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End Date: | 2015-05-31 |
Download URL: | https://accession.nodc.noaa.gov/0162829 |
Distributor: | NOAA National Centers for Environmental Information (NCEI) (2017 - Present) |
File Name: | ARMS_Specimens_Philippines_2015.csv |
Description: |
ARMS specimens recovered by the NOAA Coral Reef Ecosystem Program (CREP) from the Philippines in 2015. |
File Date/Time: | 2017-05-03 00:00:00 |
File Type (Deprecated): | csv (comma-separated values) |
Distribution Format: | CSV - Comma Separated Values (Text) |
URLs
URL 1
URL: | https://origin-apps-pifsc.fisheries.noaa.gov/cred/index.php |
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Name: | NOAA CRED |
URL Type: |
Online Resource
|
File Resource Format: | PHP |
Description: |
Information on NOAA CRED's official website |
URL 2
URL: | https://origin-apps-pifsc.fisheries.noaa.gov/cred/images/arms_install_sm.jpg |
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Name: | A NOAA diver installing an ARMS unit. |
URL Type: |
Browse Graphic
|
File Resource Format: | JPEG |
URL 3
URL: | https://origin-apps-pifsc.fisheries.noaa.gov/cred/images/arms_deployed4_sm.jpg |
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Name: | ARMS recruitment after 2 years |
URL Type: |
Browse Graphic
|
File Resource Format: | JPEG |
URL 4
URL: | https://origin-apps-pifsc.fisheries.noaa.gov/cred/coral_triangle_initiative.php |
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Name: | Coral Triangle Initiative |
URL Type: |
Online Resource
|
File Resource Format: | PHP |
Description: |
Information about the NOAA Coral Reef Ecosystem Program's activities in the Coral Triangle. |
URL 5
URL: | https://pifscblog.wordpress.com/2013/03/06/verde-island-passage/ |
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Name: | Blog Post |
URL Type: |
Online Resource
|
File Resource Format: | HTML |
Description: |
NOAA PIFSC Coral Reef Ecosystem Program blog post from the 2013 mission to the Philippines. |
URL 6
URL: | https://origin-apps-pifsc.fisheries.noaa.gov/cred/survey_methods/arms/overview.php |
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Name: | ARMS Overview |
URL Type: |
Online Resource
|
Description: |
NOAA's Official page for ARMS |
Technical Environment
Description: |
Microsoft Access |
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Data Quality
Accuracy: |
All species identifications are made visually by a trained analyst and subsequently reviewed by a taxonomic expert or through molecular processing for accuracy. |
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Completeness Report: |
All ARMS units that are recovered are disassembled, photographed, and sorted by size. Taxonomic classification occurs for materials greater than 2 mm. The research and development of the genetic analysis of ARMS samples is being done in collaboration with partners and data may exist for a subset of locations. ARMS that have been deployed may not have been recovered due to logistical constraints of the following mission or could not be found when divers returned to the site. |
Conceptual Consistency: |
ARMS sample biodiversity over precisely the same surface area in the exact same manner. Thus, the use of ARMS is a systematic, consistent, and comparable method for monitoring the cryptobiota community overtime. Three units are deployed at each site to allow for replicate measurements. Divers typically record ARMS metadata into the master Microsoft Access database within a few days of the field operations and/or ARMS processing. QA/QC procedures are typically completed during the field mission. |
Quality Control Procedures Employed: |
The data entered in the MS Access database is quality controlled following data entry. |
Data Management
Have Resources for Management of these Data Been Identified?: | Yes |
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Approximate Percentage of Budget for these Data Devoted to Data Management: | Unknown |
Do these Data Comply with the Data Access Directive?: | Yes |
Is Access to the Data Limited Based on an Approved Waiver?: | No |
Approximate Delay Between Data Collection and Dissemination: | Unknown |
Actual or Planned Long-Term Data Archive Location: | NCEI-MD |
Approximate Delay Between Data Collection and Archiving: | Unknown |
How Will the Data Be Protected from Accidental or Malicious Modification or Deletion Prior to Receipt by the Archive?: |
NOAA IRC and NOAA Fisheries ITS resources and assets. The MS Access database is stored on the PIFSC network and regularly backed up by ITS. |
Lineage
Lineage Statement: |
Autonomous Reef Monitoring Structures (ARMS) are assembled, deployed on the benthos for 1-3 years during which time they are colonized with marine organisms, recovered and processed as described below. The > 2-mm organisms are identified and counted, and the data is recorded in an MS Access database. The smaller fractions are sent for genomic analysis, although those data are not included with this dataset. Once this data is published it will be accessible with it’s respective metadata record in the “Related Items” section below. |
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Sources
Autonomous Reef Monitoring Structures (ARMS) Overview
Contact Role Type: | Originator |
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Contact Type: | Person |
Contact Name: | Molly Timmers |
Citation URL: | https://origin-apps-pifsc.fisheries.noaa.gov/cred/survey_methods.php#arms |
Citation URL Name: | Pacific Islands Fisheries Science Center website |
Source Contribution: |
Protocol |
PIFSC Coral Reef Ecosystem Program (CREP) Survey Methods - Autonomous Reef Monitoring Structures (ARMS)
Contact Type: | Organization |
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Contact Name: | Pacific Islands Fisheries Science Center, PIFSC |
Citation URL: | https://www.pifsc.noaa.gov/cred/survey_methods.php#arms |
Process Steps
Process Step 1
Description: |
ARMS Deployment - The ARMS platform consists of 23 cm x 23 cm grey, type 1 PVC plates stacked in alternating series of 4 open and 4 obstructed layers and attached to a base plate of 35 cm x 45 cm which is affixed to the reef. They are affixed to the sea floor with either four stainless steel stakes or weights and zip ties and are typically deployed on mid-depth (10-15 meters) forereef habitats in replicate sets of three. Each ARMS unit is typically separated by 2-5 meters. A GPS waypoint of the site is obtained by swimming over the site to get a point directly above the ARMS unit. The ARMS site and ARMS units are photo documented; pictures of the surrounding habitat as well as the deployed ARMS are captured. Close-up images of the dominant benthic cover around the ARMS units are captured. ARMS remain on the bottom for a set period of time during which they become colonized with marine organisms. The soak time varies by unit model. Please see the data to determine how long a particular unit was underwater. |
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Process Contact: | Reardon, Kerry G |
Phone (Voice): | 808-725-5465 |
Email Address: | kerry.reardon@noaa.gov |
Source: | PIFSC Coral Reef Ecosystem Program (CREP) Survey Methods - Autonomous Reef Monitoring Structures (ARMS) |
Process Step 2
Description: |
ARMS Recovery and Processing - Photo documentation occurs of the ARMS and recovery site before the ARMS units are removed off of the benthos. The ARMS unit is detached from the substrate, encapsulated, brought to the surface, and disassembled and processed onboard the research ship or in the field for shore-based/fly-in operations. Disassembled plates are photographed to document recruited sessile organisms. The plates are rinsed lightly in a container to remove sand particles thereby providing a cleaner surface for imaging the sessile organisms on the plates. Each plate is placed in a shallow tray containing seawater to be photographed. An initial photo of the plate is obtained along with a close up image of each quarter of the plate, the center, and of anything of interest. Photos are obtained of the top and bottom of each plate in the unit. Images are used for analyses of sessile recruitment and composition. When all of the plate layers in the ARMS unit have been photographed and set aside (in seawater), the seawater from the disassembly tub, photo tray, and rinse bucket is sieved through adjoining 2 mm and 500 um sieve pans and an attachable 100 um mesh hand net. Material collected in the 500 um sieve and 100 um net are bulk preserved into two separate jars. Jars are filled with EtOH and labeled accordingly. The preserved 500 and 100 um sample fractions undergo mass sequencing techniques. The > 2 mm size fraction can either be bulked preserved, like the 500 and 100 um fractions, with the understanding that they will be sorted at a later date or can be sorted at the time of processing into morphospecies. Sorting the > 2 mm size fraction is more efficient immediately after processing because the organisms are alive, intact, and colorful. Ethanol, as a preservative, fades away specimen coloration, can separate annelid segments and can detach crustacean limbs when bulk preserved. Immediate processing of the > 2 mm size fraction also provides you with the opportunity to photograph the specimens for vouchering. When photographing specimens, the first image has the unique specimen label in the image. Subsequent images may be taken without the label for finer details. When images and identifications are complete, the specimen(s) are preserved in ethanol. All plates from an individual ARMS unit are scrapped en masse. Once all plates have been scraped, all the scrapings are transferred into a blender (Brevill; BBL600XL). The scrapings are blended for 45-60 seconds on maximum power until sample is homogenized. The sample is then transferred from the blender to a 40 um net. The sample in the net is rinsed with filtered (< 40 um) seawater until all discharge from net is clear (takes ~2 gal). Four ~10 ml samples are preserved in 50 ml falcon tubes with DMSO or 95% EtOH, secure lid and shake. The remaining sample is stored in a sterile whirlpak at -20C. Between the processing of each ARMS unit the blender is rinsed in fresh water to remove any remaining homogenate. The blender is then placed in a 10% bleach solution for 15 minutes. Finally all parts thoroughly rinsed with DI water if available or fresh water. All recovered ARMS units are processed to the above step. When possible, ARMS samples are analyzed molecularly and taxonomically. The data resulting from genetic analysis of ARMS samples using 454 Illumina mass sequencing techniques through partnerships with the Smithsonian, San Diego State University, Moss Landing Marine Laboratories, and the Hawaii Institute of Marine Biology, can be found through it’s respective metadata record in the “Related Items” section below. |
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Process Contact: | Timmers, Molly A |
Phone (Voice): | (808)725-5449 |
Email Address: | molly.timmers@noaa.gov |
Source: | PIFSC Coral Reef Ecosystem Program (CREP) Survey Methods - Autonomous Reef Monitoring Structures (ARMS) |
Catalog Details
Catalog Item ID: | 45818 |
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GUID: | gov.noaa.nmfs.inport:45818 |
Metadata Record Created By: | Annette M DesRochers |
Metadata Record Created: | 2017-04-21 23:43+0000 |
Metadata Record Last Modified By: | SysAdmin InPortAdmin |
Metadata Record Last Modified: | 2024-10-03 18:16+0000 |
Metadata Record Published: | 2017-06-09 |
Owner Org: | PIFSC |
Metadata Publication Status: | Published Externally |
Do Not Publish?: | N |
Metadata Last Review Date: | 2017-06-09 |
Metadata Review Frequency: | 1 Year |
Metadata Next Review Date: | 2018-06-09 |