Tbl: RNADNA QA
Entity (ENT) | Alaska Fisheries Science Center (AFSC)GUID: gov.noaa.nmfs.inport:23799 | Updated: August 9, 2022 | Published / External
Summary
Item Identification
Entity Info
Data Attributes
Catalog Details
Data Attributes
Attribute Summary
|
|
Name | Type | Description |
|---|---|---|---|---|
|
100
|
Batch | Unknown | Either string name or when multiple strings were analyzed the same day the date the analysis was done, the initials of the analyst, and the plate number (mmddyyRB1). | |
|
100
|
slope ratio | Unknown | Observed DNA:RNA standard slope ratio. Target = 3.66; Agreement of DNA and RNA standards between runs | |
|
100
|
PolRef07 | Unknown | value of RNA/DNA; Accuracy comparing current control reference values to avg 2.83 of previous runs | |
|
100
|
Mblk (total) | Unknown | Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 | |
|
100
|
Mblk (RNase addn) | Unknown | Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 | |
|
100
|
Mblk (DNase addn) | Unknown | Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 |
Attribute Details
Batch
CC ID: 191257
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
Either string name or when multiple strings were analyzed the same day the date the analysis was done, the initials of the analyst, and the plate number (mmddyyRB1). |
| Allowed Values: | No domain defined. |
slope ratio
CC ID: 191258
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
Observed DNA:RNA standard slope ratio. Target = 3.66; Agreement of DNA and RNA standards between runs |
| Allowed Values: | No domain defined. |
PolRef07
CC ID: 191259
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
value of RNA/DNA; Accuracy comparing current control reference values to avg 2.83 of previous runs |
| Allowed Values: | No domain defined. |
Mblk (total)
CC ID: 191260
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 |
| Allowed Values: | No domain defined. |
Mblk (RNase addn)
CC ID: 191261
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 |
| Allowed Values: | No domain defined. |
Mblk (DNase addn)
CC ID: 191262
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 |
| Allowed Values: | No domain defined. |