Tbl: RNADNA QA
Entity (ENT) | Alaska Fisheries Science Center (AFSC)GUID: gov.noaa.nmfs.inport:23799 | Updated: August 9, 2022 | Published / External
Entity Information
| Description: |
-no description- |
|---|
Data Attributes
Attribute Summary
|
|
Name | Type | Description |
|---|---|---|---|---|
|
100
|
Batch | Unknown | Either string name or when multiple strings were analyzed the same day the date the analysis was done, the initials of the analyst, and the plate number (mmddyyRB1). | |
|
100
|
slope ratio | Unknown | Observed DNA:RNA standard slope ratio. Target = 3.66; Agreement of DNA and RNA standards between runs | |
|
100
|
PolRef07 | Unknown | value of RNA/DNA; Accuracy comparing current control reference values to avg 2.83 of previous runs | |
|
100
|
Mblk (total) | Unknown | Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 | |
|
100
|
Mblk (RNase addn) | Unknown | Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 | |
|
100
|
Mblk (DNase addn) | Unknown | Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 |
Attribute Details
Batch
CC ID: 191257
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
Either string name or when multiple strings were analyzed the same day the date the analysis was done, the initials of the analyst, and the plate number (mmddyyRB1). |
| Allowed Values: | No domain defined. |
slope ratio
CC ID: 191258
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
Observed DNA:RNA standard slope ratio. Target = 3.66; Agreement of DNA and RNA standards between runs |
| Allowed Values: | No domain defined. |
PolRef07
CC ID: 191259
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
value of RNA/DNA; Accuracy comparing current control reference values to avg 2.83 of previous runs |
| Allowed Values: | No domain defined. |
Mblk (total)
CC ID: 191260
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 |
| Allowed Values: | No domain defined. |
Mblk (RNase addn)
CC ID: 191261
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 |
| Allowed Values: | No domain defined. |
Mblk (DNase addn)
CC ID: 191262
| Data Storage Type: | Unknown |
|---|---|
| Required: | No |
| Primary Key: | No |
| Status: | Active |
| Description: |
Interference (average fluorescence readings from wells with 5 microg/mL ethidium bromide, 0.1 % STEB): NTE 2000 |
| Allowed Values: | No domain defined. |
Catalog Details
| Catalog Item ID: | 23799 |
|---|---|
| GUID: | gov.noaa.nmfs.inport:23799 |
| Metadata Record Created By: | Emily A Fergusson |
| Metadata Record Created: | 2015-03-12 21:13+0000 |
| Metadata Record Last Modified By: | SysAdmin InPortAdmin |
| Metadata Record Last Modified: | 2022-08-09 17:11+0000 |
| Metadata Record Published: | 2016-05-18 |
| Owner Org: | AFSC |
| Metadata Publication Status: | Published Externally |
| Do Not Publish?: | N |
| Metadata Last Review Date: | 2016-05-18 |
| Metadata Review Frequency: | 1 Year |
| Metadata Next Review Date: | 2017-05-18 |