Ocean acidification impacts on black sea bass and scup embryos, responses of finfish in laboratory experiments

Black sea bass (Centropristis striata) and scup (Stenotomus chrysops) compose important recreational and commercial fisheries along the United States Atlantic coast. Black sea bass is a temperate species, associated with reef habitat. Wild stocks and landings have been decreasong in recent decades. The demand for black sea bass exceeds supply, and the high market value has prompted research to evaluate their potential for commercial aquaculture. Recent studies conducted at the National Marine Fisheries Service, Milford, CT laboratory examined growth rates of juvenile scup fed commercial diets. This and other on-going studies at Milford have shown scup to acclimate quickly to tank conditions in the laboratory, and to exhibit rapid growth rates. These studies indicate the possibility that scup have potential as a candidiate species for commercial aquaculture. Studies with both fish species suggest they are interesting species for studies of the effects of ocean acidification because of their economic importance as fisheries species. These studies focused on laboratory-based experiments to measure the biological effects of elevated levels of CO2 on embryos of these important marine finfish. Adult black sea bass were naturally conditioned and spawned in the laboratory by photo-thermal manipulation. Adult scup were strip-spawned at sea and their eggs were fertilized at sea. The fertilized eggs of both species of fish were exposed to two treatment levels of pCO2 and one control level, with three replicates per treatment and the controls. Measurements of biological effects included percent hatch, viable embryos, abnormal embryos, and dead embryos. Measurements of dissolved oxygen concentration, percent oxygen saturation, temperature, salinity and pH were taken daily in each treatment container and the controls. Samples of seawater were taken at the time of intial experimental setup and at the time of hatching from each container for analyses of dissolved inorganic carbon (DIC), and analyses of pH by spectrometry.

To determine impacts of ocean acidification on embryos of two species of marine fish.

There will be a digital object identifier and a URL that can be accessed.

NEFSC Data Access Procedure:

1. Formal request in writing usually to the data owner/contact or Center Director;

2. Requester is contacted by data owner to review and verify the request content and details for data delivery options.

3. If data is confidential then owner will determine if the data may be released to the requester;

4. If data can be released, the data is downloaded and packaged for delivery electronically; or the requester may be directed to where the data is available online.

Data can not be accessed until publication of manuscript in 2017.

NEFSC Data Access Constraint:

If data is stored on an internal NEFSC network, the requester may not have direct access to query the data unless they are also authorized for network access.

MS Excel spreadsheet

The accuracy is represented by two experienced scientists conducting visual microscopic observations and checking each others observations for accuracy during the experiment and during data entry.

Data validation during data collection and entry: Every sample was analyzed by two scientists for comparison of reliable, consistent results. Portable pH meter used for comparison with UV spec. data.

Archival of source data preserving unaltered collected data.

none