Short Citation:
Alaska Fisheries Science Center, 2021: AFSC/RACE/SAP/Pathobiology: 2014 Bitter crab disease prevalence in immature Chionoecetes spp. at 6 index sites in eastern Bering Sea, https://www.fisheries.noaa.gov/inport/item/26477.

Item Identification

Title: AFSC/RACE/SAP/Pathobiology: 2014 Bitter crab disease prevalence in immature Chionoecetes spp. at 6 index sites in eastern Bering Sea
Short Name: AFSC/RACE/SAP/Pathobiology: 2014 Bitter crab disease prevalence in immature Chionoecetes spp. at 6 index sites in eastern Bering Sea
Status: In Work
Abstract:

This dataset contains crab data from a field survey of Chionoecetes bairdi and C. opilio collected at six designated index sites in the Bering Sea during the 2014 NOAA/NMFS/AFSC/RACE crab-groundfish bottom trawl survey of the eastern Bering Sea. Each of the six index sites were made up of approximately 10 survey stations and chosen based on historical incidences of the parasite Hematodinium sp., the causative agent of Bitter Crab Syndrome (BCS). Crab collected at each survey station were preserved and tested in a laboratory with a DNA test for the presence of Hematodinium sp. parasite DNA to evaluate any changes in intensity of disease within the index sites that may provide information as to how the disease is moving or changing within EBS crab populations. The data includes index site, species, crab morphometrics, Hematodinium sp. parasite presence/absence based on PCR assay results.

Purpose:

To track the relative prevalence of Hematodinium within Chionoecetes spp. and be able to detect major changes annually.

Notes:

Loaded by FGDC Metadata Uploader, batch 9591, 12-16-2016 10:52

The following FGDC sections are not currently supported in InPort, but were preserved and will be included in the FGDC export:

- Taxonomy (FGDC:taxonomy)

Keywords

Theme Keywords

Thesaurus Keyword
UNCONTROLLED
ISO19115 Biota
ISO19115 Oceans
None bottom trawl
None disease

Temporal Keywords

Thesaurus Keyword
UNCONTROLLED
None summer

Spatial Keywords

Thesaurus Keyword
UNCONTROLLED
None Alaska
None Bering Sea
None Bristol Bay
None Pribilof Islands

Physical Location

Organization: Alaska Fisheries Science Center
City: Seattle
State/Province: WA
Country: USA

Data Set Information

Data Set Scope Code: Data Set
Maintenance Frequency: As Needed
Data Presentation Form: Table (digital)
Distribution Liability:

The user is responsible for the results of any application of this data for other than its intended purpose.

NOAA denies liability if the data are misused.

Support Roles

Data Steward

CC ID: 232572
Date Effective From: 2015
Date Effective To:
Contact (Person): Jensen, Pam
Email Address: pam.jensen@noaa.gov
Contact Instructions:

email

Distributor

CC ID: 365319
Date Effective From: 2016-12-16
Date Effective To:
Contact (Person): Foy, Robert
Email Address: robert.foy@noaa.gov

Metadata Contact

CC ID: 365317
Date Effective From: 2016-12-16
Date Effective To:
Contact (Person): MC, Metadata Coordinators
Email Address: AFSC.metadata@noaa.gov

Originator

CC ID: 365320
Date Effective From: 2016-12-16
Date Effective To:
Contact (Organization): Alaska Fisheries Science Center (AFSC)
Address: 7600 Sand Point Way N.E., Building 4
Seattle, WA 98115
USA
URL: http://www.afsc.noaa.gov
Business Hours: 0700-1700 Pacific Time

Point of Contact

CC ID: 365318
Date Effective From: 2016-12-16
Date Effective To:
Contact (Person): Jensen, Pam
Email Address: pam.jensen@noaa.gov

Extents

Currentness Reference: Ground Condition

Extent Group 1

Extent Group 1 / Geographic Area 1

CC ID: 365316
W° Bound: -176.5
E° Bound: -158
N° Bound: 60.5
S° Bound: 54
Description

Eastern Bering Sea, Alaska

Extent Group 1 / Time Frame 1

CC ID: 365315
Time Frame Type: Discrete
Start: 2014

Access Information

Security Class: Unclassified
Data Access Procedure:

unknown

Data Access Constraints:

There are no legal restrictions on access to the data. They reside in public domain and can be freely distributed.

Data Use Constraints:

User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement

of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested.

Distribution Information

Technical Environment

Description:

Excel

Data Quality

Accuracy:

Data is submitted through a thorough QA/QC process.

Completeness Report:

Data set includes data collected from standard and non-standard hauls.

Conceptual Consistency:

Data is checked for values outside range.

Quality Control Procedures Employed:

unknown

Data Management

Have Resources for Management of these Data Been Identified?: No
Approximate Percentage of Budget for these Data Devoted to Data Management: Unknown
Do these Data Comply with the Data Access Directive?: No
Is Access to the Data Limited Based on an Approved Waiver?: No
If Distributor (Data Hosting Service) is Needed, Please Indicate: yes
Approximate Delay Between Data Collection and Dissemination: unknown
If Delay is Longer than Latency of Automated Processing, Indicate Under What Authority Data Access is Delayed:

No delay

Actual or Planned Long-Term Data Archive Location: NCEI-MD
Approximate Delay Between Data Collection and Archiving: Unknown
How Will the Data Be Protected from Accidental or Malicious Modification or Deletion Prior to Receipt by the Archive?:

IT Security and Contingency Plan for the system establishes procedures and applies to the functions, operations, and resources necessary to recover and restore data as hosted in the Western Regional Support Center in Seattle, Washington, following a disruption.

Lineage

Process Steps

Process Step 1

CC ID: 365342
Description:

This dataset contains randomly chosen samples of crab collected from the annual NOAA/NMFS/AFSC/RACE crab-groundfish bottom trawl survey of the eastern Bering Sea continental shelf. The standard survey area, surveyed each year since 1975, encompasses a major portion of the eastern Bering Sea shelf between the 20 meter and 200 meter isobaths and from the Alaska Peninsula to the north of St. Matthew Island. The study area is divided into a grid with cell sizes of 20 x 20 nautical miles (37 x 37 kilometers). Sampling takes place within each 20 x 20 nautical mile grid cell. Each tow is one-half hour in duration, averaging 1.54 nautical miles (2.86 kilometers) - exact tow duration and distance fished for each haul can be found in RACEBASE.HAUL. 

Process Step 2

CC ID: 365343
Description:

Six specific index sites were chosen (three C. bairdi sites and three C. opilio sites) based on historical incidences of the parasite Hematodinium sp. the causative agent of Bitter Crab Syndrome (BCS). Index sites are located in Bristol Bay, Pribilof Islands, St. Matthew Island and the northern shelf edge (within stratum 61) and northern stations (within stratum 82) and each site included approximately 10 survey stations. At each station 20 immature crab were randomly selected, if less than 20 were available, additional crab were selected at another station within the index site. Whole crab or legs from crab were preserved by freezing and shipped frozen to Seattle (AFSC).

Process Step 3

CC ID: 365344
Description:

The sex of the crab is identified (1 = Male, 2 = Female, 3 = Unknown). Individual crab carapaces were measured (± 1 mm), excluding spines, and are reported as size. Null or blank fields in data represent no size information reported for crab specimen.

Shell condition class serves as a semi-quantitative index of molt status and time in shell post-molt. Carapace shell condition was assigned to one of six classes according to specific criteria (0 = premolt or molting, 1 = soft and pliable, 2 = new hardshell both firm and clean, 3 = oldshell slightly worn, 4 = oldshell worn, 5 = very oldshell, 9 = no shell condition information reported for crab specimen).

Process Step 4

CC ID: 365345
Description:

All female crab abdomens were evaluated to determine reproductive condition based on the color of the eggs (0 = no eggs, 2 =purple, 3 = brown, 4 = orange, 5 = purple-brown, 6 = pink, 9 = no egg color information reported or does not apply), the condition of the eggs (0 = no eggs, 1 = uneyed, 2 = eyed, 3 = dead, 4 = empty egg cases, 9 = no egg condition information reported or does not apply), and the size of the egg clutch (0 = immature, 1 = mature female no eggs, 2 = trace to 1/8, 3 = 1/4, 4 = 1/2, 5 = 3/4, 6 = full, 9 = no egg clutch information reported or does not apply).

Process Step 5

CC ID: 365514
Description:

Frozen tissue was removed from leg muscle and placed into 100% ethanol. DNA was extracted using modified Ivanova et al. (2006) protocol.

Extracted DNA was assayed with two primer sets, targeting either 18S or ITS1 rDNA, to determine presence or absence of Hematodinium spp. Refer to metadata Point of Contact for PCR conditions. PCR Result is identified (0 = negative for Hematodinium DNA, 1 = positive for Hematodinium DNA, 3 = undetermined, at the limit of detection).

Child Items

Rubric scores updated every 15m

Rubric Score Type Title
Entity (ENT) 2014 BCS Index

Catalog Details

Catalog Item ID: 26477
Metadata Record Created By: Nancy Roberson
Metadata Record Created: 2015-08-17 14:04+0000
Metadata Record Last Modified By: SysAdmin InPortAdmin
Metadata Record Last Modified: 2019-06-04 13:14+0000
Metadata Record Published: 2017-02-21
Owner Org: AFSC
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Next Review Date: 2018-02-22