AFSC/RACE/SAP/Foy: Effects of ocean acidification on embryo stages of Tanner crab: Kodiak Island, Alaska.

To study the effects of ocean acidification we examined the effects of ocean acidification on the embryo stages of the economically important southern Tanner crab, Chionoecetes bairdi.

Ovigerous females were reared in one of 3 treatments: control (ambient pH ~8.1), pH 7.8, and pH 7.5 for 2 years. Embryos in year 1 were from oocytes developed in the field whereas embryos in

year 2 were from oocytes developed under acidified conditions. Datasets associated with this study include Brooding Duration, Carbonate Chemistry, Embryo Morphology, and Embryo Stages. Embryo

stages assessed monthly included detailed developmental stages from the prefuniculus formation through the prehatching stage. Embryo stages were not different among pH treatments. Embryo

morphology included egg area, egg diameter, yolk area, yolk diameter, embryo area, eye area, and eye diameter. Each morphological characteristic varied according to pH treatment using a principal

component analysis.

The purpose of the embryo experiments was to provide embryo morphology and the stage data and information on progression through stages associated with the three treatments

throughout embryological development.

Loaded by FGDC Metadata Uploader, batch 7254, 10-08-2015 10:41

The following FGDC sections are not currently supported in InPort, but were preserved and will be included in the FGDC export:

- Taxonomy (FGDC:taxonomy)

unknown

none

Must read and fully comprehend the metadata prior to data use; acknowledgement of the Originator when using the data set as a source;

sharing of data products developed using the source data set with the Originator; data should not be used beyond the limits of the source scale;

the data set is NOT a survey document and should not be utilized as such

MS Excel 2012

The accuracy of the embryo stage data is 100% due to consistency during stage specific measurement from experienced scientists with replicate readings when appropriate.

In addition, the frequency of observations was high during the experiment to ensure that the temporal changes were captured. The accuracy of the embryo morphology data is high

(less than 1% difference from actual) due to consistency during morphological measurement from experienced scientists with replicate readings when appropriate. In addition, the

frequency of observations was high during the experiment to ensure that the temporal changes were captured.

Embryo stage and morphometric data are complete (i.e. not filtered).

Embryo stage and morphometric information were broadly compared to previous studies on Tanner crab and to similar measurements made on similar crab species. No quantitative assessments of consistency were made.

For example, for comparing we used the study described in Mathis, Jeremy T., Jessica N. Cross,

and Nicholas R. Bates. "Coupling primary production and terrestrial runoff to ocean acidification and carbonate

mineral suppression in the eastern Bering Sea." Journal of Geophysical Research: Oceans (19782012) 116.C2 (2011).

unknown

No delay

IT Security and Contingency Plan for the system establishes procedures and applies to the functions, operations, and resources necessary to recover and restore data as hosted in the Western Regional Support Center in Seattle, Washington, following a disruption.