Data Management Plan (Deprecated)

Brown shrimp, Farfantepenaeus aztecus, and white shrimp, Litopenaeus setiferus, were held were held under natural light conditions before experiments. Experiments were conducted in twelve rectangular tanks (58 cm x 149 cm) under fluorescent lighting (7-10 microEinsteins/sec/sq m). Shrimp were placed in randomly-assigned tanks the day before observations were initiated at 0730 h, the lights were turned on, and observations of thepercentage of shrimp burrowed ( 1/2 of their body beneath the substrate) were recorded hourly (starting at 0830 h) throughout the daylight hours. For both species, burrowing rates decreased significantly as the substrate became courser. Brown shrimp burrowing was marginally affected by salinity with the lowest burrowing rates at 5 ppt white shrimp burrowing was not significantly affected by salinity. Large brown shrimp burrowed more than small and medium sized shrimp, but size did not significantly affect burrowing of white shrimp. The presence of a fish predator in the tanks did not affect burrowing of either species, but hunger level significantly affected burrowing for both species.

Gulf Of Mexico

Process Steps:

• After observations of behavior in laboratory experiments, data were entered into an Excel spreadsheet. A text file was created to describe these data and associated variables. Entered data were checked against the laboratory record books to minimize entry errors. The electronic file was also sorted and examined in a variety of ways to look for outliers, missing data, and other potential errors. Verified data files were then saved electronically on the Galveston Laboratory server and backed-up as needed.

Log books were used to record shrimp behavior observations from all experiments. When possible, treatments were randomly applied to tanks; the exception was for substrate experiments where different substrates remained in the same tanks throughout the experimental series. Shrimp from holding tanks, however, were randomly placed in tanks during all experiments. Shrimp were moved from holding tanks to experimental tanks in the afternoon on the day before observations were initiated. At 0730 h the next day, the lights came on, and observations on behavior were recorded hourly (starting at 0830 h) throughout the daylight hours (generally 10 hourly observations during the day). Black plastic curtains surrounded each tank, and observations were made through small portals to avoid disturbance. During each hourly observation period, individual shrimp were categorized into one of eight behaviors.

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The data resides on a secure government network requiring multi-factor authentication for network access.