National Status and Trends: Mussel Watch Program - Resurrection Bay Database

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Sample collection and data acquisition are described here: Field Sampling Procedures: Three locations in Resurrection Bay were identified as traditional harvest areas. At each location, subsistence shellfish comprised of co-located cockles, blue mussels, and softshell clams were hand collected at low tide in edible sized proportions. The field team was trained to follow the standard quality control and quality assurance of the NSandT MWP protocol (Lauenstein and Cantillo, 1996). The shellfish were identified in the field based on local traditional knowledge using common (colloquial) names before a subsequent scientific identification (Foster, 1991). Identifying the subsistence shellfish with both local traditional and scientific names facilitates communication of the analytical results to resource managers and local communities. For each species, adults of roughly the same shell length were sampled to assure similar initial conditions among test organisms (Salazar et al., 1995). Sufficient numbers of shellfish were collected to ensure adequate sample size for chemical analysis and histopathology. Precautions were also taken to assure that the organisms are transported unstressed to the hatchery for the flow-through study. From the hatchery, test organisms were sent to TDI Brooks in Texas for chemical analysis and Rutgers University, Haskin Shellfish Research Laboratory for histopathology analysis. Following the Mussel Watch quality control protocol for sampling and sample handling, a sample consists of a composite of 30 organisms put into labeled double Ziploc bags. For each species, three composite samples were collected at a time, one for each analytical method (trace elements, organics and histopathology), and kept chilled in an ice chest before shipment. Flow through system: Alutiiq Pride Shellfish Hatchery To effectively determine the interspecies concentration factors (ICFs), test organisms - adult market size shellfish (Salazar et al., 1995) - were to be brought to steady state concentration in a semi-controlled flow-through system. The Alutiiq Pride Shellfish Hatchery located in Resurrection Bay was used for the flow-through study. The hatchery facility is equipped with a tested and operational flow-through circulation system, which is owned and operated by the Alaskan Native communities of the Chugach region. The test species were placed separately into labeled nylon mesh bags or lantern nets (Figure 1), and deployed into a large flow-through chamber with circulating ambient water from the Bay. Mesh bags will be created from the commercially available tubular oyster netting used in aquaculture (ASTM, 2001). For each species, roughly 100 organisms were placed in each bag and labeled. It has been demonstrated that even if restrained within expandable mesh material, shellfish usually reposition themselves in the manner that allow them to use their filtration mechanisms efficiently (ASTM, 2001). Therefore after acclimatization, the test organisms are expected to survive in the flow-through chamber. In this assessment study, the test organisms were exposed to and acquired their food from water pumped from Resurrection Bay. Bay water was pumped into a holding tank before being dispensed to the flow-through chamber. The flow-through rate of the water was adjusted to correspond with 100% water exchange daily. This setting provided adequate filtration rates, but also contributed to eliminating most of the wastes generated by the test organisms. In addition, the flow-through rate of 100% was critical to ensure that water in the chamber was not oxygen depleted. (continued...)

(continued from above) The following describes the steps in sample collection. Step 1: background concentration An initial set of tissue samples was selected for analysis at the end of the field work. Those samples provided baseline information on the tissue contaminant content and histopathology of the test organisms. While the background analysis on contaminant body burden was conducted on single composite sample per species, the histopathology assessment was conducted on duplicated composites of all 4 species. This background information was used to assess the water quality in the subsistence harvest areas. Further, the background information was used to compare chemical concentrations between the traditional harvest areas and those of the study site. Step 2: steady state assessment After a month of acclimatization in the flow-through chamber, blue mussels and sofshell clams from all three sites were sampled each month for a length of 5 months. This set of samples was collected to assess concentration change with time. Therefore, for cost saving purposes, the littleneck clams were used as archetype of the clams in this step. Based on published data on mussels and clams, the body burden study state equilibrium was expected to be reached after three months of exposure (Salazar et al., 1995). However, the 5 months time period was conceptualized to ascertain that contaminant body burdens actually reached the steady state. Step 3: final contaminant body burden At the end of the fifth month following the acclimatization period, all 4 test species from the three sites were sampled for the final chemical analysis. Data from this analysis was used for comparison and ICF determination. A total of 38 individual composite tissue samples were collected and analyzed in addition to water parameters such as temperature, pH, dissolved oxygen and salinity, which was measured daily in the inflow chamber throughout the experiment. Chemical analysis Chemical analyses followed standard procedures used in the NOAA NSandT program (Kimbrough and Lauenstein 2007). A broad suite of chemicals were analyzed at each station, including metals, butyltins, PAHs, persistent organic compounds (PCBs, chlorinated pesticides, and the emerging flame retardant chemicals - PBDEs). a. Metals Samples were stored frozen until processed in the laboratory. Later, the samples were prepared for atomic absorption analysis (including cold vapor for mercury) and inductively coupled plasma mass spectrometric (ICP-MS) analysis by freeze drying and wet digestion. Dried samples were homogenized, weighed and digested in a sequence of heating steps in Teflon bombs with HNO3, HF and, boric acid. For analysis of Hg, the samples were digested based on a modified version of EPA method 245.5, using a concentrated H2SO4 and HNO3 digestion, followed by addition of KMnO4, and K2S2O8. Recalibration standards were run every 12 samples, and matrix modifiers were used as necessary. Quality control samples were processed in a manner identical to actual samples. A method blank was run with every 20 samples, or with every sample batch. Matrix spike/matrix spike duplicate (MS/MSD) samples were run with every 20 samples, or with every sample batch, also dependent upon whichever occurred most frequently. The spiking level was ten times the MDL. (continued...)

(continued from above) Reference materials were extracted and analyzed with each sample batch, while the method detection limit determined following the procedures outlined in CFR 40, part 136 (EPA, 2005). b. Organics (PAHs, PCBs, chlorinated pesticides) An aliquot of approximately 1 g of sample was weighed and oven dried at 63 - 56 degrees C to constant weight to determine wet/dry weight. For analyses, an aliquot of homogenized sample is chemically dried with sodium sulfate. After samples are spiked with surrogates the samples are extracted in a Soxhlet apparatus with dichloromethane on a hot sand bath for 8 hr and the extracts filtered through a funnel containing glass wool and sodium sulfate. The sample extract is then concentrated and solvent changed to about 2 ml of hexane. Silica gel/alumina column chromatography is utilized to concentrate and purify the samples before analysis. Quality control samples (method blank, matrix spike, and standard reference materials) was processed with each batch of samples in a manner identical to the samples, including matrix spikes. Quantification of PAHs and their alkylated homologues was performed by gas chromatography mass spectrometry (GC/MS) in the selected ion monitoring (SIM) mode. Chlorinated hydrocarbons (chlorinated pesticides and PCBs, Tables 3 and 4) were quantitatively determined by capillary gas chromatography with an electron capture detector (ECD). The calibration solutions that were analyzed as part of the analytical GC/ECD run preceded by no more than six samples and no more than six samples were run between calibration mixtures. c. Organotins An aliquot of freeze dried tissue samples was weighed and appropriate amounts of surrogate standards were added to all samples, matrix spikes, and blanks. Samples were extracted three times by agitation with tropolone in dichloromethane. The sample extract was then concentrated in a hot water bath and the extract was centrifuged and further concentrated. The solvent was exchanged to hexane and concentrated to a final volume of about 10 - 20 ml at which point only hexane remained. Hexylmagnesium bromide (2 M; Grignard reagent) was added to the sample extract under nitrogen and heated to hexylate the sample. After separation from the organic phase, pentane:CH2Cl2 (3/1, v/v) was added to the aqueous phase and the sample shaken vigorously. The pentane:CH2Cl2 extraction was done twice. The hexylated extract was dried by addition of anhydrous Na2SO4 and then concentrated. The extract was purified using silica gel/alumina column chromatography and the eluent was collected and concentrated on a water bath. Quality control samples (method blank, matrix spike, and standard reference materials) was processed in a manner identical to actual samples. The method detection limit was determined following the procedures outlined in CFR 40, part 136 (1999). The quantitative method was based on high resolution, capillary gas chromatography using flame photometric detection (GC/FPD). The method allowed for determination of tetrabutyltin (4BT), tributyltin (TBT), dibutyltin (DBT) and monobutyltin (MBT) quantitatively. d. Polybrominated Diphenyl Ethers In addition to the contaminants listed above, the characterization of PBDEs, a class of persistent organic pollutants commonly used as flame retardants, was also quantified. Sample preparation and extraction are similar to those established for PCBs. Selected PBDE congeners were measured using GC/MS in selected ion monitoring mode (SIM) coupled with capillary column. The method is capable of detecting PBDEs in complex matrices. (continued...)

(continued from above) Sample extracts were injected into a temperature-programmed GC/MS, operated in splitless mode. The capillary column was a DB-XLB (30 m x 0.25 mm ID and 0.1 um film thickness). The mass spectrometer was capable of scanning from 53 to 500 AMU every second or less and used 70 electron volts energy in electron impact ionizing mode. Quality control samples (method blank, matrix spike, and standard reference materials) was processed with each batch of samples in a manner identical to the samples, including matrix spikes. PBDEs have been identified as emerging contaminants of concern for ecosystems throughout the US by USEPA. PBDE has been found in Canadian and Alaskan fish and wildlife and was assumed to be of long distance atmospheric source (Braune et al., 2005). However these contaminants have not been extensively characterized for the Gulf of Alaska ecosystem nor have they been considered under any of the restoration strategies. The partnership with the NOAA NSandT is critical to these efforts because the program has developed appropriate techniques to evaluate PBDEs in tissue. e. Histopathology analysis In addition to the emphasis on the persistent contaminants listed above, the characterization of the histopathology of the targeted subsistence shellfish was also proposed. The histological analysis was performed at Rutgers University by the Haskin Shellfish Research Laboratory. It is a set of quantitative and semiquantitative analyses that determine the reproductive stage of, and occurrence of pathologies in shellfish. A detailed account of the protocol is described in the NOAA's NOS/NCCOS technical memorandum number 27 by Kim et al. (2006). From each sample composites, 5 individual organisms were randomly selected and prepared for the analysis. For both gonadal index and histopathology parameters (Table 7) analyses were conducted on paraffin-embedded tissues sectioned at a 5-micrometer thickness using microtome. After placing the sections onto slides, the paraffin was gently removed and the tissues hydrated using xylenes-ethanol series before being stained using a pentachrome staining procedure. Each slide was examined microscopically to determine the animal's sex and stage of gonadal development. Also the infection intensity of parasites, the occurrence and extent of tissue pathologies, and the intensity of diseases was recorded using quantitative or semi-quantitative measures. (end continuation)

NAME OF DERIVED VALUES Organic contaminants: chemicals with similar structural properties were summed and reported as "Totals" (O'Connor 1994 and 1996). The components of these totals are as follows: Total DDT = sum of concentrations of ortho and para forms of parent and metabolites 2,4'DDE; 4,4'DDE; 2,4'DDD; 4,4'DDD; 2,4'DDT and 4,4'DDT. Total Chlordane = sum of concentrations of four compounds alpha-chlordane, trans-nonachlor, heptachlor, heptachlorepoxide. Total Dieldrin = sum of concentrations of two compounds aldrin and dieldrin. Total Butyl tin = sum of concentrations of parent compound and metabolites monobutyltin, dibutyltin, tributyltin, [concentrations are in terms of tin]. Total PAHs = sum of measured PAHs. Total measured PCBs = PCB8/5, PCB103, PCB18, PCB28, PCB29, PCB31, PCB44, PCB45, PCB49, PCB52, PCB56/60, PCB66, PCB70, PCB74/61, PCB87/115, PCB95, PCB99, PCB101/90, PCB105, PCB110/77, PCB118, PCB128, PCB138, PCB146, PCB149/123, PCB151, PCB153/132/168 ,PCB156/171/202, PCB158, PCB170/190, PCB174, PCB180, PCB187, PCB183, PCB194, PCB195/208, PCB198, PCB201/173/157, PCB206, PCB209.

O'Connor, T.P. (1994). The national oceanic and atmospheric administration (NOAA) national status and trends mussel watch program: national monitoring of chemical contamination in the coastal United States. In Cr. Sothern and N.P. Ross (eds), Environmental statistics, assessment, and forecasting (pp. 331-349). Boca Raton, FL. Lewis Publishers O'Connor, T.P. (1996) Trends in chemical concentrations in mussels and oysters collected along the US coast from 1986 to 1993. Marine Environmental Research, 41, 183-200.