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Short Citation:
National Centers for Coastal Ocean Science, 2022: National Status and Trends, Benthic Surveillance Project Aryl Hydrocarbon Hydrolase (AHH) Data, 1988-1992, National Centers for Coastal Ocean Science, https://www.fisheries.noaa.gov/inport/item/39257.

Item Identification

Title: National Status and Trends, Benthic Surveillance Project Aryl Hydrocarbon Hydrolase (AHH) Data, 1988-1992, National Centers for Coastal Ocean Science
Short Name: bsahh_h
Status: Completed
Publication Date: 1992
Abstract:

In order to determine the current status of and detect any long-term trends in the environmental quality of U.S. nearshore waters, NOAA initiated the National Status and Trends program in 1984 with its National Benthic Surveillance Project. The primary objective of the Benthic Surveillance Project was to quantify concentrations of a suite of organic and inorganic contaminants in the livers of fish and surficial sediments from selected sites in the coastal and estuarine waters of the United States. In addition, the levels of certain indicators of the biological effects of these contaminants were measured. Incidences of visible lesions, including fin erosion, have been noted and histopathological examinations of various tissues have been carried out. Originally histopathological examinations determined the prevalence of any identifiable disease conditions in samples of liver, kidney, and gill tissue.In addition the Benthic Surveillance Project analyzed fish liver tissue sample for cytochrome P4501A (CYP1A) activities in liver via analysis for aryl hydrocarbon hydrolase (ahh). The induction of hepatic CYP1A activity is one of the earliest physiological changes detected after exposure to chemical contaminants. A number of studies have shown that measurement of these biochemical alterations combined with measurement of the above-mentioned injuries, give a more consistent assessment of contaminant exposure in indigenous fish that do single bioeffects measurements.

Purpose:

In response to concerns over environmental quality of the Nation's coastal and estuarine ecosystems, NOAA created the National Status and Trends (NSandT) Program in 1984. From 1984 through 1993, the Benthic Surveillance Project monitored chemical concentrations in the livers (and for metabolites of PAH's in the bile) of bottom-dwelling fish and in sediments at the sites of fish capture. The Benthic Surveillance Project also measured the biological effects of contaminant exposure, primarily as prevalence's of toxicopathic liver diseases.

Notes:

981

Keywords

Theme Keywords

Thesaurus Keyword
UNCONTROLLED
ISO 19115 Topic Category environment
None ahh
None aryl hydrocarbon hydrolase
None chemical
None coastal monitoring
None contaminant
None fish liver
None NCCOS Research Data Type > Field Observation
None NCCOS Research Priority > Long-term Monitoring
None NCCOS Research Priority > Stressors, Impacts, Mitigation, and Restoration (SIMR)
None NCCOS Research Topic > Bioeffects/Toxicity
None NCCOS Research Topic > Chemical Contaminants
None NCCOS Research Topic > Monitoring
None NSandT
None oceans
None pollutant

Spatial Keywords

Thesaurus Keyword
UNCONTROLLED
None Apalachicola Bay, St. George Island
None Arroyo Colorado, Arroyo City
None Baltimore Harbor, Brewerton Channel
None Barataria Bay, Barataria Pass
None Biscayne Bay, North Bay
None Bodega Bay, North
None Boston Harbor, Deer Island
None Boston Harbor, Hull Bay
None Boston Harbor, Mystic River
None Boston Harbor, Quincy Bay
None Buzzards Bay, West Island
None Calcasieu River, Prien Lake
None Calcasieu River, West Cove
None Cape Elizabeth, Richmond Island
None Cape Fear River, Horseshoe Shaol
None Charleston Harbor, South Channel
None Charlotte Harbor, Cape Haze
None Chesapeake Bay, Chester River
None Chesapeake Bay, James River
None Chesapeake Bay, Patuxent River
None Choctawhatchee Bay, Choctawhatchee Bay
None Columbia River, Desdemona Sands
None Coos Bay, North Bend
None Dana Point Harbor, Outside
None Dana Point, Inside Harbor
None Delaware Bay, Cherry Island Range
None Galveston Bay, Cedar Bayou
None Galveston Bay, Eagle Point
None Galveston Bay, East Bay
None Galveston Bay, Goat Islands
None Galveston Bay, Greens Bayou
None Galveston Bay, Morgans Point
None Great Bay, Intracoastal Waterway
None Hudson River, Englewood Cliffs
None Johns Bay, Pemaquid Neck
None Lake Pontchartrain, North Shore
None Lake Pontchartrain, South Shore
None Lavaca Bay, Point Comfort
None Long Island Sound, Lloyd Point
None Long Island Sound, Rocky Point
None Lower Laguna Madre, Laguna Heights
None Lower Laguna Madre, Long Island
None Lutak Inlet, Chilkoot River Mouth
None Massachusetts Bay, Plymouth Entrance
None Mission Bay, Outside
None Mississippi River Delta, Head of Passes
None Mississippi River Delta, Southeast Pass
None Mobile Bay, North Point
None Monterey Bay, Indian Head Beach
None Narragansett Bay, Prudence Island
None NCCOS Research Location > Geographic Area > Coastal Ocean
None NCCOS Research Location > Region > Alaska
None NCCOS Research Location > Region > East Coast
None NCCOS Research Location > Region > Gulf of Mexico
None NCCOS Research Location > Region > West Coast
None NCCOS Research Location > U.S. States and Territories > Alabama
None NCCOS Research Location > U.S. States and Territories > Alaska
None NCCOS Research Location > U.S. States and Territories > California
None NCCOS Research Location > U.S. States and Territories > Connecticut
None NCCOS Research Location > U.S. States and Territories > Delaware
None NCCOS Research Location > U.S. States and Territories > Florida
None NCCOS Research Location > U.S. States and Territories > Georgia
None NCCOS Research Location > U.S. States and Territories > Louisiana
None NCCOS Research Location > U.S. States and Territories > Maine
None NCCOS Research Location > U.S. States and Territories > Maryland
None NCCOS Research Location > U.S. States and Territories > Massachusetts
None NCCOS Research Location > U.S. States and Territories > Mississippi
None NCCOS Research Location > U.S. States and Territories > New Jersey
None NCCOS Research Location > U.S. States and Territories > New York
None NCCOS Research Location > U.S. States and Territories > North Carolina
None NCCOS Research Location > U.S. States and Territories > Oregon
None NCCOS Research Location > U.S. States and Territories > Rhode Island
None NCCOS Research Location > U.S. States and Territories > South Carolina
None NCCOS Research Location > U.S. States and Territories > Texas
None NCCOS Research Location > U.S. States and Territories > Virginia
None NCCOS Research Location > U.S. States and Territories > Washington
None New Bedford Harbor, Clarks Point
None Niantic Bay, Black Point
None Oceanside Harbor, Outside
None Pamlico Sound, Jones Bay
None Pascagoula River, Escatawpa River
None Pascagoula River, Pascagoula River
None Penobscot Bay, Colt Head Island
None Pensacola Bay, Pensacola Bay
None Prince William Sound, Port Valdez
None Puget Sound, Commencement Bay
None Puget Sound, Elliott Bay
None Puget Sound, Nisqually Reach
None Raritan Bay, East Reach
None Raritan Bay, Gravesend Bay
None Raritan Bay, Upper Bay
None Raritan Bay, West Reach
None Salem Harbor, Folger Point
None San Antonio Bay, Mosquito Point
None San Diego Bay, Harbor Island
None San Diego Bay, National City
None San Diego Bay, North
None San Diego Bay, Outside
None San Diego Bay, Shelter Island
None San Diego Bay, Twenty Eighth Street
None San Francisco Bay, Castro Creek
None San Francisco Bay, Hunters Point
None San Francisco Bay, Oakland Estuary
None San Francisco Bay, Redwood City
None San Francisco Bay, San Pablo Bay
None San Francisco Bay, Southampton Shoal
None San Luis Obispo Bay, San Luis Obispo Bay
None San Pedro Bay, Cerritos Channel
None San Pedro Bay, Long Beach
None San Pedro Bay, Mid Harbor
None San Pedro Bay, Outer Harbor
None San Pedro Bay, Seal Beach
None Santa Monica Bay, North
None Santa Monica Bay, South
None Santa Monica Bay, Southeast
None Santa Monica Bay, West
None Sapelo Sound, Dog Hammock
None Savannah River, Elba Island
None Skagway, Skagway River
None St. Andrews Bay, Military Point
None St. Johns River, Arlington Channel
None St. Lucie River, Stuart
None Tampa Bay, Northern Tampa Bay
None U.S. Exclusive Economic Zone

Physical Location

Organization: National Centers for Coastal Ocean Science
City: Silver Spring
State/Province: MD

Data Set Information

Data Set Scope Code: Data Set
Maintenance Frequency: None Planned
Data Presentation Form: Table (digital)
Distribution Liability:

None

Support Roles

Data Steward

CC ID: 462977
Date Effective From: 1992
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Distributor

CC ID: 462979
Date Effective From: 1992
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Metadata Contact

CC ID: 462980
Date Effective From: 1992
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Point of Contact

CC ID: 462978
Date Effective From: 1992
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Principal Investigator

CC ID: 462981
Date Effective From: 1992
Date Effective To:
Contact (Person): Piniak, Greg
Email Address: greg.piniak@noaa.gov

Extents

Currentness Reference: Publication Date

Extent Group 1

Extent Group 1 / Geographic Area 1

CC ID: 462984
W° Bound: -166.5
E° Bound: -67.333
N° Bound: 70.5
S° Bound: 25.615

Extent Group 1 / Time Frame 1

CC ID: 462983
Time Frame Type: Range
Start: 1988
End: 1992

Spatial Information

Spatial Representation

Representations Used

Vector: Yes

Access Information

Security Class: Unclassified
Data Access Constraints:

None

Data Use Constraints:

NOAA requests that all individuals who download NSandT data acknowledge the source of these data in any reports, papers, or presentations. If you publish these data, please include a statement similar to: "Some or all of the data described in this article were produced by NOAA through its National Status and Trends Program".

Distribution Information

Distribution 1

CC ID: 462986
Download URL: https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Distributor:
Description:

Live Data and Maps

File Type: XML

Distribution 2

CC ID: 462985
Download URL: https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Distributor:
Description:

Live Data and Maps

File Type: ASCII

URLs

Activity Log

Activity Log 1

CC ID: 463128
Activity Date/Time: 2016-11-16
Description:

Date that the source FGDC record was last modified.

Activity Log 2

CC ID: 463127
Activity Date/Time: 2017-04-05
Description:

Converted from FGDC Content Standards for Digital Geospatial Metadata (version FGDC-STD-001-1998) using 'fgdc_to_inport_xml.pl' script. Contact Tyler Christensen (NOS) for details.

Activity Log 3

CC ID: 584191
Activity Date/Time: 2017-09-13
Description:

Partial upload of Spatial Info section only.

Activity Log 4

CC ID: 716623
Activity Date/Time: 2018-02-08
Description:

Partial upload of Positional Accuracy fields only.

Technical Environment

Description:

Microsoft Windows 2000 Version 5.0 (Build 2195) Service Pack 4; ESRI ArcCatalog 8.3.0.800

Data Quality

Accuracy:

The quality of the analytical data generated by the NSandT Program is overseen by the QA Project component, which has been in operation since 1985 and is designed to document sampling and analytical procedures, and to reduce intra-laboratory and inter-laboratory variation. The QA Project documentation will facilitate comparisons among different monitoring programs with similar QA activities and thus will extend the temporal and spatial scale of such programs. To document laboratory expertise, the QA Project requires all NSandT laboratories to participate in a continuing series of intercomparison exercises utilizing a variety of materials.

Horizontal Positional Accuracy:

The same kind of field/site data have been supplied since the start of the Benthic Surveillance Project, in 1984. Original site coordinates were derived from Loran-C time conversions. Early sites information resulting from Loran-C was converted from time delay information to latitudes and longitudes. These earlier data may be suspect when sites were located close to large structures that could have interfered with accurate time delays. When GPS was first available the signal was intentionally degraded so earlier coordinate information, even if it resulted from GPS, is not as accurate as data would be today. Because fish are not sessile, fish trawls have been made along different tracks in the water body of interest. The latitude/ longitude coordinates provided in this file represent a site center and trawling occurs within a 1 km radius of this location.

Completeness Report:

Analytical protocols for the quantification of the NSandT organic contaminants were developed by MacLeod et al. (1984) at the NMFS (National Marine Fisheries Service) Northwest Fisheries Science Center in Seattle, WA. These methods were prescribed for all NMFS laboratories participating in the Benthic Surveillance Program, when it began in 1984. Three NMFS laboratories used these methods in 1984: Northeast Fisheries Science Center (NEFSC), Gloucester, MA; Southeast Fisheries Science Center (SEFSC), Charleston, SC; and Northwest Fisheries Science Center (NWFSC), Seattle WA. The philosophy associated with the development of exacting protocols for the quantification of organic contaminants was that the same analytical methods would increase the likelihood of data being comparable among laboratories. Even though interlaboratory comparisons were initiated at the start of the NSandT Program, it was felt that a method-driven QA and analytical effort for the quantification of organic contaminants was the best way to begin. In 1985, the protocols were updated by MacLeod et al., 1985. This method has been further edited and can be found in volume 1 of Technical Memorandum 71. The NSandT Mussel Watch Project (MWP) began in 1986. At that time, both the MWP and BSP laboratories were allowed to use any analytical method if it could be proven that the proposed alternate procedure was equal to or better than earlier MacLeod et al. (1984,1985) methods.

Conceptual Consistency:

Fish in the correct size range were dissected in the onboard laboratory immediately after collection. This ensured that a determination could be made regarding whether sufficient material had been collected and whether the sample material was of high quality. If either one of these criteria was not met, the opportunity existed to continue sample collection. Also, field dissection minimizes contamination problems associated with dissection of frozen fish samples. Frozen fish tissues when thawed, may lose their integrity and one tissue type may contaminate another (e.g., a liver sample could be contaminated by PAH metabolites from the bile duct).Because most fish were dissected onboard ship, a special effort was made to develop an environment as close to clean-room conditions as possible. All fish were dissected in positive pressure laminar flow hoods. Air was drawn into the laminar flow hood from above and filtered by a high efficiency particle attenuator (HEPA) filter before it passed over the fish samples. Stainless steel tools were used to dissect fish for organic analysis. Titanium tools were used to dissect fish for trace metal analyses because tools made of this element do not pose the problem of introducing nickel, chromium, and/or iron into the specimens to be analyzed. Specimens were analyzed for the latter three elements by the NSandT Program. After knives had been sharpened, and before dissections began at a new site or of a new species, the dissection equipment was thoroughly cleaned with detergent solution, rinsed extensively with tap water, rinsed in distilled or high-purity water (i.e., milli-Q or HPLC-grade water), rinsed with isopropanol (dichloromethane before 1990) under a fume hood, followed by a rinse with distilled water, and placed on a similarly cleaned Teflon cutting board that was allowed to air-dry in the laminar-flow hood. Between individual fish of the same species at the same site, the tools were rinsed with distilled water before any fluid or tissue had a chance to dry on the knife. Sexually mature fish were primarily used in this program, determined by size of each species.

Lineage

Process Steps

Process Step 1

CC ID: 462973
Description:

The primary collection apparatus was Otter trawls. Occasionally, fish were taken with hook and line, or with seine nets. These alternate collection methods were necessary because larger fish, such as older Atlantic croaker, were able to avoid an Otter trawl, or were found in untrawlable habitats such as shallow water, along marsh edges, and over oyster reefs. Fish liver tissue samples were analyzed for cytochrome P4501A (CYP1A) activities in liver via analysis for aryl hydrocarbon hydrolase (ahh).The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples, and analyzing standard reference materials.Process Date Range is 1988 - 1992

Process Date/Time: 1992-01-01 00:00:00

Child Items

Rubric scores updated every 15m

Rubric Score Type Title
Entity Dataset Parameters for Benthic Surveillance Aryl Hydrocarbon Hydrolase data

Catalog Details

Catalog Item ID: 39257
GUID: gov.noaa.nmfs.inport:39257
Metadata Record Created By: Tyler Christensen
Metadata Record Created: 2017-04-05 12:51+0000
Metadata Record Last Modified By: SysAdmin InPortAdmin
Metadata Record Last Modified: 2022-08-09 17:11+0000
Metadata Record Published: 2018-02-08
Owner Org: NCCOS
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Last Review Date: 2018-02-08
Metadata Review Frequency: 1 Year
Metadata Next Review Date: 2019-02-08