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Short Citation:
National Centers for Coastal Ocean Science, 2022: National Status and Trends, Benthic Surveillance Project DNA-Xenobiotic Adducts Data, 1991, National Centers for Coastal Ocean Science, https://www.fisheries.noaa.gov/inport/item/39258.

Item Identification

Title: National Status and Trends, Benthic Surveillance Project DNA-Xenobiotic Adducts Data, 1991, National Centers for Coastal Ocean Science
Short Name: bsdna_h
Status: Completed
Publication Date: 1992
Abstract:

In order to determine the current status of and to detect any long-term trends in the environmental quality of U.S. nearshore waters, NOAA initiated the National Status and Trends program in 1984 with its National Benthic Surveillance Project. The primary objective of the Benthic Surveillance Project was to quantify concentrations of a suite of organic and inorganic contaminants in the livers of fish and surficial sediments from selected sites in the coastal and estuarine waters of the United States. In addition, the levels of certain indicators of the biological effects of these contaminants were measured. Incidences of visible lesions, including fin erosion, have been noted and histopathological examinations of various tissues have been carried out. Originally histopathological examinations determined the prevalence of any identifiable disease conditions in samples of liver, kidney, and gill tissue.In addition, the Benthic Surveillance Project tested fish livers from 30 locations in 1991 to identify the concentrations of DNA xenobiotic adducts. DNA xenobiotic adducts are a bioindicator of exposure to genotoxic compounds. Measurement of biological markers such as DNA xenobiotic adducts have been used to improve the evaluation of contaminant exposure and responses in indigenous fish, and thus reinforce the connection between exposure to toxic chemicals and the observed injuries.

Purpose:

In response to concerns over environmental quality of the Nation's coastal and estuarine ecosystems, NOAA created the National Status and Trends (NSandT) Program in 1984. From 1984 through 1993, the Benthic Surveillance Project monitored chemical concentrations in the livers (and for metabolites of PAH's in the bile) of bottom-dwelling fish and in sediments at the sites of fish capture. The Benthic Surveillance Project also measured the biological effects of contaminant exposure, primarily as prevalence's of toxicopathic liver diseases.

Notes:

982

Keywords

Theme Keywords

Thesaurus Keyword
UNCONTROLLED
ISO 19115 Topic Category environment
None Coastal Monitoring
None deoxyribonucleic acid
None DNA
None DNA abnormalities
None DNA adducts
None DNA xenobiotic adducts
None genotoxic exposure
None NCCOS Research Data Type > Field Observation
None NCCOS Research Priority > Long-term Monitoring
None NCCOS Research Priority > Stressors, Impacts, Mitigation, and Restoration (SIMR)
None NCCOS Research Topic > Bioeffects/Toxicity
None NCCOS Research Topic > Chemical Contaminants
None NCCOS Research Topic > Monitoring
None NSandT
None oceans
None xenobiotic deoxyribonucleic acid adducts

Spatial Keywords

Thesaurus Keyword
UNCONTROLLED
None Baltimore Harbor, Brewerton Channel
None Boston Harbor, Deer Island
None Cape Elizabeth, Richmond Island
None Cape Fear River, Horseshoe Shaol
None Charlotte Harbor, Cape Haze
None Chesapeake Bay, Chester River
None Chesapeake Bay, James River
None Chesapeake Bay, Patuxent River
None Choctawhatchee Bay, Choctawhatchee Bay
None Dana Point Harbor, Outside
None Delaware Bay, Cherry Island Range
None Johns Bay, Pemaquid Neck
None Lower Laguna Madre, Laguna Heights
None Lower Laguna Madre, Long Island
None NCCOS Research Location > Geographic Area > Coastal Ocean
None NCCOS Research Location > Region > Alaska
None NCCOS Research Location > Region > East Coast
None NCCOS Research Location > Region > Gulf of Mexico
None NCCOS Research Location > Region > West Coast
None NCCOS Research Location > U.S. States and Territories > Alabama
None NCCOS Research Location > U.S. States and Territories > Alaska
None NCCOS Research Location > U.S. States and Territories > California
None NCCOS Research Location > U.S. States and Territories > Connecticut
None NCCOS Research Location > U.S. States and Territories > Delaware
None NCCOS Research Location > U.S. States and Territories > Florida
None NCCOS Research Location > U.S. States and Territories > Georgia
None NCCOS Research Location > U.S. States and Territories > Louisiana
None NCCOS Research Location > U.S. States and Territories > Maine
None NCCOS Research Location > U.S. States and Territories > Maryland
None NCCOS Research Location > U.S. States and Territories > Massachusetts
None NCCOS Research Location > U.S. States and Territories > Mississippi
None NCCOS Research Location > U.S. States and Territories > New Jersey
None NCCOS Research Location > U.S. States and Territories > New York
None NCCOS Research Location > U.S. States and Territories > North Carolina
None NCCOS Research Location > U.S. States and Territories > Oregon
None NCCOS Research Location > U.S. States and Territories > Rhode Island
None NCCOS Research Location > U.S. States and Territories > South Carolina
None NCCOS Research Location > U.S. States and Territories > Texas
None NCCOS Research Location > U.S. States and Territories > Virginia
None NCCOS Research Location > U.S. States and Territories > Washington
None New Bedford Harbor, Clarks Point
None Pascagoula River, Escatawpa River
None Pensacola Bay, Pensacola Bay
None Raritan Bay, East Reach
None Raritan Bay, Upper Bay
None Salem Harbor, Folger Point
None San Antonio Bay, Mosquito Point
None San Diego Bay, North
None San Diego Bay, Twenty Eighth Street
None San Francisco Bay, Hunters Point
None San Francisco Bay, Southampton Shoal
None San Pedro Bay, Outer Harbor
None Sapelo Sound, Dog Hammock
None St. Andrews Bay, Military Point
None St. Johns River, Arlington Channel
None St. Lucie River, Stuart
None U.S. Exclusive Economic Zone

Physical Location

Organization: National Centers for Coastal Ocean Science
City: Silver Spring
State/Province: MD

Data Set Information

Data Set Scope Code: Data Set
Maintenance Frequency: None Planned
Data Presentation Form: Table (digital)
Distribution Liability:

None

Support Roles

Data Steward

CC ID: 463134
Date Effective From: 1992
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Distributor

CC ID: 463136
Date Effective From: 1992
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Metadata Contact

CC ID: 463137
Date Effective From: 1992
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Point of Contact

CC ID: 463135
Date Effective From: 1992
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Principal Investigator

CC ID: 463138
Date Effective From: 1992
Date Effective To:
Contact (Person): Piniak, Greg
Email Address: greg.piniak@noaa.gov

Extents

Currentness Reference: Publication Date

Extent Group 1

Extent Group 1 / Geographic Area 1

CC ID: 463141
W° Bound: -166.5
E° Bound: -67.3
N° Bound: 70.5
S° Bound: 25.6

Extent Group 1 / Time Frame 1

CC ID: 463140
Time Frame Type: Discrete
Start: 1991

Spatial Information

Spatial Representation

Representations Used

Vector: Yes

Access Information

Security Class: Unclassified
Data Access Constraints:

None

Data Use Constraints:

NOAA requests that all individuals who download NSandT data acknowledge the source of these data in any reports, papers, or presentations. If you publish these data, please include a statement similar to: "Some or all of the data described in this article were produced by NOAA through its National Status and Trends Program".

Distribution Information

Distribution 1

CC ID: 463143
Download URL: https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Distributor:
Description:

Live Data and Maps

File Type: XML

Distribution 2

CC ID: 463142
Download URL: https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Distributor:
Description:

Live Data and Maps

File Type: ASCII

URLs

Activity Log

Activity Log 1

CC ID: 463219
Activity Date/Time: 2016-11-16
Description:

Date that the source FGDC record was last modified.

Activity Log 2

CC ID: 463218
Activity Date/Time: 2017-04-05
Description:

Converted from FGDC Content Standards for Digital Geospatial Metadata (version FGDC-STD-001-1998) using 'fgdc_to_inport_xml.pl' script. Contact Tyler Christensen (NOS) for details.

Activity Log 3

CC ID: 584193
Activity Date/Time: 2017-09-13
Description:

Partial upload of Spatial Info section only.

Activity Log 4

CC ID: 716624
Activity Date/Time: 2018-02-08
Description:

Partial upload of Positional Accuracy fields only.

Technical Environment

Description:

Microsoft Windows 2000 Version 5.0 (Build 2195) Service Pack 3; ESRI ArcCatalog 8.3.0.800

Data Quality

Horizontal Positional Accuracy:

The same kind of field/site data have been supplied since the start of the Benthic Surveillance Project, in 1984. Original site coordinates were derived from Loran-C time conversions. Early sites information resulting from Loran-C was converted from time delay information to latitudes and longitudes. These earlier data may be suspect when sites were located close to large structures that could have interfered with accurate time delays. When GPS was first available the signal was intentionally degrades so earlier coordinate information, even if it resulted from GPS, is not as accurate as data would be today. Because fish are not sessile, fish trawls have been made along different tracks in the water body of interest. The latitude/longitude coordinates provided in this file represent a nominal site center and trawling occurs within a 1 km radius of this location.

Completeness Report:

The quality of the analytical data generated by the NSandT Program is overseen by the QA Project component, which has been in operation since 1985 and is designed to document sampling and analytical procedures, and to reduce intralaboratory and interlaboratory variation. The QA Project documentation will facilitate comparisons between different monitoring programs with similar QA activities and thus will extend the temporal and spatial scale of such programs. To document laboratory expertise, the QA Project requires all NSandT laboratories to participate in a continuing series of intercomparison exercises utilizing a variety of materials. The organic analytical intercomparison exercises are coordinated by the NIST, and the inorganic exercises by the National Research Council (NRC) of Canada. Details of quality assurance for the Benthic Surveillance Program can be found in Lauenstein and Cantillo, 1993.

Conceptual Consistency:

The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples and analyzing standard reference materials. To assess the reproducibility of results every tissue sample was analyzed in duplicate.

Lineage

Process Steps

Process Step 1

CC ID: 463130
Description:

The primary collection apparatus was Otter trawls. Occasionally, fish were taken with hook and line, or with seine nets. These alternate collection methods were necessary because larger fish, such as older Atlantic croaker, were able to avoid an Otter trawl, or were found in untrawlable habitats such as shallow water, along marsh edges, and over oyster reefs. Fish liver tissue samples were analyzed for DNA-xenobiotic adducts in liver via Phosphorus 32-postlabeling assay to indicate genotoxic exposure. The analysis of DNA adducts by Phosphorus 32-postlabeling is a multistep process involving a series of biochemical reactions. First, DNA is hydrolyzed enzymatically to 3'monophosphates. The digest is then enriched in xenobiotic-modified mononucleotides by the selective removal of normal nucleotides. Following the enrichment step the adducted DNA is enzymatically labeled at the 5'-hydroxyl position with [Phosphorus 32] phosphate to form [5'-Phosphorus 32] deoxyribonucleoside 3',5'-bisphosphates. Separation of the Phosphorus 32-labeled adducts is usually accomplished by two-dimensional, thin-layer chromatography (TLC) on polyethyleneimine (PEI)-modified cellulose sheets. Autoradiography or storage phosphor imaging (Reichert et al., 1992) is then used to locate the radiolabeled adducts on the chromatogram (Reichert and French, 1994).The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples and analyzing standard reference materials.

Process Date/Time: 1991-01-01 00:00:00

Child Items

Rubric scores updated every 15m

Rubric Score Type Title
Entity Dataset Parameters for Benthic Surveillance DNA Xenobiotic Adducts data

Catalog Details

Catalog Item ID: 39258
GUID: gov.noaa.nmfs.inport:39258
Metadata Record Created By: Tyler Christensen
Metadata Record Created: 2017-04-05 12:51+0000
Metadata Record Last Modified By: SysAdmin InPortAdmin
Metadata Record Last Modified: 2022-08-09 17:11+0000
Metadata Record Published: 2018-02-08
Owner Org: NCCOS
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Last Review Date: 2018-02-08
Metadata Review Frequency: 1 Year
Metadata Next Review Date: 2019-02-08