AFSC/NMML/CCEP: Sampling locations for harbor seal genetics in Washington and British Columbia

Using skin samples from 777 unweaned pups collected in 9 different regions in WA state and British Columbia (WA Coastal Estuaries, WA North Coast, British Columbia, Boundary Bay, San Juan Islands, Smith/Minor Islands, Dungeness Spit, Hood Canal, South Puget Sound), the California Current Ecosystem Program at the Marine Mammal Laboratory/AFSC tested levels of genetic variation using mtDNA and 9 microsatellite loci. For both mtDNA and microsatellites we found the same four groupings. Previously, harbor seals were managed as two stocks: coastal and Inland waters. The data indicated that the harbor seals should be managed as four stocks: one on the Coast and three stocks in the Inland Waters. An additional 92 skin samples were collected opportunistically from non-pups. They have not yet been analyzed.

The CCEP of MML collected genetic samples from pups to determine stocks of harbor seals on Washington Coast and Inland Waters. Genetic samples from other age classes were also collected.

Cite data as: Huber, H.R., B.R. Dickerson, D.M. Lambourn, S.J.Jeffries (2016). Sampling location for harbor seal genetics in Washington and British Columbia from 1993-08-25 to 2009-09-23 (NCEI Accession 0148458). NOAA National Centers for Environmental Information.

The user is responsible for the results of any application of this data for other than its intended purpose. NOAA denies liability if the data are misused. Applications or inferences derived from the data should be carefully considered for accuracy. Whereas every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by any errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty.

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The dataset is archived with the NOAA National Centers for Environmental Information (NCEI). Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these data is suggested. Communication and collaboration with dataset authors is strongly encouraged prior to use.

There are no legal restrictions on access to the data. They reside in public domain and can be freely distributed.

User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. Data must be cited as: Huber, H.R., B.R. Dickerson, D.M. Lambourn, S.J.Jeffries (2016). Sampling location for harbor seal genetics in Washington and British Columbia from 1993-08-25 to 2009-09-23 (NCEI Accession 0148458). NOAA National Centers for Environmental Information.

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Metadata are subject to revision at any time and information within the metadata record should not be cited without author consent. Whereas every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by any errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty.

MS Excel

Data were proofed for consistency and logic in the field and in the laboratory.

Data are not automatically processed

IT Security and Contingency Plan for the system establishes procedures and applies to the functions, operations, and resources necessary to recover and restore data as hosted in the Western Regional Support Center in Seattle, Washington, following a disruption.

Capture methods were by beach seine or tangle net or 'run and grab;' methods are described in Jeffries et al. 1993. Genetic samples were acquired using a 6mm skin punch. Samples not collected by MML were collected by Wolf Hollow Wildlife Rehabilitation Center (San Juan Island, WA), Sarvey Wildlife Center (Arlington, WA), Washington Fish and Wildlife Department (Tacoma, WA) necropsies, and Cascadia Research Collective (Olympia, WA) necropsies. Samples were analyzed at Southwest Fisheries Science Center (La Jolla, CA), Northwest Fisheries Science Center (Seattle, WA), and at MERL/AFSC (Seattle, WA).