Short Citation:
Pacific Islands Fisheries Science Center, 2021: Calcification rates of crustose coralline algae (CCA) derived from Calcification Accretion Units (CAUs) deployed at coral reef sites in Timor-Leste from 2012 to 2014, https://www.fisheries.noaa.gov/inport/item/46162.

Item Identification

Title: Calcification rates of crustose coralline algae (CCA) derived from Calcification Accretion Units (CAUs) deployed at coral reef sites in Timor-Leste from 2012 to 2014
Short Name: Timor: CAUs NEW
Status: Completed
Publication Date: 2017
Abstract:

The calcification rate data described here were collected by the NOAA Coral Reef Ecosystem Program (CREP) from calcification accretion units, or CAUs, moored for two years at fixed climate survey sites and located on hard bottom shallow water (< 15 m) habitats in Timor-Leste, in accordance with protocols developed by Price et al. (2012). Five CAUs were deployed at each survey site. Climate sites were established in Timore-Leste by CREP in October 2012 to establish ecological baselines for climate change by measuring multiple features of the coral reef environment (in addition to the data described herein) over time.

CAUs, constructed in-house by CREP, are composed of two 10 x 10 cm flat, square, gray PVC plates, stacked 1 cm apart, and are attached to the benthos by SCUBA divers using stainless steel threaded rods. Deployed on the seafloor for a period of time, calcareous organisms, primarily crustose coralline algae and encrusting corals, recruit to these plates and accrete/calcify carbonate skeletons over time. By measuring the change in weight of the CAUs, the reef carbonate accretion rate can be calculated for that time period, measured in grams per centimeter per year.

Laboratory experiments reveal calcification rates of crustose coralline algae (CCA) are strongly correlated to seawater aragonite saturation state. Predictions of reduced coral calcification rates, due to ocean acidification, suggest that coral reef communities will undergo ecological phase shifts as calcifying organisms are negatively impacted by changing seawater chemistry. CAUs are used by CREP to assess the current effects of changes in seawater carbonate chemistry on calcification and accretion rates of calcareous and fleshy algae.

These calcification rate data for Timor-Leste, along with other data collected at the climate survey sites (water temperature and chemistry, invertebrate biodiversity, and benthic cover, all archived separately), serve as a baseline for detecting changes associated with changing seawater chemistry and can be used to help scientists assess and understand how Timor-Leste's coral reefs are responding to ocean acidification. The data can be accessed online via the NOAA National Centers for Environmental Information (NCEI) Ocean Archive.

Purpose:

In 2011, NOAA and the United States Agency for International Development (USAID) Timor-Leste Mission formed a 5-year bi-lateral partnership agreement from 2012 to 2016, in support of the Government of Timor-Leste—particularly the Ministry of Agriculture and Fisheries (MAF)—by addressing significant information gaps in their nearshore marine ecosystems. Specifically, MAF asked NOAA to address the following questions:

1.Where are the nearshore marine resources?

2.What are the nearshore marine resources?

3.How are they changing over time and what threats are potentially causing these changes?

4.What approaches are needed to manage and conserve the nearshore marine resources?

With financial support from USAID and in-kind support from the NOAA Coral Reef Conservation Program (CRCP), the NOAA Coral Reef Ecosystem Program (CREP) conducted baseline ecosystem assessment surveys to inform ecosystem-based management planning in Timor-Leste. The primary activities conducted by NOAA to answer these questions included:

1.satellite mapping of nearshore habitats,

2.conducting coral reef ecosystem assessments,

3.establishing ecological baselines for climate change, and

4.building management capacity by developing a spatial data framework

The calcification rate data described herein resulted from the third activity to establish ecological baselines for climate change.

Other Citation Details:

Pacific Islands Fisheries Science Center. 2017. Interdisciplinary baseline ecosystem assessment surveys to inform ecosystem-based management planning in Timor-Leste: Final Report. Pacific Islands Fisheries Science Center, PIFSC Special Publication, SP-17-002, 234 p. https://doi.org/10.7289/V5/SP-PIFSC-17-002.

Supplemental Information:

As the survey methods described and used herein are also implemented as part of the NOAA Pacific Reef Assessment and Monitoring Program (Pacific RAMP)—an ecosystem-scale interdisciplinary coral reef monitoring program—the data from Timor-Leste are directly comparable to and informed by data collected by NOAA-CREP throughout the U.S. Pacific Islands and Territories. Furthermore, the methods used for establishing ecological baselines for climate change in Timor-Leste (Chapter 4) are being adopted and implemented at 21 sites across eight member states of the Intergovernmental Oceanographic Commission within the Western Pacific (WESTPAC) region. These data provide a foundation for comparing the ecological baselines under current and future stresses associated with climate change in the Coral Triangle region.

Keywords

Theme Keywords

Thesaurus Keyword
CoRIS Discovery Thesaurus Numeric Data Sets > Calcification Rate
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Aquatic Habitat > Reef Habitat
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Algal Cover
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Algal Growth > Calcification Rate
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Calcareous Macroalgae
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Crustose Coralline Algae
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Encrusting Macroalgae
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Fleshy Macroalgae
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Reef Monitoring and Assessment
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Reef Monitoring and Assessment > Calcification Accretion Unit (CAU)
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Baseline studies
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > In Situ Biological
CoRIS Theme Thesaurus EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs
CoRIS Theme Thesaurus EARTH SCIENCE > Oceans > Ocean Chemistry > Calcification
CoRIS Theme Thesaurus EARTH SCIENCE > Oceans > Ocean Chemistry > Carbonate Chemistry
CoRIS Theme Thesaurus EARTH SCIENCE > Oceans > Ocean Chemistry > Ocean Acidification
ISO 19115 Topic Category 002
ISO 19115 Topic Category biota
NODC DATA TYPES THESAURUS CALCIFICATION
NODC Observation Types Thesaurus laboratory analyses
NODC Project Names Thesaurus CORAL REEF STUDIES
NODC Project Names Thesaurus Coral Triangle Initiative
NODC Submitting Institution Names Thesaurus US DOC; NOAA; NMFS; Pacific Islands Fisheries Science Center; Ecosystem Sciences Division; Coral Reef Ecosystem Program
None calcification accretion unit
None Calcification Plate
None CAU
None Coral Reef Ecosystem Division
None Coral Reef Ecosystem Program
None CRED
None CREP
None Ocean Acidification
None Pacific Islands Fisheries Science Center
None PIFSC
None Settling Plate
None United States Agency for International Development
None USAID

Spatial Keywords

Thesaurus Keyword
CoRIS Place Thesaurus COUNTRY/TERRITORY > Democratic Republic of Timor-Leste (East Timor) (09S126E0001)
CoRIS Place Thesaurus OCEAN BASIN > Indian Ocean > Timor Sea > Democratic Republic of Timor-Leste (East Timor) (09S126E0001)
NODC Sea Area Names Thesaurus Indian Ocean
NODC Sea Area Names Thesaurus Timor Sea
None Atauro
None Baucau
None Bobonaro
None Dili
None Lautem
None Manatuto
None Timor-Leste
None Vila MPA

Physical Location

Organization: Pacific Islands Fisheries Science Center
City: Honolulu
State/Province: HI
Country: USA

Data Set Information

Data Set Scope Code: Data Set
Data Set Type: CSV Files
Maintenance Frequency: None Planned
Data Presentation Form: Table (digital)
Entity Attribute Overview:

Dataset includes field metadata (location, mission, dates deployed and recovered) and information about the CAUs (serial number and id), and the calcification rates (measured in grams per centimeter per year) for each CAU unit (up to 5 CAUs per site), as well as the mean calcification rate per site (including the standard deviation and standard error)

Distribution Liability:

While every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty.

Data Set Credit: PIFSC Coral Reef Ecosystem Program and funded by the United States Agency for International Development

Support Roles

Data Steward

CC ID: 569518
Date Effective From: 2017
Date Effective To:
Contact (Person): Young, Charles W
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: charles.young@noaa.gov
Contact Instructions:

Email preferred

Data Steward

CC ID: 569516
Date Effective From: 2012
Date Effective To: 2016
Contact (Person): Misa, Paula WL
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: paula.misa@noaa.gov
Phone: 808-725-5458
Contact Instructions:

Email preferred

Distributor

CC ID: 569517
Date Effective From: 2017
Date Effective To:
Contact (Organization): National Centers for Environmental Information - Silver Spring, Maryland (NCEI-MD)
Address: NOAA/NESDIS E/OC SSMC3, 4th Floor, 1351 East-West Highway
Silver Spring, MD 20910-3282
Phone: (301) 713-3277
Contact Instructions:

Email preferred

Distributor

CC ID: 569512
Date Effective From: 2012
Date Effective To: 2017
Contact (Person): Kanemura, Troy T
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: troy.kanemura@noaa.gov
Phone: (808)725-5422
Contact Instructions:

Email preferred

Metadata Contact

CC ID: 569511
Date Effective From: 2012
Date Effective To:
Contact (Person): DesRochers, Annette M
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: annette.desrochers@noaa.gov
Phone: (808)725-5461
Business Hours: 8 am - 5 pm
Contact Instructions:

Email preferred

Originator

CC ID: 569513
Date Effective From: 2012
Date Effective To:
Contact (Organization): Pacific Islands Fisheries Science Center (PIFSC)
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Phone: 808-725-5300
URL: http://www.pifsc.noaa.gov
Business Hours: 8:00 a.m. - 4:30 p.m.

Point of Contact

CC ID: 569514
Date Effective From: 2015
Date Effective To:
Contact (Person): Oliver, Thomas
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: thomas.oliver@noaa.gov
Phone: (808)725-5444
Contact Instructions:

Email preferred

Point of Contact

CC ID: 569515
Date Effective From: 2012
Date Effective To: 2014
Contact (Person): Young, Charles W
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: charles.young@noaa.gov
Contact Instructions:

Email preferred

Extents

Currentness Reference: Ground Condition

Extent Group 1

Extent Description:

Timor-Leste

Extent Group 1 / Geographic Area 1

CC ID: 700550
W° Bound: 125.01327
E° Bound: 127.31222
N° Bound: -8.22441
S° Bound: -8.85329
Description

Extent of climate survey sites in Timor-Leste.

Extent Group 1 / Time Frame 1

CC ID: 700551
Time Frame Type: Range
Start: 2012-10-15
End: 2014-10-09
Description:

Time frame of calcification rate data for Timor-Leste

Access Information

Security Class: Unclassified
Security Classification System:

Not applicable

Security Handling Description:

Not applicable

Data Access Policy:

Coral Reef Ecosystem Program (CREP) Data Sharing Recommendations, version 9.0 updated August 12, 2015:

CREP welcomes the opportunity to collaborate on research issues contributing to the scientific basis for better management of marine ecosystems. CREP has a very diverse set of field activities that generates large volumes of data using an array of data collection protocols.

The following recommendations are for your consideration as you use this data:

1) Data analyses should take all field exigencies into account. The most effective way to do this would be active collaboration with CREP principal investigators.

2) In all presentations, product releases, or publications using data generated by CREP, proper acknowledgement of both CREP and the individuals responsible for data collection is expected. Citing the DOI (if available) is preferred, a non-DOI example is listed below.

3) If you collect or generate data for the same study areas, CREP requests that you share relevant information on complimentary data collections.

4) Those receiving data are strongly urged to inform the CREP Data Management Team of any errors and discrepancies that are discovered during the course of using these data. They are further urged to bring to the attention of the Team all problems and difficulties encountered in using these data. This information is necessary in order to improve the collections and to facilitate more efficient and economical data processing and retrieval. The users are asked to supply copies of any missing data that may be located, and to provide information as to significant subsets and special aggregations of data that are developed in using the material provided.

Example citation:

"This publication makes use of data products provided by the Coral Reef Ecosystem Program (CREP), Pacific Islands Fisheries Science Center (PIFSC), National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA), with funding support from the United States Agency for International Development. The analysis and interpretations presented here are solely that of the current authors”

Data Access Procedure:

Data can be accessed online via the NOAA National Centers for Environmental Information (NCEI) Ocean Archive.

Data Access Constraints:

None

Data Use Constraints:

Please cite NOAA Coral Reef Ecosystem Program (CREP) when using the data.

Suggested Citation:

Coral Reef Ecosystem Program; Pacific Islands Fisheries Science Center (2017). Calcification rates of crustose coralline algae (CCA) derived from Calcification Accretion Units (CAUs) deployed at coral reef sites in Timor-Leste from 2012 to 2014. NOAA's National Center for Environmental Information, https://www.fisheries.noaa.gov/inport/item/46162.

Metadata Access Constraints:

None

Metadata Use Constraints:

None

Distribution Information

Distribution 1

CC ID: 700547
Download URL: http://accession.nodc.noaa.gov/0170031
Distributor: National Centers for Environmental Information - Silver Spring, Maryland (NCEI-MD) (2017 - Present)
File Name: CAU_CalcificationRate_Site_Timor_2014.csv
Description:

Mean calcification rate data for each survey site derived from calcification accretion units (CAUs) deployed and recovered by the NOAA Coral Reef Ecosystem Program at sites in Timor-Leste from 2012 to 2014. Five units were deployed at each survey site.

File Date/Time: 2017-06-01 00:00:00
File Type: csv (comma-separated values)

Distribution 2

CC ID: 569523
Download URL: http://accession.nodc.noaa.gov/0170031
Distributor: National Centers for Environmental Information - Silver Spring, Maryland (NCEI-MD) (2017 - Present)
File Name: CAU_CalcificationRate_Unit_Timor_2014.csv
Description:

Calcification rate data derived from calcification accretion units (CAUs) deployed and recovered by the NOAA Coral Reef Ecosystem Program at sites in Timor-Leste from 2012 to 2014.

File Date/Time: 2017-06-06 00:00:00
File Type: csv (comma-separated values)

Distribution 3

CC ID: 569520
Download URL: https://www.nodc.noaa.gov/archive/arc0081/0137093/1.1/data/0-data/CRED_OA_CAU_2010-2014/Documentation/CAU1_Assembly_Deployment_Recovery_CRED_SOP2015.pdf
Distributor: National Centers for Environmental Information - Silver Spring, Maryland (NCEI-MD) (2017 - Present)
File Name: CAU1_Assembly_Deployment_Recovery_CRED_SOP2015.pdf
Description:

NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Assembly, Deployment, and Recovery Standard Operating Procedure (2015)

File Date/Time: 2015-09-10 00:00:00
File Type: PDF
File Size: 786 KB

Distribution 4

CC ID: 569521
Download URL: https://www.nodc.noaa.gov/archive/arc0081/0137093/1.1/data/0-data/CRED_OA_CAU_2010-2014/Documentation/CAU2_Sample%20Processing_CRED_SOP_2015.pdf
Distributor: National Centers for Environmental Information - Silver Spring, Maryland (NCEI-MD) (2017 - Present)
File Name: CAU2_Sample Processing_CRED_SOP_2015.pdf
Description:

NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Laboratory Analysis Standard Operating Procedure (2015)

File Date/Time: 2015-09-10 00:00:00
File Type: PDF
File Size: 811 KB

URLs

URL 1

CC ID: 569524
URL: https://www.coris.noaa.gov/activities/projects/timor-leste/
URL Type:
Online Resource
Description:

Timor-Leste project portal on the NOAA Coral Reef Information Service website where NOAA's final report for Timor-Leste and the datasets generated by NOAA CREP as part of the project can be downloaded.

URL 2

CC ID: 569525
URL: https://www.pifsc.noaa.gov/cred/coral_triangle_initiative.php
URL Type:
Online Resource
File Resource Format: PHP
Description:

Information about the NOAA Coral Reef Ecosystem Program's activities in the Coral Triangle.

URL 3

CC ID: 569526
URL: https://pifscblog.wordpress.com/2012/11/20/final-count-timor-leste/
URL Type:
Online Resource
Description:

PIFSC blog post, Nov 2012: The final count: summary of mission to Timor-Leste in 2012

URL 4

CC ID: 569527
URL: http://www.pifsc.noaa.gov/cred/img/calcium_accretion_unit_after_two_years_sm.jpg
URL Type:
Browse Graphic
File Resource Format: JPEG
Description:

A CAU two years after deployment.

URL 5

CC ID: 569528
URL: https://pifscblog.wordpress.com/2014/09/30/timor-leste-atauro/
URL Type:
Online Resource
Description:

PIFSC blog post, Sept 2014: Scientists return to Timor-Leste for reef monitoring mission

URL 6

CC ID: 700552
URL: https://www.pifsc.noaa.gov/cred/ocean_acidification.php
URL Type:
Online Resource
Description:

PIFSC CREP website on ocean acidification and related methods.

Technical Environment

Description:

PIFSC Oracle database view: V_CALCIFICATION_RATE_BY_UNIT

Data Quality

Accuracy:

Prior to processing the calcification accretion unit (CAU) samples, laboratory analysts are trained to properly use relevant lab equipment (analytical balance, vacuum filtration pump, etc.) and are familiarized with the data entry tool/spreadsheet. Laboratory analysts are also required to go through the CAU Processing Standard Operating Procedure and other training materials.

Completeness Measure:

Calcification Accretion Units (CAUs) that have been deployed may not have been recovered due to logistical constraints of the following mission or could not be found when divers returned to the site. This can be quantified by comparing the number of recovered units to the number of units originally deployed (5 units per site).

Completeness Report:

The calcification rate and ratio of calcified materials to fleshy materials are determined for each Calcification Accretion Unit (CAU) recovered by the NOAA Coral Reef Ecosystem Program (CREP). disassembly process. CAUs not recovered are not part of this dataset, and therefore, there may be less than 5 observations per survey site.

Conceptual Consistency:

The sampling procedure is based on the protocols developed by Price et al. 2012 with slight modifications. Specifically, the following changes were implemented in 2013: 1) camera settings (adjusted to fit laboratory light conditions), and 2) use of freshwater in lieu of seawater during plate photo-documentation (seawater was not readily available in the new facility). Further, the processing procedures were also modified in 2013 such that the acceptable criteria for samples to be considered completely dry was set to 0.1 grams instead of 0.2 grams—that is, if two subsequent dry weights do not fall within the identified acceptable range (0.1 grams), the drying process will continue until this criteria is met.

Quality Control Procedures Employed:

Prior to processing a batch of CAUs recovered from a region, laboratory analysts inventory the recovered Calcification Accretion Units (CAUs) to ensure all units are accounted for and all serial numbers assigned to each unit match field data records. The Data Manager additionally performs quality control checks on the site deployment data and the recovery data for further accuracy. Once all CAUs from the region have been processed, the dataset is checked by the analysts to ensure: 1) all CAU plates have been processed, 2) there are no missing cell values, and 3) calculations for net weight of calcified materials are correct. The dataset is then turned over to the Data Manager for further quality control procedures and data ingestion into the NOAA Coral Reef Ecosystem Program's master database.

Data Management

Have Resources for Management of these Data Been Identified?: Yes
Approximate Percentage of Budget for these Data Devoted to Data Management: Unknown
Do these Data Comply with the Data Access Directive?: Yes
Is Access to the Data Limited Based on an Approved Waiver?: No
Approximate Delay Between Data Collection and Dissemination: Unknown
Actual or Planned Long-Term Data Archive Location: NCEI-MD
Approximate Delay Between Data Collection and Archiving: Unknown
How Will the Data Be Protected from Accidental or Malicious Modification or Deletion Prior to Receipt by the Archive?:

NOAA IRC and NOAA Fisheries ITS resources and assets.

Lineage

Lineage Statement:

Assembled calcification accretion units (CAUs) are attached to the benthos using stainless steel threaded rods. Five CAUs were deployed at each site in Timor-Leste. Calcareous organisms, primarily crustose coralline algae and encrusting corals, recruit to these CAUs and accrete/calcify carbonate skeletons during the deployment perios (~2-3 years). Once recovered from the seafloor, the CAUs are processed to provide estimates of net calcification. CAUs have been deployed and replaced at sites in Timor-Leste, in accordance with protocols developed by Price et al. 2012.

Sources

NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Assembly, Deployment, and Recovery Standard Operating Procedure (2015)

CC ID: 569532
Contact Type: Organization
Contact Name: Pacific Islands Fisheries Science Center, PIFSC
Publish Date: 2015-08-01
Citation URL: http://data.nodc.noaa.gov/coris/data/NOAA/nmfs/pifsc/cred/cau/CAU1_Assembly_Deployment_Recovery_CRED_SOP_2015.pdf

NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Laboratory Analysis Standard Operating Procedure (2015)

CC ID: 569533
Contact Type: Organization
Contact Name: Pacific Islands Fisheries Science Center, PIFSC
Publish Date: 2015-09-01
Citation URL: http://data.nodc.noaa.gov/coris/data/NOAA/nmfs/pifsc/cred/cau/CAU2_Sample%20Processing_CRED_SOP_2015.pdf

Price NN, Martz TR, Brainard RE, Smith JE (2012) Diel Variability in Seawater pH Relates to Calcification and Benthic Community Structure on Coral Reefs. PLoS ONE 7(8): e43843. doi:10.1371/journal.pone.0043843

CC ID: 569534
Contact Name: Scripps Institution of Oceanography, University of California San Diego
Publish Date: 2012-08-28
Citation URL: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0043843

Process Steps

Process Step 1

CC ID: 569535
Description:

CALCIFICATION ACCRETION UNIT (CAU) ASSEMBLY, DEPLOYMENT and RECOVERY

CAUs are composed of two 10 x 10 centimeter (cm) flat, square, gray PVC plates, stacked 1 cm apart.

Five CAU units are deployed per site on individual stainless steel stakes driven into the substrate. The units are deployed in a clustered group with the spacing between each unit being 0.5 to 5 meters. CAU sites are situated at an average depth of 15 meters. Units are placed so the CAU plates are ~10 cm above the surface substrate and the top of the CAU plate is parallel to the surface of the water.

CAU units are recovered from the seafloor 2 to 3 years after deployment. Refer to the data files for the individual CAU site to determine the exact deployment time of a specific unit.

Refer to the NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Assembly, Deployment, and Recovery Standard Operating Procedure (2015) for a detailed description of the protocols.

Process Date/Time: 2015-08-01 00:00:00
Process Contact: Young, Charles W
Email Address: charles.young@noaa.gov
Source: NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Assembly, Deployment, and Recovery Standard Operating Procedure (2015)

Process Step 2

CC ID: 569536
Description:

CALCIFICATION ACCRETION UNIT (CAU) LABORATORY ANALYSIS

Once CAU units are recovered they are disassembled and each plate is rinsed with saltwater to remove loose sediment, sand and mobile fauna. The plates are photographed and individual images are captured of the Upper Plate, Top Side; Upper Plate, Bottom Side; Lower Plate, Top Side; Lower Plate, Bottom Side. These images are later analyzed to determine benthic composition on each plate surface. If pieces of calcified material fall off during the photographing process, the pieces are retained with the plate for the dissolution process.

After the plates have been photographed they are rinsed with fresh water and placed on a pre-weighed drying plate along with any dislodged pieces, and left to begin drying at room temperature for 24 hours and TO allow excess water to be removed. The drying plate assembly is then placed in the oven to dry at 60 degrees Celsius. If the difference in weights is less than 0.2 grams, the plates are considered dry and no further drying/weighing is required. If the difference in weights is greater than 0.2 grams the drying/reweighing process is repeated for as many 24-hour cycles as needed to obtain a difference of less than 0.2 grams. It should be noted that since the initial sample processing in 2012, the standard weighing protocol has since been improved such that the acceptable weight difference is 0.1 grams.

Once the plates are dry, each plate is placed in 5% Hydrocholoric Acid (HCl) solution to dissolve the calcium carbonate materials. The plate is scraped to remove all materials and rinsed with the 5% HCl solution. The plate is then placed in the oven at 60 degrees Celsius to dry for 24 hours before the dry plate is obtained. Through the entire dissolution process all 5% HCl rinse solution is preserved for later filtering.

The 5% HCl rinse process is repeated on all materials removed from the plate until the dissolution process has stopped and all calcium carbonate material has been removed. The fleshy material and used 5% HCl rinse materials are then separated using a vacuum filtration pump by pouring the solution into a Buchner funnel, mesh layer and pre-weighed filter paper. The filter paper and fleshy material are then dried and weighed to determine the weight of fleshy algae present on each plate. The weight of calcified materials on each plate is determined by difference, where the initial weight of the dried plate (prior to the dissolution process) minus the fleshy material weight equals the weight of carbonate material dissolved. The resulting calcification rates are reported in units of grams/area/time.

Refer to the NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Laboratory Analysis Standard Operating Procedure (2015) for a detailed description of the protocols.

Process Date/Time: 2015-09-01 00:00:00
Process Contact: Young, Charles W
Email Address: charles.young@noaa.gov
Source: NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Laboratory Analysis Standard Operating Procedure (2015)

Child Items

Rubric scores updated every 15m

Rubric Score Type Title
Entity (ENT) View: V_CALCIFICATION_RATE_TIMOR

Catalog Details

Catalog Item ID: 46162
Metadata Record Created By: Annette M DesRochers
Metadata Record Created: 2017-06-02 03:20+0000
Metadata Record Last Modified By: SysAdmin InPortAdmin
Metadata Record Last Modified: 2020-08-03 16:13+0000
Metadata Record Published: 2018-01-16
Owner Org: PIFSC
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Next Review Date: 2019-01-17